当前位置： SCI文献检索 > JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS期刊下所有文献 > Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry.

Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry.

Abstract：

:A method for the rapid detection of intracellular nitric oxide (NO) generation in dissociated cerebellar granule cells using dichlorofluorescin (DCFH) and flow cytometry was developed. DCFH can be oxidized specifically by NO and this was assessed by 1) the use of SIN-1 (10 nM-100 microM), an NO donor, that induced a concentration-dependent increase in dichlorofluorescein (DCF) fluorescence and 2) the use of hemoglobin (10 microM), an NO-scavenger, that totally inhibited the increase of fluorescence induced by SIN-1 (10 microM). This assay was used to determine the ability to kainate to stimulate NO production in dissociated cerebellar granule cells. Kainate (1 microM-10 mM) induced an increase in DCF fluorescence that was partially reduced by NG-nitro-L-arginine (1 nM-10 microM), a nitric oxide synthase inhibitor (61.9% +/- 9.1), or hemoglobin (10 microM) (55.0% +/- 4.1). The method described allows evaluation of the oxidation of DCFH to produce DCF as a parameter for measuring intracellular NO generation. The extent of DCFH oxidation by NO and ROS can be determined by using NO scavengers or NO synthase inhibitors.

journal_name

J Pharmacol Toxicol Methods

journal_title

Journal of pharmacological and toxicological methods

authors

Gabriel C,Camins A,Sureda FX,Aquirre L,Escubedo E,Pallàs M,Camarasa J

doi

10.1016/s1056-8719(97)00066-x

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

93-8

issue

2

eissn

1056-8719

issn

1873-488X

pii

S1056-8719(97)00066-X

journal_volume

38

pub_type

杂志文章

相关文献

JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS文献大全
  • Troubleshooting pharmacological and toxicological methods.

    abstract：:The majority of articles published in J Pharmacol Tox Methods describe new approaches, or modifications to those established (Curtis, 2006). However, even established methods have their limitations. It is part of the practice of research to troubleshoot: to identify methodological weaknesses and correct them. This pro...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 社论

    doi：10.1016/j.vascn.2010.02.013

    authors： Curtis MJ

    更新日期：2010-03-01 00:00:00

  • A quantitative electroencephalographic method for xenobiotic screening in the canine model.

    abstract：:A method, using quantitative electroencephalography (qEEG), was developed for screening xenobiotics in conjunction with neurological examinations, for defining toxicodynamic profiles of certain drugs and chemicals in the dog. The standard 10-channel montage was used to evaluate normotonic, auditory, visual, and somato...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(94)90008-6

    authors： Jones RD,Greufe NP

    更新日期：1994-08-01 00:00:00

  • Injection of insect membrane in Xenopus oocyte: An original method for the pharmacological characterization of neonicotinoid insecticides.

    abstract：INTRODUCTION:Insect nicotinic acetylcholine receptors (nAChRs) represent a major target of insecticides, belonging to the neonicotinoid family. However, the pharmacological profile of native nAChRs is poorly documented, mainly because of a lack of knowledge of their subunit stoichiometry, their tissue distribution and ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2015.09.004

    authors： Crespin L,Legros C,List O,Tricoire-Leignel H,Mattei C

    更新日期：2016-01-01 00:00:00

  • A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy.

    abstract：INTRODUCTION:Intracellular calcium concentration ([Ca(2+)](i)) modifications in endothelial and smooth muscle cells represent a key element in the pathogenesis of cerebral artery vasospasm. Therefore, the present study documented potential application of confocal laser microscopy in the determination of contractile and...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2007.03.002

    authors： Hashimoto T,Ohata H,Nobe K,Sakai Y,Honda K

    更新日期：2007-07-01 00:00:00

  • Characterisation of a modified approach to the study of sympathetic neurotransmission and its presynaptic modulation in the isolated rabbit thoracic aorta.

    abstract：INTRODUCTION:The quantification of [(3)H]noradrenaline spillover from electrically stimulated, sympathetic nerves is a widely used method to study presynaptic effects of hormones, transmitters and drugs. Although a straightforward approach, the execution of the experiments is not trivial. This holds true mainly for a r...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(02)00170-3

    authors： Nap A,Pfaffendorf M,van Zwieten PA

    更新日期：2001-11-01 00:00:00

  • A simple method for measuring the effects of drugs on intestinal longitudinal and circular muscle.

    abstract：:A tissue preparation is described which makes it possible to examine the effect of drugs on the nerves that control both longitudinal and circular muscles of the small intestine. The preparation consists of a segment of rat ileum set up to measure the tension developed in the longitudinal muscle and intraluminal press...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(96)00110-4

    authors： Coupar IM,Liu L

    更新日期：1996-11-01 00:00:00

  • The influence of vancomycin concentration and the pH of plasma on vancomycin protein binding.

