Sub-piconewton force fluctuations of actomyosin in vitro.

Abstract:

:A new system has been developed for measuring the forces produced by a small number (less than 5-150) of myosin molecules interacting with a single actin filament in vitro. The technique can resolve forces of less than a piconewton and has a time resolution in the submillisecond range. It can thus detect fluctuations of force caused by individual molecular interactions. From analysis of these force fluctuations, the coupling between the enzymatic ATPase activity of actomyosin and the resulting mechanical impulses can be elucidated.

journal_name

Nature

journal_title

Nature

authors

Ishijima A,Doi T,Sakurada K,Yanagida T

doi

10.1038/352301a0

subject

Has Abstract

pub_date

1991-07-25 00:00:00

pages

301-6

issue

6333

eissn

0028-0836

issn

1476-4687

journal_volume

352

pub_type

杂志文章

相关文献

NATURE文献大全
  • Observation of Hanbury Brown-Twiss anticorrelations for free electrons.

    abstract::Fluctuations in the counting rate of photons originating from uncorrelated point sources become, within the coherently illuminated area, slightly enhanced compared to a random sequence of classical particles. This phenomenon, known in astronomy as the Hanbury Brown-Twiss effect, is a consequence of quantum interferenc...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature00911

    authors: Kiesel H,Renz A,Hasselbach F

    更新日期:2002-07-25 00:00:00

  • Postsynaptic hyperpolarization during conditioning reversibly blocks induction of long-term potentiation.

    abstract::Activity-induced changes in the efficacy of synaptic transmission between neurones are central to several prominent theories of learning. In both in vivo and in vitro preparations of the hippocampus, a conditioning high-frequency stimulus delivered to afferent fibres results in a long-term potentiation of synaptic tra...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/320529a0

    authors: Malinow R,Miller JP

    更新日期:1986-04-10 00:00:00

  • Genetic engineering: frost damage trial halted.

    abstract::The University of California at Berkeley has announced the postponement of a planned experiment involving the field testing of bacteria genetically engineered to reduce frost damage to crops. The action came after Jeremy Rifkin, who had earlier filed suit against the National Institutes of Health after its Recombinan...

    journal_title:Nature

    pub_type: 新闻

    doi:10.1038/305564a0

    authors: Budiansky S

    更新日期:1983-10-13 00:00:00

  • Structure of a bacterial 30S ribosomal subunit at 5.5 A resolution.

    abstract::The 30S ribosomal subunit binds messenger RNA and the anticodon stem-loop of transfer RNA during protein synthesis. A crystallographic analysis of the structure of the subunit from the bacterium Thermus thermophilus is presented. At a resolution of 5.5 A, the phosphate backbone of the ribosomal RNA is visible, as are ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/23631

    authors: Clemons WM Jr,May JL,Wimberly BT,McCutcheon JP,Capel MS,Ramakrishnan V

    更新日期:1999-08-26 00:00:00

  • A supernova origin for dust in a high-redshift quasar.

    abstract::Interstellar dust plays a crucial role in the evolution of the Universe by assisting the formation of molecules, by triggering the formation of the first low-mass stars, and by absorbing stellar ultraviolet-optical light and subsequently re-emitting it at infrared/millimetre wavelengths. Dust is thought to be produced...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02930

    authors: Maiolino R,Schneider R,Oliva E,Bianchi S,Ferrara A,Mannucci F,Pedani M,Sogorb MR

    更新日期:2004-09-30 00:00:00

  • Structure and function of telomeres.

    abstract::The DNA of telomeres--the terminal DNA-protein complexes of chromosomes--differs notably from other DNA sequences in both structure and function. Recent work has highlighted its remarkable mode of synthesis by the ribonucleoprotein reverse transcriptase, telomerase, as well as its ability to form unusual structures in...

    journal_title:Nature

    pub_type: 杂志文章,评审

    doi:10.1038/350569a0

    authors: Blackburn EH

    更新日期:1991-04-18 00:00:00

  • Deterministic quantum teleportation of photonic quantum bits by a hybrid technique.

