Abstract:
:The emergence and outgrowth of a population of tumour cells resistant to multiple drugs is a major problem in the chemotherapeutic treatment of cancer. We have used highly drug-resistant cell lines developed in vitro to study the molecular basis of multidrug resistance. In these cell lines high levels of resistance are frequently associated with amplification and overexpression of a small group of genes termed mdr or gp170. Direct evaluation of the role of these genes in multidrug resistance has awaited the isolation of a member of this gene family in a biologically active form. Here we report the isolation of DNA clones complementary to the cellular messenger RNA transcripts of mdr genes and show that high-level expression of a full-length complementary DNA clone in an otherwise drug-sensitive cell confers a complete multidrug-resistant phenotype. Our results demonstrate that overexpression of a single member of the mdr group is sufficient to confer drug resistance. Furthermore, because the cDNA was isolated from a drug-sensitive cell, mutations in the primary sequence of mdr are not required to produce a multidrug-resistance phenotype.
journal_name
Naturejournal_title
Natureauthors
Gros P,Ben Neriah YB,Croop JM,Housman DEdoi
10.1038/323728a0subject
Has Abstractpub_date
1986-10-23 00:00:00pages
728-31issue
6090eissn
0028-0836issn
1476-4687journal_volume
323pub_type
杂志文章相关文献
NATURE文献大全abstract::SAMHD1, an analogue of the murine interferon (IFN)-γ-induced gene Mg11 (ref. 1), has recently been identified as a human immunodeficiency virus-1 (HIV-1) restriction factor that blocks early-stage virus replication in dendritic and other myeloid cells and is the target of the lentiviral protein Vpx, which can relieve ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10623
更新日期:2011-11-06 00:00:00
abstract::Height is a highly heritable, classic polygenic trait with approximately 700 common associated variants identified through genome-wide association studies so far. Here, we report 83 height-associated coding variants with lower minor-allele frequencies (in the range of 0.1-4.8%) and effects of up to 2 centimetres per a...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature21039
更新日期:2017-02-09 00:00:00
abstract::Inherited genetic variation has a critical but as yet largely uncharacterized role in human disease. Here we report a public database of common variation in the human genome: more than one million single nucleotide polymorphisms (SNPs) for which accurate and complete genotypes have been obtained in 269 DNA samples fro...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04226
更新日期:2005-10-27 00:00:00
abstract::Because their molecular signatures are often prominent in extracts of sediments, bacteria are thought to be important contributors to petroleum source beds. It has been shown recently, however, that abundances of biomarkers do not always reflect relative contributions to sedimentary organic carbon (Corg). The contrib...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369224a0
更新日期:1994-05-19 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 新闻
doi:10.1038/d41586-018-07356-3
更新日期:2018-11-01 00:00:00
abstract::The determination of molecular masses from barometric sedimentation profiles, a main topic in ultracentrifugal analysis, is thought to be quantitatively correct for non-interacting particles. Whereas this expectation is justified for uncharged colloids or macromolecules at low volume fractions, early ultracentrifugati...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02672
更新日期:2004-06-24 00:00:00
abstract::T lymphocytes, essential for the generation of a normal immune response, require the presence of the lymphokine interleukin-2 (IL-2) in order to proliferate. Cells that respond to IL-2 possess a surface receptor glycoprotein specific for this lymphokine. We have recently purified and chemically characterized the IL-2 ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/312768a0
更新日期:1984-12-20 00:00:00
abstract::In response to adenosine 5'-diphosphate, the P2Y1 receptor (P2Y1R) facilitates platelet aggregation, and thus serves as an important antithrombotic drug target. Here we report the crystal structures of the human P2Y1R in complex with a nucleotide antagonist MRS2500 at 2.7 Å resolution, and with a non-nucleotide antago...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14287
更新日期:2015-04-16 00:00:00
abstract::A list of authors and their affiliations appears at the end of the paper New-particle formation is a major contributor to urban smog1,2, but how it occurs in cities is often puzzling3. If the growth rates of urban particles are similar to those found in cleaner environments (1-10 nanometres per hour), then existing un...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2270-4
更新日期:2020-05-01 00:00:00
abstract::Long-term depletion of the stratospheric ozone layer contributes to an increase in terrestrial solar ultraviolet-B radiation. This has deleterious effects on living organisms, such as DNA damage. When exposed to elevated ultraviolet-B radiation (UV-B; 280-315 nm), plants display a wide variety of physiological and mor...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35017595
更新日期:2000-07-06 00:00:00
abstract::Transmissible spongiform encephalopathies are associated with accumulation of PrP(Sc), a conformer of a cellular protein called PrP(C). PrP(Sc) is thought to replicate by imparting its conformation onto PrP(C) (ref. 1), yet conformational discrimination between PrP(C) and PrP(Sc) has remained elusive. Because depositi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35044100
更新日期:2000-11-23 00:00:00
abstract::Activation of oncogenes by mechanisms other than genetic aberrations such as mutations, translocations, or amplifications is largely undefined. Here we report a novel isoform of the anaplastic lymphoma kinase (ALK) that is expressed in ∼11% of melanomas and sporadically in other human cancer types, but not in normal t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature15258
更新日期:2015-10-15 00:00:00
abstract::Numerous studies have suggested that biodiversity reduces variability in ecosystem productivity through compensatory effects; that is, a species increases in its abundance in response to the reduction of another in a fluctuating environment. But this view has been challenged on several grounds. Because most studies ha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02850
