Abstract:
OBJECTIVE:Osteosarcoma is a common malignant bone tumor that is frequently found in the long bones of children and adolescents. The aim of this study is to examine long noncoding RNA-steroid receptor RNA activator 1 expression in osteosarcoma to explore the biological function of long noncoding RNA steroid receptor RNA activator 1 on proliferation, migration, and invasion along with apoptosis and its regulatory mechanism, which would facilitate the early diagnosis and targeted therapy of osteosarcoma. METHODS:First, microarray analysis was applied to determine the expression of long noncoding RNAs in osteosarcoma tissues and paired normal tissues. Then, quantitative real-time polymerase chain reaction was utilized to validate microarray findings. Next, osteosarcoma cancerous cell lines SJSA-1 and U2OS were transfected with pcDNA3.1-SRA1 or pCMV-sh-SRA1 to increase or decrease steroid receptor RNA activator 1 expression levels, and microRNA-208a inhibitors, mimic to investigate the effects of microRNA-208a on osteosarcoma as well as the regulatory relation between long noncoding RNA steroid receptor RNA activator 1 and microRNA-208a. Cell proliferation was evaluated through Cell Counting Kit-8 and colony formation assays. Flow cytometry analysis was conducted to evaluate the apoptosis ratio. The migration and invasion abilities were measured using wound-healing and transwell assays. RESULTS:Long noncoding RNA-steroid receptor RNA activator 1 expression was downregulated in osteosarcoma tissues and cells compared with that in corresponding normal tissues, whereas microRNA-208a expression was upregulated in osteosarcoma tissues. Moreover, the restoration of long noncoding RNA steroid receptor RNA activator 1 inhibited cell proliferation, and upregulation of long noncoding RNA steroid receptor RNA activator 1 restrained cell migration and invasion but boosted the apoptosis rate in osteosarcoma cells. In addition, long noncoding RNA steroid receptor RNA activator 1 targeting microRNA-208a was involved in the progression of osteosarcoma. Furthermore, upregulating microRNA-208a exerted similar roles of silencing long noncoding RNA steroid receptor RNA activator 1 in cell apoptosis, proliferation, migration, and invasion, which were reversed by enhancing the expression of long noncoding RNA steroid receptor RNA activator 1. CONCLUSIONS:In our study, long noncoding RNA steroid receptor RNA activator 1 played an antitumor role in osteosarcoma as it reduced cell migration, invasion, and proliferation, but facilitated cell apoptosis via sponging microRNA-208a, which could be regarded as a potential therapeutic target of osteosarcoma treatment.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Guo W,Jiang H,Li H,Li F,Yu Q,Liu Y,Jiang W,Zhang Mdoi
10.1177/1533033819841438subject
Has Abstractpub_date
2019-01-01 00:00:00pages
1533033819841438eissn
1533-0346issn
1533-0338journal_volume
18pub_type
杂志文章abstract::We do not know the clinical and prognostic factors that influence the survival of patients with gastric signet ring cell carcinoma (SRC). Therefore, a retrospective review was undertaken of 219 patients with SRC who had undergone gastrectomy between January 2009 and December 2012 in our hospital. Patient age, sex, TNM...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820983812
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:The study was aimed to compare online 6 degree-of-freedom image registrations of TrueBeam cone-beam computed tomography and BrainLab ExacTrac X-ray imaging systems for intracranial radiosurgery. METHODS:Phantom and patient studies were performed on a Varian TrueBeam STx linear accelerator (version 2.5), which ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616683069
更新日期:2017-06-01 00:00:00
abstract::The accurate characterization and description of the vascular network of a cancer lesion is of paramount importance in clinical practice and cancer research in order to improve diagnostic accuracy or to assess the effectiveness of a treatment. The aim of this study was to show the effectiveness of liposomes as an ultr...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600272
更新日期:2014-12-01 00:00:00
abstract::Most experimental studies of electroporation focus on permeabilization of the outer cell membrane. Some experiments address delivery of ions and molecules into cells that should survive; others focus on efficient killing of the cells with minimal temperature rise. A basic method for quantifying electroporation effecti...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818792491
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abstract::The microRNA-200 (miR-200) family has been reported to be vital for the inhibition of epithelial-to-mesenchymal transition (EMT) in tumor cells. The miR-200 family represents a complex multi-factorial regulatory network which has not been well described in breast cancer. This study aimed to clarify the underlying regu...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533033820979672
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abstract::Targeted immunotherapy using dendritic cell vaccine has been employed for the treatment of solid tumors. Topical 5-aminolevulinic acid-mediated photodynamic therapy, an established approach for topical cancers, can induce an effective antitumor immune response. We have previously shown that 5-aminolevulinic acid-media...
journal_title:Technology in cancer research & treatment
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abstract::The aim of this study was to evaluate the relationship of prognosis of patients with multiple myeloma having extramedullary involvement (EMM) with the 18F- fluorodeoxyglucose(18F-FDG) maximum standardized uptake value and the expression of Ki-67 in biopsy samples. Sixty-five patients were newly diagnosed with multiple...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533033819849067
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abstract::The increasing evidence supported the role of Enhancer of Zeste Homolog 2 (EZH2) in the cancer development and progression. However, its precise role in the tumorigenesis of Nasopharyngeal Carcinoma (NPC) remains to be elucidated. EZH2 was depleted by retroviral infection in the NPC cells (HK-1, CNE-2, CNE-1 and C666-...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500315
更新日期:2013-06-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the most aggressive type of gastrointestinal tumor, with a high rate of mortality. However, identifying biomarkers for the treatment of HCC remains to be developed. We aimed to determine whether cell division cycle 25C (CDC25C) could be used as a novel diagnostic and therapeutic bioma...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533033820967474
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abstract::We describe the first histopathologic analysis of prostatic tissue following hypofractionated robotic radiation therapy. A 66 year-old man presented with stage II, low risk adenocarcinoma of the prostate and underwent elective conformal hypofractionated radiation therapy. His pretreatment evaluation revealed T1c adeno...
