Abstract:
:Peritoneal invasion through the mesothelial cell layer is a hallmark of ovarian cancer metastasis. Using tissue engineering technologies, we recreated an ovarian tumor microenvironment replicating this aspect of disease progression. Ovarian cancer cell-laden hydrogels were combined with mesothelial cell-layered melt electrospun written scaffolds and characterized with proliferation and transcriptomic analyses and used as intraperitoneal xenografts. Here we show increased cancer cell proliferation in these 3D co-cultures, which we validated using patient-derived cells and linked to peritoneal tumor growth in vivo. Transcriptome-wide expression analysis identified IGFBP7, PTGS2, VEGFC and FGF2 as bidirectional factors deregulated in 3D co-cultures compared to 3D mono-cultures, which we confirmed by immunohistochemistry of xenograft and patient-derived tumor tissues and correlated with overall and progression-free survival. These factors were further increased upon expression of kallikrein-related proteases. This clinically predictive model allows us to mimic the complexity and processes of the metastatic disease that may lead to therapies that protect from peritoneal invasion or delay the development of metastasis.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Loessner D,Rockstroh A,Shokoohmand A,Holzapfel BM,Wagner F,Baldwin J,Boxberg M,Schmalfeldt B,Lengyel E,Clements JA,Hutmacher DWdoi
10.1016/j.biomaterials.2018.10.014subject
Has Abstractpub_date
2019-01-01 00:00:00pages
63-75eissn
0142-9612issn
1878-5905pii
S0142-9612(18)30722-1journal_volume
190-191pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::This study was designed to examine the adhesion, proliferation, and morphology of chondrocytes on new scaffolds; and to examine these cells histologically for the ability of the chondrocytes to maintain chondrogenic properties after subcutaneous implantation into nude mice. Both 75:25 poly (L-lactide-co-epsilon-caprol...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00210-2
更新日期:2003-09-01 00:00:00
abstract::Patient-derived tumor organoids offer potentially useful models of cancer tissue physiology. Yet, conventional organoid cultures utilize generic matrices that are difficult to tailor for various unique tumor microenvironments. Here, we employ synthetic, enzymatically crosslinked hydrogels to define mechanical and bioc...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119400
更新日期:2019-10-01 00:00:00
abstract::Cochlear implant (CI) is a successful device to restore hearing. Despite continuous development, frequency discrimination is poor in CI users due to an anatomical gap between the auditory neurons and CI electrode causing current spread and unspecific neural stimulation. One strategy to close this anatomical gap is gui...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.12.020
更新日期:2017-04-01 00:00:00
abstract::To maintain enzymatic activity during long-term storage by conjugation with water-soluble 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers (PMPC-COOH) having various molecular weights with a carboxylic group on the terminal, such compounds were synthesized as a polymer modifier using a photoinduced living radic...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00474-5
更新日期:2004-01-01 00:00:00
abstract::Bioceramic coatings, created by the high velocity oxy-fuel combustion spraying of hydroxyapatite (HA) powders onto commercially pure titanium, were characterized in order to determine whether this relatively new coating process can be successfully applied to bioceramic coatings of orthopaedic and dental implants. Four...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(95)92122-m
更新日期:1995-02-01 00:00:00
abstract::Natural materials exhibit anisotropy with variations in soluble factors, cell distribution, and matrix properties. The ability to recreate the heterogeneity of the natural materials is a major challenge for investigating cell-material interactions and for developing biomimetic materials. Here we present a generic flui...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.12.012
更新日期:2010-03-01 00:00:00
abstract::Poly(L-lactide) (PLLA) with an extremely high molecular weight (Mv up to 1 X 10(6)) was synthesized at a low catalyst concentration (0.015 wt%) and temperatures between 100-110 degrees C. Besides good mechanical properties the as-polymerized PLLA exhibited a microporous structure. Plates and screws of this material we...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(87)90034-2
更新日期:1987-01-01 00:00:00
abstract::A tissue-engineered substitute that facilitates large-volume regeneration of the subcutaneous adipose tissue layer is needed for reconstructive plastic surgery. Towards this goal, we describe the in vitro culture of primary human adipose-derived stem cells (ASC) seeded into placental decellular matrix (PDM) and cross-...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.12.028
更新日期:2008-04-01 00:00:00
abstract::Highly crosslinked UHMWPEs have demonstrated improved in vitro wear properties; however, there is concern regarding loss of fracture resistance and ductility. The goals of this study were to evaluate the micromechanisms of failure under uniaxial tension and to determine the effect of gamma radiation-induced crosslinki...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00277-1
更新日期:2003-10-01 00:00:00
abstract::The interactions between alginate and polycations have been studied by using different labelling techniques. Binding of poly-L-lysine (PLL) to alginate in the gel state is mainly governed by the amount of dissociable negative charges on the bead surface. PLL was found to bind more rapidly to gel beads made from algina...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(96)84680-1
更新日期:1996-05-01 00:00:00
abstract::Four commercial hydroxyapatites (both natural and synthetic) were tested to assess transformations of the chemical and crystalline structure following variation of temperature from 20 to 1600 degrees C. The thermal behaviour of hydroxyapatite is relevant for biomedical applications such as plasma spraying of metallic ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(93)90146-s
更新日期:1993-05-01 00:00:00
abstract::In an effort to examine liver injury, immune response, and other physiological effects in mice caused by intragastric administration of nanoparticulate anatase titanium dioxide (5nm), we assessed T lymphocytes, B lymphocyte and NK lymphocyte counts, hematological indices, biochemical parameters of liver functions, and...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.10.003
更新日期:2010-02-01 00:00:00
abstract::Exosomes have emerged as appealing candidate therapeutic agents and delivery nanoplatforms due to their endogenous features and unique biological properties. However, obstacles such as low isolation yield, considerable complexity and potential safety concerns, and inefficient drug payload substantially hamper their th...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2020.119925
