Regional contribution of proteoglycans to the fracture toughness of the dentin extracellular matrix.

Abstract:

:This study investigated the contribution of small leucine rich proteoglycans (SLRPs) to the fracture toughness of the dentin extracellular matrix (ECM) by enzymatically-assisted selective removal of glycosaminoglycan chains (GAGs) and proteoglycans (PGs) core protein. We adapted the Mode III trouser tear test to evaluate the energy required to tear the dentin ECM. Trouser-shaped dentin specimens from crown and root were demineralized. Depletion of GAGs and PGs followed enzymatic digestion using chondroitinase ABC (c-ABC) and matrix metalloproteinase 3 (MMP-3), respectively. The legs from specimen were stretched under tensile force and the load at tear propagation was determined to calculate the tear energy (T, kJ/m2). SLRPs decorin and biglycan were visualized by immunohistochemistry and ECM tear pattern was analyzed in SEM. Results showed T of crown ECM was not affected by PGs/GAGs depletion (p = 0.799), whereas the removal of PGs significantly reduced T in root dentin ECM (p = 0.001). Root dentin ECM exhibited higher T than crown (p < 0.03), however no regional difference are present after PG depletion (p = 0.480). Immunohistochemistry confirmed removal of GAGs and PGs. SEM images showed structural modifications after PGs/GAGs removal such as enlargement of dentinal tubules, increased interfibrillar spaces and presence of untwisted fibrils with increased diameter. Findings indicate that the capacity of the PGs to unfold and untwist contribute to the dentin ECM resistance to tear, possibly influencing crack growth propagation. The regional differences are likely an evolutionary design to increase tooth survival, that undergoes repetitive mechanical loading and load stress dissipation over a lifetime of an individual.

journal_name

J Biomech

journal_title

Journal of biomechanics

authors

Alania Y,Creighton J,Trevelin LT,Zamperini CA,Bedran-Russo AK

doi

10.1016/j.jbiomech.2020.109633

subject

Has Abstract

pub_date

2020-03-05 00:00:00

pages

109633

eissn

0021-9290

issn

1873-2380

pii

S0021-9290(20)30040-3

journal_volume

101

pub_type

杂志文章
  • T2 relaxation time measurements in tibiotalar cartilage after barefoot running and its relationship to ankle biomechanics.

    abstract::The influence of ankle kinematics and plantar pressure from mid-range barefoot running on T2 relaxation times of tibiotalar cartilage is unknown. This study aimed to quantitatively evaluate the T2 relaxation time of tibiotalar cartilage and ankle biomechanics following 5 km barefoot running. Twenty healthy runners (wh...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2019.04.046

    authors: Kyung Kim H,Fernandez J,Logan C,Tarr GP,Doyle A,Mirjalili SA

    更新日期:2019-06-11 00:00:00

  • Alteration of blood hemorheologic properties during cerebral ischemia and reperfusion in rats.

    abstract::The cerebral ischemia and reperfusion rat model was employed in this experiment to study the rheological properties (i.e. viscosity, hematocrit, red blood cell deformability and thixotropic properties) of whole blood. The results of this study show that a significant relation exists between the duration of cerebral is...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/s0021-9290(00)00180-9

    authors: Kuke D,Donghua L,Xiaoyan S,Yanjun Z

    更新日期:2001-02-01 00:00:00

  • Expedited patient-specific assessment of contact stress exposure in the ankle joint following definitive articular fracture reduction.

    abstract::Acute injury severity, altered joint kinematics, and joint incongruity are three important mechanical factors linked to post-traumatic osteoarthritis (PTOA). Finite element analysis (FEA) was previously used to assess the influence of increased contact stress due to joint incongruity on PTOA development. While promisi...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2015.05.030

    authors: Kern AM,Anderson DD

    更新日期:2015-09-18 00:00:00

  • Anatomic variation in the elastic inhomogeneity and anisotropy of human femoral cortical bone tissue is consistent across multiple donors.

    abstract::Numerical models commonly account for elastic inhomogeneity in cortical bone using power-law scaling relationships with various measures of tissue density, but limited experimental data exists for anatomic variation in elastic anisotropy. A recent study revealed anatomic variation in the magnitude and anisotropy of el...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2011.04.009

    authors: Rudy DJ,Deuerling JM,Espinoza Orías AA,Roeder RK

    更新日期:2011-06-03 00:00:00

  • Fluid flow in bone in vitro.

