Isolated heart perfusion according to Langendorff---still viable in the new millennium.

Abstract:

:The isolated perfused mammalian heart preparation was established in 1897 by Oscar Langendorff. The method was developed on the basis of the isolated perfused frog heart established by Elias Cyon at the Carl Ludwig Institute of Physiology in Leipzig, Germany in 1866. Observations made using both methods at the end of the 19th and at the beginning of the 20th century led to important discoveries, forming the basis for our understanding of heart physiology. This included the role of temperature, oxygen and calcium ions for heart contractile function, the origin of cardiac electrical activity in the atrium, the negative chronotropic effect of vagus stimulation and the chemical transmission of impulses in the vagus nerve by acetylcholine. Langendorff himself demonstrated that the heart receives its nutrients and oxygen from blood via the coronary arteries and that cardiac mechanical function is reflected by changes in the coronary circulation. The method underwent many modifications but its general principle remains the same today. Blood, or more commonly crystalloid perfusates, are delivered into the heart through a cannula inserted in the ascending aorta, either at constant pressure or constant flow. Retrograde flow in the aorta closes the leaflets of the aortic valve and as a consequence, the entire perfusate enters the coronary arteries via the ostia at the aortic root. After passing through the coronary circulation the perfusate drains into the right atrium via the coronary sinus. The simplicity of the isolated mammalian heart preparation, the broad spectrum of measurements which can be done using this method, its high reproducibility and relatively low cost make it a very useful tool in modern cardiovascular and pharmacological research, in spite of a few shortcomings. In the last decade the method has brought many important advances in many areas including ischemia-reperfusion injury, cell-based therapy and donor heart preservation for transplant.

authors

Skrzypiec-Spring M,Grotthus B,Szelag A,Schulz R

doi

10.1016/j.vascn.2006.05.006

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

113-26

issue

2

eissn

1056-8719

issn

1873-488X

pii

S1056-8719(06)00066-9

journal_volume

55

pub_type

历史文章,杂志文章,评审
  • Comparison of the QT interval response during sinus and paced rhythm in conscious and anesthetized beagle dogs.

    abstract:INTRODUCTION:The aim of the present study was to compare sensitivity in detecting the drug-induced QT interval prolongation in three dog models: conscious telemetered at sinus rhythm and conscious and anesthetized dogs during atrial pacing. The test substances used represent different chemical classes with different ph...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2007.05.002

    authors: Ollerstam A,Visser SA,Duker G,Forsberg T,Persson AH,Nilsson LB,Björkman JA,Gabrielsson J,Al-Saffar A

    更新日期:2007-09-01 00:00:00

  • A comparison of rectal and subcutaneous body temperature measurement in the common marmoset.

    abstract::Two methods of measuring body temperature were compared in common marmosets. Subcutaneous temperatures were measured remotely via previously implanted subcutaneous microchips (Plexx BV, IPTT-100) prior to measurement of rectal temperature using a conventional rectal probe. Marmosets were treated with saline or the bra...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(98)00030-6

    authors: Cilia J,Piper DC,Upton N,Hagan JJ

    更新日期:1998-07-01 00:00:00

  • Influence of tissue freezing on the binding of 125I-sarcosine1, isoleucine8 angiotensin II to angiotensin II receptor subtypes in the rat.

    abstract::Evaluation of angiotensin II (AII) receptor binding often necessitates freezing of the tissue of interest prior to assay of radioligand binding. This study evaluated the effects of freezing of various rat tissues at different rates on 125I-sarcosine1, isoleucine8 angiotensin II (125I-SI AII) binding to AII receptor su...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/1056-8719(94)00061-8

    authors: Lu XY,Zhang W,Grove KL,Speth RC

    更新日期:1995-04-01 00:00:00

  • Pharmacologic action of Escherichia coli heat-stable (STa) enterotoxin.

    abstract::Escherichia coli produces a heat-stable (STa) enterotoxin that belongs to a family of peptides that mediate several diarrheal diseases, including traveler's diarrhea and epidemic diarrhea in infants and newborns. The STa enterotoxin consists of 18 or 19 amino acids and is encoded by genes specified on a transposon. In...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章,评审

    doi:10.1016/1056-8719(92)90049-7

    authors: Knoop FC,Owens M

    更新日期:1992-09-01 00:00:00

  • Effect of multiple freeze-thaw cycles of cytoplasm samples on the activity of antioxidant enzymes.

