当前位置： SCI文献检索 > Experimental and Therapeutic Medicine期刊下所有文献 > Vascular repair and anti-inflammatory effects of soluble epoxide hydrolase inhibitor.

Vascular repair and anti-inflammatory effects of soluble epoxide hydrolase inhibitor.

Abstract：

:Kawasaki disease (KD) is the leading cause of acquired heart disease in pediatric patients in developed countries. Coronary artery aneurysms and myocardial infarction may occur if the disease remains untreated. An estimated 10-20% of KD patients do not respond to intravenous gamma globulin (IVIG), and thus, alternative treatments are currently being investigated. Epoxyeicosatrienoic acids (EETs) are natural anti-inflammatory factors and angiogenic mediators degraded by soluble epoxide hydrolase (sEH). sEH inhibitory factors have been demonstrated to stabilize EET levels, inhibit inflammation and promote vascular repair in vivo. The present study aimed to determine whether an increase in EET levels induced by treatment with the sEH inhibitor 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA) promotes vascular repair in human coronary arterial endothelial cells (HCAECs) and reduces inflammation in a mouse model of KD induced by Lactobacillus casei cell wall extract. The effect of AUDA on vascular repair in HCAECs was assessed by using cell proliferation, migration, adhesion and tube formation assays, and the anti-inflammatory effect of AUDA in the mouse model of KD was determined by detecting the expression of matrix metalloproteinase (MMP)-9, tumor necrosis factor (TNF)-α and interleukin (IL)-1β at the protein level via ELISA. The results demonstrated that AUDA increased the proliferation, migration, adhesion and tube formation ability of HCAECs in a dose-dependent manner. Furthermore, in the mouse model of KD, AUDA reduced the protein expression of MMP-9, IL-1β and TNF-α, indicating that AUDA may alleviate inflammatory reactions in the coronary arteries of KD model mice. The present results also indicate that these effects may be exerted through the peroxisome proliferator activated receptor γ signaling pathway. Taken together, the present study supports the potential utility of AUDA in the treatment of KD.

journal_name

Exp Ther Med

journal_title

Experimental and therapeutic medicine

authors

Dai N,Zhao C,Kong Q,Li D,Cai Z,Wang M

doi

10.3892/etm.2019.7396

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

3580-3588

issue

eissn

1792-0981

issn

1792-1015

pii

ETM-0-0-7396

journal_volume

pub_type

杂志文章

在线工具

Vascular repair and anti-inflammatory effects of soluble epoxide hydrolase inhibitor.