    abstract::Recent dielectric measurements suggest that the electromechanical effect in wet (fluid saturated) bone is not due to a piezoelectric effect. This would imply that the electromechanical effect observed in wet bone is due to a streaming potential and is therefore dependent on fluid flow in stressed bone. A model for flu...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/0021-9290(82)90054-9

    authors: Johnson MW,Chakkalakal DA,Harper RA,Katz JL,Rouhana SW

    更新日期:1982-01-01 00:00:00

  • Evaluation of an aortic valve prosthesis: Fluid-structure interaction or structural simulation?

    abstract::Bio-inspired polymeric heart valves (PHVs) are excellent candidates to mimic the structural and the fluid dynamic features of the native valve. PHVs can be implanted as prosthetic alternative to currently clinically used mechanical and biological valves or as potential candidate for a minimally invasive treatment, lik...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2017.04.004

    authors: Luraghi G,Wu W,De Gaetano F,Rodriguez Matas JF,Moggridge GD,Serrani M,Stasiak J,Costantino ML,Migliavacca F

    更新日期:2017-06-14 00:00:00

  • Stress deprivation simultaneously induces over-expression of interleukin-1beta, tumor necrosis factor-alpha, and transforming growth factor-beta in fibroblasts and mechanical deterioration of the tissue in the patellar tendon.

    abstract::To test the hypothesis that stress deprivation induces over-expression of cytokines in the patellar tendon, 40 rats were divided into the following two groups. In the stress-shielded group, we slackened the patellar tendon in the right knee by drawing the patella toward the tibial tubercle with flexible wires. In the ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2004.05.009

    authors: Uchida H,Tohyama H,Nagashima K,Ohba Y,Matsumoto H,Toyama Y,Yasuda K

    更新日期:2005-04-01 00:00:00

  • Mechanical characterisation of porcine rectus sheath under uniaxial and biaxial tension.

    abstract::Incisional hernia development is a significant complication after laparoscopic abdominal surgery. Intra-abdominal pressure (IAP) is known to initiate the extrusion of intestines through the abdominal wall, but there is limited data on the mechanics of IAP generation and the structural properties of rectus sheath. This...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2014.03.009

    authors: Lyons M,Winter DC,Simms CK

    更新日期:2014-06-03 00:00:00

  • On the assumption of steadiness of nasal cavity flow.

    abstract::The unsteady flow through a model of the human nasal cavity is analyzed at a Strouhal number of Sr=0.791 for the complete respiration cycle. A comparison of the essential flow structures in the model geometry and a real nasal cavity shows the relevance of the model data. The analysis of the steady and unsteady solutio...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2009.12.008

    authors: Hörschler I,Schröder W,Meinke M

    更新日期:2010-04-19 00:00:00

  • The effect of step-wise increased stretching on rat calvarial osteoblast collagen production.

    abstract::Mechanical forces regulate the function of bone cells. In this paper, the effects of cyclic stretching on osteoblasts derived from rat calvaria were studied at a magnitude occurring in physiological loaded bone tissue. A four-point bending apparatus was used to apply cyclic stretching on osteoblasts. Stretching at 500...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/s0021-9290(03)00237-9

    authors: Tang LL,Wang YL,Pan J,Cai SX

    更新日期:2004-01-01 00:00:00

  • Volume effects on fatigue life of equine cortical bone.

    abstract::Materials, including bone, often fail due to loading in the presence of critical flaws. The relative amount, location, and interaction of these flaws within a stressed volume of material play a role in determining the failure properties of the structure. As materials are generally imperfect, larger volumes of material...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2007.05.025

    authors: Bigley RF,Gibeling JC,Stover SM,Hazelwood SJ,Fyhrie DP,Martin RB

    更新日期:2007-01-01 00:00:00

  • Normal and shear stresses influence the spatial distribution of intracellular adhesion molecule-1 expression in human umbilical vein endothelial cells exposed to sudden expansion flow.

    abstract::Patterns in cell adhesion molecule expression by endothelial cells may play a role in atherogenesis. Previous studies have shown dependence of intracellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVEC) on shear stress and have indirectly linked ICAM-1 expression to spatial ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2005.02.002

    authors: McKinney VZ,Rinker KD,Truskey GA

    更新日期:2006-01-01 00:00:00

  • Toward quantification of athetotic movements by frequency spectrum analysis.

