Abstract：
:Aberrant Notch signaling plays a pivotal role in T-cell acute lymphoblastic leukemia (T-ALL) and chronic lymphocytic leukemia (CLL). Amplitude and duration of the Notch response is controlled by ubiquitin-dependent proteasomal degradation of the Notch1 intracellular domain (NICD1), a hallmark of the leukemogenic process. Here, we show that HDAC3 controls NICD1 acetylation levels directly affecting NICD1 protein stability. Either genetic loss-of-function of HDAC3 or nanomolar concentrations of HDAC inhibitor apicidin lead to downregulation of Notch target genes accompanied by a local reduction of histone acetylation. Importantly, an HDAC3-insensitive NICD1 mutant is more stable but biologically less active. Collectively, these data show a new HDAC3- and acetylation-dependent mechanism that may be exploited to treat Notch1-dependent leukemias.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ferrante F,Giaimo BD,Bartkuhn M,Zimmermann T,Close V,Mertens D,Nist A,Stiewe T,Meier-Soelch J,Kracht M,Just S,Klöble P,Oswald F,Borggrefe Tdoi
10.1093/nar/gkaa088subject
Has Abstractpub_date
2020-04-17 00:00:00pages
3496-3512issue
7eissn
0305-1048issn
1362-4962pii
5763097journal_volume
48pub_type
杂志文章abstract：:Phosphoprotein-binding domains (PPBDs) mediate many important cellular and molecular processes. Ten PPBDs have been known to exist in the human proteome, namely, 14-3-3, BRCT, C2, FHA, MH2, PBD, PTB, SH2, WD-40 and WW. PepCyber:P approximately PEP is a newly constructed database specialized in documenting human PPBD-c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm854
更新日期：2008-01-01 00:00:00
abstract：:The IMGT/HLA database (http://www.ebi.ac.uk/imgt/hla) has provided a centralized repository for the sequences of the alleles named by the WHO Nomenclature Committee for Factors of the HLA System for the past four years. Since its initial release the database has grown and is the primary source of information for the s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg070
更新日期：2003-01-01 00:00:00
abstract：:This paper describes the sequence of 257 nucleotides from the 3' end of RNA 2 of barley stripe mosaic virus ( BSMV , strain Argentina Mild) including an internal oligo (A) tract localized at a distance of 236 nucleotides from the 3' end, and the 3' terminal tRNA-like structure accepting tyrosine. This sequence is show...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.4001
更新日期：1984-05-11 00:00:00
abstract：:The hepatocyte nuclear factor (HNF)4alpha, a member of the nuclear receptor superfamily, regulates genes that play a critical role in embryogenesis and metabolism. Recent studies have shown that mutations in the human HNF4alpha gene cause a rare form of type 2 diabetes, maturity onset diabetes of the young (MODY1). To...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.2.430
更新日期：2000-01-15 00:00:00
abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR), in combination with CRISPR associated (cas) genes, constitute CRISPR-Cas bacterial adaptive immune systems. To generate immunity, these systems acquire short sequences of nucleic acids from foreign invaders and incorporate these into their CRISPR arra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku527
更新日期：2014-07-01 00:00:00
abstract：:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.22.5507
更新日期：1980-11-25 00:00:00
abstract：:We have developed a new COFACTOR webserver for automated structure-based protein function annotation. Starting from a structural model, given by either experimental determination or computational modeling, COFACTOR first identifies template proteins of similar folds and functional sites by threading the target structu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks372
更新日期：2012-07-01 00:00:00
abstract：:A 12 bp long GCN4-binding, self-complementary duplex DNA d(CATGACGTCATG)(2) has been investigated by NMR spectroscopy to study the structure and dynamics of the molecule in aqueous solution. The NMR structure of the DNA obtained using simulated annealing and iterative relaxation matrix calculations compares quite clos...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.2.499
更新日期：2001-01-15 00:00:00
abstract：:Modular primers are strings of three contiguously annealed unligated oligonucleotides (modules) as short as 5- or 6-mers, selected from a presynthesized library. It was previously found that such strings can prime DNA sequencing reactions specifically, thus eliminating the need for the primer synthesis step in DNA seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.2881
更新日期：1995-08-11 00:00:00
abstract：:Four hundred twenty-eight high-resolution DNA-protein complexes were chosen for a bioinformatics study. Although 164 crystal structures (38% of those searched) contained no interactions, 574 discrete π-contacts between the aromatic amino acids and the DNA nucleobases or deoxyribose were identified using strict criteri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku269
更新日期：2014-06-01 00:00:00
abstract：:The complete sequences of three kinetoplast DNA minicircles (B4, D3 and D12) from Leishmania tarentolae are reported. All L. tarentolae minicircles encode single gRNAs localized within the variable region approximately 150 bp from the conserved region. The 5' termini and tentative 3' termini of the new gRNAs were dete...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.22.6277
