Helicobacter pylori in the drinking water in Peru.


BACKGROUND & AIMS:An association between water sources and the prevalence of Helicobacter pylori infection in Peruvian children was shown previously. The aim of this study was to confirm the presence of H. pylori in drinking water in the same community. METHODS:Forty-eight drinking water samples from different locations in pueblo jovenes (new towns) near Lima were collected. Samples were frozen until technology advanced to the point to the point at which H. pylori might be reliably detected. Immunomagnetic beads coated with anti-H. pylori immunoglobulin Gs were used to concentrate H. pylori, and two polymerase chain reaction assays based on different H. pylori genes were used. One was a polymerase chain reaction for the detection of the H. pylori adhesin subunit encoding gene, and the second was a previously validated H. pylori 16S ribosomal RNA reverse transcriptase-polymerase chain reaction. RESULTS:The expected 375-base pair fragment from the adhesin gene was amplified from 24 water samples. The expected 500-base pair fragment of the 16S ribosomal RNA and the 375-base pair fragment of the adhesin gene were amplified from 11 of the samples. CONCLUSIONS:These results confirm the presence of H. pylori in drinking water in Peru and are consistent with conclusions from a previous epidemiological study of the same population. This provides additional evidence for waterborne transmission of H. pylori in some environments.






Hulten K,Han SW,Enroth H,Klein PD,Opekun AR,Gilman RH,Evans DG,Engstrand L,Graham DY,El-Zaatari FA




Has Abstract


1996-04-01 00:00:00














  • Increased frequency of transient lower esophageal sphincter relaxation induced by gastric distention in reflux patients with hiatal hernia.

    abstract:BACKGROUND & AIMS:This study aimed to determine if hiatal hernia influences vulnerability to reflux and transient lower esophageal sphincter relaxation (tLESR) during gastric distention in patients with gastroesophageal reflux disease (GERD). METHODS:Eight normal subjects and 15 patients with GERD were studied. A meta...


    pub_type: 杂志文章


    authors: Kahrilas PJ,Shi G,Manka M,Joehl RJ

    更新日期:2000-04-01 00:00:00

  • Consumption of n-3 fatty acids and fish reduces risk of hepatocellular carcinoma.

    abstract:BACKGROUND & AIMS:Fish is a rich source of n-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA). Although consumption of fish and n-3 PUFA has been reported to protect against the development of some types of cancer, little is known ab...


    pub_type: 杂志文章


    authors: Sawada N,Inoue M,Iwasaki M,Sasazuki S,Shimazu T,Yamaji T,Takachi R,Tanaka Y,Mizokami M,Tsugane S,Japan Public Health Center-Based Prospective Study Group.

    更新日期:2012-06-01 00:00:00

  • Interleukin 2 activity of human intestinal mucosa mononuclear cells. Decreased levels in inflammatory bowel disease.

    abstract::Interleukin 2, a soluble product of activated T lymphocytes, is of central importance in the development of an appropriate T-cell immune response. Defects in the production of or response to this lymphokine have been described in a variety of immune deficiency and autoimmune states, thus suggesting a role in the patho...


    pub_type: 杂志文章


    authors: Fiocchi C,Hilfiker ML,Youngman KR,Doerder NC,Finke JH

    更新日期:1984-04-01 00:00:00

  • Role of transglutaminase 2 in liver injury via cross-linking and silencing of transcription factor Sp1.

    abstract:BACKGROUND & AIMS:Despite high morbidity and mortality of alcoholic liver disease worldwide, the molecular mechanisms underlying alcohol-induced liver cell death are not fully understood. Transglutaminase 2 (TG2) is a cross-linking enzyme implicated in apoptosis. TG2 levels and activity are increased in association wit...


    pub_type: 杂志文章


    authors: Tatsukawa H,Fukaya Y,Frampton G,Martinez-Fuentes A,Suzuki K,Kuo TF,Nagatsuma K,Shimokado K,Okuno M,Wu J,Iismaa S,Matsuura T,Tsukamoto H,Zern MA,Graham RM,Kojima S

