Abstract:
:The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity. In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components. Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens. Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers). With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge. Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation. An oscillation rheometer was used to investigate viscoelastic properties. Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average. The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation. All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores. All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots. Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns. The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin. In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
journal_name
J Mech Behav Biomed Materauthors
Komorowicz E,Balázs N,Tanka-Salamon A,Varga Z,Szabó L,Bóta A,Longstaff C,Kolev Kdoi
10.1016/j.jmbbm.2019.103459subject
Has Abstractpub_date
2020-02-01 00:00:00pages
103459eissn
1751-6161issn
1878-0180pii
S1751-6161(19)30759-3journal_volume
102pub_type
杂志文章abstract::We report linear and non-linear shear tests on rat skin, evidencing a strain-softening regime, from 1% to 50% strain, followed by a strong strain-hardening regime, leading to a 'deck chair-shaped' stress-strain curve. The strain-softening regime was never reported as such in the literature, possibly mistaken for the l...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.12.021
更新日期:2017-05-01 00:00:00
abstract::The use of an adhesive for mesh fixation in hernia repair reduces chronic pain and minimizes tissue damage in the patient. This study was designed to assess the adhesive properties of a medium-chain (n-butyl) cyanoacrylate glue applied as drops or as a spray in a biomechanical and histologic study. Both forms of glue ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.08.008
更新日期:2017-11-01 00:00:00
abstract::The objective of this study was to develop a nanoscratch technique that can be used to measure the in situ toughness of bone at micro/nanostructural levels. Among the currently possible techniques, the surface scratch test may be conducted on very small regions, thus exhibiting a potential in determining the in situ f...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2011.08.019
更新日期:2012-01-01 00:00:00
abstract::Trans femoral amputation is one of the most uncomfortable surgeries in patient׳s life, where the prosthesis consisting of a socket, knee joint, pylon and foot is used to do the walking activities. The artificial prosthetic knee joint imitates the functions of human knee to achieve the flexion-extension for the above k...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.06.002
更新日期:2015-10-01 00:00:00
abstract::The work proposes the development of heterogeneous bio-implants with the aim to minimize stress shielding effect and enhance bone ingrowth. Stress shielding in the implant can be minimized by reducing the overall stiffness of the implant, which is achieved here by varying the material based on stress distribution acro...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.07.029
更新日期:2018-11-01 00:00:00
abstract::In this paper, to characterize the mechanical properties of meniscus by considering its local microstructure, a novel nonlinear poroviscoelastic Finite Element (FE) model has been developed. To obtain the mechanical response of meniscus, indentation experiments were performed on bovine meniscus samples. The ramp-relax...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.09.023
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of post-curing method, printing layer thickness, and water storage on the mechanical properties and degree of conversion of a light-curing methacrylate based resin material (IMPRIMO® LC Splint), used for the fabrication of 3D printed occlusal splints and surgical guides. METHODS:96 bar...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.104254
更新日期:2021-03-01 00:00:00
abstract:OBJECTIVE:To introduce an experimental non-carious cervical lesion (NCCL) model for studying the influence of presence and type of stress (tension or compression) on acid effects involved in NCCL formation on the enamel near the cement-enamel junction (CEJ). METHODS:108 bovine incisors were cut into 18 × 3×3 mm3 beams...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.11.003
更新日期:2017-02-01 00:00:00
abstract::Impaction bone grafting is a method of restoring bone stock to patients who have suffered significant bone loss due to revision total hip surgery. The procedure requires morsellised cancellous bone (MCB) to be impacted into the site of bone loss in order to stabilise the prosthesis with the aim of long term resorption...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.11.002
更新日期:2018-02-01 00:00:00
abstract::Extensive remodeling of the myocardium is seen in a variety of cardiovascular diseases, including systemic hypertension. Stem cell therapy has been proposed to improve the clinical outcomes of hypertension, and we hypothesized that changes in mechanical properties of the myocardium would accompany the progression of d...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.11.041
更新日期:2016-06-01 00:00:00
abstract::Previous studies on knee biomechanics have mainly focused on the joint structure itself, largely neglecting the material properties of the muscles and connective tissues around the knee joint. Therefore, this study was purposed to conduct a systematic in vivo examination of the material properties of muscles, tendons,...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.103829
更新日期:2020-09-01 00:00:00
abstract::Some ultraviolet radiation (UV) is essential to the body as it stimulates the production of vitamin D, yet overexposure has deleterious consequences for the skin. UV induces structural and cellular changes across the different layers of skin tissue leading to mechanical and oxidative stress. Both are critical paramete...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.02.008
更新日期:2013-12-01 00:00:00
abstract::Considering the interest of the research community in the fatigue behavior of all-ceramic restorations and the time consumed in low-frequency cyclic fatigue tests, this study aimed to investigate the influence of the loading frequency on the zirconia fatigue strength. The biaxial flexural fatigue strength of Y-TZP dis...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.01.008
更新日期:2016-08-01 00:00:00
abstract::Brazil nut (Bertholletia excelsa) fruits are capable of resisting high mechanical forces when released from trees as tall as 50 m, as well as during animal dispersal by sharp-teethed rodents. Thick mesocarp plays a crucial part in seed protection. We investigated the role of microstructure and how sclereids, fibers, a...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.104306