    abstract::The frequency spectrum of the elbow movements of one normal subject and six handicapped subjects were obtained in order to investigate the possibility of using this technique in quantifying athetosis. The frequency spectrum technique appears to be useful but data from more subjects must be obtained and specific detail...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/0021-9290(85)90046-6

    authors: Niku S,Henderson JM

    更新日期:1985-01-01 00:00:00

  • Regional dependency of the vascular smooth muscle cell contribution to the mechanical properties of the pig ascending aortic tissue.

    abstract:BACKGROUND:Dilation and dissection of aneurysmal ascending aortic tissues occur preferentially at the outer curvature of the vessel. In this study we hypothesize that the density and contractile properties of the vascular smooth muscle cells (VSMCs) of the pig ascending aorta (AA) are heterogeneous and could explain th...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2010.04.018

    authors: Tremblay D,Cartier R,Mongrain R,Leask RL

    更新日期:2010-08-26 00:00:00

  • A model-based parametric study of impact force during running.

    abstract::This paper deals with the impact force during foot-ground impact activities such as the running. A previously developed model is used for this study. The model is a lumped-parameter one consisting of four masses connected to each other via linear springs and viscous dampers. A shoe-specific nonlinear function is used ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2006.09.016

    authors: Zadpoor AA,Nikooyan AA,Arshi AR

    更新日期:2007-01-01 00:00:00

  • An improved method to assess torsional properties of rodent long bones.

    abstract::Torsion is an important testing modality commonly used to calculate structural properties of long bones. However, the effects of size and geometry must be excluded from the overall structural response in order to compare material properties of bones of different size, age and species. We have developed a new method to...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2009.04.019

    authors: Nazarian A,Entezari V,Vartanians V,Müller R,Snyder BD

    更新日期:2009-08-07 00:00:00

  • Effects of a sustained extension on arterial growth and remodeling: a theoretical study.

    abstract::Three recent studies reveal that the unloaded length of a carotid artery increases significantly and rapidly in response to sustained increases in axial extension. Moreover, such lengthening involves an "unprecedented" increase in the rate of turnover of cells and matrix. Although current data are not sufficient for d...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2004.06.017

    authors: Gleason RL,Humphrey JD

    更新日期:2005-06-01 00:00:00

  • Study on modelling of PMMA bone cement polymerisation.

    abstract::This contribution is concerned with the modelling of PMMA-based bone cement polymerisation process and the associated heat effect. While existing models use description in terms of the so-called polymerisation fraction to fit the experimental data, the new model is constructed in such a way as to mimic the chemical pr...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2004.06.021

    authors: Stańczyk M

    更新日期:2005-07-01 00:00:00

  • Apparent mass of the standing human body when using a whole-body vibration training machine: Effect of knee angle and input frequency.

    abstract::Several studies have investigated the transmission of vibration from the vibrating plate of a whole-body vibration training machine (WBVTM) to different locations on the human body. No known work has investigated the interface force between the vibrating plate of the machine and the human body. This paper investigates...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2018.11.003

    authors: Nawayseh N,Hamdan S

    更新日期:2019-01-03 00:00:00

  • Human crawling performance and technique revealed by inertial measurement units.

    abstract::Human crawling performance and technique are of broad interest to roboticists, biomechanists, and military personnel. This study explores the variables that define crawling performance in the context of an outdoor obstacle course used by military organizations worldwide to evaluate the effects of load and personal equ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2018.12.030

    authors: Vitali RV,Cain SM,Davidson SP,Perkins NC

    更新日期:2019-02-14 00:00:00

  • Fatigue creep damage at the cement-bone interface: an experimental and a micro-mechanical finite element study.

    abstract::The goal of this study was to quantify the micromechanics of the cement-bone interface under tensile fatigue loading using finite element analysis (FEA) and to understand the underlying mechanisms that play a role in the fatigue behavior of this interface. Laboratory cement-bone specimens were subjected to a tensile f...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2009.07.014

    authors: Waanders D,Janssen D,Miller MA,Mann KA,Verdonschot N

    更新日期:2009-11-13 00:00:00

  • Control of dynamic foot-ground interactions in male and female soccer athletes: females exhibit reduced dexterity and higher limb stiffness during landing.

    abstract::Controlling dynamic interactions between the lower limb and ground is important for skilled locomotion and may influence injury risk in athletes. It is well known that female athletes sustain anterior cruciate ligament (ACL) tears at higher rates than male athletes, and exhibit lower extremity biomechanics thought to ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2013.10.038

    authors: Lyle MA,Valero-Cuevas FJ,Gregor RJ,Powers CM

    更新日期:2014-01-22 00:00:00

  • Effect of the "squat protective response" on impact velocity during backward falls.

