Abstract:
:The endangered medicinal plant Glehnia littoralis is one of the important natural source of furanocoumarin, which has been used as mucolytic, antitussive, antitumour and antibacterial. However, the genetic information of furanocoumarin biosynthesis in G. littoralis is scarce at present. The objective of this study was to mine the putative candidate genes involved in the biosynthesis pathwayof furanocoumarin and provide references for gene identification, and functional genomics of G. littoralis. We carried out the transcriptome analysis of leaves and roots in G. littoralis, which provided a dataset for gene mining. Psoralen, imperatorin and isoimperatorin were detected in G. littoralis by high performance liquid chromatography analysis. Candidate key genes were mined based on the annotations and local BLAST with homologous sequences using BioEdit software. The relative expression of genes was analysed using quantitative real-time polymerase chain reaction. Further, the CYP450 genes were mined using phylogenetic analyses using MEGA 6.0 software. Atotal of 156,949 unigenes were generated, of which 9021 were differentially-expressed between leaves and roots. A total of 82 unigenes encoding eight enzymes in furanocoumarin biosynthetic pathway were first obtained. Seven genes that encoded key enzymes in the downstream furanocoumarin biosynthetic pathway and expressed more in roots than leaves were screened. Twenty-six candidate CYP450 unigenes expressed abundantly in roots and were chiefly concentrated in CYP71, CYP85 and CYP72 clans. Finally, we filtered 102 differentially expressed transcription factors (TFs) unigenes. The transcriptome of G. littoralis was characterized which would help to elucidate the furanocoumarin biosynthetic pathway in G. littoralis and provide an invaluable resource for further study of furanocoumarin.
journal_name
J Genetjournal_title
Journal of geneticsauthors
Song J,Luo H,Xu Z,Zhang Y,Xin H,Zhu D,Zhu X,Liu M,Wang W,Ren H,Chen H,Gao Tsubject
Has Abstractpub_date
2020-01-01 00:00:00eissn
0022-1333issn
0973-7731journal_volume
99pub_type
杂志文章abstract::Garcinia gummi-gutta (L.) Roxb. (Clusiaceae) is an endemic, semidomesticated, fruit-yielding tree species distributed in the Western Ghats of India and Sri Lanka. Various bioactive phytochemicals, such as garcinol, benzophenones and xanthones are isolated from G. gummi-gutta and have shown antibacterial, antiviral and...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-017-0756-0
更新日期:2017-06-01 00:00:00
abstract::Allohexaploid wheat was derived from interspecific hybridization, followed by spontaneous chromosome doubling. Newly synthesized hexaploid wheat by crossing Triticum turgidum and Aegilops tauschii provides a classical model to understand the mechanisms of allohexaploidization in wheat. However, immediate chromosome le...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-016-0704-4
更新日期:2016-12-01 00:00:00
abstract::Nonrandom segregation ratios of alleles 'segregation distortion' can have a striking impact on transmission genetics, and with widespread availability of genetic markers has been shown to be a frequent phenomenon. To investigate the possible effect of genetic interaction on segregation distortion and genetic map const...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2018-12-01 00:00:00
abstract::Metabolic syndrome (MetS) is an inflammatory disorder, in which various cytokines play important role in tilting balance towards disease state. Interleukin-10 (IL-10) is an important antiinflammatory cytokine, but its genetic polymorphisms and serum levels in Indian MetS subjects are unknown. Three IL-10 gene polymorp...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-016-0738-7
更新日期:2017-03-01 00:00:00
abstract::Male fertility in farm animals is considered as an important economic trait. The phenomenon of spermatogenesis plays a dynamic functional role in determining the viability of sperm and thereby can impact on fertility-driven complications. The process of spermatogenesis is controlled by numerous molecular factors and r...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract::Genomewide association study (GWAS) is an efficient tool for the detection of SNPs and candidate genes in quantitative traits. Growth rate is an important trait for increasing the meat production in sheep. A total of 96 Baluchi sheep were genotyped using Illumina Ovine SNP50 BeadChip to run a GWAS for an average daily...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2018-06-01 00:00:00
abstract::miRNAs are important regulators of plant gene expression. There are few studies on the regulation of miRNAs in Lonicera edulis. We used high-throughput sequencing technology to analyse miRNAs in L. edulis, aiming to identify miRNAs and elucidate their function in L. edulis. In the present study, we employed the high-t...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract::The evolutionary dynamics of the tetra-nucleotide microsatellite locus Spl-106 were investigated at the repeat and flanking sequences in 137 individuals of 15 Acipenseriform species, giving 93 homologous sequences, which were detected in 11 out of 15 species. Twenty-three haplotypes of flanking sequences and three dis...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-011-0055-0
更新日期:2011-08-01 00:00:00
abstract::We have analysed the evolution of ERG28/C14orf1, a gene coding for a protein involved in sterol biosynthesis. While primary sequence of the protein is well conserved in all organisms able to synthesize sterols de novo, strong divergence is noticed in insects, which are cholesterol auxotrophs. In spite of this virtual ...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/BF02811414
更新日期:2001-04-01 00:00:00
abstract::Long noncoding RNA (lncRNA) H19, a well-known oncogenic lncRNA, is overexpressed in various cancers. Several studies have investigated the association between polymorphisms in lncRNA H19 and the risk of various cancer types; however, the findings were inconsistent. In this study, we performed a meta-analysis to identi...