更新日期:2021-01-07 00:00:00
abstract::In the present work, we propose the first structural constitutive model of the passive mechanical behavior of the swine colon that is validated against physiological inflation-extension tests, and accounts for residual strains. Sections from the spiral colon and the descending colon were considered to investigate pote...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.08.031
更新日期:2018-01-01 00:00:00
abstract::It is generally accepted that calcium phosphate (CaP) is one of the most important biomaterials in implant coating applications mainly because of its excellent bioactivity. However, its relatively poor mechanical properties limits its application. This entails that a better understanding of the mechanical properties o...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2010.01.002
更新日期:2010-05-01 00:00:00
abstract::Viscoelastic properties of the white matter brain tissue are systematically studied in this paper utilizing indentation experiments, mathematical modeling, and finite element simulation. It is first demonstrated that the internal stiffness of the instrument needs to be thoroughly obtained and incorporated in the analy...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.08.037
更新日期:2018-01-01 00:00:00
abstract::The structure, composition and morphology of a radio-frequency (RF) magnetron sputter-deposited dense nano-hydroxyapatite (HA) coating that was deposited on the surface of an AZ31 magnesium alloy were characterized using AFM, SEM, EDX and XRD. The results obtained from SEM and XRD experiments revealed that the bias ap...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.02.025
更新日期:2015-06-01 00:00:00
abstract::The current study is part of an ongoing research program to develop an advanced new carbon fiber/flax/epoxy (CF/flax/epoxy) hybrid composite with a “sandwich structure” as a substitute for metallic materials for orthopedic long bone fracture plate applications. The purpose of this study was to assess the fatigue prope...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Sylgard((R)) is a biocompatible elastomer which has been widely used in biomedical applications including in simulations of the mechanical response of soft tissues and mechanotransduction investigations. In this study the effect of fabrication parameters including base to curing agent ratio and curing time on the mech...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2010.04.003
更新日期:2010-08-01 00:00:00
abstract::Age-related aortic stiffening is associated with cardiovascular diseases such as heart failure. The mechanical functions of the main structural components of the aorta, such as collagen and elastin, are determined in part by their organisation at the micrometer length scale. With age and disease both components underg...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.07.018
更新日期:2016-12-01 00:00:00
abstract::As a substitute for 2,2-bis[4-(2-hydroxy-3- methacryloxypropoxy)phenyl] propane (Bis-GMA) in dental materials, a new dimethacrylate monomer TDDMMA without bisphenol-A structure was synthesized through the reaction between tricyclo[5.2.1.0(2,6)]- decanedimethanol and 2-isocyanatoethyl methacrylate. The TDDMMA was mixed...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.11.020
更新日期:2016-04-01 00:00:00
abstract::The hierarchical architecture of the collagen fibril is well understood, involving non-integer staggering of collagen molecules which results in a 67 nm periodic molecular density variation termed D-banding. Other than this variation, collagen fibrils are considered to be homogeneous at the micro-scale and beyond. Int...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.103849
更新日期:2020-10-01 00:00:00
abstract::Mechanical property data for brain tissue are needed to understand the biomechanics of neurological disorders and response of the brain to different mechanical and surgical forces. Most studies have characterized mechanical behavior of brain tissues over large regions or classified tissue properties for either gray or...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.08.026
更新日期:2014-01-01 00:00:00
abstract::Imprecise knowledge on the composition-processing-microstructure-property correlation of titanium alloys combined with experimental data are used for developing rule based models for predicting the strength and elastic modulus of titanium alloys. The developed models are used for designing alloys suitable for orthoped...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.08.039
更新日期:2016-01-01 00:00:00
abstract::Conductive hearing loss, due to middle ear pathologies or traumas, affects more than 5% of the population worldwide. Passive prostheses to replace the ossicular chain mainly rely on piston-like titanium and/or hydroxyapatite devices, which in the long term suffer from extrusion. Although the basic shape of such device...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2019.103541
更新日期:2020-03-01 00:00:00
abstract::Orthopedic interfaces such as the tendon-bone junction (TBJ) present unique challenges for biomaterials development. Here we describe a multi-compartment collagen-GAG scaffold fabricated via lyophilization that contains discrete mineralized (CGCaP) and non-mineralized (CG) regions joined by a continuous interface. Mod...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.07.016
更新日期:2013-12-01 00:00:00
abstract::The goal of this study was to investigate the effect of resveratrol treatment on the osteogenic potential of human and rat adipose derived stem cells in a 3-D culture environment. Adipose derived stem cells (ADSCs) have been widely studied and have shown promise as a potential source of osteogenic progenitor cells. Pr...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2011.08.014
更新日期:2012-07-01 00:00:00
abstract::The purpose of this study was to evaluate LC Bead LUMI™ (40-90µm and 70-150µm) in order to determine if their increased resistance to compression influences microsphere penetration and distribution compared to more compressible commercial microspheres. LC Bead LUMI™ 40-90µm and 70-150µm, LC BeadM1® 70-150µm, Embozene™...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.10.034
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:To investigate the elastic properties of human enamel using resonant ultrasound spectroscopy (RUS). METHODS:Six rectangular parallelepiped specimens were prepared from six human third molars. For all specimens, the theoretical resonant frequencies were calculated using the Rayleigh-Ritz method, knowing the ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.09.014
更新日期:2019-01-01 00:00:00