Abstract：
:Grapes are a great source of phenolic compounds, which have excellent antioxidant properties. Efficient analytical methods are necessary to selectively and precisely determine these compounds content in grapes. In this study, a reverse-phase ultra-high performance liquid chromatography (UHPLC) method with fluorescence and photodiode array detection has been developed to determine and quantify 27 of the main phenolic compounds present in grapes. An ACQUITY UPLC® BEH C18 (50 mm × 2.1 mm i.d., 1.7 mm particle size) column was employed. A gradient method was developed and column temperature (25-55 °C), as well as flow rate (0.6-0.75 mL min-1), were optimized. The optimum conditions allowed the separation of all the compounds in less than 9 min. The method was validated and demonstrated excellent detection and quantification limits, precision, and selectivity. Finally, several grape varieties were studied in order to demonstrate the applicability of the method to the analysis of real matrix samples.
journal_name
Food Chemjournal_title
Food chemistryauthors
V González de Peredo A,Vázquez-Espinosa M,Piñeiro Z,Espada-Bellido E,Ferreiro-González M,F Barbero G,Palma Mdoi
10.1016/j.foodchem.2020.127569subject
Has Abstractpub_date
2021-01-01 00:00:00pages
127569eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31431-Xjournal_volume
334pub_type
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