Transcriptome and proteome analyses of the molecular mechanisms associated with coix seed nutritional quality in the process of breeding.

Abstract:

:In present study, the content of main nutrients in wild coix seed were significantly higher than cultivated coix seed. Transcriptome and proteome were combined to provide new insight of the molecular mechanisms linked to nutritional quality of wild coix seed and cultivated coix seed by RNA sequencing and isobaric tags for relative and absolute quantification techniques. A total of 20,039 genes and 471 proteins exhibited differential expression level in cultivated coix seed when compared with wild coix seed. These genes and proteins revealed that the pathway of flavonoids biosynthesis, starch and sucrose metabolism, lipid metabolism and amino acid metabolism were linked to nutritional quality of coix seed. Our results provided transcriptomics and proteomics information with respect to the molecular mechanisms of nutritional changes of coix seed, identified key genes and proteins that associated with the metabolism and accumulation of nutrients, and helped clarify the mechanisms of nutrient differences.

journal_name

Food Chem

journal_title

Food chemistry

authors

Huang Q,Xu M,Zhang H,He D,Kong Y,Chen L,Song H

doi

10.1016/j.foodchem.2018.07.116

subject

Has Abstract

pub_date

2019-01-30 00:00:00

pages

549-558

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(18)31262-7

journal_volume

272

pub_type

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