journal_title:Journal of genetics
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2019-09-01 00:00:00
abstract::To understand the phylogenetic position of Bostrychus sinensis in Eleotridae and the phylogenetic relationships of the family, we determined the nucleotide sequence of the mitochondrial (mt) genome of Bostrychus sinensis. It is the first complete mitochondrial genome sequence of Bostrychus genus. The entire mtDNA sequ...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-013-0259-6
更新日期:2013-01-01 00:00:00
abstract::Negative supercoiling stimulates transcription of many genes. In contrast, transcription of the genes coding for DNA gyrase is subject to a novel mechanism of autoregulation, wherein relaxation of the template DNA stimulates their transcription. Since DNA gyrase is the sole supercoiling activity in the eubacterial cel...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/BF02717907
更新日期:2001-12-01 00:00:00
abstract::Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple bZIPs have been identified and investigated in numerous plant species. However, few studies have characterized...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract::The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of t...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-014-0351-6
更新日期:2014-04-01 00:00:00
abstract::Genetic correlations of nutrient quality traits including lysine, methionine, leucine, isoleucine, phenylalanine, valine and threonine contents in rapeseed meal were analysed by the genetic model for quantitative traits of diploid plants using a diallel design with nine parents of Brassica napus L. These results indic...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-011-0040-7
更新日期:2011-04-01 00:00:00
abstract::Genotypes of 103 short tandem repeat (STR) markers distributed at an average of 40 cM intervals throughout the genome were determined for 40 individuals from the village of BirEl Hfai (BEH). This village of approximately 31,000 individuals is localized in the south-west of Tunisia. The allele frequency distributions i...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/BF02715830
更新日期:2004-04-01 00:00:00
abstract::While much of our understanding of genetic inheritance is based on the genome of the organism, it is becoming clear that there is an ample amount of epigenetic inheritance, which though reversible, escapes erasing process during gametogenesis and goes on to the next generation. Several examples of transgenerational in...
journal_title:Journal of genetics
pub_type: 杂志文章,评审
doi:10.1007/s12041-017-0788-5
更新日期:2017-07-01 00:00:00
abstract::Gene coexpression patterns can reveal gene collections with functional consistency. This study systematically constructs regulatory networks for pituitary tumours by integrating gene coexpression, transcriptional and posttranscriptional regulation. Through network analysis, we elaborate the incidence mechanism of pitu...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-013-0299-y
更新日期:2013-12-01 00:00:00
abstract::Equal transmission of the two alleles at a locus from a heterozygote parent to the offspring is rarely violated. Beside the differential embryonic mortality, nondisjunction and gene conversion that are rather irregular forms of transmission-ratio distortion (TRD), there are two major forms of departure from Mendelian ...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-008-0019-1
更新日期:2008-08-01 00:00:00
abstract::Sorghum downy mildew caused by Peronosclerospora sorghi is a major disease of maize and resistance is under the control of polygenes which necessitated identification of quantitative-trait loci (QTLs) for initiating marker-assisted introgression of resistant QTLs in elite susceptible inbred lines. In the present study...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-015-0590-1
更新日期:2015-12-01 00:00:00
abstract::The Tetraodontidae (pufferfishes), is primarily a family of marine and estuarine fishes with a limited number of freshwater species. Freshwater invasions can be observed in South America, Southeast Asia and central Africa. In the present study, we have analysed the complete mitogenome of freshwater pufferfish, Carinot...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2019-12-01 00:00:00
abstract::Eucalyptus is an important short rotation pulpy woody plant, grown widely in the tropics. Recently, many genomic programmes are underway leading to the accumulation of voluminous genomic and expressed sequence tag sequences in public databases. These sequences can be utilized for analysis of simple sequence repeats (S...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-008-0003-9
更新日期:2008-04-01 00:00:00
abstract::Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, the her6 gene, a member of the HES family, is the homologue of hes1 in mammals and chicken. In this study, the her6 gene and its...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/BF02935327
更新日期:2006-12-01 00:00:00
abstract::IQSEC2 is an X-linked gene highly expressed at the excitatory synapses where it plays a crucial role in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking and synaptic plasticity. To date, several males and females with severe to profound intellectual disability have been reported harbouring fra...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract::Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants.In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class G...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-015-0538-5
更新日期:2015-09-01 00:00:00
abstract::Chronic periodontitis (CP) is the common form of inflammatory oral disease. Matrix metalloproteinases (MMPs) play a pivotal role in the progression of CP by degrading gingival tissue and its remodelling. Here, we conducted a case-control study to investigate a possible association of single-nucleotide polymorphism of ...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2019-03-01 00:00:00
abstract::Proline and glutamine-rich wheat seed endosperm proteins are collectively referred to as prolamins. They are comprised of HMW-GSs, LMW-GSs and gliadins. HMW-GSs are major determinants of gluten elasticity and LMW-GSs considerably affect dough extensibility and maximum dough resistance. The inheritance of glutenin subu...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-010-0025-y
更新日期:2010-08-01 00:00:00
abstract::Insulin-like growth factor receptor (IGF-1R) deficiency is a rare form of short stature, and is difficult to clinically diagnose. Targeted next-generation sequencing (NGS) allows for the rapid and inexpensive assessment of short stature. We identified mutations in the pedigree of a Chinese boy with severe short statur...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2019-03-01 00:00:00
abstract::The present study aimed to identify the alternatively spliced isoforms of pig MEF2A gene and to determine theirmRNA expression patterns. Four alternatively spliced isoforms of pig MEF2A gene (i.e. MEF2A1, MEF2A2, MEF2A3 and MEF2A4) were cloned according to the results of transcriptome sequencing. The fifth to eighth e...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:
更新日期:2018-09-01 00:00:00
abstract::Environmental stress has been suggested to be a major evolutionary force, both through inducing strong selection and because of its direct impact on developmental buffering processes that alter the evolvability of organisms. In particular, temperature has attracted much attention because of its importance as an ecolog...
journal_title:Journal of genetics
pub_type: 杂志文章
doi:10.1007/s12041-008-0063-x
更新日期:2008-12-01 00:00:00