Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Aspidosperma excelsum Benth. (Apocynaceae), a native tree in the Brazilian Amazonia, is traditionally used to treat various diseases, including malaria. AIM OF STUDY:To investigate the chemical constitution, antiplasmodial activity and cytotoxicity of samples obtained from A. excelsum trunk bark by different procedures aiming to evaluate their potential as an antimalarial phytomedicine. MATERIALS AND METHODS:A hydroethanolic extract and alkaloid extracts were prepared and assayed for antiplasmodial activity and cytotoxicity against chloroquine-resistant Plasmodium falciparum (W2 strain) and HepG2 cells, respectively. Taking into account the known occurrence and antimalarial activity of Aspidosperma monoterpene indole alkaloids (MIA), acid-base extractions were carried out and the fractions were assayed for antiplasmodial activity and cytotoxicity. All the samples were analysed by hyphenated chromatographic techniques, such as UPLC-DAD-ESI-MS/MS and HRMS (HPLC-MS MicroTOF), comparing their chemical composition to the literature data. RESULTS:The hydroethanolic extract disclosed a moderate in vitro activity against chloroquine-resistant Plasmodium falciparum (W2 strain) with IC50 23.68 ± 3.08 µg/mL), low cytotoxicity to HepG2 cells (> 250 µg/mL) and good SI (> 10.56). A total of 20 known monoterpene indole alkaloids were identified, seven of which are here firstly described for A. excelsum. Known highly active alkaloids, namely demethylaspidospermine, aspidocarpine, and ochrolifuanine are present in active alkaloid fractions and might contribute to their observed antiplasmodial effect. An alkaloid fraction (Ae-Alk2), obtained directly from trunk bark by extraction with dil. aqueous HCl, pointed out for its activity (IC50 8.75±2.26 µg/mL, CC50 185.14±1.97 µg/mL, SI 21.16) and should be highlighted as the most promising out of the assayed samples. CONCLUSION:The present results represent a preliminary support to the alleged antimalarial use of A. excelsum trunk bark and allowed to highlight alkaloid fractions as promising phytomedicines.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
do Nascimento MS,Pina NDPV,da Silva ASB,Gomes LFDS,de Vasconcellos F,Brandão GC,do Nascimento MFA,de Oliveira AB,Barbosa WLRdoi
10.1016/j.jep.2018.09.012subject
Has Abstractpub_date
2019-01-10 00:00:00pages
99-109eissn
0378-8741issn
1872-7573pii
S0378-8741(18)31601-5journal_volume
228pub_type
杂志文章abstract:ETHNOPHARMACOLOGICAL RELEVANCE:In Brazil, latex of Himatanthus drasticus is used to treat inflammation, wound healing and cancer. The present study evaluated the antitumoral potential of H. drasticus latex (HdCL) in Sarcoma 180-bearing mice (S180). MATERIALS AND METHODS:HdCL was obtained in Crato-CE, Brazil. Qualitati...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.09.043
更新日期:2018-06-28 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Banisteriopsis caapi, a woody vine from the Amazonian basin, is popularly known as an ingredient of a sacred drink ayahuasca, widely used throughout the Amazon as a medicinal tea for healing and spiritual exploration. The usefulness of Banisteriopsis caapi has been established for allevia...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.02.013
更新日期:2010-04-21 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Damiana (Turnera diffusa Willd. Ex Schult) has traditionally been used as an herbal aphrodisiac. AIM OF THE STUDY:The study was aimed to investigate the anti-aromatse activity and the estrogenic activity of the constituents isolated from Turnera diffusa. MATERIALS AND METHODS:The methan...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.09.016
更新日期:2008-12-08 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.01.030
更新日期:2018-04-24 00:00:00
abstract::This paper discusses the use of animals prescribed as medicines by herbalists from Feira de Santana city in the State of Bahia, Northeastern Brazil. Data were obtained by undergraduates of the Biology course of Feira de Santana State University, who performed open interviews with herbalists at Centro de Abastecimento,...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.09.018
更新日期:2016-11-04 00:00:00
abstract::In the biological sciences the use of medicinal plants in indigenous cultures is commonly seen as being based on a long tradition ('traditional medicine'). However, under normal circumstances, ethnobotanical studies cannot provide evidence on the antiquity of specific uses for medicinal plants since oral traditions ha...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/s0378-8741(03)00188-0
更新日期:2003-10-01 00:00:00
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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更新日期:2015-06-20 00:00:00
abstract::We evaluate the mode of action of verbascoside obtained from Buddleja cordata against Staphylococcus aureus by killing kinetics and incorporation of precursors methods. Verbascoside induced lethal effect on S. aureus, by affecting protein synthesis and inhibiting leucine incorporation. ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(98)00203-7
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abstract::Shuang-Huang-Lian (SHL) is a traditional Chinese formula containing Lonicerae japonicae flos (LJF), Scutellariae radix (SR) and Forsythiae fructus (FF), and is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. The aim of this study is to compare the pharmacokinetics ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.03.030
更新日期:2006-10-11 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:As a well-known traditional Chinese medicine the root bark of Aralia taibaiensis has traditionally been used as the medicine considered alleviating several disorders including diabetes mellitus (DM). Chikusetsu saponin IVa (CHS) has been defined as a major active ingredient of triterpenoi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.02.032
更新日期:2015-04-22 00:00:00
abstract::The aqueous extract of Nigella sativa (N. sativa) was investigated for anti-inflammatory, analgesic and antipyretic activities in animal models. The extract has an anti-inflammatory effect demonstrated by its inhibitory effects on Carrageenan induced paw edema. It also produced significant increase in the hot plate re...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.01.036
更新日期:2005-04-08 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(88)90124-9
更新日期:1988-02-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2014-05-14 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00037-0
更新日期:2003-06-01 00:00:00
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2015-08-22 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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更新日期:2019-01-30 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.07.038
更新日期:2014-09-29 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(92)90025-m
更新日期:1992-01-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2019-12-05 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2018-06-12 00:00:00
abstract::The effects of cryptolepine alone and in combination with other antiplatelet agents have been investigated using a mouse model of arterial thrombosis. Intraperitoneal premedication with crytolepine produced 25% maximal protection at 1 mg/kg while dipyridamole producedd a 20% maximal effect at 2 mg/kg. Higher doses of ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(89)90086-x
更新日期:1989-11-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.05.065
更新日期:2011-07-14 00:00:00
abstract::A rapid 96-well plate assay using sulphorhodamine B (SRB) protein stain for cell number has been adopted to screen herbs used in traditional treatments of vitiligo for substances capable of stimulating melanocyte proliferation. Its applicability to melan-a cells, a mouse pigmented cell line, has been validated. SRB as...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(98)00199-8
更新日期:1999-08-01 00:00:00
abstract::Dilution methods were employed to determine the effect of ethanolic extracts of Agave lecheguilla Torr. (Agavaceae), Baccharis glutinosa Pers. (Compositae) and Larrea tridentata (DC.) Cov. (Zygophyllaceae) on growth of yeasts, molds and bacteria. The three extracts analyzed showed good antimicrobial activity against m...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:1996-07-05 00:00:00