Abstract:
:In meiosis, multiple different DNA sequence motifs help to position homologous recombination at hotspots in the genome. How do the seemingly disparate cis-acting regulatory modules each promote locally the activity of the basal recombination machinery? We defined molecular mechanisms of action for five different hotspot-activating DNA motifs (M26, CCAAT, Oligo-C, 4095, 4156) located independently at the same site within the ade6 locus of the fission yeast Schizosaccharomyces pombe Each motif promoted meiotic recombination (i.e., is active) within this context, and this activity required the respective binding proteins (transcription factors Atf1, Pcr1, Php2, Php3, Php5, Rst2). High-resolution analyses of chromatin structure by nucleosome scanning assays revealed that each motif triggers the displacement of nucleosomes surrounding the hotspot motif in meiosis. This chromatin remodeling required the respective sequence-specific binding proteins, was constitutive for two motifs, and was enhanced meiotically for three others. Hotspot activity of each motif strongly required the ATP-dependent chromatin remodeling enzyme Snf22 (Snf2/Swi2), with lesser dependence on Gcn5, Mst2, and Hrp3. These findings support a model in which most meiotic recombination hotspots are positioned by the binding of transcription factors to their respective DNA sites. The functional redundancy of multiple, sequence-specific protein-DNA complexes converges upon shared chromatin remodeling pathways that help provide the basal recombination machinery (Spo11/Rec12 complex) access to its DNA substrates within chromatin.
journal_name
Geneticsjournal_title
Geneticsauthors
Mukiza TO,Protacio RU,Davidson MK,Steiner WW,Wahls WPdoi
10.1534/genetics.119.302679subject
Has Abstractpub_date
2019-11-01 00:00:00pages
789-803issue
3eissn
0016-6731issn
1943-2631pii
genetics.119.302679journal_volume
213pub_type
杂志文章相关文献
GENETICS文献大全abstract::A new suppressor of +1 frameshift mutations has been isolated in Salmonella typhimurium. This suppressor, sufJ, maps at minute 89 on the Salmonella genetic map between the argH and rpo(rif) loci, closely linked to the gene for the ochre suppressor tyrU(supM). The suppressor mutation is dominant to its wild-type allele...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::The rate of adaptive evolution of a population ultimately depends on the rate of incorporation of beneficial mutations. Even beneficial mutations may, however, be lost from a population since mutant individuals may, by chance, fail to reproduce. In this paper, we calculate the probability of fixation of beneficial mut...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::The identification of genes affecting sociality can give insights into the maintenance and development of sociality and personality. In this study, we used the combination of an advanced intercross between wild and domestic chickens with a combined QTL and eQTL genetical genomics approach to identify genes for social ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300810
更新日期:2018-05-01 00:00:00
abstract::We explore the effects of demography and linkage on a maximum-likelihood (ML) method for estimating selection and mutation parameters in a reversible mutation model. This method assumes free recombination between sites and a randomly mating population of constant size and uses information from both polymorphic and mon...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.122150
更新日期:2010-12-01 00:00:00
abstract::Reciprocal recurrent selection (RRS), which assumes overdominant loci to be important, alters two genetically different populations to improve their crossbred mean. Individual plants from two populations (A and B) are selfed and also crossed with plants from the reciprocal female tester population (B and A, respective...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the cultivated tomato, Lycopersicon esculentum, and the closely related L. pimpinellifolium is triggered by the physical interaction between plant disease resistance protein, Pto, and the pathogen avirulence protein, AvrPto. To inves...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.039339
更新日期:2005-09-01 00:00:00
abstract::Spt3 of Saccharomyces cerevisiae is required for the normal transcription of many genes in vivo. Past studies have shown that Spt3 is required for both mating and sporulation, two events that initiate when cells are at G(1)/START. We now show that Spt3 is needed for two other events that begin at G(1)/START, diploid f...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::The bristle worm Platynereis dumerilii displays many interesting biological characteristics. These include its reproductive timing, which is synchronized to the moon phase, its regenerative capacity that is hormonally controlled, and a slow rate of evolution, which permits analyses of ancestral genes and cell types. A...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.112.148254
更新日期:2014-05-01 00:00:00
abstract::The parentals of the interspecific cross Cucurbita maxima xC. ecuadorensis had different isozyme phenotypes for 12 enzyme systems. Characterization of the systems demonstrated that the expression and intracellular distribution of the isozymes were similar to those in other plant taxa; however, a considerable number of...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::The F(1) hybrids of Nicotiana suaveolens (subgenus Petunioides, 2n = 32) and N. glutinosa (subgenus Tabacum, 2n = 24), were examined during their development, from seedlings to mature plants. It was observed that in the hybrids, there was a progressive change of dominant N. glutinosa morphological characteristics towa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-04-01 00:00:00
abstract::The GAL4 protein of Saccharomyces cerevisiae binds to DNA upstream of each of six genes and stimulates their transcription. To locate regions of the protein responsible for these processes, we identified and characterized 88 gal4 mutations selected in vivo to reduce the ability to GAL4 protein to activate transcriptio...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
abstract::The maize ligule is an adaxial membranous structure on the leaf that develops at the boundary of the sheath and blade. The ligule and the associated auricle are dispensable structures, amenable to genetic manipulation. We present here a genetic analysis of liguleless1 (lg1) and liguleless2 (lg2), the two genes known t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Transmission ratio distortion (TRD) is the departure from the expected genotypic frequencies under Mendelian inheritance. This departure can be due to multiple physiological mechanisms during gametogenesis, fertilization, fetal and embryonic development, and early neonatal life. Although a few TRD loci have been repor...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.135988
更新日期:2012-05-01 00:00:00
abstract::The molecular basis of the maternally inherited, heteroplasmic NCS2 mutant of maize was investigated. Analysis of the NCS2 mtDNA showed that it closely resembles the progenitor cmsT mitochondrial genome, except that the mutant genome contains a fused nad4-nad7 gene and is deleted for the small fourth exon of nad4. The...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::To develop a catalog of regulatory sites in two major model organisms, Drosophila melanogaster and Caenorhabditis elegans, the modERN (model organism Encyclopedia of Regulatory Networks) consortium has systematically assayed the binding sites of transcription factors (TFs). Combined with data produced by our predecess...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300657
更新日期:2018-03-01 00:00:00
abstract::Inactivation of the maternal or paternal X chromosome in a mammalian embryonic XX cell is believed to involve random choice between the two X's. We propose two alternative models. One suggests that choice is not random, while the other is consistent with random choice, but not one between two X's. ...
