Abstract:
:Campylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis. Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS) enhancement properties of platinum coated gold nanorods (AuNR@Pt), was developed in this study. Under color mode and SERS mode, the proposed assay showed good linear response in the range of 102-106 cfu/mL and 102-5 × 106 cfu/mL with limits of detection of 75 cfu/mL and 50 cfu/mL, respectively (S/N = 3). Furthermore, the reliability of the dual-readout lateral flow assay (LFA) was successfully demonstrated by the application on milk samples, in which the recoveries ranged from 89.33% to 107.62%. Overall, the immunochromatographic assay developed in this work is promising and has good chance to be employed for sensitive detection of C. jejuni in food products.
journal_name
Food Chemjournal_title
Food chemistryauthors
He D,Wu Z,Cui B,Xu E,Jin Zdoi
10.1016/j.foodchem.2019.03.106subject
Has Abstractpub_date
2019-08-15 00:00:00pages
708-713eissn
0308-8146issn
1873-7072pii
S0308-8146(19)30592-8journal_volume
289pub_type
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