    abstract::Quantum teleportation allows for the transfer of arbitrary unknown quantum states from a sender to a spatially distant receiver, provided that the two parties share an entangled state and can communicate classically. It is the essence of many sophisticated protocols for quantum communication and computation. Photons a...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12366

    authors: Takeda S,Mizuta T,Fuwa M,van Loock P,Furusawa A

    更新日期:2013-08-15 00:00:00

  • Structure of the acrosomal bundle.

    abstract::In the unactivated Limulus sperm, a 60- micro m-long bundle of actin filaments crosslinked by the protein scruin is bent and twisted into a coil around the base of the nucleus. At fertilization, the bundle uncoils and fully extends in five seconds to support a finger of membrane known as the acrosomal process. This bi...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02881

    authors: Schmid MF,Sherman MB,Matsudaira P,Chiu W

    更新日期:2004-09-02 00:00:00

  • Paramyxovirus SV5 and multiple sclerosis.

    abstract::Multiple sclerosis is commonly associated with a local humoral immune response within the central nervous system. A hallmark of this intrathecal response is the presence of electrophoretically demonstrable oligoclonal bands of IgG in the cerebrospinal fluid (CSF) of up to 95% of patients. Observations indicating that ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/338769a0

    authors: Vandvik B,Norrby E

    更新日期:1989-04-27 00:00:00

  • A synthetic peptide ligase.

    abstract::The preparation of synthetic molecules showing the remarkable efficiencies characteristic of natural biopolymer catalysts remains a formidable challenge for chemical biology. Although significant advances have been made in the understanding of protein structure and function, the de novo construction of such systems re...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/39556

    authors: Severin K,Lee DH,Kennan AJ,Ghadiri MR

    更新日期:1997-10-16 00:00:00

  • Actin-based motility of vaccinia virus.

    abstract::The role of the cytoskeleton during viral infection is poorly understood. Here we show, using a combination of mutant and drug studies, that the intracellular enveloped form of vaccinia virus is capable of inducing the formation of actin tails that are strikingly similar to those seen in Listeria, Shigella and Rickett...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/378636a0

    authors: Cudmore S,Cossart P,Griffiths G,Way M

    更新日期:1995-12-07 00:00:00

  • Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants.

    abstract::Aluminium adjuvants, typically referred to as 'alum', are the most commonly used adjuvants in human and animal vaccines worldwide, yet the mechanism underlying the stimulation of the immune system by alum remains unknown. Toll-like receptors are critical in sensing infections and are therefore common targets of variou...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature06939

    authors: Eisenbarth SC,Colegio OR,O'Connor W,Sutterwala FS,Flavell RA

    更新日期:2008-06-19 00:00:00

  • Cooperating with the future.

    abstract::Overexploitation of renewable resources today has a high cost on the welfare of future generations. Unlike in other public goods games, however, future generations cannot reciprocate actions made today. What mechanisms can maintain cooperation with the future? To answer this question, we devise a new experimental para...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature13530

    authors: Hauser OP,Rand DG,Peysakhovich A,Nowak MA

    更新日期:2014-07-10 00:00:00

  • Renewing Felsenstein's phylogenetic bootstrap in the era of big data.

    abstract::Felsenstein's application of the bootstrap method to evolutionary trees is one of the most cited scientific papers of all time. The bootstrap method, which is based on resampling and replications, is used extensively to assess the robustness of phylogenetic inferences. However, increasing numbers of sequences are now ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-018-0043-0

    authors: Lemoine F,Domelevo Entfellner JB,Wilkinson E,Correia D,Dávila Felipe M,De Oliveira T,Gascuel O

    更新日期:2018-04-01 00:00:00

  • K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions.

