Consequences of Cryopreservation in Diverse Natural Isolates of Saccharomyces cerevisiae.

Abstract:

:Experimental evolution allows the observation of change over time as laboratory populations evolve in response to novel, controlled environments. Microbial evolution experiments take advantage of cryopreservation to archive experimental populations in glycerol media, creating a frozen, living "fossil" record. Prior research with Escherichia coli has shown that cryopreservation conditions can affect cell viability and that allele frequencies across the genome can change in response to a freeze-thaw event. We expand on these observations by characterizing fitness and genomic consequences of multiple freeze-thaw cycles in diploid yeast populations. Our study system is a highly recombinant Saccharomyces cerevisiae population (SGRP-4X) that harbors standing genetic variation that cryopreservation may threaten. We also investigate the four parental isogenic strains crossed to create the SGRP-4X. We measure cell viability over five consecutive freeze-thaw cycles; whereas we find that viability increases over time in the evolved recombinant populations, we observe no such viability improvements in the parental strains. We also collect genome-wide sequence data from experimental populations initially, after one freeze-thaw, and after five freeze-thaw cycles. In the recombinant evolved populations, we find a region of significant allele frequency change on chromosome 15 containing the ALR1 gene. In the parental strains, we find little evidence for new mutations. We conclude that cryopreserving yeast populations with standing genetic variation may have both phenotypic and genomic consequences, though the same cryopreservation practices may have only small impacts on populations with little or no initial variation.

journal_name

Genome Biol Evol

authors

Wing KM,Phillips MA,Baker AR,Burke MK

doi

10.1093/gbe/evaa121

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

1302-1312

issue

8

issn

1759-6653

pii

5865864

journal_volume

12

pub_type

杂志文章
  • Highly variable recombinational landscape modulates efficacy of natural selection in birds.

    abstract::Determining the rate of protein evolution and identifying the causes of its variation across the genome are powerful ways to understand forces that are important for genome evolution. By using a multitissue transcriptome data set from great tit (Parus major), we analyzed patterns of molecular evolution between two pas...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evu157

    authors: Gossmann TI,Santure AW,Sheldon BC,Slate J,Zeng K

    更新日期:2014-08-01 00:00:00

  • Gene frequency distributions reject a neutral model of genome evolution.

    abstract::Evolution of prokaryotes involves extensive loss and gain of genes, which lead to substantial differences in the gene repertoires even among closely related organisms. Through a wide range of phylogenetic depths, gene frequency distributions in prokaryotic pangenomes bear a characteristic, asymmetrical U-shape, with a...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evt002

    authors: Lobkovsky AE,Wolf YI,Koonin EV

    更新日期:2013-01-01 00:00:00

  • Horizontal Gene Transfer in Five Parasite Plant Species in Orobanchaceae.

    abstract::We sequenced genomes of five parasite species in family Orobanchaceae to explore the evolutionary role of horizontal gene transfer in plants. Orobanche minor and Aeginetia indica are obligate parasites with no photosynthetic activity, whereas the other three (Pedicularis keiskei, Phtheirospermum japonicum, and Melampy...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evy219

    authors: Kado T,Innan H

    更新日期:2018-12-01 00:00:00

  • The mitochondrial genome of Paraminabea aldersladei (Cnidaria: Anthozoa: Octocorallia) supports intramolecular recombination as the primary mechanism of gene rearrangement in octocoral mitochondrial genomes.

    abstract::Sequencing of the complete mitochondrial genome of the soft coral Paraminabea aldersladei (Alcyoniidae) revealed a unique gene order, the fifth mt gene arrangement now known within the cnidarian subclass Octocorallia. At 19,886 bp, the mt genome of P. aldersladei is the second largest known for octocorals; its gene co...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evs074

    authors: Brockman SA,McFadden CS

    更新日期:2012-01-01 00:00:00

  • Network-Based Analysis of eQTL Data to Prioritize Driver Mutations.

    abstract::In clonal systems, interpreting driver genes in terms of molecular networks helps understanding how these drivers elicit an adaptive phenotype. Obtaining such a network-based understanding depends on the correct identification of driver genes. In clonal systems, independent evolved lines can acquire a similar adaptive...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evw010

    authors: De Maeyer D,Weytjens B,De Raedt L,Marchal K

    更新日期:2016-01-23 00:00:00

  • Intergenic transposable elements are not randomly distributed in bacteria.

    abstract::Insertion sequences (ISs) are mobile genetic elements in bacterial genomes. In general, intergenic IS elements are probably less deleterious for their hosts than intragenic ISs, simply because they have a lower likelihood of disrupting native genes. However, since promoters, Shine-Dalgarno sequences, and transcription...

