Abstract:
:This study investigated the contribution of small leucine rich proteoglycans (SLRPs) to the fracture toughness of the dentin extracellular matrix (ECM) by enzymatically-assisted selective removal of glycosaminoglycan chains (GAGs) and proteoglycans (PGs) core protein. We adapted the Mode III trouser tear test to evaluate the energy required to tear the dentin ECM. Trouser-shaped dentin specimens from crown and root were demineralized. Depletion of GAGs and PGs followed enzymatic digestion using chondroitinase ABC (c-ABC) and matrix metalloproteinase 3 (MMP-3), respectively. The legs from specimen were stretched under tensile force and the load at tear propagation was determined to calculate the tear energy (T, kJ/m2). SLRPs decorin and biglycan were visualized by immunohistochemistry and ECM tear pattern was analyzed in SEM. Results showed T of crown ECM was not affected by PGs/GAGs depletion (p = 0.799), whereas the removal of PGs significantly reduced T in root dentin ECM (p = 0.001). Root dentin ECM exhibited higher T than crown (p < 0.03), however no regional difference are present after PG depletion (p = 0.480). Immunohistochemistry confirmed removal of GAGs and PGs. SEM images showed structural modifications after PGs/GAGs removal such as enlargement of dentinal tubules, increased interfibrillar spaces and presence of untwisted fibrils with increased diameter. Findings indicate that the capacity of the PGs to unfold and untwist contribute to the dentin ECM resistance to tear, possibly influencing crack growth propagation. The regional differences are likely an evolutionary design to increase tooth survival, that undergoes repetitive mechanical loading and load stress dissipation over a lifetime of an individual.
journal_name
J Biomechjournal_title
Journal of biomechanicsauthors
Alania Y,Creighton J,Trevelin LT,Zamperini CA,Bedran-Russo AKdoi
10.1016/j.jbiomech.2020.109633subject
Has Abstractpub_date
2020-03-05 00:00:00pages
109633eissn
0021-9290issn
1873-2380pii
S0021-9290(20)30040-3journal_volume
101pub_type
杂志文章abstract::This study investigated the plantar pressure distribution during gait on wooden surface with different slipperiness in the presence of contaminants. Fifteen Chinese males performed 10 walking trials on a 5-m wooden walkway wearing cloth shoe in four contaminated conditions (dry, sand, water, oil). A pressure insole sy...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2007.11.001
更新日期:2008-01-01 00:00:00
abstract::It has long been thought that intracranial saccular aneurysms enlarge and rupture because of mechanical instabilities. Recent nonlinear analyses suggest, however, that at least certain sub-classes of aneurysms do not exhibit quasi-static limit point instabilities or dynamic instabilities in response to periodic loadin...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/s0021-9290(03)00083-6
更新日期:2003-08-01 00:00:00
abstract::Micro-Finite Element (micro-FE) analysis is now widely used in biomedical research as a tool to derive bone mechanical properties as they relate to its microstructure. With the development of in vivo high-resolution peripheral quantitative CT (HR-pQCT) scanners, it can now be applied to analyze bone in-vivo in the per...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2014.12.024
更新日期:2015-03-18 00:00:00
abstract::Poisson's ratio has not been experimentally measured earlier for meniscus in compression. It is however an important intrinsic material property needed in biomechanical analysis and computational models. In this study, equilibrium Poisson's ratio of bovine meniscus (n = 6) was determined experimentally by combining st...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2018.07.001
更新日期:2018-08-22 00:00:00
abstract::Interstitial fluid pressurization, a consequence of a biphasic tissue structure, is essential to the load bearing and lubrication properties of articular cartilage. Focal tissue degradation may interfere with this protective mechanism, eventually leading to gross degeneration and osteoarthritis. Our long-term goal is ...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2011.12.019
更新日期:2012-04-05 00:00:00
abstract::In a newly developed, highly sensitive dilatometer we applied pulsatile electric fields to five dry bone samples cut from mid-tibial sections within a 90 degrees angle from the rear to front axis. Samples of five cows were studied. We measured the piezoelectric coefficient d23 establishing its mean and confidence inte...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/s0021-9290(99)00021-4
更新日期:1999-06-01 00:00:00
abstract::The estimation of segment inertial parameters (SIPs) is an important source of error in inverse dynamic analysis. In most individual cases SIPs are derived from extrapolation of known SIPs of a certain population through regression equations (proportional models). Another well-known method is the use of mathematical a...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/0021-9290(95)00086-0
更新日期:1996-05-01 00:00:00
abstract::Risk for injury during a fall depends on the position and velocity of the body segments at the moment of impact. One technique for reducing impact velocity is to absorb energy in the lower extremity muscles during descent, as occurs during squatting or sitting. However, the protective value of this response may depend...
