Optimization of ascitic fluid culture technique.

Abstract:

:The conventional method of ascitic fluid culture detects bacteria in only 42%-65% of patients who have neutrocytic ascites and suspected spontaneous bacterial peritonitis. In this study ascitic fluid was cultured by the conventional method as well as by a new method consisting of bedside inoculation of blood culture bottles with ascites. The conventional cultures grew bacteria in only 13 (43%) of 30 episodes of neutrocytic ascites, whereas the blood culture bottles grew bacteria in 28 (93%); this difference was significant (p less than 0.0001). The blood culture bottle method also resulted in more rapid detection of bacterial growth. The median concentration of bacteria in infected ascites was one organism per milliliter. Bedside inoculation of blood culture bottles with ascitic fluid is more sensitive than the conventional method in detecting bacterial peritonitis. The insensitivity of the conventional method is probably due to the low concentration of bacteria in infected ascites and the small volume of ascites cultured by this method.

journal_name

Gastroenterology

journal_title

Gastroenterology

authors

Runyon BA,Canawati HN,Akriviadis EA

doi

10.1016/0016-5085(88)90372-1

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

1351-5

issue

5

eissn

0016-5085

issn

1528-0012

pii

0016-5085(88)90372-1

journal_volume

95

pub_type

杂志文章
  • Immunohistochemical study of epithelial cell proliferation in hyperplastic polyps, adenomas, and adenocarcinomas of the large bowel.

    abstract::A monoclonal antibody to bromodeoxyuridine was used in tissue specimens previously incubated with bromodeoxyuridine to show S-phase cells by immunohistochemical technique. Biopsy specimens of normal mucosa (n = 10), hyperplastic polyps (n = 10), adenomas with low-grade dysplasia (n = 20), adenomas with high-grade dysp...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(88)90545-8

    authors: Risio M,Coverlizza S,Ferrari A,Candelaresi GL,Rossini FP

    更新日期:1988-04-01 00:00:00

  • Human intestinal motor activity and transport: effects of a synthetic opiate.

    abstract::Effects of opiates on intestinal motor activity and transport of water and electrolytes have been studied separately in previous investigations. The aim of these experiments was to evaluate simultaneously the effects of a synthetic opiate, loperamide, on motor activity and transport in the human intestine. Jejunal, il...

    journal_title:Gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.1016/0016-5085(86)90079-x

    authors: Kachel G,Ruppin H,Hagel J,Barina W,Meinhardt M,Domschke W

    更新日期:1986-01-01 00:00:00

  • High levels of hepatitis B surface antigen increase risk of hepatocellular carcinoma in patients with low HBV load.

    abstract:BACKGROUND & AIMS:Patients with chronic hepatitis B virus (HBV) infection have a high risk for developing hepatocellular carcinoma (HCC). Patients with lower levels of hepatitis B surface antigen (HBsAg) have higher chances of losing HBsAg than those with high levels. However, little is known about whether higher level...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2012.02.007

    authors: Tseng TC,Liu CJ,Yang HC,Su TH,Wang CC,Chen CL,Kuo SF,Liu CH,Chen PJ,Chen DS,Kao JH

    更新日期:2012-05-01 00:00:00

  • Comparative nutritional analysis of chemically defined diets.

    abstract::Low residue, chemically defined diets containing all essential nutrients in a readily assimilated form, require minimal digestion and are almost entirely absorbed in the upper gastrointestinal tract. Within the past decade, these diets have been reported to provide nutritional support in a variety of clinical situatio...

    journal_title:Gastroenterology

    pub_type: 杂志文章,评审

    doi:

    authors: Young EA,Heuler N,Russell P,Weser E

    更新日期:1975-12-01 00:00:00

  • Compositional and functional features of the gastrointestinal microbiome and their effects on human health.

