Abstract:
:In contrast to normal cells, cancer cells typically undergo metabolic reprogramming. Studies have shown that oncogenes play an important role in this metabolic reprogramming. CD38 is a multifunctional transmembrane protein that is expressed abnormally in a variety of tumor types. To investigate the effect and possible mechanism of CD38 in cervical cancer cells and to provide a new therapeutic target for the treatment of cervical cancer, the present study identified that CD38 is involved in regulating cell metabolism in cervical cancer cells. Liquid chromatography‑tandem mass spectrometry and bioinformatic analyses revealed that differentially abundant proteins in CD38‑overexpressed cervical cancer cells (CaSki‑CD38 and HeLa‑CD38) are predominantly involved in glycolytic pathways, oxidative phosphorylation and the NAD/NADH metabolic process. Further experiments using an ATP test kit and lactate test kit revealed that CD38 promotes glucose consumption, increases lactate accumulation and increases ATP production. In addition, CD38 increases the phosphorylation of phosphatidylserine/threonine kinase (AKT), mechanistic target of rapamycin (mTOR) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K), which play a key role in tumor metabolism. Furthermore, it was found that the energy metabolism of cervical cancer cells was inhibited following treatment with the mTOR inhibitor rapamycin. In conclusion, the results of the present study suggested that CD38 regulates the metabolism of cervical cancer cells by regulating the PI3K/AKT/mTOR pathway, which may be a candidate target for the treatment of cervical cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Liao S,Liang L,Yue C,He J,He Z,Jin X,Luo G,Zhou Ydoi
10.3892/ijo.2020.5040subject
Has Abstractpub_date
2020-07-01 00:00:00pages
338-354issue
1eissn
1019-6439issn
1791-2423journal_volume
57pub_type
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.5.893
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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doi:
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journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-08-01 00:00:00
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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doi:
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journal_title:International journal of oncology
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journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
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