    abstract::Risk for injury during a fall depends on the position and velocity of the body segments at the moment of impact. One technique for reducing impact velocity is to absorb energy in the lower extremity muscles during descent, as occurs during squatting or sitting. However, the protective value of this response may depend...

    journal_title:Journal of biomechanics

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.jbiomech.2003.12.015

    authors: Robinovitch SN,Brumer R,Maurer J

    更新日期:2004-09-01 00:00:00

  • The poro-elastic behaviour of the intervertebral disc: A new perspective on diurnal fluid flow.

    abstract::Diurnal disc height changes, due to fluid in- and outflow, are in equilibrium while daytime spinal loading is twice as long as night time rest. A direction-dependent permeability of the endplates, favouring inflow over outflow, reportedly explains this; however, fluid flow through the annulus fibrosus should be consid...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2015.11.041

    authors: Vergroesen PA,van der Veen AJ,Emanuel KS,van Dieën JH,Smit TH

    更新日期:2016-04-11 00:00:00

  • Finite element investigation of the loading rate effect on the spinal load-sharing changes under impact conditions.

    abstract::Sudden deceleration and frontal/rear impact configurations involve rapid movements that can cause spinal injuries. This study aimed to investigate the rotation rate effect on the L2-L3 motion segment load-sharing and to identify which spinal structure is at risk of failure and at what rotation velocity the failure may...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2009.03.036

    authors: El-Rich M,Arnoux PJ,Wagnac E,Brunet C,Aubin CE

    更新日期:2009-06-19 00:00:00

  • Shear and pressure under the first ray in neuropathic diabetic patients: Implications for support of the longitudinal arch.

    abstract:OBJECTIVE:To assess dynamic arch support in diabetic patients at risk for Charcot neuroarthopathy whose arch index has not yet shown overt signs of foot collapse. METHODS:Two indirect measures of toe flexor activation (ratios: peak hallux pressure to peak metatarsal pressure - Ph/Pm; peak posterior hallux shear to pea...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2016.12.024

    authors: Davis B,Crow M,Berki V,Ciltea D

    更新日期:2017-02-08 00:00:00

  • On stiffness of scaffolds for bone tissue engineering-a numerical study.

    abstract::Tissue scaffolds are typically designed and fabricated to match native bone properties. However, it is unclear if this would lead to the best tissue ingrowth outcome within the scaffold as neo-tissue keeps changing the stiffness of entire construct. This paper presents a numerical method to address this issue for desi...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2010.02.020

    authors: Sturm S,Zhou S,Mai YW,Li Q

    更新日期:2010-06-18 00:00:00

  • Influence of microcalcifications on vulnerable plaque mechanics using FSI modeling.

    abstract::Sudden heart attacks remain one of the primary causes of premature death in the developed world. Asymptomatic vulnerable plaques that rupture are believed to prompt such fatal heart attacks and strokes. The role of microcalcifications in the vulnerable plaque rupture mechanics is still debated. Recent studies suggest ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2007.11.029

    authors: Bluestein D,Alemu Y,Avrahami I,Gharib M,Dumont K,Ricotta JJ,Einav S

    更新日期:2008-01-01 00:00:00

  • Effect of chronic idiopathic low back pain on the kinetic gait characteristics in different foot masks.

    abstract::Identification of kinetic variables in different masks of foot is important for the evaluation and treatment of chronic low back pain. The aim of this study was to investigate the effect of chronic idiopathic low back pain on kinetic variables of gait in different foot masks. 11 idiopathic chronic low back pain patien...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2018.08.013

    authors: Yazdani S,Dizji E,Alizadeh F,Hassanlouei H

    更新日期:2018-10-05 00:00:00

  • Sticky chain model for shear response of red blood cells.

    abstract::A sticky chain model has been proposed to describe the unfolding of spectrin network under applied mechanical loads. With the model, the response of a red blood cell (RBC) under static and cyclic shear loading has been predicted, which agrees qualitatively with relevant experimental results. ...

    journal_title:Journal of biomechanics

    pub_type: 杂志文章

    doi:10.1016/j.jbiomech.2008.05.028

    authors: Huang GY,Mai YW

    更新日期:2008-08-07 00:00:00