    abstract：:A review of numerous studies of the protein binding of vancomycin suggests major discrepancies among their results. The reported percent protein binding of vancomycin varies from 0% to 98%. The influence of pH and concentration on the protein binding of vancomycin was investigated in this study. There was a significan...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(92)90066-a

    authors： Chen Y,Norris RL,Schneider JJ,Ravenscroft PJ

    更新日期：1992-08-01 00:00:00

  • High-content imaging analysis for detecting the loss of drebrin clusters along dendrites in cultured hippocampal neurons.

    abstract：INTRODUCTION:Detection of drug effects on neuronal synapses is important for predicting their adverse effects. We have used drebrin as a marker to detect the synaptic changes in cultured neurons. High concentration of glutamate decreases the amount of drebrin in synapses. To increase the availability of this method for...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2019.106607

    authors： Hanamura K,Koganezawa N,Kamiyama K,Tanaka N,Oka T,Yamamura M,Sekino Y,Shirao T

    更新日期：2019-01-01 00:00:00

  • Hybridization buffer systems impact the quality of filter array data.

    abstract：INTRODUCTION:cDNA microarray technology has greatly facilitated mechanistic studies in pharmacology and toxicology. A clean hybridization with minimal background is critical for successful microarray analysis and is highly desired. However, clean hybridization alone is not enough; verification is needed. METHODS:Total...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2004.02.001

    authors： Liu J,Walker N,Waalkes MP

    更新日期：2004-07-01 00:00:00

  • The shifting landscape of safety pharmacology in 2015.

    abstract：:The relative importance of the discipline of safety pharmacology (which integrates physiology, pharmacologyand toxicology) has evolved since the incorporation of the Safety Pharmacology Society (SPS) as an entity on August 10, 2000. Safety pharmacology (SP), as a synthesis of these other fields of knowledge, is concer...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 社论

    doi：10.1016/j.vascn.2015.05.013

    authors： Pugsley MK,Authier S,Stonerook M,Curtis MJ

    更新日期：2015-09-01 00:00:00

  • Evaluation of bioimpedance spectroscopy for the measurement of body fluid compartment volumes in rats.

    abstract：INTRODUCTION:Bioimpedance spectroscopy (BIS) has been used in human and large animal research to assess body fluid compartment volumes (BFC) such as total body water (TBW), extracellular fluid volume (ECFV), and intracellular fluid volume (ICFV). To date, the application of BIS for determination of BFC in small researc...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2012.02.001

    authors： Hu L,Maslanik T,Zerebeckyj M,Plato CF

    更新日期：2012-03-01 00:00:00

  • Rat tail skin temperature monitored noninvasively by radiotelemetry: characterization by examination of vasomotor responses to thermomodulatory agents.

    abstract：INTRODUCTION:Measurement of tail skin temperature (T(sk)) of the rat can provide important information on mechanisms pertaining to physiology, pharmacology, and toxicology. T(sk) is largely under control of peripheral vascular tone, which is also sensitive to most experimental manipulations such as handling and restrai...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(02)00219-8

    authors： Gordon CJ,Puckett E,Padnos B

    更新日期：2002-03-01 00:00:00

  • Effect of multiple freeze-thaw cycles of cytoplasm samples on the activity of antioxidant enzymes.

    abstract：INTRODUCTION:In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design. METHODS:The activities of antioxidant enzymes in samples of rat liver cytoplas...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2005.03.002

    authors： Murias M,Rachtan M,Jodynis-Liebert J

    更新日期：2005-09-01 00:00:00

  • Development of a method to determine cytochrome P450 1A2, 2C9, 2D6 and 3A4 activity sheep hepatic microsomes.

    abstract：INTRODUCTION:Ex vivo studies of human fetal hepatic drug metabolism are uncommon as it requires access to functional liver tissue and therefore raises practical and ethical concerns. Large animal models provide an alternative opportunity to study changes in cytochrome P450 (CYP) activity in the mother and fetus during ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2020.106934

    authors： McBride GM,Soo JY,Varcoe T,Morrison JL,Wiese MD

    更新日期：2020-01-01 00:00:00

  • CSAHi study: Evaluation of multi-electrode array in combination with human iPS cell-derived cardiomyocytes to predict drug-induced QT prolongation and arrhythmia--effects of 7 reference compounds at 10 facilities.

