Chemosensory signalling pathways involved in sensing of amino acids by the ghrelin cell.

Abstract:

:Taste receptors on enteroendocrine cells sense nutrients and transmit signals that control gut hormone release. This study aimed to investigate the amino acid (AA) sensing mechanisms of the ghrelin cell in a gastric ghrelinoma cell line, tissue segments and mice. Peptone and specific classes of amino acids stimulate ghrelin secretion in the ghrelinoma cell line. Sensing of L-Phe occurs via the CaSR, monosodium glutamate via the TAS1R1-TAS1R3 while L-Ala and peptone act via 2 different amino acid taste receptors: CaSR &TAS1R1-TAS1R3 and CaSR &GPRC6A, respectively. The stimulatory effect of peptone on ghrelin release was mimicked ex vivo in gastric but not in jejunal tissue segments, where peptone inhibited ghrelin release. The latter effect could not be blocked by receptor antagonists for CCK, GLP-1 or somatostatin. In vivo, plasma ghrelin levels were reduced both upon intragastric (peptone or L-Phe) or intravenous (L-Phe) administration, indicating that AA- sensing is not polarized and is due to inhibition of ghrelin release from the stomach or duodenum respectively. In conclusion, functional AA taste receptors regulate AA-induced ghrelin release in vitro. The effects differ between stomach and jejunum but these local nutrient sensing mechanisms are overruled in vivo by indirect mechanisms inhibiting ghrelin release.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Vancleef L,Van Den Broeck T,Thijs T,Steensels S,Briand L,Tack J,Depoortere I

doi

10.1038/srep15725

subject

Has Abstract

pub_date

2015-10-29 00:00:00

pages

15725

issn

2045-2322

pii

srep15725

journal_volume

5

pub_type

杂志文章
  • Calibrating the tree of vipers under the fossilized birth-death model.

    abstract::Scaling evolutionary trees to time is essential for understanding the origins of clades. Recently developed methods allow including the entire fossil record known for the group of interest and eliminated the need for specifying prior distributions for node ages. Here we apply the fossilized birth-death (FBD) approach ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41290-2

    authors: Šmíd J,Tolley KA

    更新日期:2019-04-02 00:00:00

  • Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis.

    abstract::During symbiosis, organisms use a range of metabolic and protein-based signals to communicate. Of these protein signals, one class is defined as 'effectors', i.e., small secreted proteins (SSPs) that cause phenotypical and physiological changes in another organism. To date, protein-based effectors have been described ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-00400-8

    authors: Plett JM,Yin H,Mewalal R,Hu R,Li T,Ranjan P,Jawdy S,De Paoli HC,Butler G,Burch-Smith TM,Guo HB,Ju Chen C,Kohler A,Anderson IC,Labbé JL,Martin F,Tuskan GA,Yang X

    更新日期:2017-03-23 00:00:00

  • Dibutyl phthalate alters the metabolic pathways of microbes in black soils.

    abstract::Dibutyl phthalate (DBP) is well known as a high-priority pollutant. This study explored the impacts of DBP on the metabolic pathways of microbes in black soils in the short term (20 days). The results showed that the microbial communities were changed in black soils with DBP. In nitrogen cycling, the abundances of the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21030-8

    authors: Xu W,You Y,Wang Z,Chen W,Zeng J,Zhao X,Su Y

    更新日期:2018-02-08 00:00:00

  • In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles.

    abstract::Capable of generating plasmonic and other effects, gold nanostructures can offer a variety of diagnostic and therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements for toxicity levels and surface conditioning. Laser-synth...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48748-3

    authors: Bailly AL,Correard F,Popov A,Tselikov G,Chaspoul F,Appay R,Al-Kattan A,Kabashin AV,Braguer D,Esteve MA

    更新日期:2019-09-09 00:00:00

  • Auditory Neuropathy after Damage to Cochlear Spiral Ganglion Neurons in Mice Resulting from Conditional Expression of Diphtheria Toxin Receptors.

    abstract::Auditory neuropathy (AN) is a hearing disorder characterized by normal cochlear amplification to sound but poor temporal processing and auditory perception in noisy backgrounds. These deficits likely result from impairments in auditory neural synchrony; such dyssynchrony of the neural responses has been linked to demy...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06600-6

    authors: Pan H,Song Q,Huang Y,Wang J,Chai R,Yin S,Wang J

    更新日期:2017-07-25 00:00:00

  • Isolation and characterization of a multifunctional flavonoid glycosyltransferase from Ornithogalum caudatum with glycosidase activity.

