Abstract：
:Tumour growth and metastatic colonization is strongly influenced by the tumour stroma, including cancer-associated fibroblasts (CAF). Multipotent mesenchymal stromal cells (MSC) are a possible source of CAF following myofibroblastic differentiation, and we have previously shown that MSC support tumour growth. Triggered by tumour cell-derived factors like transforming growth factor β1 (TGF-β1), myofibroblastic MSC differentiation is associated with the increased expression of markers including alpha smooth muscle actin (α-SMA). Here we show that myocardin-related transcription factor A (MRTF-A) plays an important role in myofibroblastic differentiation of primary human MSC in vitro and their tumour-supporting function in vivo. Recombinant TGF-β1 or tumour cell conditioned medium (TCM) elevated α-SMA, calponin 1 and collagen 1 A1 (COL1A1) amount on mRNA and protein level in MSC. This correlated with increased MRTF-A activity during MSC differentiation. MRTF-A knockdown by siRNA or shRNA impaired TGF-β1 and TCM induction of α-SMA and calponin 1, but not of COL1A1. Mixed xenograft experiments using HCT8 colorectal carcinoma cells and primary MSC of different donors revealed a significant reduction in tumour weight and volume upon MRTF-A knockdown in MSC. Our study suggests that MRTF-A is involved in the functional differentiation of MSC towards a tumour-promoting CAF phenotype in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Werner S,Lützkendorf J,Müller T,Müller LP,Posern Gdoi
10.1038/s41598-019-48142-zsubject
Has Abstractpub_date
2019-08-13 00:00:00pages
11725issue
1issn
2045-2322pii
10.1038/s41598-019-48142-zjournal_volume
9pub_type
杂志文章abstract：:PEA3 transcription factor subfamily is present in a variety of tissues with branching morphogenesis, and play a particularly significant role in neural circuit formation and specificity. Many target genes in axon guidance and cell-cell adhesion pathways have been identified for Pea3 transcription factor (but not for E...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75089-3
更新日期：2020-10-23 00:00:00
abstract：:The human β-defensin-1 (HBD1) is an antimicrobial peptide constitutively expressed by epithelial cells at mucosal surfaces. In addition to its microbicidal properties, the loss of HBD1 expression in several cancers suggests that it may also have an anti-tumor activity. Here, we investigated the link between HBD1 expre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36387-z
更新日期：2018-12-21 00:00:00
abstract：:Neuroticism is a personality trait, which is an important risk factor for psychiatric disorders. Recent genome-wide studies reported about 600 genes potentially influencing neuroticism. Little is known about the mechanisms of their action. Here, we aimed to conduct a more detailed analysis of genes that can regulate t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-82123-5
更新日期：2021-01-28 00:00:00
abstract：:Cisplatin (CDDP) is potent anticancer agent used for several tumor types. Thymoquinone (TQ) is naturally occurring compound drawing great attention as anticancer and chemomodulator for chemotherapies. Herein, we studied the potential cytotoxicity of thymoquinone, CDDP and their combination against human oral squamous ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13357-5
更新日期：2017-10-13 00:00:00
abstract：:Unambiguous identification of trace amounts of biochemical molecules in a complex background using terahertz spectroscopy is extremely challenging owing to the extremely small absorption cross sections of these molecules in the terahertz regime. Herein, we numerically propose a terahertz nonresonant nano-slits structu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60732-w
更新日期：2020-02-28 00:00:00
abstract：:Organotypic, three-dimensional (3D) cancer models have enabled investigations of complex microtissues in increasingly realistic conditions. However, a drawback of these advanced models remains the poor biological relevance of cancer cell lines, while higher clinical significance would be obtainable with patient-derive...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06544-x
更新日期：2017-07-26 00:00:00
abstract：:Magnetic resonance imaging (MRI) has evolved into an outstandingly versatile diagnostic modality, as it has the ability to non-invasively produce detailed information on a tissue's structure and function. Complementary data is normally obtained in separate measurements, either as contrast-weighted images, which are fa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44832-w
更新日期：2019-06-11 00:00:00
abstract：:Since fatigue is prevalent in modern societies, it is necessary to clarify the neural mechanisms of fatigue. The regulation of performance through fatigue sensation is one of the mechanisms that decreases performance in fatigue. However, it is unknown whether subjective feeling of fatigue is necessary for the regulati...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-017-16439-6
更新日期：2017-11-23 00:00:00
abstract：:Soil microorganisms play a crucial role in the biogeochemical cycling of nutrient elements and maintaining soil health. We aimed to investigate the response of bacteria communities to organic farming over different crops (rice, tea and vegetable) along the middle and lower reaches of the Yangtze River of China. Compar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35046
更新日期：2016-10-11 00:00:00
abstract：:Despite a long history, the clinical efficacy of cupping therapy is still under debate. This is likely due to the lack of direct evidence for the biological actions of cupping, since the short exposure of cells to vacuum condition rarely has affects cellular activity. In this study, the medicinal properties of a recen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40086-8
更新日期：2019-03-07 00:00:00
abstract：:Monolayer molybdenum disulfide (MoS2) has recently attracted intense interests due to its remarkable optical properties of valley-selected optical response, strong nonlinear wave mixing and photocurrent/photovoltaic generation and many corresponding potential applications. However, the nature of atomic-thin thickness ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23626
更新日期：2016-03-31 00:00:00
abstract：:We report on a combined photoluminescence imaging and atomic force microscopy study of single, isolated self-assembled InAs quantum dots. The motivation of this work is to determine an approach that allows to assess single quantum dots as candidates for quantum nanophotonic devices. By combining optical and scanning p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06566-5
更新日期：2017-07-24 00:00:00
