Abstract:
BACKGROUND:Persistent hyperlactatemia is associated with greater mortality in shock. Liver is the main site of lactate metabolism. METHOD:In the first part, freshly isolated hepatocytes were incubated in 10% fetal bovine serum William's E medium supplemented with 10 mM lactate. Cells were then exposed to 100 μM ursodeoxycholic acid (UDCA), with no addition (control) for 2, 4, 6, 8 h. In the second part, hepatocytes were treated with Silencer select siRNA targeting FXR or scramble siRNA. The siRNA treatment was repeated twenty four hours later, and the cells were used in the experiments twenty-four hours after the second treatment. Then hepatocytes were incubated in 10% fetal bovine serum William's E medium supplemented with 10 mM lactate. Cells were then exposed to 100 μM UDCA for 2, 4, 6, 8 h. Lactate concentration was determined by ABL80 automatic blood gas analyzer. RESULTS:UDCA increased ability of hepatocytes to remove lactate. After the knockdown of FXR, effects caused by UDCA were weakened. CONCLUSION:These results demonstrate that UDCA promotes lactate metabolism in mouse hepatocytes through CA-FXR pathway.
journal_name
Curr Mol Medjournal_title
Current molecular medicineauthors
Wang L,He HW,Zhou X,Long Ydoi
10.2174/1566524020666200123161340subject
Has Abstractpub_date
2020-01-01 00:00:00pages
661-666issue
8eissn
1566-5240issn
1875-5666pii
CMM-EPUB-103882journal_volume
20pub_type
杂志文章abstract::Central Nervous System (CNS) tuberculosis is a serious, often fatal form of tuberculosis, predominantly affecting young children. HIV co-infection and drug resistant strains of Mycobacterium tuberculosis are making the diagnosis and treatment of CNS tuberculosis more complicated. Current concepts about the pathogenesi...
journal_title:Current molecular medicine
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更新日期:2014-01-01 00:00:00
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更新日期:2017-01-01 00:00:00
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更新日期:2005-12-01 00:00:00
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更新日期:2002-06-01 00:00:00
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更新日期:2006-06-01 00:00:00
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更新日期:2010-03-01 00:00:00
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更新日期:2006-05-01 00:00:00
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更新日期:2012-12-01 00:00:00