    更新日期:2009-05-01 00:00:00

  • Long-term survival following endoscopic and surgical treatment of high-grade dysplasia in Barrett's esophagus.

    abstract:BACKGROUND & AIMS:Photodynamic therapy (PDT) for high-grade dysplasia (HGD) in Barrett's esophagus is a Food and Drug Administration-approved alternative to esophagectomy. Critical information regarding overall survival of patients followed up long-term after these therapies is lacking. Our aim was to compare the long-...


    pub_type: 杂志文章


    authors: Prasad GA,Wang KK,Buttar NS,Wongkeesong LM,Krishnadath KK,Nichols FC 3rd,Lutzke LS,Borkenhagen LS

    更新日期:2007-04-01 00:00:00

  • Maintenance therapy with peginterferon alfa-2b does not prevent hepatocellular carcinoma in cirrhotic patients with chronic hepatitis C.

    abstract:BACKGROUND & AIMS:Several studies have reported that low doses of interferon can delay the development of hepatocellular carcinoma (HCC) and progression of chronic hepatitis C. We investigated the incidence of clinical events among participants of the Evaluation of PegIntron in Control of Hepatitis C Cirrhosis (EPIC)3 ...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Bruix J,Poynard T,Colombo M,Schiff E,Burak K,Heathcote EJ,Berg T,Poo JL,Mello CB,Guenther R,Niederau C,Terg R,Bedossa P,Boparai N,Griffel LH,Burroughs M,Brass CA,Albrecht JK,EPIC3 Study Group.

    更新日期:2011-06-01 00:00:00

  • Temozolomide increases the number of mismatch repair-deficient intestinal crypts and accelerates tumorigenesis in a mouse model of Lynch syndrome.

    abstract:BACKGROUND & AIMS:Lynch syndrome, a nonpolyposis form of hereditary colorectal cancer, is caused by inherited defects in DNA mismatch repair (MMR) genes. Most patients carry a germline mutation in 1 allele of the MMR genes MSH2 or MLH1. With spontaneous loss of the wild-type allele, cells with defects in MMR exist amon...


    pub_type: 杂志文章


    authors: Wojciechowicz K,Cantelli E,Van Gerwen B,Plug M,Van Der Wal A,Delzenne-Goette E,Song JY,De Vries S,Dekker M,Te Riele H

    更新日期:2014-11-01 00:00:00

  • Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli.

    abstract:BACKGROUND & AIMS:Intestinal microbes induce homeostatic mucosal immune responses, but can also cause inappropriate immune activation in genetically susceptible hosts. Although immune responses to bacterial products have been studied extensively, little is known about how intestinal inflammation affects functions of co...


    pub_type: 杂志文章


    authors: Patwa LG,Fan TJ,Tchaptchet S,Liu Y,Lussier YA,Sartor RB,Hansen JJ

    更新日期:2011-11-01 00:00:00

  • Failure of colchicine to improve short-term survival in patients with alcoholic hepatitis.

    abstract::Colchicine treatment was used in this randomized placebo-controlled trial in patients with severe acute alcoholic hepatitis [serum bilirubin greater than or equal to 5 mg/dL (85.5 mumol/L) mean, 17.5 +/- 7.5 mg/dL (299.25 +/- 128.25 mumol/L)]. Hospitalization mortality and morbidity and the effect on biochemical test ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Akriviadis EA,Steindel H,Pinto PC,Fong TL,Kanel G,Reynolds TB,Gupta S

    更新日期:1990-09-01 00:00:00

  • Influence of pH on metabolism and urease activity of Helicobacter pylori.

    abstract:BACKGROUND & AIMS:The metabolic and urease responses of Helicobacter pylori to variations in gastric acidity are unknown. The aim of this study was to determine effects of changes of environmental pH on metabolism, urease activity, and survival of H. pylori in an unbuffered environment. METHODS:Bacterial metabolism an...


    pub_type: 杂志文章


    authors: Rektorschek M,Weeks D,Sachs G,Melchers K

    更新日期:1998-09-01 00:00:00

  • Measurement of gastric emptying time by real-time ultrasonography.