    abstract:INTRODUCTION:In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design. METHODS:The activities of antioxidant enzymes in samples of rat liver cytoplas...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2005.03.002

    authors: Murias M,Rachtan M,Jodynis-Liebert J

    更新日期:2005-09-01 00:00:00

  • Characterization of a refinement of the "pylorus ligation" model of rat gastric ulceration resulting in "no pain" and a more specific pharmacological response.

    abstract:INTRODUCTION:The pharmacological assessment of the factors for gastric protection of a test substance should involve experimental models that can determine the involvement of cytoprotective factors, as well as their influence on the secretion of hydrochloric acid. The original protocol of pylorus ligation in rats propo...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2012.09.002

    authors: Monteiro KM,Spindola HM,Possenti A,Tinti SV,Ruiz AL,Longato GB,Fiorito GF,Marchetti GM,Shiozawa L,Piloni BU,de Oliveira AC,Miyagawa LM,Carvalho JE

    更新日期:2013-03-01 00:00:00

  • Successful in vitro growth of rat two-cell embryos to blastocysts using a simple chemically defined medium.

    abstract::The aim of the present study was to formulate a simple chemically defined medium for the in vitro growth of rat two-cell embryos to blastocysts. Embryos from day 2 pregnant rats were retrieved and placed in paraffin oil-covered droplets of "rat two-cell embryo culture medium" (R2ECM) containing combinations of various...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(00)00084-8

    authors: Goh VH,Adiga SK,Tain CF,Tong TY,Ng SC

    更新日期:2000-05-01 00:00:00

  • A quantitative electroencephalographic method for xenobiotic screening in the canine model.

    abstract::A method, using quantitative electroencephalography (qEEG), was developed for screening xenobiotics in conjunction with neurological examinations, for defining toxicodynamic profiles of certain drugs and chemicals in the dog. The standard 10-channel montage was used to evaluate normotonic, auditory, visual, and somato...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/1056-8719(94)90008-6

    authors: Jones RD,Greufe NP

    更新日期:1994-08-01 00:00:00

  • Isolation of liver nuclei that retain functional trans-membrane transport.

    abstract::We have developed a method for the rapid isolation of hepatocyte nuclei, which employs gentle homogenization and centrifugation conditions, and involves minimal processing time. The purified nuclei were morphologically unaltered when observed by light and electron microscopy. No significant contamination from cytoplas...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(97)00082-8

    authors: Ho YF,Guenthner TM

    更新日期:1997-11-01 00:00:00

  • Insights into toxicology, safety pharmacology, and drug dependence testing: The performance and predictive values of nonclinical tests.

    abstract::The pre-marketing testing of drugs and chemicals is now a paradigm of contribution to the safeguard of public health. As pointed out by the ICH concept paper E14/S7B, there is a need for science-based frameworks that allow a better design, conduction, and interpretation of nonclinical and toxicity tests, particularly,...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2020.106684

    authors: Suarez-Torres JD,Ciangherotti CE,Jimenez-Orozco FA

    更新日期:2020-01-01 00:00:00

  • The shifting landscape of safety pharmacology in 2015.

    abstract::The relative importance of the discipline of safety pharmacology (which integrates physiology, pharmacologyand toxicology) has evolved since the incorporation of the Safety Pharmacology Society (SPS) as an entity on August 10, 2000. Safety pharmacology (SP), as a synthesis of these other fields of knowledge, is concer...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 社论

    doi:10.1016/j.vascn.2015.05.013

    authors: Pugsley MK,Authier S,Stonerook M,Curtis MJ

    更新日期:2015-09-01 00:00:00

  • A reliable procedure for comparison of antioxidants in rat brain homogenates.

    abstract::Lipid peroxidation is a major consequence of oxidative stress and an important cause of neuronal damage in ischaemic injuries and neurodegenerative disorders such as Parkinson's disease. Recent research has focused on the development of antioxidant drugs which may delay or minimize neurodegeneration. Rapid and reliabl...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(98)00022-7

    authors: Callaway JK,Beart PM,Jarrott B

    更新日期:1998-04-01 00:00:00

  • Safety pharmacology in 2014: new focus on non-cardiac methods and models.

    abstract::"What do you know about Safety Pharmacology?" This is the question that was asked in 2000 with the inception of the Safety Pharmacology Society (SPS). There is now a widespread awareness of the role of safety pharmacology in drug discovery and increasing awareness among the wider community of methods and models used i...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type:

    doi:10.1016/j.vascn.2014.08.004

    authors: Pugsley MK,Dalton JA,Authier S,Curtis MJ

    更新日期:2014-09-01 00:00:00

  • ADVAN-style analytical solutions for common pharmacokinetic models.