journal_title:Technology in cancer research & treatment
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更新日期:2010-12-01 00:00:00
abstract::Stereotactic body radiation therapy is a well-tolerated modality for the treatment of primary and metastatic liver lesions, and fiducials are often used as surrogates for tumor tracking during treatment. We evaluated respiratory-induced liver deformation by measuring the rigidity of the fiducial configuration during t...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533034616687193
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:A meta-analysis was conducted to evaluate the diagnostic performance of contrast-enhanced ultrasonography using the contrast agent SonoVue to differentiate benign from malignant breast lesions. METHOD:A comprehensive search of the literature was performed using the Embase, PubMed, and Web of Science databases ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820971583
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:To confirm the feasibility and accuracy of the method for evaluating blood supply and embolization rate of liver cancer based on I-flow software through animal experiments and clinical study. METHODS:Rabbits underwent selective angiography under different perfusion conditions in the same kidney. The blood su...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820970665
更新日期:2020-01-01 00:00:00
abstract::MicroRNAs are reported as a vital important factor in cancer cell initiation and progression processes. MicroRNA-19-3p has drawn the attention of many researchers in recent years because of its wide expression and its key role in serious kinds of tumor cells. However, the detailed mechanism of microRNA-19a-3p in these...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820917978
更新日期:2020-01-01 00:00:00
abstract::The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma ...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533034616658394
更新日期:2017-04-01 00:00:00
abstract::The aim of this study is to evaluate the volume differences between contrast-enhanced CT-based left ventricle (LV) and PET-CT-based LV and assess the impact of dose on the substructure volume differences in patients with left breast cancer treated with adjuvant radiotherapy. From October 2008 to February 2009, 14 pati...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500299
更新日期:2013-04-01 00:00:00
abstract::Electrochemotherapy is an effective antitumor treatment employing locally applied high voltage electric pulses delivered through conductive electrodes to the tumor in combination with chemotherapeutic drugs. The efficiency of electrochemotherapy strongly depends on the local electric field distribution inside the targ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460800700507
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Our objective is to explore the accuracy of magnetic resonance imaging in determining the preoperative T and N staging, pathological stage, and the length of esophageal tumor in patients with esophageal cancer. METHODS:This retrospective analysis included 57 patients admitted to the Department of Thoracic S...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819876263
更新日期:2019-01-01 00:00:00
abstract::The treatment of patients with malignant brain tumors, in particular glioblastoma multiforme (GBM) is very challenging because of their diffuse infiltrative nature and the cytological heterogeneity. The median survival of patients with newly diagnosed GBM is only 12-15 months, and only 8-12% of them survive for two ye...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460600500307
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Gene expression profiles from early-onset breast cancer and normal tissues were analyzed to explore the genes and prognostic factors associated with breast cancer. METHODS:GSE109169 and GSE89116 were obtained from the database of Gene Expression Omnibus. We firstly screened the differentially expressed gene...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820909911
更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
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更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820915710
更新日期:2020-01-01 00:00:00
abstract::Results for treating glomus jugulare tumors with radiosurgery have been limited by short follow-up and small number of patients. We report our experience using LINAC or CyberKnife in 21 tumors with a median follow-up of 66 months (Mean follow-up of 60 months). In addition, we have a subset of eight patients that were ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600507
更新日期:2007-10-01 00:00:00
abstract::Lung carcinoma is the most frequently diagnosed malignancy in the world, with the incidence increasing through the 20th century. Presentation may be as a tumor mass primarily obstructing the central bronchial lumen, or a mass infiltrating lung tissue. Cryosurgery can be used as a method of palliative treatment for bot...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460400300207
更新日期:2004-04-01 00:00:00
abstract:UNLABELLED:The purpose of this study was to compare the prognostic value of the percentage of positive biopsy cores (PPBC), the percentage of cancer volume (PCV), and the maximum involvement of biopsy cores (MIBC) as a prognostic factor in low- and intermediate-risk patients with clinically localized prostate cancer wh...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600271
更新日期:2014-06-01 00:00:00
abstract::Optical methods such as reflectance and fluorescence spectroscopy are being investigated for their potential to aid cancer detection in a quantitative, minimally invasive manner. Mathematical models of reflectance and fluorescence provide an important link between measured optical data and biomedically-relevant tissue...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.7785/tcrt.2012.500187
更新日期:2011-04-01 00:00:00
abstract::Spinal metastases are a common manifestation of malignant tumors that can cause severe pain, spinal cord compression, pathological fractures, and hypercalcemia, and these clinical manifestations will ultimately reduce the health-related quality of life and even shorten life expectancy in patient with cancer. Effective...
journal_title:Technology in cancer research & treatment
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更新日期:2020-01-01 00:00:00
abstract::Microwave imaging for medical applications has been of interest for many years. Recently, microwave imaging for breast cancer detection has gained attention due to advances in imaging algorithms, microwave hardware and computational power. The breast is relatively translucent to microwaves, accessible for imaging, and...
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pub_type: 杂志文章,评审
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更新日期:2005-02-01 00:00:00
abstract:PURPOSE:In this study, we aimed to evaluate the prognostic value of fluorodeoxyglucose uptake in the lung parenchyma and the presence of subclinical interstitial lung disease on computed tomography as predictive factors for survival following stereotactic body radiation therapy in patients with stage I non-small cell l...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818794934
更新日期:2018-01-01 00:00:00