Overcoming chromatin barriers.

abstract：:The yeast Dsc E3 ligase complex has long been recognized as a Golgi-specific protein ubquitination system. It shares a striking sequence similarity to the Hrd1 complex that plays critical roles in the ER-associated degradation pathway. Using biochemical purification and mass spectrometry, we identified two novel Dsc s...

journal_title：eLife

authors： Yang X,Arines FM,Zhang W,Li M

更新日期：2018-01-22 00:00:00

abstract：:The Fluc family of F(-) ion channels protects prokaryotes and lower eukaryotes from the toxicity of environmental F(-). In bacteria, these channels are built as dual-topology dimers whereby the two subunits assemble in antiparallel transmembrane orientation. Recent crystal structures suggested that Fluc channels conta...

journal_title：eLife

authors： Last NB,Kolmakova-Partensky L,Shane T,Miller C

更新日期：2016-07-23 00:00:00

abstract：:Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...

journal_title：eLife

authors： Ma H,O'Farrell PH

更新日期：2015-08-03 00:00:00

abstract：:Surveillance strategies based on whole genome sequencing could help with the early identification and detection of new forms of drug-resistant gonorrhoea. ...

journal_title：eLife

authors： Medland N

更新日期：2020-06-30 00:00:00

abstract：:Minor and major spliceosomes control splicing of distinct intron types and are thought to act largely independent of one another. SR proteins are essential splicing regulators mostly connected to the major spliceosome. Here, we show that Srsf10 expression is controlled through an autoregulated minor intron, tightly co...

journal_title：eLife

authors： Meinke S,Goldammer G,Weber AI,Tarabykin V,Neumann A,Preussner M,Heyd F

更新日期：2020-04-27 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. The region is implicated in a wide range of behaviors and several modeling studies have explored its circuit computations. Most studies have relied on assum...

journal_title：eLife

authors： Franconville R,Beron C,Jayaraman V

更新日期：2018-08-20 00:00:00

abstract：:Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately...

journal_title：eLife

authors： Schaich MA,Sanford SL,Welfer GA,Johnson SA,Khoang TH,Opresko PL,Freudenthal BD

更新日期：2020-06-05 00:00:00

abstract：:Bacterial cells are encased in a peptidoglycan (PG) exoskeleton that protects them from osmotic lysis and specifies their distinct shapes. Cell wall hydrolases are required to enlarge this covalently closed macromolecule during growth, but how these autolytic enzymes are regulated remains poorly understood. Bacillus s...

journal_title：eLife

authors： Dobihal GS,Brunet YR,Flores-Kim J,Rudner DZ

更新日期：2019-12-06 00:00:00

abstract：:Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether 'deep' sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011-201...

journal_title：eLife

authors： Lee RS,Proulx JF,McIntosh F,Behr MA,Hanage WP

更新日期：2020-02-04 00:00:00

abstract：:Mutations in the polycystin genes, PKD1 or PKD2, results in Autosomal Dominant Polycystic Kidney Disease (ADPKD). Although a genetic basis of ADPKD is established, we lack a clear understanding of polycystin proteins' functions as ion channels. This question remains unsolved largely because polycystins localize to the...

journal_title：eLife

authors： Liu X,Vien T,Duan J,Sheu SH,DeCaen PG,Clapham DE

更新日期：2018-02-14 00:00:00

abstract：:The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic integrity by responding to DNA damage through posttranslational m...

journal_title：eLife

authors： Papp SJ,Huber AL,Jordan SD,Kriebs A,Nguyen M,Moresco JJ,Yates JR,Lamia KA

更新日期：2015-03-10 00:00:00

abstract：:Forebrain precursor cells are dynamic during early brain development, yet the underlying molecular changes remain elusive. We observed major differences in transcriptional signatures of precursor cells from mouse forebrain at embryonic days E8.5 vs. E10.5 (before vs. after neural tube closure). Genes encoding protein ...

journal_title：eLife

authors： Chau KF,Shannon ML,Fame RM,Fonseca E,Mullan H,Johnson MB,Sendamarai AK,Springel MW,Laurent B,Lehtinen MK

更新日期：2018-05-10 00:00:00

abstract：:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....

journal_title：eLife

authors： Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

更新日期：2014-11-17 00:00:00

abstract：:Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocy...

journal_title：eLife

authors： Manzoni G,Marinach C,Topçu S,Briquet S,Grand M,Tolle M,Gransagne M,Lescar J,Andolina C,Franetich JF,Zeisel MB,Huby T,Rubinstein E,Snounou G,Mazier D,Nosten F,Baumert TF,Silvie O

更新日期：2017-05-16 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease involving cytotoxic conformations of Cu, Zn superoxide dismutase (SOD1). A major challenge in understanding ALS disease pathology has been the identification and atomic-level characterization of these conformers. Here, we use a combination ...

journal_title：eLife

authors： Sekhar A,Rumfeldt JA,Broom HR,Doyle CM,Bouvignies G,Meiering EM,Kay LE

更新日期：2015-06-23 00:00:00

abstract：:Experiments on flies suggest that a gain-of-function mechanism in a protein called CSPɑ contributes to the progressive brain disease CLN4. ...

