Abstract:
:Pleomorphic adenomas (PAs) of salivary glands are characterized by the mixed appearance of epithelial and mesenchymal-like components such as myxoid and chondroid tissues. Although various studies have examined PAs, thus far it is not clear how PAs make these multiple components. Thus, clarification of the histodifferentiation of this unique salivary gland tumor using not only tissues in vivo but also PA cells cultured in vitro is necessary. However, no in vitro model of PA has been reported, because normal and benign tumor cells tend to grow slowly and senesce quickly in culture. Therefore, we immortalized cells using transfection of the hTERT gene without otherwise altering the nature of those cells. The immortalized PA cells expressed mRNA of the pleomorphic adenoma gene 1 and showed epithelial and neoplastic myoepithelial characteristics by immunohistochemical immunofluorescence analyses and ultrastructural study. Our findings suggest that these cells will be a useful model to study the cellular differentiation of PA.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Kitagawa M,Ogawa I,Shima K,Hashimoto S,Kudo Y,Miyauchi M,Tahara H,Shimono M,Takata Tsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
339-44issue
2eissn
1019-6439issn
1791-2423journal_volume
31pub_type
杂志文章abstract::Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway is preferentially activated in diffuse-type gastric cancer (GC) compared with intestinal-type GC; however, histological typing is currently the only method for distinguishing these two major types of GC. We compared t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2387
更新日期:2014-06-01 00:00:00
abstract::ETS variant 1 (ETV1) is an oncogenic transcription factor. However, its role in colorectal cancer has remained understudied. The present study demonstrated that ETV1 downregulation led to reduced HCT116 colorectal cancer cell growth and clonogenic activity. Furthermore, the ETV1 mRNA levels were enhanced in colorectal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5133
更新日期:2020-12-01 00:00:00
abstract::The cytosine analogue 5'-azacytidine (5'-aza) induces DNA hypomethylation by inhibiting DNA methyltransferase. In clinical trials, 5'-aza is widely used in epigenetic anticancer treatments. Accumulated evidence shows that cyclooxygenase-2 (COX-2) is overexpressed in various cancers, indicating that it may play a criti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3110
更新日期:2015-10-01 00:00:00
abstract::Peripheral blood was examined for the presence of tumor cells at multiple time points over a one-year period in 45 patients with a history of surgical resection of breast carcinoma. The number of circulating epithelial cells in 8 of 8 patients with clinically active disease concurred or preceded changes in the disease...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.573
更新日期:2000-09-01 00:00:00
abstract::New aromatase inhibitors are an exciting treatment option for postmenopausal women with hormone sensitive breast cancer. They have been shown to reduce tumors in a significant number of patients, and exhibit definite antitumor activity at a relatively low daily dose, and are highly potent, highly selective, and well-t...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.17.5.1037
更新日期:2000-11-01 00:00:00
abstract::Activation of intestinal human pregnane X receptor (PXR) has recently been proposed as a promising strategy for the chemoprevention of inflammation-induced colon cancer. The present study was aimed at evaluating the effect of rifaximin, a non-absorbable antibiotic, in inhibiting angiogenesis in a model of human colore...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3550
更新日期:2016-08-01 00:00:00
abstract::We investigated the relationship between cell growth and differentiation and COX-2 expression in oral squamous cell carcinoma (SCC) in vitro and in vivo. Treatment of SCC25 oral squamous carcinoma cells with sodium butyrate (SB) at 0.5-5 mM or all-trans retinoic acid (ATRA) at 3-300 microM inhibited cell growth and in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::Pro‑inflammatory (M1) macrophages have key roles in atherogenesis. As β‑glucan has been demonstrated to exert pro‑inflammatory effects, the present study examined whether β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype. The results from reverse transcription‑quantitative polymerase cha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4630
更新日期:2019-01-01 00:00:00
abstract::The tumor suppressor protein p53 is one of the most important manipulators of the cell cycle. By interaction with targets such as WAF1/CIP1, mdm2 or GADD45 p53 functions as a negative or positive regulator of the cell cycle. After DNA damage p53 is implicated in growth arrest of cells in the G(1) phase of the cell cyc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.1129
更新日期:1995-05-01 00:00:00
abstract::Expression and functions of interleukin (IL)-8, a pro-inflammatory cytokine with angiogenesis action, was examined in 23 surgically resected hepatocellular carcinoma (HCC) specimens and 7 HCC cell lines. In all HCC tissues, IL-8 expression was confirmed with reverse-transcription polymerase chain reaction method and e...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.2.257
更新日期:2001-02-01 00:00:00
abstract::Photodynamic therapy (PDT) following lung-sparing extended pleurectomy for malignant pleural mesothelioma (MPM) has been investigated as a potential means to kill residual microscopic cells. High expression levels of folate receptor 1 (FOLR1) have been reported in MPM; therefore, targeting FOLR1 has been considered a ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4555
更新日期:2018-11-01 00:00:00
abstract::Early detection and treatment are critical in the management of renal cell carcinoma (RCC). However, there is no standard serum biomarker to facilitate early diagnosis or prognostic stratification in patients with RCC. Recent reports suggest that circulating microRNAs (miRNAs) have great potential as biomarkers for di...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2169
更新日期:2014-01-01 00:00:00
abstract::MicroRNA‑301a (miRNA/miR‑301a) and nuclear factor (NF)‑κB signaling play important roles in tumor invasion, migration and progression. However, the role of miRNA‑301a‑3p in human gastric cancer (GC), and specifically in the activation of NF‑κB signaling, remains unclear. The aim of the present study was to investigate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5072
更新日期:2020-08-01 00:00:00