    abstract:INTRODUCTION:The analytical solutions to compartmental pharmacokinetic models are well known, but have not been presented in a form that easily allows for complex dosing regimen and changes in covariate/parameter values that may occur at discrete times within and/or between dosing intervals. METHODS:Laplace transforms...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2015.03.004

    authors: Abuhelwa AY,Foster DJ,Upton RN

    更新日期:2015-05-01 00:00:00

  • Progress in computational toxicology.

    abstract:INTRODUCTION:Computational methods have been widely applied to toxicology across pharmaceutical, consumer product and environmental fields over the past decade. Progress in computational toxicology is now reviewed. METHODS:A literature review was performed on computational models for hepatotoxicity (e.g. for drug-indu...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.vascn.2013.12.003

    authors: Ekins S

    更新日期:2014-03-01 00:00:00

  • An animal model for the assessment of gingival lesions.

    abstract::A model of gingival inflammation was performed in Sprague-Dawley rats weighing 180-200 g. Mechanical bamboo stick-induced injury was inflammatory when bacteria contaminated the sticks. Bacteria were first obtained from gingival fluid collected from a patient with adult periodontitis. Another strain from Institut Paste...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/1056-8719(94)90070-1

    authors: Guessous F,Huynh C,N'Guyen H,Godeau G,Giroud JP,Meyer J,Hornebeck W,Roch-Arveiller M

    更新日期:1994-11-01 00:00:00

  • Assessment of hepatobiliary function in vivo and ex vivo in the rat.

    abstract::Many xenobiotics cause hepatobiliary toxicity and cholestasis in the rat. Initial assessment of hepatobiliary damage in rats can be accomplished by measuring serum concentrations of bile acids and bilirubin, serum activities of liver-associated enzymes such as 5'-nucleotidase, alkaline phosphatase, gamma-glutamyltrans...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章,评审

    doi:10.1016/1056-8719(94)90024-8

    authors: Handler JA,Kossor DC,Goldstein RS

    更新日期:1994-02-01 00:00:00

  • The influence of vancomycin concentration and the pH of plasma on vancomycin protein binding.

    abstract::A review of numerous studies of the protein binding of vancomycin suggests major discrepancies among their results. The reported percent protein binding of vancomycin varies from 0% to 98%. The influence of pH and concentration on the protein binding of vancomycin was investigated in this study. There was a significan...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/1056-8719(92)90066-a

    authors: Chen Y,Norris RL,Schneider JJ,Ravenscroft PJ

    更新日期:1992-08-01 00:00:00

  • Functional properties and substrate characterization of human CYP26A1, CYP26B1, and CYP26C1 expressed by recombinant baculovirus in insect cells.

    abstract:INTRODUCTION:The cytochrome P450 CYP26 family of retinoic acid (RA) metabolizing enzymes, comprising CYP26A1, CYP26B1, and CYP26C1 is critical for establishing patterns of RA distribution during embryonic development and retinoid homeostasis in the adult. All three members of this family can metabolize all trans-RA. CY...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2011.08.005

    authors: Helvig C,Taimi M,Cameron D,Jones G,Petkovich M

    更新日期:2011-11-01 00:00:00

  • The use of small interfering RNA to elucidate the activity and function of ion channel genes in an intact tissue.

    abstract::Small interfering RNA (siRNA) directs the targeted destruction of mRNA encoding a specific protein, in a process known as RNA interference (RNAi). This stops translation of the targeted mRNA into protein, effectively silencing the gene. RNAi is a recent discovery, identified in mammalian cells in 2001, but it has rapi...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2004.08.012

    authors: Gurney AM,Hunter E

    更新日期:2005-05-01 00:00:00

  • Gas chromatography-mass spectrometry assay for determination of ketamine in brain.

    abstract::A sensitive and precise gas chromatography-mass spectrometry method with selected ion monitoring has been developed for determination of ketamine in the brain using chlorpheniramine as an internal standard. The assay is based on the acid extraction of brain homogenate with hexane and ethyl ether with subsequent alkali...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(99)00023-4

    authors: Blednov YA,Simpson VJ

    更新日期:1999-04-01 00:00:00

  • A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy.

    abstract:INTRODUCTION:Intracellular calcium concentration ([Ca(2+)](i)) modifications in endothelial and smooth muscle cells represent a key element in the pathogenesis of cerebral artery vasospasm. Therefore, the present study documented potential application of confocal laser microscopy in the determination of contractile and...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2007.03.002

    authors: Hashimoto T,Ohata H,Nobe K,Sakai Y,Honda K

    更新日期:2007-07-01 00:00:00

  • Antibodies to a synthetic peptide that react with flavin-containing monooxygenase (HLFMO3) in human hepatic microsomes.