更新日期:2020-03-02 00:00:00
abstract::Semisynthetic network alginate polymer (SNAP), synthesized by acetalization of linear alginate with di-aldehyde, is a pH-responsive tetrafunctionally linked 3D gel network, and has potential application in oral delivery of protein therapeutics and active biologicals, and as tissue bioscaffold for regenerative medicine...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.07.034
更新日期:2009-10-01 00:00:00
abstract::Literature has shown that slowing down the curing rate of light-initiated resin composites by lowering the light intensity of the light source improved the integrity of the adhesive composite-cavity interface as a result of a more gradual contraction stress development. This study investigated how the curing rate coul...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(96)00074-9
更新日期:1996-12-01 00:00:00
abstract::Microcomputed tomography (micro-CT) is increasingly being used to analyze the three-dimensional structure and architecture of microvascular networks. Therefore we have evaluated a micro-CT analysis of VEGF-induced vessel ingrowth into a porous polyurethane scaffold through comparison with analyses by CD31 immunohistoc...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.07.044
更新日期:2009-10-01 00:00:00
abstract::Covalent immobilization of cell-adhesive proteins such as collagen and fibronectin on to the surface of poly(vinyl alcohol) hydrogel was investigated by using diisocyanates, polyisocyanates, and cyanogen bromide. It was found that 0.5 and 12 micrograms/cm2 of collagen were immobilized on to the surface by using hexame...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90024-5
更新日期:1991-10-01 00:00:00
abstract::Polycaprolactone (PCL) membrane was modified by grafting copolymerization of methacrylic acid (MAA) initiated under UV light. The covalent immobilization of gelatin on PCL-g-PMAA surface was consequently performed by using condensing agent, 1-ethyl-3-(3-dimethylamino propyl) carbodiimide hydrochloride. The occurrence ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00247-8
更新日期:2002-12-01 00:00:00
abstract::The design of peptide-based subunit vaccine formulations for the direct delivery of tumor antigen peptides (Aps) to dendritic cells (DCs) localized within draining lymph nodes (DLNs) is challenging. Mature DCs (mDCs) are abundantly distributed within DLNs but have dramatically reduced endocytic uptake and antigen-proc...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.05.008
更新日期:2016-08-01 00:00:00
abstract::Over the past two decades, in vitro autologous endothelial cell (EC) coverage of expanded polytetrafluoroethylene (ePTFE) graft has been developed and clinically applied with success in infrainguinal bypasses. Before endothelialization, the luminal surface of the graft has to be coated with a currently used fibrinolyt...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.01.011
更新日期:2005-08-01 00:00:00
abstract::The differentiation of odontoblasts is initiated by the organization of differentiating ameloblasts during tooth formation. However, the exact roles of ameloblast-derived factors in odontoblast differentiation have not yet been characterized. We investigated the effects of preameloblast-conditioned medium (PA-CM) on t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.09.007
更新日期:2011-12-01 00:00:00
abstract::Human embryonic stem cells (hESCs) are generally induced to differentiate by forming spherical structures termed embryoid bodies (EBs) in the presence of soluble growth factors. hEBs are generated by suspending small clumps of hESC colonies; however, the resulting hEBs are heterogeneous because this method lacks the a...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.04.001
更新日期:2014-07-01 00:00:00
abstract::Chondrogenic differentiation of mesenchymal stem cells (MSCs) is critical for successful cartilage regeneration. Several methods have been developed to attempt to chondrogenic differentiation, because chondrogenic differentiated cells can form stable cartilage and induce expression of a cartilage-specific phenotype. I...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.03.025
更新日期:2012-06-01 00:00:00
abstract::This is the first report on a series of studies of the crystallinity of bone tissues. The measurements were done at the microfocused diffraction beamline at ERSF (European Synchrotron Radiation Facility) as a feasibility test on various aspects on microdiffraction analysis. Beside the crystal structure, crystallite si...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(98)00120-3
更新日期:1998-11-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.04.046
更新日期:2008-06-03 00:00:00
abstract::Radiolabeled nanomaterials, especially those with ultra-small structures, have been the research focus in recent years, and thus may open up new prospects for clinical diseases theranostics. Herein, fluorinated Pd nanosheets labeled with Gd or radionuclides are developed as multimodal platforms for tumor theranostics....
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.06.040
更新日期:2018-10-01 00:00:00
abstract::Matrix anisotropy is important for long term in vivo functionality. However, it is not fully understood how to guide matrix anisotropy in vitro. Experiments suggest actin-mediated cell traction contributes. Although F-actin in 2D displays a stretch-avoidance response, 3D data are lacking. We questioned how cyclic stre...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.06.103
更新日期:2012-10-01 00:00:00
abstract::Elemental metal nanoparticles like cadmium and silver are known to cause oxidative stress and are also highly toxic. Yet for gold nanoparticles (AuNPs), it is not well established whether these particles are biologically toxic. Here we show that AuNPs, which were taken up by MRC-5 human lung fibroblasts in vitro, indu...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.04.014
更新日期:2010-08-01 00:00:00
abstract::Embryonic stem cells (ESCs) are promising cell sources for cell-based therapy. It has been established that the formation of ESC aggregates promotes their differentiation into the derivatives of all three germ layers. ESC aggregates are generally prepared via the formation of suspended spherical aggregates called embr...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.05.003
更新日期:2009-09-01 00:00:00
abstract::Although folate exhibits many advantages over other targeting ligands, it has one major defect: poor water solubility. Once it was conjugated to hydrophilic drug carrier such as N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer, the hydrophobic folate may be buried inside the random polymer coil and not exposed to b...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.03.027
更新日期:2014-06-01 00:00:00