journal_title:Journal of biomechanics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jbiomech.2003.12.015
更新日期:2004-09-01 00:00:00
abstract::The majority of fall-related accidents are during stair ambulation-occurring commonly at the top and bottom stairs of each flight, locations in which individuals are transitioning to stairs. Little is known about how individuals adjust their biomechanics in anticipation of walking-stair transitions. We identified the ...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2015.12.041
更新日期:2016-02-29 00:00:00
abstract::Mechnotransduction, the phenomenon by which cells respond to applied force, is necessary for normal cell processes and is implicated in the pathology of several diseases including atherosclerosis. The exact mechanisms which govern how forces can affect gene expression have not been determined, but putative direct forc...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2008.08.024
更新日期:2008-11-14 00:00:00
abstract::The effect of screw-bone interface modelling strategies was evaluated in the setting of a tibial mid-shaft fracture stabilised using locking plates. Three interface models were examined: fully bonded interface; screw with sliding contact with bone; and screw with sliding contact with bone in an undersized pilot hole. ...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2012.04.008
更新日期:2012-06-01 00:00:00
abstract::Stress relaxation experiments were performed on specimens from a human intervertebral disc. Specimens were made from the nucleus pulposus and from the external lamellae of the anulus fibrosus in two different orientations. Tests were run with varying moisture content so as to develop a relaxation master curve. A model...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/0021-9290(87)90144-8
更新日期:1987-01-01 00:00:00
abstract::The role of microcracking in cortical bone as a toughening mechanism has been investigated in conjunction with the variation in fracture toughness with crack length. Fracture toughness tests were conducted on miniaturised compact tension specimens made from human and bovine cortical bone and the resultant microstructu...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/s0021-9290(97)00029-8
更新日期:1997-08-01 00:00:00
abstract::This study was aimed at investigating the intersegmental coordination of six older adults while managing unexpected slippages delivered during steady walking, and wearing an Active Pelvis Orthosis (APO). The APO was setup either to assist volunteers at the hip levels during balance loss or to be transparent. The Plana...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2019.01.003
更新日期:2019-03-06 00:00:00
abstract::This study suggests a method that, on the basis of concurrent cinematographic and dynamometric measurements, detects the motion of the visceral part of the body, provided that an estimate of its mass has been given in advance and the periodicity of the system dynamics can be assumed. The mathematical model provides eq...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/0021-9290(94)90036-1
更新日期:1994-01-01 00:00:00
abstract::Relatively high rates of loosening and implant failure have been reported after total ankle arthroplasty. Abnormal kinematics and incongruency of the articular surface may cause increased contact pressure and rotational torque applied to the implant, leading to loosening and implant failure. We measured in vivo kinema...