journal_title：eLife

authors： Berryer MH,Kosmaczewski SG,Barrett LE

更新日期：2019-12-06 00:00:00

abstract：:Although pain is a common symptom of various diseases and disorders, its contribution to disease pathogenesis is not well understood. Here we show using murine experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS), that pain induces EAE relapse. Mechanistic analysis showed that pain indu...

journal_title：eLife

authors： Arima Y,Kamimura D,Atsumi T,Harada M,Kawamoto T,Nishikawa N,Stofkova A,Ohki T,Higuchi K,Morimoto Y,Wieghofer P,Okada Y,Mori Y,Sakoda S,Saika S,Yoshioka Y,Komuro I,Yamashita T,Hirano T,Prinz M,Murakami M

更新日期：2015-08-11 00:00:00

abstract：:Hippocampal oscillations are dynamic, with unique oscillatory frequencies present during different behavioral states. To examine the extent to which these oscillations reflect neuron engagement in distinct local circuit processes that are important for memory, we recorded single cell and local field potential activity...

journal_title：eLife

authors： Rangel LM,Rueckemann JW,Riviere PD,Keefe KR,Porter BS,Heimbuch IS,Budlong CH,Eichenbaum H

更新日期：2016-01-11 00:00:00

abstract：:Physiological adaptation to proteotoxic stress in the endoplasmic reticulum (ER) requires retrotranslocation of misfolded proteins into the cytoplasm for ubiquitination and elimination by ER-associated degradation (ERAD). A surprising paradox emerging from recent studies is that ubiquitin ligases (E3s) and deubiquitin...

journal_title：eLife

authors： Liu Y,Soetandyo N,Lee JG,Liu L,Xu Y,Clemons WM Jr,Ye Y

更新日期：2014-01-01 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the enzymatic activities of the replicative DNA Pol III are well understood, its dynamics within the replisome are not. Here, we test the accepted v...

journal_title：eLife

authors： Lewis JS,Spenkelink LM,Jergic S,Wood EA,Monachino E,Horan NP,Duderstadt KE,Cox MM,Robinson A,Dixon NE,van Oijen AM

更新日期：2017-04-22 00:00:00

abstract：:Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired. ...

journal_title：eLife

authors： Gibb B,Greene EC

更新日期：2012-12-13 00:00:00

abstract：:The ability to flexibly navigate an environment relies on a hippocampal-dependent cognitive map. External space can be internally mapped at different spatial resolutions. However, whether hippocampal spatial coding resolution can rapidly adapt to local features of an environment remains unclear. To explore this possib...

journal_title：eLife

authors： Bourboulou R,Marti G,Michon FX,El Feghaly E,Nouguier M,Robbe D,Koenig J,Epsztein J

更新日期：2019-03-01 00:00:00

abstract：:Mutations in PROP1 are the most common cause of hypopituitarism in humans; therefore, unraveling its mechanism of action is highly relevant from a therapeutic perspective. Our current understanding of the role of PROP1 in the pituitary gland is limited to the repression and activation of the pituitary transcription fa...

journal_title：eLife

authors： Pérez Millán MI,Brinkmeier ML,Mortensen AH,Camper SA

更新日期：2016-06-28 00:00:00

abstract：:Multi-modal MRI techniques have identified biomarkers that could help to predict whether someone will develop epilepsy. ...

journal_title：eLife

authors： Gröhn O,Sierra A

更新日期：2017-07-26 00:00:00

abstract：:Hybrid sterility is one of the reproductive isolation mechanisms leading to speciation. Prdm9, the only known vertebrate hybrid-sterility gene, causes failure of meiotic chromosome synapsis and infertility in male hybrids that are the offspring of two mouse subspecies. Within species, Prdm9 determines the sites of pro...

journal_title：eLife

authors： Gregorova S,Gergelits V,Chvatalova I,Bhattacharyya T,Valiskova B,Fotopulosova V,Jansa P,Wiatrowska D,Forejt J

更新日期：2018-03-14 00:00:00

abstract：:Uromodulin is the most abundant protein in the urine. It is exclusively produced by renal epithelial cells and it plays key roles in kidney function and disease. Uromodulin mainly exerts its function as an extracellular matrix whose assembly depends on a conserved, specific proteolytic cleavage leading to conformation...

journal_title：eLife

authors： Brunati M,Perucca S,Han L,Cattaneo A,Consolato F,Andolfo A,Schaeffer C,Olinger E,Peng J,Santambrogio S,Perrier R,Li S,Bokhove M,Bachi A,Hummler E,Devuyst O,Wu Q,Jovine L,Rampoldi L

更新日期：2015-12-17 00:00:00

abstract：:Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...

journal_title：eLife

authors： Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

更新日期：2016-01-18 00:00:00

abstract：:Humans and other animals routinely identify and attend to sensory stimuli so as to rapidly acquire rewards or avoid aversive experiences. Emotional arousal, a process mediated by the amygdala, can enhance attention to stimuli in a non-spatial manner. However, amygdala neural activity was recently shown to encode spati...

journal_title：eLife

authors： Peck CJ,Salzman CD

更新日期：2014-10-30 00:00:00