    journal_title:Genome biology and evolution

    pub_type: 信件

    doi:10.1093/gbe/evq040

    authors: Plague GR

    更新日期:2010-01-01 00:00:00

  • The potential role of sexual conflict and sexual selection in shaping the genomic distribution of Mito-nuclear genes.

    abstract::Mitochondrial interactions with the nuclear genome represent one of life's most important co-evolved mutualisms. In many organisms, mitochondria are maternally inherited, and in these cases, co-transmission between the mitochondrial and nuclear genes differs across different parts of the nuclear genome, with genes on ...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evu063

    authors: Dean R,Zimmer F,Mank JE

    更新日期:2014-05-01 00:00:00

  • Paternally transmitted mitochondria express a new gene of potential viral origin.

    abstract::Mitochondrial ORFans (open reading frames having no detectable homology and with unknown function) were discovered in bivalve molluscs with doubly uniparental inheritance (DUI) of mitochondria. In these animals, two mitochondrial lineages are present, one transmitted through eggs (F-type), the other through sperm (M-t...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evu021

    authors: Milani L,Ghiselli F,Maurizii MG,Nuzhdin SV,Passamonti M

    更新日期:2014-02-01 00:00:00

  • Lineage-Specific Expression Divergence in Grasses Is Associated with Male Reproduction, Host-Pathogen Defense, and Domestication.

    abstract::Poaceae (grasses) is an agriculturally important and widely distributed family of plants with extraordinary phenotypic diversity, much of which was generated under recent lineage-specific evolution. Yet, little is known about the genes and functional modules involved in the lineage-specific divergence of grasses. Here...

    journal_title:Genome biology and evolution

    pub_type: 信件

    doi:10.1093/gbe/evy245

    authors: Assis R

    更新日期:2019-01-01 00:00:00

  • The Limits to Estimating Population-Genetic Parameters with Temporal Data.

    abstract::The ability to obtain genome-wide sequences of very large numbers of individuals from natural populations raises questions about optimal sampling designs and the limits to extracting information on key population-genetic parameters from temporal-survey data. Methods are introduced for evaluating whether observed tempo...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evaa056

    authors: Lynch M,Ho WC

    更新日期:2020-04-01 00:00:00

  • Conserved meiotic machinery in Glomus spp., a putatively ancient asexual fungal lineage.

    abstract::Arbuscular mycorrhizal fungi (AMF) represent an ecologically important and evolutionarily intriguing group of symbionts of land plants, currently thought to have propagated clonally for over 500 Myr. AMF produce multinucleate spores and may exchange nuclei through anastomosis, but meiosis has never been observed in th...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evr089

    authors: Halary S,Malik SB,Lildhar L,Slamovits CH,Hijri M,Corradi N

    更新日期:2011-01-01 00:00:00

  • Regulation of Large Number of Weak Targets-New Insights from Twin-microRNAs.

    abstract::Each animal microRNA (miRNA) targets many genes for repression. Down-regulation of most of these targets is weak and has no detectable individual phenotypic effect. Whether this extensive weak repression is biologically relevant is a central issue in the debate on miRNA functionality. In the "small (target) pool" view...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evy079

    authors: Zhao Y,Lin P,Liufu Z,Yang H,Lyu Y,Shen X,Wu CI,Tang T

    更新日期:2018-04-01 00:00:00

  • Genome Sequencing of the Behavior Manipulating Virus LbFV Reveals a Possible New Virus Family.

    abstract::Parasites are sometimes able to manipulate the behavior of their hosts. However, the molecular cues underlying this phenomenon are poorly documented. We previously reported that the parasitoid wasp Leptopilina boulardi which develops from Drosophila larvae is often infected by an inherited DNA virus. In addition to be...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evw277

    authors: Lepetit D,Gillet B,Hughes S,Kraaijeveld K,Varaldi J

    更新日期:2016-12-01 00:00:00

  • Adaptive Prediction Emerges Over Short Evolutionary Time Scales.

    abstract::Adaptive prediction is a capability of diverse organisms, including microbes, to sense a cue and prepare in advance to deal with a future environmental challenge. Here, we investigated the timeframe over which adaptive prediction emerges when an organism encounters an environment with novel structure. We subjected yea...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evx116

    authors: López García de Lomana A,Kaur A,Turkarslan S,Beer KD,Mast FD,Smith JJ,Aitchison JD,Baliga NS

    更新日期:2017-06-01 00:00:00

  • Comprehensive Genome-Wide Classification Reveals That Many Plant-Specific Transcription Factors Evolved in Streptophyte Algae.

