Abstract：
INTRODUCTION:The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs. METHODS:A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs. RESULTS:The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed. DISCUSSION:This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.
journal_name
J Pharmacol Toxicol Methodsjournal_title
Journal of pharmacological and toxicological methodsauthors
Klein P,Nalos L,Dejmek J,Soukup Mdoi
10.1016/j.vascn.2019.106584subject
Has Abstractpub_date
2019-01-01 00:00:00pages
106584eissn
1056-8719issn
1873-488Xpii
S1056-8719(19)30064-4journal_volume
98pub_type
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
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journal_title：Journal of pharmacological and toxicological methods
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