    abstract::Glycosyltransferases (GTs) are bidirectional biocatalysts catalyzing the glycosylation of diverse molecules. However, the extensive applications of GTs in glycosides formation are limited due to their requirements of expensive nucleotide diphosphate (NDP)-sugars or NDP as the substrates. Here, in an effort to characte...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24277-3

    authors: Yuan S,Yin S,Liu M,Kong JQ

    更新日期:2018-04-12 00:00:00

  • Generation of Customizable Micro-wavy Pattern through Grayscale Direct Image Lithography.

    abstract::With the increasing amount of research work in surface studies, a more effective method of producing patterned microstructures is highly desired due to the geometric limitations and complex fabricating process of current techniques. This paper presents an efficient and cost-effective method to generate customizable mi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21621

    authors: He R,Wang S,Andrews G,Shi W,Liu Y

    更新日期:2016-02-23 00:00:00

  • Publisher Correction: Mixed-Dimensional Naphthylmethylammonium-Methylammonium Lead Iodide Perovskites with Improved Thermal Stability.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-65027-8

    authors: Chaudhary B,Koh TM,Febriansyah B,Bruno A,Mathews N,Mhaisalkar SG,Soci C

    更新日期:2020-05-20 00:00:00

  • Characterization of A Bifunctional Synthetic RNA Aptamer and A Truncated Form for Ability to Inhibit Growth of Non-Small Cell Lung Cancer.

    abstract::An in vitro-transcribed RNA aptamer (trans-RA16) that targets non-small cell lung cancer (NSCLC) was previously identified through in vivo SELEX. Trans-RA16 can specifically target and inhibit human NCI-H460 cells in vitro and xenograft tumors in vivo. Here, in a follow-up study, we obtained a chemically-synthesized v...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-55280-x

    authors: Wang H,Qin M,Liu R,Ding X,Chen ISY,Jiang Y

    更新日期:2019-12-11 00:00:00

  • Iron biofortification and availability in the mycelial biomass of edible and medicinal basidiomycetes cultivated in sugarcane molasses.

    abstract::Basidiomycetes can bioaccumulate high iron contents, but there are few studies on iron availability from the mycelial biomass in order to support their use as an iron-enriched fungal food. This study aimed to evaluate the in vitro iron bioaccumulation and availability in the mycelial biomass of edible and medicinal ba...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69699-0

    authors: Scheid SS,Faria MGI,Velasquez LG,do Valle JS,Gonçalves AC Jr,Dragunski DC,Colauto NB,Linde GA

    更新日期:2020-07-30 00:00:00

  • Interferon gamma-induced protein 10 is associated with insulin resistance and incident diabetes in patients with nonalcoholic fatty liver disease.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is an important risk factor for the development of type 2 diabetes mellitus. Interferon gamma-induced protein 10 (IP-10), a proinflammatory chemokine, plays a crucial role in inflammatory diseases. This cross-sectional pilot study investigated whether circulating IP-10 is assoc...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1038/srep10096

    authors: Chang CC,Wu CL,Su WW,Shih KL,Tarng DC,Chou CT,Chen TY,Kor CT,Wu HM

    更新日期:2015-05-11 00:00:00

  • Tyrosine kinase receptor TIE-1 mediates platinum resistance by promoting nucleotide excision repair in ovarian cancer.

    abstract::Platinum resistance is one of the most challenging problems in ovarian cancer treatment. High-throughput functional siRNA screening identified tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE-1) as a gene that confers cells resistant to cisplatin. Conversely enforced over-expression of TIE-1 was va...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31069-2

    authors: Ishibashi M,Toyoshima M,Zhang X,Hasegawa-Minato J,Shigeta S,Usui T,Kemp CJ,Grandori C,Kitatani K,Yaegashi N

    更新日期:2018-09-04 00:00:00

  • Glucokinase intrinsically regulates glucose sensing and glucagon secretion in pancreatic alpha cells.

    abstract::The secretion of glucagon by pancreatic alpha cells is regulated by a number of external and intrinsic factors. While the electrophysiological processes linking a lowering of glucose concentrations to an increased glucagon release are well characterized, the evidence for the identity and function of the glucose sensor...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76863-z

    authors: Moede T,Leibiger B,Vaca Sanchez P,Daré E,Köhler M,Muhandiramlage TP,Leibiger IB,Berggren PO

    更新日期:2020-11-19 00:00:00

  • Intraperitoneal administration of the anti-IL-23 antibody prevents the establishment of intestinal nematodes in mice.

    abstract::Previous studies have established that an increased Th-9 response creates a hostile environment for nematode parasites. Given that IL-23, a cytokine required for maintenance of the IL-17-secreting phenotype, has inhibitory effects on IL-9 production, we hypothesized that reducing circulating IL-23 by treatment with an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26194-x

    authors: Gomez-Samblas M,Bernal D,Bolado-Ortiz A,Vilchez S,Bolás-Fernández F,Espino AM,Trelis M,Osuna A