更新日期：1991-11-25 00:00:00
abstract：:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.1.19
更新日期：1989-01-11 00:00:00
abstract：:Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz231
更新日期：2019-06-04 00:00:00
abstract：:Glucocorticoid (GC) signaling synchronizes the circadian rhythm of individual peripheral cells and induces the expression of circadian genes, including Period1 (Per1) and Period2 (Per2). However, no GC response element (GRE) has been reported in the Per2 promoter region. Here we report the molecular mechanisms of Per2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt307
更新日期：2013-07-01 00:00:00
abstract：:The EMBL Data Library was the first internationally supported central resource for nucleic acid sequence data. Working in close collaboration with its American counterpart, GenBank (1), the library prepares and makes available to the scientific community a comprehensive collection of the published nucleic acid sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.1.5
更新日期：1986-01-10 00:00:00
abstract：:Single-stranded mRNA molecules form secondary structures through complementary self-interactions. Several hypotheses have been proposed on the relationship between the nucleotide sequence, encoded amino acid sequence and mRNA secondary structure. We performed the first transcriptome-wide in silico analysis of the huma...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl287
更新日期：2006-05-08 00:00:00
abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp554
更新日期：2009-09-01 00:00:00
abstract：:CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expresse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq335
更新日期：2010-09-01 00:00:00
abstract：:Treatment of cells with DNA damaging agents leads to induction of a variety of genes involved in different cellular processes. We have applied a lacZ-based gene trap strategy to search for new mammalian genes induced by genotoxic stress. A population of 32 x 10(3) neo r clones stably transfected with a gene trap vecto...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.23.4803
更新日期：1997-12-01 00:00:00
abstract：:The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the lef...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt233
更新日期：2013-05-01 00:00:00
abstract：:The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length. Recently, additional cDNA sequences have been reported that lie upstream of the original and contain an extended open reading frame with three additional ATGs in frame with the coding region [Tucker et al . (1996) Pro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.22.4666
更新日期：1997-11-15 00:00:00
abstract：:2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.10.2421
更新日期：1992-05-25 00:00:00
abstract：:The cytosine base in DNA undergoes hydrolytic deamination at a considerable rate when UV radiation induces formation of a cyclobutane pyrimidine dimer (CPD) with an adjacent pyrimidine base. We have synthesized a phosphoramidite building block of a cis-syn cyclobutane thymine-uracil dimer (T[]U), which is the deaminat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh342
更新日期：2004-03-12 00:00:00
abstract：:Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1-ORF2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl531
更新日期：2006-01-01 00:00:00
abstract：:Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiRe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx610
更新日期：2017-09-06 00:00:00
abstract：:Previously we identified Rrp1 and Rrp2 as two proteins required for the Sfr1/Swi5-dependent branch of homologous recombination (HR) in Schizosaccharomyces pombe. Here we use a yeast two-hybrid approach to demonstrate that Rrp1 and Rrp2 can interact with each other and with Swi5, an HR mediator protein. Rrp1 and Rrp2 f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt564
更新日期：2013-09-01 00:00:00
abstract：:The pac1+ gene of the fission yeast Schizosaccharomyces pombe is essential for viability and its overexpression induces sterility and suppresses mutations in the pat1+ and snm1+ genes. The pac1+ gene encodes a protein that is structurally similar to RNase III from Escherichia coli, but its normal function is unknown. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.12.2377
更新日期：1996-06-15 00:00:00
abstract：:Furvina®, also denominated G1 (MW 297), is a synthetic nitrovinylfuran [2-bromo-5-(2-bromo-2-nitrovinyl)-furan] antibiotic with a broad antimicrobial spectrum. An ointment (Dermofural®) containing G1 as the only active principle is currently marketed in Cuba and successfully used to treat dermatological infections. He...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks822
更新日期：2012-11-01 00:00:00
abstract：:RNA molecules play different roles in coding, decoding and gene expression regulation. Such roles are often associated to the RNA secondary or tertiary structures. The folding dynamics lead to multiple secondary structures of long RNA molecules, since an RNA molecule might fold into multiple distinct native states. De...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1249
更新日期：2014-12-16 00:00:00
abstract：:Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq801
更新日期：2011-01-01 00:00:00