    abstract::The human gastrointestinal tract contains distinct microbial communities that differ in composition and function based on their location, as well as age, sex, race/ethnicity, and diet of their host. We describe the bacterial taxa present in different locations of the GI tract, and their specific metabolic features. Th...

    journal_title:Gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1053/j.gastro.2014.01.052

    authors: Hollister EB,Gao C,Versalovic J

    更新日期:2014-05-01 00:00:00

  • Dying a thousand deaths: redundant pathways from different organelles to apoptosis and necrosis.

    abstract::Cell death is an essential event in normal life and development, as well as in the pathophysiological processes that lead to disease. Although the literature on cell death has grown enormously in size and complexity, a pattern has emerged that each of several distinct organelles (plasma membrane, mitochondrion, nucleu...

    journal_title:Gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1053/j.gastro.2005.06.006

    authors: Lemasters JJ

    更新日期:2005-07-01 00:00:00

  • Guaran effect on rat intestinal absorption. A perfusion study.

    abstract::Among the components of dietary fiber, the soluble polysaccharides, primarily guaran and pectin, have been found to impair intestinal absorption. Little is known, however, about the mechanism of this effect. The direct action of guaran on small intestinal absorption was evaluated by a single-pass perfusion technique. ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Elsenhans B,Zenker D,Caspary WF

    更新日期:1984-04-01 00:00:00

  • Sulindac is excreted into bile by a canalicular bile salt pump and undergoes a cholehepatic circulation in rats.

    abstract:BACKGROUND & AIMS:Dihydroxy bile acids induce a bicarbonate-rich hypercholeresis when secreted into canalicular bile in unconjugated form; the mechanism is cholehepatic shunting. The aim of this study was to identify a xenobiotic that induces hypercholeresis by a similar mechanism. METHODS:Five organic acids (sulindac...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/s0016-5085(99)70356-2

    authors: Bolder U,Trang NV,Hagey LR,Schteingart CD,Ton-Nu HT,Cerrè C,Elferink RP,Hofmann AF

    更新日期:1999-10-01 00:00:00

  • Primary biliary cirrhosis is associated with a genetic variant in the 3' flanking region of the CTLA4 gene.

    abstract:BACKGROUND & AIMS:Genetic variation is invoked as a strong component underlying primary biliary cirrhosis (PBC) and other autoimmune disorders. Data suggest that some of this genetic risk is shared, affecting function of the immune mechanisms controlling self-tolerance. Cytotoxic T-lymphocyte antigen 4 (CTLA4) encodes ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2008.06.077

    authors: Juran BD,Atkinson EJ,Schlicht EM,Fridley BL,Lazaridis KN

    更新日期:2008-10-01 00:00:00

  • Mortality in celiac disease.

    abstract::The mortality experienced by a cohort of 653 patients with celiac disease in Edinburgh and the Lothian region has been analyzed. Mortality overall was 1.9-fold (95% confidence limits, 1.5-2.2) that of the general population (115 deaths observed, 61.8 expected; p less than 0.0001). The increased mortality was greatest ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(89)90060-7

    authors: Logan RF,Rifkind EA,Turner ID,Ferguson A

    更新日期:1989-08-01 00:00:00

  • Effects of corticotropin-releasing factor on plasma motilin and somatostatin levels and gastrointestinal motility in dogs.

    abstract::The effects of intracerebroventricular and intravenous administration of corticotropin-releasing factor (CRF) on gastrointestinal motility, motilin-induced gastric motor response, and plasma motilin and somatostatin levels were investigated in fasted dogs chronically prepared with strain gauge transducers on the antru...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(86)90690-6

    authors: Bueno L,Fargeas MJ,Gue M,Peeters TL,Bormans V,Fioramonti J

    更新日期:1986-10-01 00:00:00

  • Morbidity and mortality in compensated cirrhosis type C: a retrospective follow-up study of 384 patients.

