Abstract：
:In this work, a duplex PCR-Enzyme Linked Oligonucleotide Assay (ELONA) is reported for the sensitive and reliable detection of pork adulteration in beef and chicken products, two of the most widely consumed meat types in the world. The strategy relies on the use of species-specific tailed primers for duplex amplification and simple dilution of the PCR reactions for direct colorimetric detection via hybridization, eliminating the need for any other post-amplification steps. A high sensitivity was achieved, with as low as 71-188 pg of genomic DNA able to be detected using mixtures of control DNA from each species. The strategy was validated using DNA add-mixtures as well as DNA extracted from raw meat mixtures and 0.5-1% w/w pork could be easily detected when mixed with beef or chicken. The proposed approach is simple, sensitive and cost-effective compared to equivalent commercial kits suitable for detecting adulterant pork levels in meat products.
journal_name
Food Chemjournal_title
Food chemistryauthors
Skouridou V,Tomaso H,Rau J,Bashammakh AS,El-Shahawi MS,Alyoubi AO,O'Sullivan CKdoi
10.1016/j.foodchem.2019.02.095subject
Has Abstractpub_date
2019-07-30 00:00:00pages
354-362eissn
0308-8146issn
1873-7072pii
S0308-8146(19)30418-2journal_volume
287pub_type
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