Establishment of a dual mode immunochromatographic assay for Campylobacter jejuni detection.

Abstract:

:Campylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis. Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS) enhancement properties of platinum coated gold nanorods (AuNR@Pt), was developed in this study. Under color mode and SERS mode, the proposed assay showed good linear response in the range of 102-106 cfu/mL and 102-5 × 106 cfu/mL with limits of detection of 75 cfu/mL and 50 cfu/mL, respectively (S/N = 3). Furthermore, the reliability of the dual-readout lateral flow assay (LFA) was successfully demonstrated by the application on milk samples, in which the recoveries ranged from 89.33% to 107.62%. Overall, the immunochromatographic assay developed in this work is promising and has good chance to be employed for sensitive detection of C. jejuni in food products.

journal_name

Food Chem

journal_title

Food chemistry

authors

He D,Wu Z,Cui B,Xu E,Jin Z

doi

10.1016/j.foodchem.2019.03.106

subject

Has Abstract

pub_date

2019-08-15 00:00:00

pages

708-713

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(19)30592-8

journal_volume

289

pub_type

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