    abstract:BACKGROUND & AIMS:Few data are available concerning the long-term prognosis of chronic liver disease associated with hepatitis C virus infection. This study examined the morbidity and survival of patients with compensated cirrhosis type C. METHODS:A cohort of 384 European cirrhotic patients was enrolled at seven terti...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/gast.1997.v112.pm9024300

    authors: Fattovich G,Giustina G,Degos F,Tremolada F,Diodati G,Almasio P,Nevens F,Solinas A,Mura D,Brouwer JT,Thomas H,Njapoum C,Casarin C,Bonetti P,Fuschi P,Basho J,Tocco A,Bhalla A,Galassini R,Noventa F,Schalm SW,Realdi

    更新日期:1997-02-01 00:00:00

  • Anti-rotavirus protein reduces stool output in infants with diarrhea: a randomized placebo-controlled trial.

    abstract:BACKGROUND & AIMS:Rotavirus infection is a leading cause of morbidity and mortality in children younger than 5 years of age. Current treatment options are limited. We assessed the efficacy of a llama-derived, heavy-chain antibody fragment called anti-rotavirus protein (ARP1), in modifying the severity and duration of d...

    journal_title:Gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.1053/j.gastro.2013.06.053

    authors: Sarker SA,Jäkel M,Sultana S,Alam NH,Bardhan PK,Chisti MJ,Salam MA,Theis W,Hammarström L,Frenken LG

    更新日期:2013-10-01 00:00:00

  • Interactions between the dietary polyunsaturated fatty acid ratio and genetic factors determine susceptibility to pediatric Crohn's disease.

    abstract::Increased dietary ratios of ω6/ω3 polyunsaturated fatty acids have been implicated in the pathogenesis of Crohn's disease (CD), but epidemiologic data are limited. We investigated whether variants of genes that control polyunsaturated fatty acid metabolism (CYP4F3, FADS1, and FADS2), along with the dietary ratio of ω6...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2013.12.034

    authors: Costea I,Mack DR,Lemaitre RN,Israel D,Marcil V,Ahmad A,Amre DK

    更新日期:2014-04-01 00:00:00

  • Nitric oxide production in experimental alcoholic liver disease in the rat: role in protection from injury.

    abstract:BACKGROUND & AIMS:Regulation of blood flow and oxygen supply are important pathogenetic factors in alcoholic liver disease. Because nitric oxide may have an important role, its effects on alcoholic liver injury were investigated. METHODS:Rats were fed ethanol intragastrically with either saturated fat or corn oil. Spo...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(95)90400-x

    authors: Nanji AA,Greenberg SS,Tahan SR,Fogt F,Loscalzo J,Sadrzadeh SM,Xie J,Stamler JS

    更新日期:1995-09-01 00:00:00

  • Fibromodulin, an oxidative stress-sensitive proteoglycan, regulates the fibrogenic response to liver injury in mice.

    abstract:BACKGROUND & AIMS:Collagen I deposition contributes to liver fibrosis, yet little is known about other factors that mediate this process. Fibromodulin is a liver proteoglycan that regulates extracellular matrix organization and is induced by fibrogenic stimuli. We propose that fibromodulin contributes to the pathogenes...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2011.11.029

    authors: Mormone E,Lu Y,Ge X,Fiel MI,Nieto N

    更新日期:2012-03-01 00:00:00

  • Effects of somatostatin on gallbladder emptying.

    abstract::Because approximately 80% of reported patients with somatostatinomas have gallstones, this study was performed to determine the effects of exogenous somatostatin on gallbladder emptying responses to liquid and solid meals, direct cholinergic stimulation by bethanechol, indirect cholinergic stimulation by sham feeding,...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(87)90961-9

    authors: Fisher RS,Rock E,Levin G,Malmud L

    更新日期:1987-04-01 00:00:00

  • Proton magnetic resonance spectroscopy studies on human brain myo-inositol in hypo-osmolarity and hepatic encephalopathy.