    abstract::Somatic mutations in the small GTPase K-Ras are the most common activating lesions found in human cancer, and are generally associated with poor response to standard therapies. Efforts to target this oncogene directly have faced difficulties owing to its picomolar affinity for GTP/GDP and the absence of known alloster...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12796

    authors: Ostrem JM,Peters U,Sos ML,Wells JA,Shokat KM

    更新日期:2013-11-28 00:00:00

  • New variation on the translocation of proteins during early biogenesis of apolipoprotein B.

    abstract::Apolipoprotein B (apo B) is crucial for the transport of cholesterol in humans. It is a large secretory protein that mediates the uptake of low-density lipoproteins and renders several forms of lipid droplets soluble in the blood. The binding of lipid by apo B also prevents this hydrophobic protein from precipitating ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/346382a0

    authors: Chuck SL,Yao Z,Blackhart BD,McCarthy BJ,Lingappa VR

    更新日期:1990-07-26 00:00:00

  • Biotechnology regulation: rules for freed organisms planned.

    abstract::The Environmental Protection Agency is preparing to regulate the biotechnology industry, claiming authority under the provisions of the Federal Toxic Substances Control Act (TOSCA) and the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA). In the agency's view, the products of recombinant DNA techniques can...

    journal_title:Nature

    pub_type: 新闻

    doi:10.1038/306005a0

    authors: Earl C

    更新日期:1983-11-03 00:00:00

  • Recruitment of Drosophila Polycomb group proteins to chromatin by DSP1.

    abstract::Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We p...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature03386

    authors: Déjardin J,Rappailles A,Cuvier O,Grimaud C,Decoville M,Locker D,Cavalli G

    更新日期:2005-03-24 00:00:00

  • Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains.

    abstract::The three-dimensional structure of the ligand-binding region of human E-selectin has been determined at 2.0 A resolution. The structure reveals limited contact between the two domains and a coordination of Ca2+ not predicted from other C-type lectins. Structure/function analysis indicates a defined region and specific...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/367532a0

    authors: Graves BJ,Crowther RL,Chandran C,Rumberger JM,Li S,Huang KS,Presky DH,Familletti PC,Wolitzky BA,Burns DK

    更新日期:1994-02-10 00:00:00

  • Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites.

    abstract::Recently, a class of protein antigens of high relative molecular mass (Mt) which can induce protective immunity against blood-stage malaria has been identified. In Plasmodium falciparum the protein has a Mr of approximately 195,000 (P195). It is the precursor of three proteins of Mr 83,000 (83K), 42K and 19K which are...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/317270a0

    authors: Holder AA,Lockyer MJ,Odink KG,Sandhu JS,Riveros-Moreno V,Nicholls SC,Hillman Y,Davey LS,Tizard ML,Schwarz RT

    更新日期:1985-09-19 00:00:00

  • RNA-catalysed RNA polymerization using nucleoside triphosphates.

    abstract::The hypothesis that certain RNA molecules may be able to catalyse RNA replication is central to current theories of the early evolution of life. In support of this idea, we describe here an RNA that synthesizes RNA using the same reaction as that employed by protein enzymes that catalyse RNA polymerization. In the pre...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/382373a0

    authors: Ekland EH,Bartel DP

    更新日期:1996-07-25 00:00:00

  • Different forms of p53 detected by monoclonal antibodies in non-dividing and dividing lymphocytes.

    abstract::The commitment of non-dividing cells (in G0) to enter division can be studied using primary cultures of lymphocytes. The cells are stable in G0 unless stimulated by mitogen such as concanavalin A (Con A). Commitment to enter the division cycle depends on the expression of gene(s) induced by Con A and the synthesis of ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/310143a0

    authors: Milner J

    更新日期:1984-07-12 00:00:00

  • Author Correction: Attosecond angular streaking and tunnelling time in atomic hydrogen.

    abstract::In this Letter, the statement 'I.I. and A.B. performed computations at the NCI Australia' was missing from the Acknowledgements section. This has been corrected online. ...