    abstract::This paper describes an ultrasound method of assessing gastric emptying time based on measurements of the gastric antrum, which is visible in almost all subjects before and after meals. A total of 54 subjects were examined including 18 normal subjects and 36 subjects with idiopathic functional dyspepsia. The emptying ...


    pub_type: 杂志文章


    authors: Bolondi L,Bortolotti M,Santi V,Calletti T,Gaiani S,Labò G

    更新日期:1985-10-01 00:00:00

  • Prognosis following spontaneous HBsAg seroclearance in chronic hepatitis B patients with or without concurrent infection.

    abstract:BACKGROUND & AIMS:Spontaneous hepatitis B surface antigen (HBsAg) seroclearance is a rare event in patients with chronic hepatitis B virus infection. The aim of this study was to clarify the controversy on long-term prognosis following spontaneous HBsAg seroclearance using a large series of patients. METHODS:A total o...


    pub_type: 杂志文章


    authors: Chen YC,Sheen IS,Chu CM,Liaw YF

    更新日期:2002-10-01 00:00:00

  • Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.

    abstract:BACKGROUND & AIMS:Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-beta signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-beta signaling is impaired because of high expression...


    pub_type: 杂志文章


    authors: Fantini MC,Rizzo A,Fina D,Caruso R,Sarra M,Stolfi C,Becker C,Macdonald TT,Pallone F,Neurath MF,Monteleone G

    更新日期:2009-04-01 00:00:00

  • Increased de novo lipogenesis is a distinct characteristic of individuals with nonalcoholic fatty liver disease.

    abstract:BACKGROUND & AIMS:There have been few studies of the role of de novo lipogenesis in the development of nonalcoholic fatty liver disease (NAFLD). We used isotope analyses to compare de novo lipogenesis and fatty acid flux between subjects with NAFLD and those without, matched for metabolic factors (controls). METHODS:W...


    pub_type: 杂志文章


    authors: Lambert JE,Ramos-Roman MA,Browning JD,Parks EJ

    更新日期:2014-03-01 00:00:00

  • Activated wnt signaling in stroma contributes to development of pancreatic mucinous cystic neoplasms.

    abstract:BACKGROUND & AIMS:Pancreatic mucinous cystic neoplasm (MCN), a cystic tumor of the pancreas that develops most frequently in women, is a potential precursor to pancreatic ductal adenocarcinoma. MCNs develop primarily in the body and tail of the pancreas and are characterized by the presence of a mucinous epithelium and...


    pub_type: 杂志文章


    authors: Sano M,Driscoll DR,De Jesus-Monge WE,Klimstra DS,Lewis BC

    更新日期:2014-01-01 00:00:00

  • Radioimmunoassay of bile acids: development, validation, and preliminary application of an assay for conjugates of chenodeoxycholic acid.

    abstract::A rapid, sensitive, precise radioimmunoassay for conjugates of chenodeoxycholic (chenic) acid has been developed and validated. Immunogen was prepared with chenylglycine couples to bovine serum albumin, emulsified in Freund's complete adjuvant, and injected intracutaneously in rabbits. Antibodies of moderate titer (us...


    pub_type: 杂志文章


    authors: Schalm SW,van Berge-Henegouwen GP,Hofmann AF,Cowen AE,Turcotte J

    更新日期:1977-08-01 00:00:00

  • Acetazolamide inhibits basal and stimulated HCO3- secretion in the human proximal duodenum.

    abstract:BACKGROUND/AIMS:Carbonic anhydrase activity plays a role in electrolyte transport in many tissues. This study examined the effect of the carbonic anhydrase inhibitor acetazolamide on human basal and prostaglandin E2- and acid-stimulated duodenal mucosal bicarbonate secretion and transmucosal electrical potential differ...


    pub_type: 临床试验,杂志文章


    authors: Knutson TW,Koss MA,Hogan DL,Isenberg JI,Knutson L

    更新日期:1995-01-01 00:00:00

  • Impaired uptake of serotonin by platelets from patients with irritable bowel syndrome correlates with duodenal immune activation.