    更新日期:2018-05-17 00:00:00

  • Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion.

    abstract::Neuropathic pain is a complex, chronic pain state that often accompanies tissue damage, inflammation or injury of the nervous system. However the underlying molecular mechanisms still remain unclear. Here, we showed that CXCL12 and CXCR4 were upregulated in the dorsal root ganglion (DRG) after chronic compression of D...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05954-1

    authors: Yu Y,Huang X,Di Y,Qu L,Fan N

    更新日期:2017-07-18 00:00:00

  • Risk of stroke in patients with rheumatism: a nationwide longitudinal population-based study.

    abstract::The aim of this study was to investigate rheumatoid arthritis (RA), and systemic lupus erythematous (SLE) as risk factors for stroke. The study was analyzed by Using the Taiwan Longitudinal Health Insurance Database 2005 (LHID2005), this cohort study investigated patients with a recorded diagnosis of RA (N = 6114), an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05110

    authors: Liou TH,Huang SW,Lin JW,Chang YS,Wu CW,Lin HW

    更新日期:2014-06-05 00:00:00

  • Identification of wheat stress-responding genes and TaPR-1-1 function by screening a cDNA yeast library prepared following abiotic stress.

    abstract::Abiotic stress significantly impacts growth and yield of crop plants. It is imperative for crop improvement to discover and utilize stress-tolerant functional genes. In this study, genes responding to abiotic stresses, such as freezing, salt and osmotic stress, were screened from a cDNA yeast library that was construc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37859-y

    authors: Wang J,Mao X,Wang R,Li A,Zhao G,Zhao J,Jing R

    更新日期:2019-01-15 00:00:00

  • Using stable isotopes to analyse extinction risks and reintroduction opportunities of native species in invaded ecosystems.

    abstract::Invasive non-native species have pervasive impacts on native biodiversity, including population extirpations and species extinctions. Identifying reasons why a population of a native species is extirpated following an invasion often relies on literature-based results of anecdotal observations. The well-established sch...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78328-9

    authors: Haubrock PJ,Balzani P,Britton JR,Haase P

    更新日期:2020-12-10 00:00:00

  • Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture.

    abstract::The extensive redundancy in the use of invasion ligands by Plasmodium falciparum, and its unique ability to switch between invasion pathways have hampered vaccine development. P. falciparum strains Dd2 and W2mef have been shown to change from sialic acid (SA)-dependent to SA-independent phenotypes when selected on neu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24218-0

    authors: Awandare GA,Nyarko PB,Aniweh Y,Ayivor-Djanie R,Stoute JA

    更新日期:2018-04-10 00:00:00

  • Artificial modulation of cell width significantly affects the division time of Escherichia coli.

    abstract::Bacterial cells have characteristic spatial and temporal scales. For instance, Escherichia coli, the typical rod-shaped bacteria, always maintains a relatively constant cell width and cell division time. However, whether the external physical perturbation of cell width has an impact on cell division time remains large...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74778-3

    authors: Liang B,Quan B,Li J,Loton C,Bredeche MF,Lindner AB,Xu L

    更新日期:2020-10-20 00:00:00

  • Acquired Enamel Pellicle Engineered Peptides: Effects on Hydroxyapatite Crystal Growth.

    abstract::The aim of this study was to test the hypothesis that duplication/hybridization of functional domains of naturally occurring pellicle peptides amplified the inhibitory effect of hydroxyapatite crystal growth, which is related to enamel remineralization and dental calculus formation. Histatin 3, statherin, their functi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21854-4

    authors: Valente MT,Moffa EB,Crosara KTB,Xiao Y,de Oliveira TM,Machado MAAM,Siqueira WL

    更新日期:2018-02-28 00:00:00

  • In vitro hematological and in vivo vasoactivity assessment of dextran functionalized graphene.

    abstract::The intravenous, intramuscular or intraperitoneal administration of water solubilized graphene nanoparticles for biomedical applications will result in their interaction with the hematological components and vasculature. Herein, we have investigated the effects of dextran functionalized graphene nanoplatelets (GNP-Dex...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02584

    authors: Chowdhury SM,Kanakia S,Toussaint JD,Frame MD,Dewar AM,Shroyer KR,Moore W,Sitharaman B

    更新日期:2013-01-01 00:00:00

  • Oceanic vortex mergers are not isolated but influenced by the β-effect and surrounding eddies.

