Abstract：
:Anglerfish (Lophius spp.) is consumed worldwide and is an important economic resource though its seven species are often fraudulently interchanged due to their different commercial value, especially when sold in the form of fillets or pieces. Molecular analysis is the only possible mean to verify traceability and counteract fraud. We developed two multiplex PCRs, one end-point and one real-time with melting curve post-amplification analysis, which can even be run with the simplest two-channel thermocyclers. The two methods were tested on seventy-five reference samples. Their specificity was checked in twenty more species of those most commonly available on the market and in other species of the Lophiidae family. Both methods, the choice of which depends on the equipment and budget of the lab, provide a rapid and easy-to-read response, improving both the simplicity and cost-effectiveness of existing methods for identifying Lophius species.
journal_name
Food Chemjournal_title
Food chemistryauthors
Castigliego L,Armani A,Tinacci L,Gianfaldoni D,Guidi Adoi
10.1016/j.foodchem.2014.06.014subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1-9eissn
0308-8146issn
1873-7072pii
S0308-8146(14)00900-5journal_volume
166pub_type
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