    abstract:BACKGROUND/AIMS:Recent in vivo studies using proton magnetic resonance (1H-MR) spectroscopy showed low levels of myo-inositol in the brain in hepatic encephalopathy; the pathogenetic relevance of this observation is unclear. METHODS:Myo-inositol and glutamine levels in the brain were studied in vivo by 1H-MR spectrosc...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(94)90552-5

    authors: Häussinger D,Laubenberger J,vom Dahl S,Ernst T,Bayer S,Langer M,Gerok W,Hennig J

    更新日期:1994-11-01 00:00:00

  • Low rate of large polyps (>9 mm) within 10 years after an adequate baseline colonoscopy with no polyps.

    abstract:BACKGROUND & AIMS:Guidelines recommend a 10-year interval between screening colonoscopies with negative results for average-risk individuals. However, many patients are examined at shorter intervals. We investigated outcomes of individuals with no polyps who had repeat colonoscopy in <10 years. METHODS:Data were colle...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2014.04.020

    authors: Lieberman DA,Holub JL,Morris CD,Logan J,Williams JL,Carney P

    更新日期:2014-08-01 00:00:00

  • Verapamil reversal of serotonin-induced jejunal secretion of water and electrolytes in awake dogs.

    abstract::Intestinal handling of water and electrolytes was monitored in 5 conscious dogs with chronic 25-cm Thiry-Vella loops of proximal jejunum using a neutral isosmotic perfusate containing [14C]polyethylene glycol as a recovery marker. Under basal conditions the animals absorbed water, Na+, and Cl-, and there was minimal n...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(86)91102-9

    authors: Zinner MJ,McFadden D,Sherlock D,Jaffe BM

    更新日期:1986-03-01 00:00:00

  • Major histocompatibility antigen-restricted cytotoxicity in inflammatory bowel disease.

    abstract:BACKGROUND:The role of cytotoxicity mediated by peripheral blood mononuclear cells for colonic epithelial cells in inflammatory bowel disease (IBD) is still controversial. To clarify it, we studied major histocompatibility antigen (MHC)-restricted T cell-mediated cytotoxicity (CTL). METHODS:Cytotoxicity was measured b...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(93)90405-2

    authors: Okazaki K,Morita M,Nishimori I,Sano S,Toyonaga M,Nakazawa Y,Yamamoto Y,Yamamoto Y

    更新日期:1993-02-01 00:00:00

  • Direct ex vivo analysis of hepatitis B virus-specific CD8(+) T cells associated with the control of infection.

    abstract:BACKGROUND & AIMS:Cytotoxic T cells have been suggested to be responsible for lysis of hepatitis B virus (HBV)-infected hepatocytes and control of virus infection. The frequency, kinetics, phenotype, and capacity for clonal expansion of circulating HBV-specific CD8 cells were analyzed directly in patients with acute HB...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/s0016-5085(99)70289-1

    authors: Maini MK,Boni C,Ogg GS,King AS,Reignat S,Lee CK,Larrubia JR,Webster GJ,McMichael AJ,Ferrari C,Williams R,Vergani D,Bertoletti A

    更新日期:1999-12-01 00:00:00

  • Small intestinal epithelial renewal in the Syrian hamster exposed to cholera enterotoxin.

    abstract::Epithelial renewal of the small intestine was measured in the Syrian hamster utilizing tritiated thymidine by standard autoradiographic and a scintillation counting technique. Scintillation counting of intestinal replicates proved to be as accurate as standard autoradiography. Average mucosal cell turnover was 71 +/- ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Banwell JG,Lepot A,Hanke DW,Sigdestad C

    更新日期:1978-10-01 00:00:00

  • Serine proteases excite myenteric neurons through protease-activated receptors in guinea pig small intestine.

