Abstract：
:Extracellular vesicles (EVs) are shed by all eukaryotic cells and have emerged as important intercellular regulators. EVs released by osteoclasts were recently identified as important coupling factors in bone remodeling. They are shed as osteoclasts resorb bone and stimulate osteoblasts to form bone to replace the bone resorbed. We reported the proteomic content of osteoclast EVs with data from two-dimensional, high resolution liquid chromatography/mass spectrometry. In this article, we examine in detail the actin and actin-associated proteins found in osteoclast EVs. Like EVs from other cell types, actin and various actin-associated proteins were abundant. These include components of the polymerization machinery, myosin mechanoenzymes, proteins that stabilize or depolymerize microfilaments, and actin-associated proteins that are involved in regulating integrins. The selective incorporation of actin-associated proteins into osteoclast EVs suggests that they have roles in the formation of EVs and/or the regulatory signaling functions of the EVs. Regulating integrins so that they bind extracellular matrix tightly, in order to attach EVs to the extracellular matrix at specific locations in organs and tissues, is one potential active role for actin-associated proteins in EVs.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Holliday LS,Faria LP,Rody WJ Jrdoi
10.3390/ijms21010158subject
Has Abstractpub_date
2019-12-25 00:00:00issue
1issn
1422-0067pii
ijms21010158journal_volume
21pub_type
杂志文章,评审abstract：:Acoustic wave biosensors are a real-time, label-free biosensor technology, which have been exploited for the detection of proteins and cells. One of the conventional biosensor approaches involves the immobilization of a monolayer of antibodies onto the surface of the acoustic wave device for the detection of a specifi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9020154
更新日期：2008-02-01 00:00:00
abstract：:Polymer supported dichlorophosphate (PEG-OPOCl(2)) is an efficient green catalyst for the electrophilic substitution reaction of indole with aromatic aldehydes, in neat condition, to afford an excellent yield of bis(indolyl) methanes with short reaction time, at room temperature. The synthesized compounds and their an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14011843
更新日期：2013-01-16 00:00:00
abstract：:Squamous cell carcinoma (SCC) is the second commonest type of skin cancer, and SCCs make up about 90% of head and neck cancers (HNSCCs). HNSCCs harbor two frequent molecular alterations, namely, gain-of-function alterations of phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) and loss-of-...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186585
更新日期：2020-09-09 00:00:00
abstract：:Several studies have demonstrated that basic fibroblast growth factor (bFGF) can induce neural differentiation of mesenchymal stem cells. In this study, we investigated the neural differentiation of muscle-derived stem cells (MDSCs) following treatment with bFGF and ethosuximide, a small molecule used as an anticonvul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14046614
更新日期：2013-03-25 00:00:00
abstract：:A series of schizonepetin derivatives have been designed and synthesized in order to obtain potent antivirus agents. The antiviral activity against HSV-1 and influenza virus H3N2 as well as the cytotoxicity of these derivatives was evaluated by using cytopathic effect (CPE) inhibition assay in vitro. Compounds M2, M4,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140817193
更新日期：2013-08-20 00:00:00
abstract：:Melanoma is a lethal tumor because of its severe metastatic potential, and serine/threonine-protein kinase B-raf inhibitors (BRAFi) are used in patients harboring BRAF-mutation. Unfortunately, BRAFi induce resistance. Therefore, we tested the activity of onconase (ONC), a cytotoxic RNase variant, against BRAFi-resista...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235980
更新日期：2019-11-27 00:00:00
abstract：:MicroRNAs (miRNAs) are short, non-coding post-transcriptional gene regulators. In mammalian cells, mature miRNAs are produced from primary precursors (pri-miRNAs) using canonical protein machinery, which includes Drosha/DGCR8 and Dicer, or the non-canonical mirtron pathway. In plant cells, mature miRNAs are excised fr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19124075
更新日期：2018-12-17 00:00:00
abstract：:Uterine fibroids (UFs) are benign tumors of the female genital tract made of the smooth muscle of the uterus. UF growth depends mostly on the influence of the steroid hormones and selected growth factors. Transforming growth factor β (TGF-βs) is a polypeptide that consists of three isoforms: TGF-β1, TGF-β2, and TGF-β3...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18112435
更新日期：2017-11-17 00:00:00
abstract：:The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)-a ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102396
更新日期：2019-05-15 00:00:00
abstract：:Metallothioneins (MTs) are multifunctional proteins occurring throughout the animal kingdom. While the expression and transcriptional regulation of MTs is well-studied in vertebrates, the mechanism of MT activation is still unknown for most invertebrates. Therefore, we examined wMT-2 gene regulation and expression pat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061113
更新日期：2017-05-24 00:00:00
abstract：:Despite improvements in diagnosis and treatment, breast cancer is still the most common cancer type among non-smoking females. TGF-β can inhibit breast cancer development by inducing cell cycle arrest in both, cancer cells and, as part of a senescence program in normal human mammary epithelial cells (HMEC). Moreover, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071574
更新日期：2017-07-20 00:00:00
abstract：:It is an important but also a challenging analytical problem to understand the chemical composition and structure of prebiotic organic matter that is present in extraterrestrial materials. Its formation, evolution and content in the building blocks ("seeds") for more complex molecules, such as proteins and DNA, are ke...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17040439
更新日期：2016-03-24 00:00:00
