Abstract：
:We demonstrated the presence of specific binding sites for bullfrog luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in a testicular crude plasma membrane fraction of the bullfrog, Rana catesbeiana. The equilibrium analysis of the binding showed that a straight line was fitted to the Scatchard plot of LH and a curvilinear line to that of FSH, suggesting the presence of single-type binding sites for LH and the presence of multiple-type binding sites or negative cooperactivity of the binding for FSH. Competition experiments showed that bullfrog LH could replace the specific binding of bullfrog FSH and vice versa. Binding affinity of bullfrog LH to radioiodinated bullfrog FSH binding sites was as high as that of intact bullfrog FSH to the same sites. In contrast, binding properties of bullfrog FSH to radioiodinated bullfrog LH binding sites were not simple: the competition curve obtained with FSH against the binding of bullfrog LH had an extremely low slope value and was not parallel to that obtained with LH. These results suggest that differentiation of LH and FSH receptors is not complete in this species, although it has been reported that these hormones have separate actions on the testis. Competition experiments further showed that bullfrog testicular LH and FSH receptors possessed higher affinities for gonadotrophins of homologous or closely related animal species compared with phylogenically distant groups. Species specificity of the hormone-receptor interaction may cause species specificity of the gonadotrophin action.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Takada K,Kubokawa K,Ishii Sdoi
10.1016/0016-6480(86)90207-8subject
Has Abstractpub_date
1986-02-01 00:00:00pages
302-12issue
2eissn
0016-6480issn
1095-6840journal_volume
61pub_type
杂志文章abstract：:Normal saline, L-thyroxine (L-T4, 0.5 micrograms/bird/day), and testosterone (100 micrograms/bird/day) were administered separately to intact, thyroidectomized, and castrated spotted munia over a 30-day period and the effects on basal metabolic rate (BMR), gonads, and body weight were recorded. Thyroidectomy and castr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90062-5
更新日期：1984-10-01 00:00:00
abstract：:Birds respond to environmental changes by modulating their levels of plasma corticosterone, a stress hormone produced by the adrenal glands. Baseline levels of corticosterone and the magnitude of adrenocortical response to acute stress are known to vary seasonally. Photoperiod is one of several potential factors which...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2002.7798
更新日期：2002-04-01 00:00:00
abstract：:1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the hormonally active metabolite of vitamin D3 interacts with its nuclear/cytosolic receptor to induce biological responses in target tissues. Naked mole rats appear to be naturally deficient in vitamin D. The questions arise whether these animals possess the 1,25(OH)2D3 recepto...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1089
更新日期：1993-06-01 00:00:00
abstract：:Y-Organs and control tissues from intact (intermolt) and 48-hr de-eyestalked Cancer antennarius donors were cultured for 12 and 24 hr in crustacean saline supplemented 10% with crab serum and containing [14C]cholesterol. Under these conditions, Y-organs took up significantly more [14C]cholesterol than ovary or muscle,...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90215-1
更新日期：1985-03-01 00:00:00
abstract：:Interference in radioimmunoassays (RIAs) was frequently encountered during endocrinological studies of the emu (Dromaius novaehollandiae). Interference was greatest when serum was cloudy or opaque. Such samples appeared seasonally, in spring and summer during the phase of fat deposition, and in the winter when females...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00088-1
更新日期：2003-06-15 00:00:00
abstract：:A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 +/- 14 nM. The caudal neurosecretory peptide urotensin II at 10(-7) to 10(-6)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90068-7
更新日期：1986-12-01 00:00:00
abstract：:Circannual and circadian variations in plasma levels of steroids were estimated by radioimmunoassay in the female and male catfish, Heteropneustes fossilis, over two consecutive annual reproductive cycles. In the female catfish, testosterone (T), estradiol-17 beta (E2), and estrone (E1) were detectable in the plasma o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90221-6
更新日期：1983-05-01 00:00:00