    abstract::Plant genomes encode many lineage-specific, unique transcription factors. Expansion of such gene families has been previously found to coincide with the evolution of morphological complexity, although comparative analyses have been hampered by severe sampling bias. Here, we make use of the recently increased availabil...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evx258

    authors: Wilhelmsson PKI,Mühlich C,Ullrich KK,Rensing SA

    更新日期:2017-12-01 00:00:00

  • Ampliconic Genes on the Great Ape Y Chromosomes: Rapid Evolution of Copy Number but Conservation of Expression Levels.

    abstract::Multicopy ampliconic gene families on the Y chromosome play an important role in spermatogenesis. Thus, studying their genetic variation in endangered great ape species is critical. We estimated the sizes (copy number) of nine Y ampliconic gene families in population samples of chimpanzee, bonobo, and orangutan with d...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evaa088

    authors: Vegesna R,Tomaszkiewicz M,Ryder OA,Campos-Sánchez R,Medvedev P,DeGiorgio M,Makova KD

    更新日期:2020-06-01 00:00:00

  • Multiple Roots of Fruiting Body Formation in Amoebozoa.

    abstract::Establishment of multicellularity represents a major transition in eukaryote evolution. A subgroup of Amoebozoa, the dictyosteliids, has evolved a relatively simple aggregative multicellular stage resulting in a fruiting body supported by a stalk. Protosteloid amoeba, which are scattered throughout the amoebozoan tree...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evy011

    authors: Hillmann F,Forbes G,Novohradská S,Ferling I,Riege K,Groth M,Westermann M,Marz M,Spaller T,Winckler T,Schaap P,Glöckner G

    更新日期:2018-02-01 00:00:00

  • Recurrent Loss, Horizontal Transfer, and the Obscure Origins of Mitochondrial Introns in Diatoms (Bacillariophyta).

    abstract::We sequenced mitochondrial genomes from five diverse diatoms (Toxarium undulatum, Psammoneis japonica, Eunotia naegelii, Cylindrotheca closterium, and Nitzschia sp.), chosen to fill important phylogenetic gaps and help us characterize broadscale patterns of mitochondrial genome evolution in diatoms. Although gene cont...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evy103

    authors: Guillory WX,Onyshchenko A,Ruck EC,Parks M,Nakov T,Wickett NJ,Alverson AJ

    更新日期:2018-06-01 00:00:00

  • Uterine gene expression in the live-bearing lizard, Chalcides ocellatus, reveals convergence of squamate reptile and mammalian pregnancy mechanisms.

    abstract::Although the morphological and physiological changes involved in pregnancy in live-bearing reptiles are well studied, the genetic mechanisms that underlie these changes are not known. We used the viviparous African Ocellated Skink, Chalcides ocellatus, as a model to identify a near complete gene expression profile ass...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evs013

    authors: Brandley MC,Young RL,Warren DL,Thompson MB,Wagner GP

    更新日期:2012-01-01 00:00:00

  • Spatial dynamics of evolving dosage compensation in a young sex chromosome system.

    abstract::The loss of Y-linked genes during sex chromosome evolution creates a potentially deleterious low gene dosage in males. Recent studies have reported different strategies of dosage compensation. Unfortunately, most of these studies investigated taxa with comparatively old sex chromosome systems, which may limit insights...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evv013

    authors: Schultheiß R,Viitaniemi HM,Leder EH

    更新日期:2015-01-23 00:00:00

  • Comparative Transcriptomics across Nematode Life Cycles Reveal Gene Expression Conservation and Correlated Evolution in Adjacent Developmental Stages.

    abstract::Nematodes are highly abundant animals with diverse habitats and lifestyles. Some are free living whereas others parasitize animals or plants, and among the latter, infection abilities change across developmental stages to infect hosts and complete life cycles. To determine the relationship between transcriptome evolut...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evaa110

    authors: Lu MR,Lai CK,Liao BY,Tsai IJ

    更新日期:2020-07-01 00:00:00

  • Pattern of DNA Methylation in Daphnia: Evolutionary Perspective.

    abstract::DNA methylation is an evolutionary ancient epigenetic modification that is phylogenetically widespread. Comparative studies of the methylome across a diverse range of non-conventional and conventional model organisms is expected to help reveal how the landscape of DNA methylation and its functions have evolved. Here, ...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evy155

    authors: Kvist J,Gonçalves Athanàsio C,Shams Solari O,Brown JB,Colbourne JK,Pfrender ME,Mirbahai L