abstract：:Malignancy often arises from sophisticated defects in the intricate molecular mechanisms of cells, rendering a complicated molecular ground to effectively target cancers. Resistance toward cell death and enhancement of cell survival are the common adaptations in cancer due to its infinite proliferative capacity. Exist...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20020372
更新日期：2019-01-16 00:00:00
abstract：BACKGROUND:Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials such as graphene oxide ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20143603
更新日期：2019-07-23 00:00:00
abstract：:The ongoing pandemic of coronavirus disease-2019 (COVID-19) is being caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The disease continues to present significant challenges to the health care systems around the world. This is primarily because of the lack of vaccines to protect against the infe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155224
更新日期：2020-07-23 00:00:00
abstract：:The members of the Burkholderia genus are characterized by high versatility and adaptability to various ecological niches. With the availability of the genome sequences of numerous species of Burkholderia, many studies have been conducted to elucidate the unique features of this exceptional group of bacteria. Genomic ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20010121
更新日期：2018-12-29 00:00:00
abstract：:The mitochondrial genome of the nematode Romanomermis culicivorax encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the consensus cloverleaf. The single tRNA nucleotidyltransferase of this organism is fully active on armless tRNAs, while the human counterpart ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239047
更新日期：2020-11-28 00:00:00
abstract：:Malondialdehyde (MAA) within a lipid pathway has been demonstrated to possess an important role in endothelial function that undergoes periodontitis and coronary heart disease (CHD) development. This study evaluated the impact of periodontitis, CHD, or a combination of both diseases (periodontitis + CHD) on salivary a...
journal_title：International journal of molecular sciences
pub_type： 临床试验,杂志文章
doi：10.3390/ijms20236061
更新日期：2019-12-01 00:00:00
abstract：:Inflammation and transforming growth factor-β1 (TGF-β1) contribute to the development of peritoneal fibrosis (PF), which is associated with peritoneal dialysis (PD). Astragalus membranaceus (Astragalus) has anti-inflammatory and anti-fibrotic effects in many diseases. The goal of this study was to determine the anti-f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150712959
更新日期：2014-07-22 00:00:00
abstract：:It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610999
更新日期：2014-06-18 00:00:00
abstract：:We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used fo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16035990
更新日期：2015-03-13 00:00:00
abstract：:Kidney transplant recipients frequently suffer from skin infections and malignancies, possibly due to the effects of long-term immunosuppressive therapy. While the relationships between immunosuppression and these pathological conditions have been widely investigated, little is known about the relative incidence and c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17081362
更新日期：2016-08-19 00:00:00
abstract：:A novel camptothecin analogue, (20S)-10,11-methylenedioxy-camptothecin (FL118), has been proven to show significant antitumor efficacy for a wide variety of solid tumors. However, the further development of FL118 is severely hindered due to its extremely poor water solubility and adverse side effects. Here, two series...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228495
更新日期：2020-11-11 00:00:00
abstract：:Polyphenolic compounds contained in cherry extract (CE) are well known for their antioxidant and anti-inflammatory properties. Unfortunately, most of these natural compounds have low oral bioavailability, reducing their widespread use. Here, different concentrations of polyphenol-rich CE from Tuscany (Italy), encapsul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071759
更新日期：2019-04-10 00:00:00
abstract：:Wild barley eibi1 mutant with HvABCG31 gene mutation has low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. To better understand how such a mutant plant survives, we performed a genome-wide gene expression analysis. The leaf transcriptomes between the near-isoge...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141020478
更新日期：2013-10-14 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. In order to understand the effect of the mutations in G6PD gene function and its ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050787
更新日期：2016-05-21 00:00:00
abstract：:The human papillomavirus type 16 (HPV16) life cycle can be divided into an early stage in which the HPV16 genomic DNA is replicated, and a late stage in which the HPV16 structural proteins are synthesized and virions are produced. A strong coupling between the viral life cycle and the differentiation state of the infe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18020366
更新日期：2017-02-09 00:00:00
abstract：:The regeneration of dental tissues is a still an unmet clinical need; in fact, no therapies have been completely successful in regenerating dental tissue complexes such as periodontium, which is also due to the lack of scaffolds that are able to guide and direct cell fate towards the reconstruction of different minera...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113604
更新日期：2018-11-15 00:00:00
abstract：:Inflammatory liver diseases in the absence of pathogens such as intoxication by xenobiotics, cholestatic liver injury, hepatic ischemia-reperfusion injury (I/R), non-alcoholic steatohepatitis (NASH), or alcoholic liver disease (ALD) remain threatening conditions demanding specific therapeutic options. Caused by variou...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103104
更新日期：2018-10-10 00:00:00
abstract：BACKGROUND:Gastritis is a prevalent disease and commonly classified into autoimmune (A), bacterial (B), and chemical (C) type gastritis. While the former two subtypes are associated with an increased risk of developing gastric intestinal adenocarcinoma, the latter subtype is not. In this study, we evaluated the capabil...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186652
更新日期：2020-09-11 00:00:00