abstract：:The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pub...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113656
更新日期：2021-01-15 00:00:00
abstract：:In 1974, a lack of 5α-dihydrotestosterone (5α-DHT), the most potent androgen across species except for fish, was shown to be the origin of a type of pseudohermaphrodism in which boys have female-like external genitalia. This human intersex condition is linked to a mutation in the steroid-5α-reductase type 2 (SRD5α2) g...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2020.113400
更新日期：2020-05-01 00:00:00
abstract：:Orexin A (OXA), a hypothalamic neuropeptide, and its receptor (OX1R) are primarily expressed in lateral hypothalamus and are involved in the control of various biological functions. Expressions of OXA and OX1R have also been reported in peripheral organs like gastrointestinal and genital tracts. In the present study, ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.11.005
更新日期：2016-12-01 00:00:00
abstract：:Seasonally-breeding amphibians have served as excellent animal models to investigate the biosynthesis and biological actions of neurosteroids. Previous studies have demonstrated that the brain of amphibians possesses key steroidogenic enzymes and produces pregnenolone, a precursor of steroid hormones, and other variou...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2011.11.020
更新日期：2012-05-01 00:00:00
abstract：:Female American tree sparrows (Spizella arborea) were injected with Na131I before, at, or after the onset of photostimulation in order to study the effect of time of thyroidectomy on three components of seasonality: thyroid-dependent photoperiodic ovarian growth, photorefractoriness, and postnuptial (prebasic) molt. T...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7267
更新日期：1999-06-01 00:00:00
abstract：:In songbirds, neurons that regulate learned song behavior undergo extensive seasonal plasticity in their number and size in relation to the bird's reproductive status. Seasonal plasticity of these brain regions is primarily regulated by changes in circulating concentrations of testosterone. Androgen receptors are pres...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.08.001
更新日期：2010-03-01 00:00:00
abstract：:Two aspects of hormonal control of aggressive territorial behavior in male mountain spiny lizards Sceloporus jarrovi were studied. First, testosterone (T) implants were given to free-living castrated males during the breeding season. These implants fully restored breeding season levels of aggressive and sexual behavio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90121-9
更新日期：1988-06-01 00:00:00
abstract：:Although present at relatively low number in the testis, spermatogonial stem cells (SSCs) are crucial for the establishment and maintenance of spermatogenesis in eukaryotes and, until recently, those cells were investigated in fish using morphological criteria. The isolation and characterization of these cells in fish...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2014.06.018
更新日期：2014-10-01 00:00:00
abstract：:In Sphaeroma serratum, the differentiation of the male external sexual characteristics, as a result of an androgenic gland implant, proceeds more easily in females in vitellogenesis than in immature females. On the contrary, the transformation of the gonads is quicker and more obvious in immature females than in matur...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90250-2
更新日期：1983-04-01 00:00:00
abstract：:Effects of Al on estradiol-induced vitellogenin (VTG) induction were electrophoretically examined in primary hepatocyte cultures in rainbow trout. Hepatocytes were precultured for 2 days and then estradiol-17 beta (E2, 6 x 10(-6) M) and Al (10(-6)-10(-4) M) were added to the incubation medium. The hepatocytes were cul...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6996
更新日期：1998-01-01 00:00:00
abstract：:The present study in seasonally breeding spotted snakehead Channa punctatus, for the first time in nonmammalian vertebrates, demonstrated correlation between reproductive phase-dependent testicular expression of ligand Jag1/receptor Notch1 and spermatogenic events. Testicular transcriptome sequencing data from our ear...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.05.014
更新日期：2018-09-15 00:00:00
abstract：:Seasonal patterns of fecal 17beta-OH-androgen, estrogen, and progesterone equivalents of male and female greylag geese (Anser anser) were analyzed in a flock of free-living geese. These were compared among social categories determined by pairbond status and breeding success. The annual cycle was divided into 13 phases...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7236