    abstract:BACKGROUND & AIMS:Patients with irritable bowel syndrome with diarrhea (IBS-D) have increased mucosal serotonin (5-hydroxytryptamine [5-HT]) availability, possibly because immune activation reduces activity of the 5-HT transporter (SERT). We investigated the relationship between mucosal and platelet SERT and immune act...


    pub_type: 杂志文章


    authors: Foley S,Garsed K,Singh G,Duroudier NP,Swan C,Hall IP,Zaitoun A,Bennett A,Marsden C,Holmes G,Walls A,Spiller RC

    更新日期:2011-05-01 00:00:00

  • Intestinal clearance of alpha 1-antitrypsin. A sensitive method for the detection of protein-losing enteropathy.

    abstract::Gastrointestinal loss of plasma is usually measured with radiolabeled macromolecules. These methods are expensive and cumbersome. The use of alpha 1-antitrypsin as an endogenous marker and the determination of alpha 1-antitrypsin fecal clearance enable the diagnosis of protein-losing enteropathy. alpha 1-Antitrypsin i...


    pub_type: 杂志文章


    authors: Florent C,L'Hirondel C,Desmazures C,Aymes C,Bernier JJ

    更新日期:1981-10-01 00:00:00

  • Ultrasonic properties of gallstones. Effect of stone size and composition.

    abstract::In vivo and in vitro ultrasonic characteristics of gallstones were compared with stone size and composition with the aim of understanding the basis for these features. In vivo and in vitro ultrasonic features of the same stone were identical. Acoustic shadowing was not due to stone type, radiodensity, or calcium conte...


    pub_type: 杂志文章


    authors: Good LI,Edell SL,Soloway RD,Trotman BW,Mulhern C,Arger PA

    更新日期:1979-08-01 00:00:00

  • Monozygotic triplets with Crohn's disease of the colon.

    abstract::Female monozygotic triplets all developed Crohn's disease within a period of 11 mo. The monozygosity was demonstrated by analysis of 24 genetic markers of different chromosomal localization. Crohn's disease was diagnosed using clinical, radiologic, and endoscopic findings. Histologic evaluation of bowel tissue was con...


    pub_type: 杂志文章


    authors: Purrmann J,Bertrams J,Borchard F,Miller B,Cleveland S,Stolze T,Strohmeyer G

    更新日期:1986-12-01 00:00:00

  • Fundic inhibition of acid secretion and gastrin release in the dog.

    abstract::In dogs provided with Heidenhain pouch and gastric fistula, we studied the effect of near-total excision of the fundic mucosa of the main stomach on acid secretion and gastrin release. Fundic mucosal excision abolished the mean response of the gastric fistula and reduced its maximal response to pentagastrin and to his...


    pub_type: 杂志文章


    authors: Soon-Shiong P,Debas HT

    更新日期:1980-11-01 00:00:00

  • Hepatocellular Carcinomas With Mutational Activation of Beta-Catenin Require Choline and Can Be Detected by Positron Emission Tomography.

    abstract:BACKGROUND & AIMS:In one-third of hepatocellular carcinomas (HCCs), cancer cells have mutations that activate β-catenin pathway. These cells have alterations in glutamine, bile, and lipid metabolism. We investigated whether positron emission tomography (PET) imaging allows identification of altered metabolic pathways t...


    pub_type: 杂志文章


    authors: Gougelet A,Sartor C,Senni N,Calderaro J,Fartoux L,Lequoy M,Wendum D,Talbot JN,Prignon A,Chalaye J,Imbeaud S,Zucman-Rossi J,Tordjmann T,Godard C,Bossard P,Rosmorduc O,Amaddeo G,Colnot S

    更新日期:2019-09-01 00:00:00

  • Use of selective mesenteric vasodilator peptides in experimental nonocclusive mesenteric ischemia in the dog.

    abstract::Three structurally related peptides, ovine corticotropin-releasing factor, sauvagine, and urotensin I are selective mesenteric vasodilators in dogs. To assess the possible benefit of these peptides in nonocclusive mesenteric ischemia, they were compared with a nonselective vasodilator, sodium nitroprusside, in the ane...


