Abstract：
:A highly sensitive TaqMan real-time PCR assay targeting the mitochondrial 12S rRNA gene was developed for detection of an avian-specific DNA fragment (68bp) in farm animal and pet feeds. The specificity of the assay was verified against a wide representation of animal and plant species. Applicability assessment of the avian real-time PCR was conducted through representative analysis of two types of compound feeds: industrial farm animal feeds (n=60) subjected to extreme temperatures, and commercial dog and cat feeds (n=210). Results obtained demonstrated the suitability of the real-time PCR assay to detect the presence of low percentages of highly processed avian material in the feed samples analysed. Although quantification results were well reproducible under the experimental conditions tested, an accurate estimation of the target content in feeds is impossible in practice. Nevertheless, the method may be useful as an alternative tool for traceability purposes within the framework of feed control.
journal_name
Food Chemjournal_title
Food chemistryauthors
Pegels N,González I,García T,Martín Rdoi
10.1016/j.foodchem.2013.07.031subject
Has Abstractpub_date
2014-01-01 00:00:00pages
39-47eissn
0308-8146issn
1873-7072pii
S0308-8146(13)00959-Xjournal_volume
142pub_type
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