journal_title:Genetics
pub_type: 评论,杂志文章
doi:10.1534/genetics.104.029819
更新日期:2004-07-01 00:00:00
abstract::An SSR-based linkage map was constructed in Brassica rapa. It includes 113 SSR, 87 RFLP, and 62 RAPD markers. It consists of 10 linkage groups with a total distance of 1005.5 cM and an average distance of 3.7 cM. SSRs are distributed throughout the linkage groups at an average of 8.7 cM. Synteny between B. rapa and a ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.038968
更新日期:2006-05-01 00:00:00
abstract::Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically si...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::Precocious entry into S phase due to overproduction of G1 regulators can cause genomic instability. The mechanisms of this phenomenon are largely unknown. We explored the consequences of precocious S phase in yeast by overproducing a deregulated form of Swi4 (Swi4-t). Swi4 is a late G1-specific transcriptional activat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The extent to which epistasis contributes to adaptation, population differentiation, and speciation is a long-standing and important problem in evolutionary genetics. Using recombinant inbred (RI) lines of Arabidopsis thaliana grown under natural field conditions, we have examined the genetic architecture of fitness-c...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.046078
更新日期:2005-12-01 00:00:00
abstract::Segregation data were obtained for 1260 potato linkage group I-specific AFLP loci from a heterozygous diploid potato population. Analytical tools that identified potential typing errors and/or inconsistencies in the data and that assembled cosegregating markers into bins were applied. Bins contain multiple-marker data...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::The developmental gene optomotor-blind (omb) encodes a T-box-containing transcription factor that has multiple roles in Drosophila development. Previous genetic analyses established that omb plays a key role in establishing the abdominal pigmentation pattern of Drosophila melanogaster. In this report we examine patter...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.032078
更新日期:2004-12-01 00:00:00
abstract::A multivariate quantitative genetic model is analyzed that is based on the assumption that the genetic variation at a locus j primarily influences an underlying physiological variable yj, while influence on the genotypic values is determined by a kind of "developmental function" which is not changed by mutations at th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::The eukaryotic cytoplasm contains a variety of ribonucleoprotein (RNP) granules in addition to the better-understood membrane-bound organelles. These granules form in response to specific stress conditions and contain a number of signaling molecules important for the control of cell growth and survival. However, relat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.187419
更新日期:2016-07-01 00:00:00
abstract::The cut gene in Drosophila is necessary in at least one cell type, the external sensory organs, for proper cell type specification and morphogenesis. It is also expressed in a variety of other tissues, where its function is less well characterized. Previous work has demonstrated that mutations affecting all the tissue...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::We test models for the evolution of helical regions of RNA sequences, where the base pairing constraint leads to correlated compensatory substitutions occurring on either side of the pair. These models are of three types: 6-state models include only the four Watson-Crick pairs plus GU and UG; 7-state models include a ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Mounting evidence suggests that natural populations can harbor extensive fitness diversity with numerous genomic loci under selection. It is also known that genealogical trees for populations under selection are quantifiably different from those expected under neutral evolution and described statistically by Kingman's...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.160986
更新日期:2014-07-01 00:00:00
abstract::Genic heterozygosity and variation were studied in the permanent translocation heterozygotes Oenothera biennis I, Oe. biennis II, Oe. biennis III, Oe. strigosa, Oe. parviflora I, Oe. parviflora II, and in the related bivalent formers Oe. argillicola and Oe. hookeri. From variation at 20 enzyme loci, we find that trans...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::Most studies of mutation rates implicitly assume that they remain constant throughout development of the germline. However, researchers recently used a novel statistical framework to reveal that mutation rates differ dramatically during sperm development in Drosophila melanogaster. Here a general framework is describe...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.151571
更新日期:2013-08-01 00:00:00
abstract::Grain yield is a major goal for the improvement of durum wheat, particularly in drought-prone areas. In this study, the genetic basis of grain yield (GY), heading date (HD), and plant height (PH) was investigated in a durum wheat population of 249 recombinant inbred lines evaluated in 16 environments (10 rainfed and 6...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077297
更新日期:2008-01-01 00:00:00