    abstract::Flavin-containing monooxygenases (FMOs) catalyze the oxidation of a diverse array of xenobiotic compounds. The purpose of this investigation was to develop a specific immunological probe to human hepatic flavin-containing monooxygenase (HLFMO3). An oligopetide corresponding to amino acid residues 257-270 of HLFMO3 was...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(97)00012-9

    authors: Myers CR,Porgilsson B,Myers JM

    更新日期:1997-03-01 00:00:00

  • In vivo laboratory practicals in research-led teaching: an example using glucose tolerance tests in lean and obese mice.

    abstract::The use of animal models is an essential part of medical research and drug development. The essential skills required to be able to do such research includes experimental design, statistical analysis and the actual handling and treating of the animals (in vivo skills). The number of students in the U.K. receiving trai...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2011.07.002

    authors: King AJ,Bowe JE,Sprake JA,Kinchin IM

    更新日期:2011-09-01 00:00:00

  • Evidence for a peripheral mechanism of action for the potentiation of the antinociceptive effect of morphine by dipyrone.

    abstract::The potentiation of the antinociceptive effect of morphine by dipyrone (metamizol) and the possible participation of a peripheral mechanism on such synergism were studied with the use of the formalin test in the rat. Nociception was induced by the intraplantar injection of diluted formalin (1%) in the right hind paw. ...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/s1056-8719(00)00046-0

    authors: Aguirre-Bañuelos P,Granados-Soto V

    更新日期:1999-10-01 00:00:00

  • Troubleshooting pharmacological and toxicological methods.

    abstract::The majority of articles published in J Pharmacol Tox Methods describe new approaches, or modifications to those established (Curtis, 2006). However, even established methods have their limitations. It is part of the practice of research to troubleshoot: to identify methodological weaknesses and correct them. This pro...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 社论

    doi:10.1016/j.vascn.2010.02.013

    authors: Curtis MJ

    更新日期:2010-03-01 00:00:00

  • Sensitivity and specificity of isolated perfused guinea pig heart to test for drug-induced lengthening of QTc.

    abstract:INTRODUCTION:The purpose of this study was to determine the sensitivity and specificity for predicting the liability of a compound to lengthen QTc using isolated, perfused guinea pig hearts (Langendorff preparation). METHODS:QTc (Fridericia correction) was calculated from bipolar transventricular electrograms. Hearts ...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2003.08.003

    authors: Hamlin RL,Cruze CA,Mittelstadt SW,Kijtawornrat A,Keene BW,Roche BM,Nakayama T,Nakayama H,Hamlin DM,Arnold T

    更新日期:2004-01-01 00:00:00

  • ILSI-HESI cardiovascular safety subcommittee dataset: an analysis of the statistical properties of QT interval and rate-corrected QT interval (QTc).

    abstract:INTRODUCTION:The Health and Environmental Sciences Institute of the International Life Sciences Institute (ILSI/HESI) Cardiovascular Safety Subcommittee outlined a set of in vivo telemetry studies to determine how well this preclinical model identified compounds known to cause torsades de pointes (TdP) and prolong QT i...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2007.04.002

    authors: Chiang AY,Bass AS,Cooper MM,Engwall MJ,Menton RG,Thomas K

    更新日期:2007-09-01 00:00:00

  • A simple, automatic method for morphometric analysis of the left ventricle in rats with myocardial infarction.

    abstract::Induction of acute myocardial infarction in the rat is an established model for studying effects of therapeutic interventions. Images of sections of the rat left ventricle, stained with nitroblue tetrazolium, were digitized and several parameters estimated by dedicated software on an image analyzer (IBAS 2.0). The met...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/1056-8719(95)00019-e

    authors: Porzio S,Masseroli M,Messori A,Forloni G,Olivetti G,Jeremic G,Riva E,Luvarà G,Latini R

    更新日期:1995-08-01 00:00:00

  • Monophasic action potential in anaesthetized guinea pigs as a biomarker for prediction of liability for drug-induced delayed ventricular repolarization.

    abstract:INTRODUCTION:Drug-induced QT interval prolongation has been one of the critical issues for developing new chemical entities and pharmaceutical companies need to evaluate the risk early in the development stage. At such stage, guinea pigs are appropriate due to their small size requiring only small amounts of test drugs...

    journal_title:Journal of pharmacological and toxicological methods

    pub_type: 杂志文章

    doi:10.1016/j.vascn.2006.11.002

    authors: Tabo M,Kimura K,Ito S

    更新日期:2007-05-01 00:00:00