    更新日期:2018-08-01 00:00:00

  • A linear molecule with two large inverted repeats: the mitochondrial genome of the stramenopile Proteromonas lacertae.

    abstract::Mitochondrial evolution has given rise to a complex array of organelles, ranging from classical aerobic mitochondria to mitochondrial remnants known as hydrogenosomes and mitosomes. The latter are found in anaerobic eukaryotes, and these highly derived organelles often retain only scant evidence of their mitochondrial...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evq015

    authors: Pérez-Brocal V,Shahar-Golan R,Clark CG

    更新日期:2010-07-12 00:00:00

  • Evolutionary Rate Heterogeneity of Primary and Secondary Metabolic Pathway Genes in Arabidopsis thaliana.

    abstract::Primary metabolism is essential to plants for growth and development, and secondary metabolism helps plants to interact with the environment. Many plant metabolites are industrially important. These metabolites are produced by plants through complex metabolic pathways. Lack of knowledge about these pathways is hinderi...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evv217

    authors: Mukherjee D,Mukherjee A,Ghosh TC

    更新日期:2015-11-10 00:00:00

  • The Combined Effect of Oseltamivir and Favipiravir on Influenza A Virus Evolution.

    abstract::Influenza virus inflicts a heavy death toll annually and resistance to existing antiviral drugs has generated interest in the development of agents with novel mechanisms of action. Favipiravir is an antiviral drug that acts by increasing the genome-wide mutation rate of influenza A virus (IAV). Potential synergistic b...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evx138

    authors: Ormond L,Liu P,Matuszewski S,Renzette N,Bank C,Zeldovich K,Bolon DN,Kowalik TF,Finberg RW,Jensen JD,Wang JP

    更新日期:2017-07-01 00:00:00

  • Scanning for the signatures of positive selection for human-specific insertions and deletions.

    abstract::Human-specific small insertions and deletions (HS indels, with lengths <100 bp) are reported to be ubiquitous in the human genome. However, whether these indels contribute to human-specific traits remains unclear. Here we employ a modified McDonald-Kreitman (MK) test and a combinatorial population genetics approach to...

    journal_title:Genome biology and evolution

    pub_type: 信件

    doi:10.1093/gbe/evp041

    authors: Chen CH,Chuang TJ,Liao BY,Chen FC

    更新日期:2009-10-20 00:00:00

  • Comparative Genomic Analysis of Acanthamoeba Endosymbionts Highlights the Role of Amoebae as a "Melting Pot" Shaping the Rickettsiales Evolution.

    abstract::Amoebae have been considered as a genetic "melting pot" for its symbionts, facilitating genetic exchanges of the bacteria that co-inhabit the same host. To test the "melting pot" hypothesis, we analyzed six genomes of amoeba endosymbionts within Rickettsiales, four of which belong to Holosporaceae family and two to Ca...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evx246

    authors: Wang Z,Wu M

    更新日期:2017-11-01 00:00:00

  • Evolution and Expression of Tissue Globins in Ray-Finned Fishes.

    abstract::The globin gene family encodes oxygen-binding hemeproteins conserved across the major branches of multicellular life. The origins and evolutionary histories of complete globin repertoires have been established for many vertebrates, but there remain major knowledge gaps for ray-finned fish. Therefore, we used phylogene...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evw266

    authors: Gallagher MD,Macqueen DJ

    更新日期:2017-01-01 00:00:00

  • The Anolis lizard genome: an amniote genome without isochores.

    abstract::Isochores are large regions of relatively homogeneous nucleotide composition and are present in the genomes of all mammals and birds that have been sequenced to date. The newly sequenced genome of Anolis carolinensis provides the first opportunity to quantify isochore structure in a nonavian reptile. We find Anolis to...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evr072

    authors: Fujita MK,Edwards SV,Ponting CP

    更新日期:2011-01-01 00:00:00

  • Comparative genomic analysis of malaria mosquito vector-associated novel pathogen Elizabethkingia anophelis.

    abstract::Acquisition of Elizabethkingia infections in intensive care units (ICUs) has risen in the past decade. Treatment of Elizabethkingia infections is challenging due to the lack of effective therapeutic regimens, leading to a high mortality rate. Elizabethkingia infections have long been attributed to Elizabethkingia meni...

    journal_title:Genome biology and evolution

    pub_type: 杂志文章

    doi:10.1093/gbe/evu094

    authors: Teo J,Tan SY,Liu Y,Tay M,Ding Y,Li Y,Kjelleberg S,Givskov M,Lin RT,Yang L

    更新日期:2014-05-06 00:00:00