    abstract::Oceanic vortices are ubiquitous in the ocean. They dominate the sub-inertial energy spectrum, and their dynamics is key for the evolution of the water column properties. The merger of two like-signed coherent vortices, which ultimately results in the formation of a larger vortex, provides an efficient mechanism for th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59800-y

    authors: de Marez C,Carton X,L'Hégaret P,Meunier T,Stegner A,Le Vu B,Morvan M

    更新日期:2020-02-19 00:00:00

  • Integration of fish culture and poultry rearing in transplanted rice for nutritional security in smallholder farms.

    abstract::Agriculture provides livelihood for 65% of Nepal's population contributing for 27% of its GDP. Smallholder farms constitute 60% of farming segment. Distress farming, with inadequate minimum support price, subsidies and inadequate revenue generation force 29% of the general population to be malnourished. Farming system...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67657-4

    authors: Ramanathan K,Sangeeviraman V,Chandrahasan P,Chaudhary BN,Ramachandra SS

    更新日期:2020-06-29 00:00:00

  • Lung Clearance Index and Quantitative Computed Tomography of Post-Infectious Bronchiolitis Obliterans in Infants.

    abstract::Post-infectious bronchiolitis obliterans (BO) could be diagnosed via spirometry and chest computed tomography (CT); however, these tests are limited in infants. We aimed to evaluate the utility of lung clearance index (LCI) and air-trapping lung volume from chest CT in infants. This prospective study included 20 infan...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15330-8

    authors: Kim YH,Shin HJ,Sol IS,Kim SY,Kim JD,Yoon H,Kim KW,Kim MJ,Lee MJ,Sohn MH

    更新日期:2017-11-09 00:00:00

  • WRINKLED1 transcription factor orchestrates the regulation of carbon partitioning for C18:1 (oleic acid) accumulation in Siberian apricot kernel.

    abstract::WRINKLED1 (WRI1), an APETALA2 (AP2)-type transcription factor, has been shown to be required for the regulation of carbon partitioning into fatty acid (FA) synthesis in plant seeds. To our knowledge, the regulatory network of WRI1 remains unknown in Prunus sibirica kernel (PSK), a novel woody biodiesel feedstock in Ch...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39236-9

    authors: Deng S,Mai Y,Shui L,Niu J

    更新日期:2019-02-25 00:00:00

  • Antimicrobial and inflammatory properties of South African clinical Lactobacillus isolates and vaginal probiotics.

    abstract::Bacterial vaginosis (BV) causes genital inflammation and increased HIV acquisition risk. The standard-of-care for BV, antibiotic therapy, is associated with high recurrence rates. Probiotics may improve treatment outcomes, although substantial heterogeneity in efficacy has been observed during clinical trials. To eval...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/s41598-018-38253-4

    authors: Chetwin E,Manhanzva MT,Abrahams AG,Froissart R,Gamieldien H,Jaspan H,Jaumdally SZ,Barnabas SL,Dabee S,Happel AU,Bowers D,Davids L,Passmore JS,Masson L

    更新日期:2019-02-13 00:00:00

  • Direct Pore Binding as a Mechanism for Isoflurane Inhibition of the Pentameric Ligand-gated Ion Channel ELIC.

    abstract::Pentameric ligand-gated ion channels (pLGICs) are targets of general anesthetics, but molecular mechanisms underlying anesthetic action remain debatable. We found that ELIC, a pLGIC from Erwinia chrysanthemi, can be functionally inhibited by isoflurane and other anesthetics. Structures of ELIC co-crystallized with iso...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13833

    authors: Chen Q,Kinde MN,Arjunan P,Wells MM,Cohen AE,Xu Y,Tang P

    更新日期:2015-09-08 00:00:00

  • Calmodulin is responsible for Ca2+-dependent regulation of TRPA1 Channels.

    abstract::TRPA1 is a Ca2+-permeable ion channel involved in many sensory disorders such as pain, itch and neuropathy. Notably, the function of TRPA1 depends on Ca2+, with low Ca2+ potentiating and high Ca2+ inactivating TRPA1. However, it remains unknown how Ca2+ exerts such contrasting effects. Here, we show that Ca2+ regulate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45098

    authors: Hasan R,Leeson-Payne AT,Jaggar JH,Zhang X

    更新日期:2017-03-23 00:00:00

  • A Comparative Metabolomics Approach Reveals Early Biomarkers for Metabolic Response to Acute Myocardial Infarction.

    abstract::Discovery of novel biomarkers is critical for early diagnosis of acute coronary syndrome (ACS). Serum metabolite profiling of ST-elevation myocardial infarction (STEMI), unstable angina (UA) and healthy controls was performed using gas chromatography mass spectrometry (GC/MS), solid-phase microextraction coupled to ga...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36359

    authors: Ali SE,Farag MA,Holvoet P,Hanafi RS,Gad MZ

    更新日期:2016-11-08 00:00:00