    pub_type: 杂志文章


    authors: MacCannell KL,Newton CA,Lederis K,Rivier J,Tiffany M

    更新日期:1986-03-01 00:00:00

  • Accumulation and cellular localization of fibrinogen/fibrin during short-term and long-term rat liver injury.

    abstract:BACKGROUND/AIMS:During liver fibrosis, there is a putative pacemaker role of fibronectin. Fibrinogen is closely linked to fibronectin during clotting processes. The aim of this study was to show fibrinogen gene expression during liver damage. METHODS:Fibrinogen/fibrin deposition in damaged livers was studied by immuno...


    pub_type: 杂志文章


    authors: Neubauer K,Knittel T,Armbrust T,Ramadori G

    更新日期:1995-04-01 00:00:00

  • Adenine arabinoside therapy in HBsAg-positive chronic liver disease: a controlled study.

    abstract::A controlled trial has been undertaken to evaluate adenine arabinoside in the treatment of hepatitis B surface antigen-positive chronic liver disease. Thirteen patients (7 hepatitis B virus DNA polymerase and hepatitis B e antigen-positive, 6 DNA polymerase negative and hepatitis B e antibody-positive) were treated wi...


    pub_type: 临床试验,杂志文章


    authors: Bassendine MF,Chadwick RG,Salmeron J,Shipton U,Thomas HC,Sherlock S

    更新日期:1981-05-01 00:00:00

  • Proton magnetic resonance spectroscopy studies on human brain myo-inositol in hypo-osmolarity and hepatic encephalopathy.

    abstract:BACKGROUND/AIMS:Recent in vivo studies using proton magnetic resonance (1H-MR) spectroscopy showed low levels of myo-inositol in the brain in hepatic encephalopathy; the pathogenetic relevance of this observation is unclear. METHODS:Myo-inositol and glutamine levels in the brain were studied in vivo by 1H-MR spectrosc...


    pub_type: 杂志文章


    authors: Häussinger D,Laubenberger J,vom Dahl S,Ernst T,Bayer S,Langer M,Gerok W,Hennig J

    更新日期:1994-11-01 00:00:00

  • Inhibition of water and electrolyte absorption by polyethylene glycol (PEG).

    abstract::Polyethylene glycol (PEG) is commonly used as a nonabsorbable volume marker in intestinal perfusion and flow studies. It has been assumed that PEG does not affect water and electrolyte movement, but this has not been extensively investigated. Using triple-lumen tube perfusion technique, we examined the effect of vario...


    pub_type: 杂志文章


    authors: Davis GR,Santa Ana CA,Morawski SG,Fordtran JS

    更新日期:1980-07-01 00:00:00

  • Combined targeted treatment to eliminate tumorigenic cancer stem cells in human pancreatic cancer.

    abstract:BACKGROUND & AIMS:Pancreatic cancers contain exclusively tumorigenic cancer stem cells (CSCs), which are highly resistant to chemotherapy, resulting in a relative increase in CSC numbers during gemcitabine treatment. Signaling through sonic hedgehog and mammalian target of rapamycin (mTOR), respectively, may be essenti...


    pub_type: 杂志文章


    authors: Mueller MT,Hermann PC,Witthauer J,Rubio-Viqueira B,Leicht SF,Huber S,Ellwart JW,Mustafa M,Bartenstein P,D'Haese JG,Schoenberg MH,Berger F,Jauch KW,Hidalgo M,Heeschen C

    更新日期:2009-09-01 00:00:00

  • Preparation of subcellular membranes from rat intestinal scrapings or isolated cells. Different Ca2+ binding, nonesterified fatty acid levels, and lipolytic activity.

    abstract::Basolateral, brush-border, and Golgi-enriched subcellular membrane fractions, prepared from homogenates of rat small intestinal mucosa obtained by scraping, had unusually high concentrations of nonesterified fatty acids. These fatty acids appear to be responsible for the large amount of calcium binding, an effect that...


    pub_type: 杂志文章


    authors: Walters JR,Horvath PJ,Weiser MM

    更新日期:1986-07-01 00:00:00