Abstract:
:The increasing global trade of fishery and aquaculture products makes it necessary to develop methods for species identification in case of fish fillets or other highly processed seafood with external morphological characteristics (e.g. gills, fins) of the original fish being removed. Species identification methods based on DNA- or protein-analysis, like DNA sequencing of PCR products, PCR-SSCP (polymerase chain reaction - single strand conformation polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of fish fillet were applied successfully to distinguish between various sparid species including Sparus aurata, Spondyliosoma cantharus, Boops boops, Acanthopagrus bifasciatus, Argyrops spinifer, Pagellus bogaraveo and Lithognathus mormyrus of the family Sparidae available on European markets. Indication of the presence of parvalbumins in light muscle of all of the studied species is presented by the location of heat-stable proteins in the anodic range of the IEF gels.
journal_name
Food Chemjournal_title
Food chemistryauthors
Schiefenhövel K,Rehbein Hdoi
10.1016/j.foodchem.2012.10.057subject
Has Abstractpub_date
2013-05-01 00:00:00pages
154-60issue
1eissn
0308-8146issn
1873-7072pii
S0308-8146(12)01595-6journal_volume
138pub_type
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