    abstract：INTRODUCTION:Drug-induced QT prolongation is a major safety issue during drug development because it may lead to lethal ventricular arrhythmias. In this study, we evaluated the utility of multi-electrode arrays (MEA) with human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to predict drug-induced QT p...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vascn.2015.12.002

    authors： Kitaguchi T,Moriyama Y,Taniguchi T,Ojima A,Ando H,Uda T,Otabe K,Oguchi M,Shimizu S,Saito H,Morita M,Toratani A,Asayama M,Yamamoto W,Matsumoto E,Saji D,Ohnaka H,Tanaka K,Washio I,Miyamoto N

    更新日期：2016-03-01 00:00:00

  • A fully validated simple new method for environmental monitoring by surface sampling for cytotoxics.

    abstract：:A wipe sampling procedure followed by a simple ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for simultaneous quantification of six cytotoxic drugs: 5-fluorouracil (5FU), doxorubicin (DOXO), epirubicin (EPI), ifosfamide (IF), cyclophosphamide (CP) an...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2019.106652

    authors： Rossignol E,Amiand MB,Sorrieul J,Bard JM,Bobin-Dubigeon C

    更新日期：2020-01-01 00:00:00

  • Assessment of in vitro and in vivo recovery of gallamine using microdialysis.

    abstract：:The application of microdialysis technique for the investigation of pharmacokinetics and pharmacodynamics of drugs requires careful assessment of probe performance to ensure validity of the data obtained using this technique. The aim of this study was to establish and validate the microdialysis technique for investiga...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(00)00117-9

    authors： Sasongko L,Williams KM,Ramzan I,McLachlan AJ

    更新日期：2000-11-01 00:00:00

  • A simple method for measuring dopamine release from rat brain slices.

    abstract：:In many circumstances, rapid information is required about the effects of drugs on neurotransmitter release in brain, and a common method used is measurement of radiolabelled release from superfused brain slices or synaptosomes in vitro. However, the method requires expensive equipment and is not readily adapted to th...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/s1056-8719(97)00010-5

    authors： Thongsaard W,Kendall DA,Bennett GW,Marsden CA

    更新日期：1997-04-01 00:00:00

  • In vivo laboratory practicals in research-led teaching: an example using glucose tolerance tests in lean and obese mice.

    abstract：:The use of animal models is an essential part of medical research and drug development. The essential skills required to be able to do such research includes experimental design, statistical analysis and the actual handling and treating of the animals (in vivo skills). The number of students in the U.K. receiving trai...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2011.07.002

    authors： King AJ,Bowe JE,Sprake JA,Kinchin IM

    更新日期：2011-09-01 00:00:00

  • Altered ion transport and responsiveness to methacholine and hyperosmolarity in air interface-cultured guinea-pig tracheal epithelium.

    abstract：INTRODUCTION:Challenge of guinea-pig tracheal epithelium with hyperosmolar solution alters ion transport and evokes the release of epithelium-derived relaxing factor (EpDRF). Cultured tracheal epithelial cells (CE) offer the potential to examine biochemical pathways related to EpDRF release, but whether the bioelectric...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2006.04.005

    authors： Fedan JS,Wu DX,Van Scott MR

    更新日期：2007-03-01 00:00:00

  • Assessment of hepatobiliary function in vivo and ex vivo in the rat.

    abstract：:Many xenobiotics cause hepatobiliary toxicity and cholestasis in the rat. Initial assessment of hepatobiliary damage in rats can be accomplished by measuring serum concentrations of bile acids and bilirubin, serum activities of liver-associated enzymes such as 5'-nucleotidase, alkaline phosphatase, gamma-glutamyltrans...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,评审

    doi：10.1016/1056-8719(94)90024-8

    authors： Handler JA,Kossor DC,Goldstein RS

    更新日期：1994-02-01 00:00:00

  • Electrophysiological characterization of drug response in hSC-derived cardiomyocytes using voltage-sensitive optical platforms.

    abstract：INTRODUCTION:Voltage-sensitive optical (VSO) sensors offer a minimally invasive method to study the time course of repolarization of the cardiac action potential (AP). This Comprehensive in vitro Proarrhythmia Assay (CiPA) cross-platform study investigates protocol design and measurement variability of VSO sensors for ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2019.106612

    authors： Pfeiffer-Kaushik ER,Smith GL,Cai B,Dempsey GT,Hortigon-Vinagre MP,Zamora V,Feng S,Ingermanson R,Zhu R,Hariharan V,Nguyen C,Pierson J,Gintant GA,Tung L

    更新日期：2019-01-01 00:00:00

  • Prevention of ischemia-induced cerebral hypothermia by controlling the environmental temperature.

