Abstract:
:The spontaneous assembly of proteins into amyloid fibrils is a phenomenon central to many increasingly common and currently incurable human disorders, including Alzheimer's and Parkinson's diseases. Oligomeric species form transiently during this process and not only act as essential intermediates in the assembly of new filaments but also represent major pathogenic agents in these diseases. While amyloid fibrils possess a common, defining set of physicochemical features, oligomers, by contrast, appear much more diverse, and their commonalities and differences have hitherto remained largely unexplored. Here, we use the framework of chemical kinetics to investigate their dynamical properties. By fitting experimental data for several unrelated amyloidogenic systems to newly derived mechanistic models, we find that oligomers present with a remarkably wide range of kinetic and thermodynamic stabilities but that they possess two properties that are generic: they are overwhelmingly nonfibrillar, and they predominantly dissociate back to monomers rather than maturing into fibrillar species. These discoveries change our understanding of the relationship between amyloid oligomers and amyloid fibrils and have important implications for the nature of their cellular toxicity.
journal_name
Proc Natl Acad Sci U S Aauthors
Dear AJ,Michaels TCT,Meisl G,Klenerman D,Wu S,Perrett S,Linse S,Dobson CM,Knowles TPJdoi
10.1073/pnas.1922267117subject
Has Abstractpub_date
2020-06-02 00:00:00pages
12087-12094issue
22eissn
0027-8424issn
1091-6490pii
1922267117journal_volume
117pub_type
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