abstract::Malignant astrocytomas are highly invasive, vascular neoplasms that comprise the majority of nervous system tumors in humans. A strong association has previously been made between malignancy in human astrocytic tumors and increased expression of certain fibroblast growth factor (FGF) family members. MG-160 is an intri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Clinicopathological assessment of the functional relationship between the HER2 oncogene and tumor-associated fatty acid synthase (FASN) is largely precluded because immunohistochemical and/or mRNA studies should be performed in biopsies from breast cancer patients. We here sought to determine whether serum FASN (sFASN...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000455
更新日期:2009-12-01 00:00:00
abstract::MCA serum levels were determined in 196 patients with benign breast disease (BD) and in 371 patients with breast cancer. MCA values were abnormal in 30.6% of the patients with BD. In breast cancer patients, significant increases were only found in metastatic disease (89.1%). MCA levels were not correlated with tumor s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::The Drosophila argos protein is a natural ligand antagonist of Drosophila EGF receptor (DER), but does not interact with the human EGF receptor (EGFR). With a view to generating a corresponding EGFR antagonist, we, have designed five chimeric proteins featuring selected characteristic parts of argos within the framewo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.677
更新日期:1997-04-01 00:00:00
abstract::Chromosomal aneuploidy is associated with invasive bladder cancer and one of the genes implicated in these changes is Aurora-A/STK15/BTAK, that is localized on chromosome 20q13 and encodes a centrosome-associated serine/threonine kinase. To better understand the association between Aurora-A/STK15 expression, tumor ane...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::Primary hyperparathyroidism is a common endocrine disease with a multifaceted genetic background, the elucidation of which has only begun. Among others, loss of the short arm of chromosome 1 and somatic inactivation of the multiple endocrine neoplasia type 1 gene (MEN1) in 11q13 represent significant alterations in th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Obesity is considered a risk factor for many cancers, including breast cancer. Our laboratory has previously shown that leptin is mitogenic in many cancer cell lines, including breast. Information regarding the effects of high leptin levels on leptin receptor expression and signaling is lacking. The purpose of this st...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::Conceptually, dendritic cells (DCs) take up and process exogenous antigens that are presented on MHC (major histocompatibility complex) class II molecules to stimulate CD4+ T cells, and present endogenously-produced proteins to CD8+ T cells through an MHC class I-dependent pathway. In this study, we compared the antit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::The gp160 human kidney differentiation antigen is identical to human aminopeptidase A (APA), a zinc-dependent cell-surface metallopeptidase which hydrolyzes peptides with N-terminal acidic residues. GP160/APA is constitutively expressed by proximal tubule cells, the normal cellular counterpart of most renal cancers (R...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.2.261
更新日期:1998-08-01 00:00:00
abstract::Extracellular matrix breakdown as well as increased expression in cancer cells and tumor microvascular endothelial cells make matrix metalloproteinase 2 (MMP2) an attractive target for cancer treatment. By taking advantage of MMP2's properties, an MMP2 cleavable melittin/avidin conjugate was designed. Melittin alone i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Endometrial cancer (EC) is one of the most common malignancies of the female reproductive system, and metastasis is a major cause of mortality. In this study, we aimed to explore the role of CP‑31398 in the migration, invasion and apoptosis of EC cells by its regulation of the expression of the murine double minute 2 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4681
更新日期:2019-03-01 00:00:00
abstract::Human chondrosarcoma cells (HCS-TG) were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of human chondrosarcoma bearing an HSV-tk gene after treatment with ganciclovir. Chondrosarcoma cells bearing an HSV-tk ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.6.1137
更新日期:1999-06-01 00:00:00
abstract::Increasing evidence has confirmed that dysregulation of microRNAs (miRNAs) can contribute to the progression and metastasis of human tumors. Previous studied have shown dysregulation of miR-24 in a variety of tumors. However, the roles of miR-24 in human bladder cancer have not been well clarified. Therefore, we inves...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3117
更新日期:2015-10-01 00:00:00
abstract::VEGF, Hedgehog, FGF, Notch, and WNT signaling pathways network together for vascular remodeling during embryogenesis, tissue regeneration, and carcinogenesis. VEGFA (VEGF), VEGFB, VEGFC, VEGFD (FIGF) and PGF (PlGF) are VEGF family ligands for receptor tyrosine kinases, including VEGFR1 (FLT1), VEGFR2 (KDR) and VEGFR3 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::Neoadjuvant chemotherapy with docetaxel for advanced breast cancer can improve the radicality for a subset of patients, but some patients suffer from severe adverse drug reactions without any benefit. To establish a method for predicting responses to docetaxel, we analyzed gene expression profiles of biopsy materials ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract::Tumor metastases are the ultimate target in cancer therapy. In epithelial malignancies, the expression of high-mobility-group A2 (HMGA2) is associated with disease progression and the epithelial-mesenchymal transition (EMT), which is involved in the metastatic process. The present study assessed the clinical and molec...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2947
更新日期:2015-01-01 00:00:00
abstract::Insulin-like growth factor-binding protein-2 (IGFBP-2) is considered to be a human tumor antigen, and the tumor-specific immunity of IGFBP-2 has been reported in several types of cancer. The purpose of this study was to evaluate whether autoantibodies to IGFBP-2 can be used as diagnostic markers in lung cancer. The re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1699
更新日期:2013-01-01 00:00:00