    abstract：:Regulation of brain temperature during and after cerebral ischemia plays an important role in the evolution of neuronal damage. Therefore, it is essential to maintain brain temperature at 37 degrees C during and after the ischemic insult. To get information about brain temperature during the experimental procedure, re...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/1056-8719(92)90016-t

    authors： Seif el Nasr M,Nuglisch J,Krieglstein J

    更新日期：1992-03-01 00:00:00

  • The development and testing of a dermal exposure system for pharmacokinetic studies of administered and ambient water contaminants.

    abstract：INTRODUCTION:In order to investigate the pharmacokinetics of water-borne chemicals while eliminating exposures by other routes, a dermal exposure system was developed to expose the hand and forearm of human subjects. METHODS:The goal was, primarily, to study the dermal pharmacokinetics of methyl tertiary butyl ether (...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/S1056-8719(03)00004-2

    authors： Prah JD,Blount B,Cardinali FL,Ashley DL,Leavens T,Case MW

    更新日期：2002-05-01 00:00:00

  • Isolated heart perfusion according to Langendorff---still viable in the new millennium.

    abstract：:The isolated perfused mammalian heart preparation was established in 1897 by Oscar Langendorff. The method was developed on the basis of the isolated perfused frog heart established by Elias Cyon at the Carl Ludwig Institute of Physiology in Leipzig, Germany in 1866. Observations made using both methods at the end of ...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.vascn.2006.05.006

    authors： Skrzypiec-Spring M,Grotthus B,Szelag A,Schulz R

    更新日期：2007-03-01 00:00:00

  • Adaptation to a blood pressure telemetry system revealed by measures of activity, agility and operant learning in mice.

    abstract：INTRODUCTION:Implantable telemetry enables continuous monitoring of physiological functions in freely moving animals and can greatly complement pharmacological research. Despite its miniaturization, a sensor/transmitter constitutes 5% or more of a mouse's bodyweight. The aim of the present study was to evaluate whether...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2017.02.002

    authors： Popova A,Tsvirkun D,Dolgov O,Anokhin K,Alberts J,Lagereva E,Custaud MA,Gauquelin-Koch G,Vinogradova O,Andreev-Andrievskiy A

    更新日期：2017-01-01 00:00:00

  • Rhesus monkey model for concurrent analyses of in vivo selectivity, pharmacokinetics and pharmacodynamics of aldosterone synthase inhibitors.

    abstract：INTRODUCTION:In vivo profiles of aldosterone synthase inhibitors (ASIs) have been investigated utilizing various rodent models. Due to lack of CYP17 activity, rodents produce corticosterone rather than cortisol as that of humans, which raised concern to their effectiveness in translational pharmacological characterizat...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2014.09.011

    authors： Cai TQ,Stribling S,Tong X,Xu L,Wisniewski T,Fontenot JA,Struthers M,Akinsanya KO

    更新日期：2015-01-01 00:00:00

  • A novel approach to data processing of the QT interval response in the conscious telemetered beagle dog.

    abstract：INTRODUCTION:Drug-induced QT interval prolongation may lead to ventricular arrhythmias. The aim of the study was to optimize QT interval data processing to quantify drug-induced QT interval prolongation in the telemetry instrumented conscious dog model. METHODS:The test substances cisapride, dofetilide, haloperidol, a...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2006.02.009

    authors： Ollerstam A,Persson AH,Visser SA,Fredriksson JM,Forsberg T,Nilsson LB,Eklund G,Wiklund SJ,Gabrielsson J,Duker G,Al-Saffar A

    更新日期：2007-01-01 00:00:00

  • A framework to assess the translation of safety pharmacology data to humans.

    abstract：:This article outlines a strategy for collecting accurate data for the determination of the sensitivity, specificity and predictive value of safety pharmacology models. This entails performing a retrospective analysis on commonly used safety pharmacology endpoints and an objective assessment of new non-clinical models....

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章

    doi：10.1016/j.vascn.2009.05.011

    authors： Valentin JP,Bialecki R,Ewart L,Hammond T,Leishmann D,Lindgren S,Martinez V,Pollard C,Redfern W,Wallis R

    更新日期：2009-09-01 00:00:00

  • Objective markers of the analgesic response to morphine in experimental pain research.

    abstract：INTRODUCTION:In experimental pain research the effect of opioids is normally assessed by verbal subjective response to analgesia. However, as many confounders in pain assessment exist, objective bed-side assessment of the effect is highly warranted. Therefore, we aimed to assess the effect of morphine on three objectiv...

    journal_title：Journal of pharmacological and toxicological methods

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vascn.2015.01.005

    authors： Brokjær A,Olesen AE,Kreilgaard M,Graversen C,Gram M,Christrup LL,Dahan A,Drewes AM

    更新日期：2015-05-01 00:00:00