abstract：:Utilization of computational approach in the study of social behaviour of animals is increasing and we attempted such an approach in our study of tree-dwelling bats. These bats live in highly dynamic fission-fusion societies that share multiple roosts in a common home range. The key behavioural component associated wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72999-0
更新日期：2020-10-08 00:00:00
abstract：:Understanding cancer-prone environments is important to efficiently detect and prevent cancers. The associations between miRNA and cancer-prone environments are still largely unknown in gastric cancer (GC). Six miRNAs that are differentially expressed during gastric carcinogenesis were selected, and quantitative real-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63230-1
更新日期：2020-04-20 00:00:00
abstract：:Regional brain sizes of very-preterm infants at term-equivalent age differ from those of term-born peers, which have been linked with later cognitive impairments. However, dependence of regional brain volume loss on gestational age has not been studied in detail. To investigate the spatial pattern of brain growth in n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33995
更新日期：2016-09-23 00:00:00
abstract：:Epilepsy is one of the most common neurological disorders and the administration of antiepileptic drugs (AEDs) is the most common treatment. Although there are more than 15 AEDs available, a third of epilepsy patients remain refractory to available drugs, so novel effective drugs are needed. Here, we found that DV21, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01734-z
更新日期：2017-05-10 00:00:00
abstract：:Converging evidence supports the involvement of pro-inflammatory pathways and the gut microbiome in major depressive disorder (MDD). Pre-clinical and clinical studies suggest that decreasing pro-inflammatory signaling may provide clinical benefit in MDD. In this study, we used the chronic unpredictable stress (CUS) pa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38055-8
更新日期：2019-04-23 00:00:00
abstract：:Hyperspectral imaging (HSI) is being explored in endoscopy as a tool to extract biochemical information that may improve contrast for early cancer detection in the gastrointestinal tract. Motion artefacts during medical endoscopy have traditionally limited HSI application, however, recent developments in the field hav...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60574-6
更新日期：2020-03-03 00:00:00
abstract：:The purpose of this meta-analysis was to compare the efficacy and safety of regional anesthesia to manage chronic postsurgery pain. A systematic search of PubMed, EmBase, and the Cochrane Central Register of Controlled Trials was performed to identify randomized controlled trials that focused on chronic pain frequency...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-00813-5
更新日期：2017-04-06 00:00:00
abstract：:In this study, a novel resorufin thionocarbonate-based Hg2+-selective signaling probe (RT) for microfluidic paper-based analytical device (μPAD) applications is reported. The designed probe, RT, was readily synthesized by the one-step reaction of resorufin with phenyl thionochloroformate. The RT probe displayed a prom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40169-6
更新日期：2019-03-04 00:00:00
abstract：:Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53320-0
更新日期：2019-11-19 00:00:00
abstract：:The GntR family transcription regulator HpaR1 identified from Xanthomonas campestris pv. campestris has been previously shown to positively regulate the genes responsible for hypersensitive reaction and pathogenicity and to autorepress its own expression. Here, we demonstrated that HpaR1 is a global regulator that pos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19862
更新日期：2016-01-28 00:00:00
abstract：:The modifier protein, ubiquitin (Ub) regulates various cellular pathways by controlling the fate of substrates to which it is conjugated. Ub moieties are also conjugated to each other, forming chains of various topologies. In cells, poly-Ub is attached to proteins and also exists in unanchored form. Accumulation of un...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26364-x
更新日期：2018-05-31 00:00:00
abstract：:Hepatic encephalopathy is a neuropsychiatric syndrome evolving from cerebral osmotic disturbances and oxidative/nitrosative stress. Ammonia, the main toxin of hepatic encephalopathy, triggers astrocyte senescence in an oxidative stress-dependent way. As miRNAs are critically involved in cell cycle regulation and their...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18493
更新日期：2016-01-12 00:00:00
abstract：:Angiopoietin-like protein 8 (ANGPTL8) has a role in lipid metabolism, beta-cell proliferation and diabetes progression, however, the association between different variants in the ANGPTL8 gene and metabolic syndrome (MetS) components has not been studied widely especially in Arab ethnic groups. In this study, the assoc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63850-7
更新日期：2020-04-21 00:00:00
abstract：:Similar to the onset phase of inflammation, its resolution is a process that unfolds in a manner that is coordinated and regulated by a panel of mediators. Lipoxin A4 (LXA4) has been implicated as an anti-inflammatory, pro-resolving mediator. We hypothesized that LXA4 attenuates or prevents an inflammatory response vi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27194-7
更新日期：2018-06-11 00:00:00
abstract：:Seven new formyl phloroglucinol meroterpenoids (FPMs), namely eucalrobusones J-P (1-7), as well as three known ones (8-10) were isolated from the leaves of Eucalyptus robusta. Their structures were elucidated by spectroscopic data analysis, and their absolute configurations were determined by applications of the Snatz...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39815
更新日期：2016-12-22 00:00:00
abstract：:We compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of extraction kit had ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28063
更新日期：2016-06-17 00:00:00
abstract：:Cooperation in one-shot anonymous interactions is a widely documented aspect of human behaviour. Here we shed light on the motivations behind this behaviour by experimentally exploring cooperation in a one-shot continuous-strategy Prisoner's Dilemma (i.e. one-shot two-player Public Goods Game). We examine the distribu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06790
更新日期：2014-10-28 00:00:00
abstract：:The fluorination of p-type metal phthalocyanines produces n-type semiconductors, allowing the design of organic electronic circuits that contain inexpensive heterojunctions made from chemically and thermally stable p- and n-type organic semiconductors. For the evaluation of close to intrinsic transport properties, hig...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07573
更新日期：2014-12-19 00:00:00