journal_title:Journal of biomechanics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jbiomech.2012.05.043
更新日期:2012-08-09 00:00:00
abstract::The three-dimensional trajectory of the body's centre of mass (COM) is useful to determine a number of biomechanical outcomes in running research. Previous studies have used the COM to calculate measures such as overstriding, vertical stiffness, and vertical oscillation. The COM is traditionally computed using the seg...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2020.109886
更新日期:2020-07-17 00:00:00
abstract::The accumulation of a fluid property from the standpoint of a particle moving with non-steady fluid flow (i.e., platelet/blood-cell damage index in pulsating blood flow) is a challenged computational problem due to the current need for particle-tracing methods. The method we developed (dubbed VPI) enables the approxim...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2009.11.011
更新日期:2010-03-22 00:00:00
abstract::A conspicuous long-term consequence of hypertension is a thickening of the arterial wall, which many suggest returns the circumferential wall stress toward its normal value. This thickening results from an increase in smooth muscle and extracellular matrix, with the associated growth and remodeling processes depending...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/s0021-9290(03)00178-7
更新日期:2003-11-01 00:00:00
abstract::Despite significant evidence regarding the increased risk of cartilage degeneration due to traumatic injuries to joints, there is still a lack of understanding of the mechanisms underlying osteoarthritis development following a joint injury. Injuries in knee cartilage are often characterized by lesions or tears. In ad...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2020.109970
更新日期:2020-09-18 00:00:00
abstract::Leakage of blood alongside the implant is a relatively frequent and life-limiting complication after transcatheter aortic valve implantation. The aim of this study is to develop and validate a workflow to simulate the implantation prior to the intervention. Based on the simulation outcome, the amount of leakage is est...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2016.06.024
更新日期:2016-09-06 00:00:00
abstract::Aging brings about challenges in the ability to recover balance through protective stepping, especially in the lateral direction. Previous work has suggested that lateral protective stepping during weight transfer may be affected by impaired muscle composition and performance of the hip abductors (AB) in older adults....
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2018.10.040
更新日期:2019-01-03 00:00:00
abstract::The present article investigates the influence of center-of-mass movements on the variation of the structure in human postural sway. Twelve healthy younger persons performed 60s quiet standing, 60s relaxed standing, and 10 min relaxed standing on two force plates. Center-of-pressure (CoP) and gravitational line (GL) p...
journal_title:Journal of biomechanics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jbiomech.2012.10.016
更新日期:2013-02-01 00:00:00
abstract::Recent studies raised increasing concern about the reliability of computer models in reproducing in vivo hemodynamics in cerebral aneurysms. Boundary condition problem is among the most frequently addressed issues since three-dimensional (3-D) modeling is usually restricted to local arterial segments. The present stud...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2016.09.031
更新日期:2016-11-07 00:00:00
abstract::Body segment parameters (BSP) are essential input for the computations in kinetics of motion applied in the field of biomechanics. These data are usually obtained from population-specific predictive equations which present limitations in being representative of the population under study. More recently, medical imagin...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2008.10.036
更新日期:2009-02-09 00:00:00
abstract::Simple models are widely used to understand the mechanics of human walking. The optimization-based minimal biped model and spring-loaded-inverted-pendulum (SLIP) model are two popular models that can achieve human-like walking patterns. However, ground reaction forces (GRF) from these two models still deviate from exp...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2019.04.028
更新日期:2019-06-11 00:00:00
abstract::How small do pressure transducers need to be in order to faithfully measure the plantar pressure profiles (PPPs) under normal and diabetic feet? In this study, pressures were collected from five diabetic and six non-diabetic subjects using a commercial measurement system with 25 mm2 transducers. Discrete Fourier Trans...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/0021-9290(95)00116-6
更新日期:1996-07-01 00:00:00
abstract::Healthy patellofemoral (PF) joint mechanics are critical to optimal function of the knee joint. Patellar maltracking may lead to large joint reaction loads and high stresses on the articular cartilage, increasing the risk of cartilage wear and the onset of osteoarthritis. While the mechanical sources of PF joint dysfu...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2015.12.020
更新日期:2016-01-25 00:00:00
abstract::The patchy distribution of atherosclerosis within arteries is widely attributed to local variation in haemodynamic wall shear stress (WSS). A recently-introduced metric, the transverse wall shear stress (transWSS), which is the average over the cardiac cycle of WSS components perpendicular to the temporal mean WSS vec...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2016.11.035
更新日期:2017-01-04 00:00:00
abstract::Wheelchair-dependent subjects often adopt a passive sitting posture and suffer from sitting acquired pressure ulcers (PU) that mainly occur when high buttock pressures sustain for a longer period of time. Body posture directly influences seating load and proper postural change is therefore essential. Many subjects can...
journal_title:Journal of biomechanics
pub_type: 杂志文章
doi:10.1016/j.jbiomech.2008.04.012
更新日期:2008-07-19 00:00:00