Abstract:
:The association between long non-coding RNA zinc finger antisense 1 (ZFAS1) and acute myeloid leukemia (AML) has not yet been investigated. The present study aimed to assess the potential role of ZFAS1 in AML cell proliferation and apoptosis. The expression of ZFAS1 mRNA in various AML cell lines (HL-60, KG-1, ML-1 and SKNO-1) was measured by reverse transcription-quantitative polymerase chain reaction. The results showed that ZFAS1 expression was increased in all four human AML cell lines compared with the control cell lines (T lymphocytic leukemia or Burkitt's lymphoma). Transfection with small interfering RNA into human AML cells established ZFAS1 knockdown. A cell-counting kit-8 (CCK-8) assay was used to investigate the effect of ZFAS1 on AML cell proliferation and the effect of ZFAS1 on the cell cycle and cell apoptosis was assessed using flow cytometry. Notably, the CCK-8 assay demonstrated that ZFAS1 knockdown inhibited cell proliferation in HL-60 and SKNO-1 cell lines and flow cytometry analysis indicated that ZFAS1 knockdown induced AML cell cycle G1 phase arrest and triggered cell apoptosis. Therefore, the present study indicated that ZFAS1 promoted the proliferation and inhibited the apoptosis of AML cells.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Guo H,Wu L,Zhao P,Feng Adoi
10.3892/etm.2017.4535subject
Has Abstractpub_date
2017-07-01 00:00:00pages
647-651issue
1eissn
1792-0981issn
1792-1015pii
ETM-0-0-4535journal_volume
14pub_type
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