    journal_title:Nature

    pub_type: 已发布勘误

    doi:10.1038/s41586-019-1123-5

    authors: Sainadh US,Xu H,Wang X,Atia-Tul-Noor A,Wallace WC,Douguet N,Bray A,Ivanov I,Bartschat K,Kheifets A,Sang RT,Litvinyuk IV

    更新日期:2019-05-01 00:00:00

  • Modelling the long QT syndrome with induced pluripotent stem cells.

    abstract::The ability to generate patient-specific human induced pluripotent stem cells (iPSCs) offers a new paradigm for modelling human disease and for individualizing drug testing. Congenital long QT syndrome (LQTS) is a familial arrhythmogenic syndrome characterized by abnormal ion channel function and sudden cardiac death....

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature09747

    authors: Itzhaki I,Maizels L,Huber I,Zwi-Dantsis L,Caspi O,Winterstern A,Feldman O,Gepstein A,Arbel G,Hammerman H,Boulos M,Gepstein L

    更新日期:2011-03-10 00:00:00

  • Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein.

    abstract::Derivatives of the yeast GCN4 transcription factor containing acidic regions of 35 to 40 amino acids fused directly to the DNA-binding domain are fully functional in vivo. High resolution deletion analysis and proteolytic mapping suggest that the activation region is a repeated structure composed of small units acting...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/333635a0

    authors: Hope IA,Mahadevan S,Struhl K

    更新日期:1988-06-16 00:00:00

  • An intermediate-mass black hole of over 500 solar masses in the galaxy ESO 243-49.

    abstract::Ultraluminous X-ray sources are extragalactic objects located outside the nucleus of the host galaxy with bolometric luminosities exceeding 10(39) erg s(-1). These extreme luminosities-if the emission is isotropic and below the theoretical (Eddington) limit, where the radiation pressure is balanced by the gravitationa...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature08083

    authors: Farrell SA,Webb NA,Barret D,Godet O,Rodrigues JM

    更新日期:2009-07-02 00:00:00

  • MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs.

    abstract::Herpesviruses are characterized by their ability to maintain life-long latent infections in their animal hosts. However, the mechanisms that allow establishment and maintenance of the latent state remain poorly understood. Herpes simplex virus 1 (HSV-1) establishes latency in neurons of sensory ganglia, where the only...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature07103

    authors: Umbach JL,Kramer MF,Jurak I,Karnowski HW,Coen DM,Cullen BR

    更新日期:2008-08-07 00:00:00

  • Measurement of a confinement induced neutron phase.

    abstract::Particle physicists see neutrons as tiny massive particles with a confinement radius of about 0.7 fm and a distinct internal quark gluon structure. In quantum mechanics, neutrons are described by wave packets whose spatial extent may become ten orders of magnitude larger than the confinement radius, and can even reach...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature00773

    authors: Rauch H,Lemmel H,Baron M,Loidl R

    更新日期:2002-06-06 00:00:00

  • Extensive variation of human immunodeficiency virus type-1 in vivo.

    abstract::Genotypic variation among independent isolates of human immunodeficiency virus type-1 (HIV-1) is well known, but its molecular basis and biological consequences are poorly understood. We examined the genesis of molecular variation in HIV-1 by sequential virus isolations from two chronically infected individuals and an...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/334440a0

    authors: Saag MS,Hahn BH,Gibbons J,Li Y,Parks ES,Parks WP,Shaw GM

    更新日期:1988-08-04 00:00:00

  • TFIIIC relieves repression of U6 snRNA transcription by chromatin.

    abstract::The U6 small nuclear (sn)RNA gene (SNR6) from the yeast Saccharomyces cerevisiae is transcribed by RNA polymerase III in vivo. This gene is unusual in having a TATA box at position -30, and an essential B-block element located downstream of the T-rich termination signal. The B block is one of the two intragenic promot...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/362475a0

    authors: Burnol AF,Margottin F,Huet J,Almouzni G,Prioleau MN,Méchali M,Sentenac A

    更新日期:1993-04-01 00:00:00