当前位置： SCI文献检索 > JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS期刊下所有文献 > A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy.

A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy.

Abstract：

INTRODUCTION:Intracellular calcium concentration ([Ca(2+)](i)) modifications in endothelial and smooth muscle cells represent a key element in the pathogenesis of cerebral artery vasospasm. Therefore, the present study documented potential application of confocal laser microscopy in the determination of contractile and [Ca(2+)](i) responses in basilar artery. METHODS:Experiments were performed on the rat isolated basilar artery. Changes in [Ca(2+)](i) were determined by ratiometry involving Fluo-4/AM and Fura Red/AM. Contractile function was calculated from the change in fluorescent area by Fluo-4/AM. RESULTS:KCl (50 mM) elicited an increase in [Ca(2+)](i) and contraction in basilar artery; moreover, nearly well maintained responses were evident for at least 120 min following the first application. 10 microM 5-hydroxytryptamine (5-HT), 10 microM alpha,beta-methyleneadenosine 5'-triphosphate (alpha,beta-meATP) and 10 nM vasopressin (VP) also induced increases in [Ca(2+)](i) and contraction dose-dependently. Additionally, 10 microM acetylcholine elicited a transient [Ca(2+)](i) decrease and sustained relaxation. In individual cells, rhythmical changes in [Ca(2+)](i) were observed after 10 microM 5-HT. VP (10 nM) evoked modest Ca(2+) oscillation in individual cells; however, Ca(2+) oscillation was not detectable with 10 microM alpha,beta-meATP. DISCUSSION:These results indicate that this method offers reproducibility and quantifiable effects. Imaging technology may therefore be applied to the estimation of [Ca(2+)](i) responses at the tissue level as well as at the level of the individual cell. Thus, confocal laser microscopy is a suitable tool for estimation of small artery function.

journal_name

J Pharmacol Toxicol Methods

journal_title

Journal of pharmacological and toxicological methods

authors

Hashimoto T,Ohata H,Nobe K,Sakai Y,Honda K

doi

10.1016/j.vascn.2007.03.002

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

79-86

issue

eissn

1056-8719

issn

1873-488X

pii

S1056-8719(07)00181-5

journal_volume

pub_type

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A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy.