Isoflavone accumulation and the metabolic gene expression in response to persistent UV-B irradiation in soybean sprouts.

Abstract:

:This study investigated the effects of persistent ultraviolet B (UV-B) irradiation on isoflavone accumulation in soybean sprouts. Three malonyl isoflavones were increased by UV-B. Malonylgenistin specifically accumulated upon UV-B exposure, whereas the other isoflavones were significantly increased under both dark conditions and UV-B exposure. The results of isoflavone accumulation to UV-B irradiation time were observed as following: acetyl glycitin rapidly increased and then gradually decreased; malonyl daidzin and malonyl genistin were highly accumulated within an intermediate period; genistein and daidzin were gradually maximized; daidzin, glycitin, genistein, and malonyl glycitin did not increase; and glycitin, acetyl daidzin, and acetyl genistin exhibited trace amounts. Transcriptional analysis of isoflavonoid biosynthetic genes demonstrated that most metabolic genes were highly activated in response to UV-B 24 and UV-B 36 treatments. In particular, it was found that GmCHS6, GmCHS7, and GmCHS8 genes among the eight known genes encoding chalcone synthase were specifically related to UV-B response.

journal_name

Food Chem

journal_title

Food chemistry

authors

Lim YJ,Jeong HY,Gil CS,Kwon SJ,Na JK,Lee C,Eom SH

doi

10.1016/j.foodchem.2019.125376

subject

Has Abstract

pub_date

2020-01-15 00:00:00

pages

125376

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(19)31490-6

journal_volume

303

pub_type

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