更新日期:2004-09-09 00:00:00
abstract::Oxygen, one of the most abundant elements on Earth, often forms an undesired interstitial impurity or ceramic phase (such as an oxide particle) in metallic materials. Even when it adds strength, oxygen doping renders metals brittle1-3. Here we show that oxygen can take the form of ordered oxygen complexes, a state in ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0685-y
更新日期:2018-11-01 00:00:00
abstract::The 18-base-pair sequence element AGGTCGACCAGTACTCCG (the Sal box) signals termination of mouse ribosomal gene transcription. This sequence is recognized by a sequence-specific DNA-binding protein, TTF I, which mediates the termination of transcription by RNA polymerase I (pol I). Subsequently, the ends of the primary...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/344559a0
更新日期:1990-04-05 00:00:00
abstract::Acetylcholine and dopamine are key neurotransmitters in the extrapyramidal motor system, where they are thought to lie in a 'functional balance' brought about by interactions between the terminals of the dopamine-containing nigrostriatal tract and the cholinergic interneurones of the striatum. The precise nature of th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/323625a0
更新日期:1986-10-16 00:00:00
abstract::De novo-designed proteins1-3 hold great promise as building blocks for synthetic circuits, and can complement the use of engineered variants of natural proteins4-7. One such designer protein-degronLOCKR, which is based on 'latching orthogonal cage-key proteins' (LOCKR) technology8-is a switch that degrades a protein o...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1425-7
更新日期:2019-08-01 00:00:00
abstract::Many proteins are expressed in distinct embryonic and adult forms. However, in most cases we do not know why the embryonic form of proteins is required. This question can be readily addressed for the acetylcholine receptor (AChR) because developmentally specified modifications of this ligand-gated ion channel can be d...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/335066a0
更新日期:1988-09-01 00:00:00
abstract::A new system has been developed for measuring the forces produced by a small number (less than 5-150) of myosin molecules interacting with a single actin filament in vitro. The technique can resolve forces of less than a piconewton and has a time resolution in the submillisecond range. It can thus detect fluctuations ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/352301a0
更新日期:1991-07-25 00:00:00
abstract::The Haldane model on a honeycomb lattice is a paradigmatic example of a Hamiltonian featuring topologically distinct phases of matter. It describes a mechanism through which a quantum Hall effect can appear as an intrinsic property of a band structure, rather than being caused by an external magnetic field. Although p...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13915
更新日期:2014-11-13 00:00:00
abstract::Cloning and sequencing of the complementary DNA for platelet-derived endothelial cell growth factor indicates that it is a novel factor distinct from previously characterized proteins. The factor, a protein with a relative molecular mass of about 45,000, stimulates endothelial cell growth and chemotaxis in vitro and a...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/338557a0
更新日期:1989-04-13 00:00:00
abstract::The murine cardioviruses, such as the Mengo and encephalomyocarditis viruses, and the bovine aphthoviruses, such as foot-and-mouth disease virus, are distinguished among positive-strand RNA viruses by the presence of long homopolymeric poly(C) tracts within their 5' noncoding sequences. Although the specific lengths (...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/343474a0
更新日期:1990-02-01 00:00:00
abstract::The interneuronal messenger nitric oxide (NO) may play a central role in the processing of olfactory information. Several circuit elements in the mammalian olfactory bulb contain NO synthase or its functional equivalent, NADPH diaphorase. The effects of NO on cellular excitability or circuit dynamics in the olfactory ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369061a0
更新日期:1994-05-05 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41586-020-2064-8
更新日期:2020-03-01 00:00:00
abstract::Trophic control over the expression and membrane distribution of voltage-dependent ion channels is one of the principal organizing events underlying the maturation of excitable cells. The myelin sheath is a major structural determinant of regional ion channel topography in central axons, but the exact molecular signal...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/352431a0
更新日期:1991-08-01 00:00:00
abstract::Macrophages and dendritic cells have key roles in viral infections, providing virus reservoirs that frequently resist antiviral therapies and linking innate virus detection to antiviral adaptive immune responses. Human immunodeficiency virus 1 (HIV-1) fails to transduce dendritic cells and has a reduced ability to tra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10195
更新日期:2011-06-29 00:00:00
abstract::Deep chlorophyll maxima (DCMs) are widespread in large parts of the world's oceans. These deep layers of high chlorophyll concentration reflect a compromise of phytoplankton growth exposed to two opposing resource gradients: light supplied from above and nutrients supplied from below. It is often argued that DCMs are ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04245
更新日期:2006-01-19 00:00:00
abstract::Thyroglobulin (TG) is the protein precursor of thyroid hormones, which are essential for growth, development and the control of metabolism in vertebrates1,2. Hormone synthesis from TG occurs in the thyroid gland via the iodination and coupling of pairs of tyrosines, and is completed by TG proteolysis3. Tyrosine proxim...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-1995-4
更新日期:2020-02-01 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 新闻
doi:10.1038/d41586-018-07678-2
更新日期:2018-12-01 00:00:00
abstract::Muscarinic receptors of cardiac pacemaker and atrial cells are linked to a potassium channel (IK.ACh) by a pertussis toxin-sensitive GTP-binding protein. The dissociation of G-proteins leads to the generation of two potential transducing elements, alpha-GTP and beta gamma. IK.ACh is activated by G-protein alpha- and b...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/337557a0
更新日期:1989-02-09 00:00:00