    abstract:BACKGROUND & AIMS:Serine proteases are postulated to influence gastrointestinal function by stimulating protease-activated receptors (PARs). This study identified the effects on myenteric neurons of activating PARs and investigated underlying mechanisms of action. METHODS:Intracellular electrophysiologic methods were ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/gast.2002.36581

    authors: Gao C,Liu S,Hu HZ,Gao N,Kim GY,Xia Y,Wood JD

    更新日期:2002-11-01 00:00:00

  • Intestinal permeability: an overview.

    abstract::The noninvasive assessment of intestinal permeability in humans has a 20-year history. Because the tests are increasingly used in clinical practice and research and because there is much controversy, we reviewed the literature and outlined the potential and possible shortcomings of these procedures. Data was obtained ...

    journal_title:Gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1016/0016-5085(95)90708-4

    authors: Bjarnason I,MacPherson A,Hollander D

    更新日期:1995-05-01 00:00:00

  • Helicobacter pylori in the drinking water in Peru.

    abstract:BACKGROUND & AIMS:An association between water sources and the prevalence of Helicobacter pylori infection in Peruvian children was shown previously. The aim of this study was to confirm the presence of H. pylori in drinking water in the same community. METHODS:Forty-eight drinking water samples from different locatio...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/gast.1996.v110.pm8612990

    authors: Hulten K,Han SW,Enroth H,Klein PD,Opekun AR,Gilman RH,Evans DG,Engstrand L,Graham DY,El-Zaatari FA

    更新日期:1996-04-01 00:00:00

  • Role of calcitonin gene-related peptide and nitric oxide in the gastroprotective effect of capsaicin in the rat.

    abstract:BACKGROUND:Capsaicin-sensitive neurons contain various peptides including calcitonin gene-related peptide. This study examines (1) whether calcitonin gene-related peptide is involved in capsaicin-induced gastroprotection and (2) whether nitric oxide and prostaglandin are required for calcitonin gene-related peptide to ...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(93)90345-d

    authors: Lambrecht N,Burchert M,Respondek M,Müller KM,Peskar BM

    更新日期:1993-05-01 00:00:00

  • Glycemic load, glycemic index, and carbohydrate intake in relation to risk of cholecystectomy in women.

    abstract:BACKGROUND & AIMS:High-carbohydrate diets with a high glycemic response may exacerbate the metabolic consequences of the insulin-resistance syndrome. The effect on the incidence of gallstone disease is not clear. METHODS:We examined the associations between high-carbohydrate diets with a high glycemic response and the...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2005.05.016

    authors: Tsai CJ,Leitzmann MF,Willett WC,Giovannucci EL

    更新日期:2005-07-01 00:00:00

  • A Panel of Methylated MicroRNA Biomarkers for Identifying High-Risk Patients With Ulcerative Colitis-Associated Colorectal Cancer.

    abstract:BACKGROUND & AIMS:Methylation of specific microRNAs (miRNAs) often occurs in an age-dependent manner, as a field defect in some instances, and may be an early event in colitis-associated carcinogenesis. We aimed to determine whether specific mRNA signature patterns (MIR1, MIR9, MIR124, MIR137, MIR34B/C) could be used t...

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1053/j.gastro.2017.08.037

    authors: Toiyama Y,Okugawa Y,Tanaka K,Araki T,Uchida K,Hishida A,Uchino M,Ikeuchi H,Hirota S,Kusunoki M,Boland CR,Goel A

    更新日期:2017-12-01 00:00:00

  • Persistent lack of somatostatin receptors in gastric mucosa of healing ulcers in rats.

    abstract:BACKGROUND/AIMS:Growth factors and their receptors play a role in healing ulcers of the rat. Somatostatin is a putative growth factor in the gastric mucosa with multiple functions mediated by specific receptors. Therefore, the distribution of somatostatin receptors was evaluated in the gastric mucosa of healing ulcers....

    journal_title:Gastroenterology

    pub_type: 杂志文章

    doi:10.1016/0016-5085(94)90157-0

    authors: Reubi JC,Waser B,Schmassmann A,Halter F

    更新日期:1994-08-01 00:00:00