更新日期：1999-04-01 00:00:00
abstract：:The presence and distribution of cholecystokinin (CCK)/gastrin-like immunoreactivity (IR) was examined in the goldfish pituitary. Intense CCK/gastrin-like IR was consistently observed within fibers of the proximal pars distalis (PD), with fewer IR fibers localized in the rostral PD. Within the proximal PD CCK/gastrin-...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1146
更新日期：1993-10-01 00:00:00
abstract：:The G-protein-coupled receptor 54 (muGPR54) cDNA was cloned from the brain of the grey mullet, and its expression level, as well as those of the gonadotropin-releasing hormones (GnRH1, GnRH2, GnRH3) and dopamine receptor D2 (drd2), in the brain, pituitary and ovary of pubertal fish (early, intermediate, advanced) were...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.09.008
更新日期：2007-01-15 00:00:00
abstract：:A detailed study was made of sex differentiation in three anteroposteriorly distributed body regions of the long gonadal fold in young Pacific hagfish (Eptatretus stouti) from 12.4 to 45 cm in length. In general, the posterior region, adjacent to the cloaca, remained undifferentiated, and only in specimens more than 2...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90315-d
更新日期：1990-02-01 00:00:00
abstract：:The insulin-like growth factor-1 (IGF-1) plays an important role in the regulation of reproductive function. In the present study, we examined the effects of caloric restriction (CR) on the reproductive lifespan in rats and investigated the potential role of IGF-1. After 10 weeks of treatment, we determined the distri...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.09.005
更新日期：2011-11-01 00:00:00
abstract：:Two forms of immunoreactive gonadotropin-releasing hormone (GnRH) were extracted from brain-pituitary tissues of Thai catfish, Clarias macrocephalus and C. batrachus. The peptides were detected using high performance liquid chromatography (HPLC) and radioimmunoassay (RIA). In both the HPLC systems, catfish GnRH-I elut...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90031-e
更新日期：1992-08-01 00:00:00
abstract：:Experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench Tinca tinca and goldfish Carassius auratus and compare it to cryopreservation of isolated testicular cells. Additionally, effects of three cryoprotectants (dimethyl sulphoxyde - Me2SO, methanol - MeOH and ethyle...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.07.005
更新日期：2017-05-01 00:00:00
abstract：:The objective of this study was to examine if RU486, a corticosteroid antagonist, modified hepatocyte metabolism and blocked the metabolic effects of exogenously administered cortisol in rainbow trout (Oncorhynchus mykiss). The fish were given a single intraperitoneal implant of either coconut oil alone or coconut oil...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1160
更新日期：1994-10-01 00:00:00
abstract：:The effects of fasting and feeding on the brain expression of orexin (OX), tyrosine hydroxylase (TH), peptide Y (PY) and cholecystokinin (CCK) were examined in the blind cavefish Astyanax fasciatus mexicanus. A 10-days fasting period induced increases in both OX and TH brain mRNA expression but had no effect on PYY an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.12.011
更新日期：2013-03-01 00:00:00
abstract：:In the domestic fowl, plasma concentrations of growth hormone (GH) have been observed to be elevated in young, rapidly growing chicks and low in older chicks and in adults. In this present study the plasma concentration of GH was high in 35-day-old male chickens and declined to the low adult concentration by 91 days o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90268-5
更新日期：1984-03-01 00:00:00
abstract：:Migratory birds undergo metabolic remodeling in tissues, including increased lipid storage in white adipose and fatty acid uptake and oxidation in skeletal muscle, to optimize energy substrate availability and utilization in preparation for long-distance flight. Different tissues undergo gene expression changes in kee...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.08.009
更新日期：2018-11-01 00:00:00
abstract：:By mapping the distribution of cholecystokinin (CCK) receptor types onto an established phylogenetic hypothesis of vertebrate relationships, we tested two hypothesis about the evolution of CCK receptors: (1) A single CCK receptor type, CCK-X, is the ancestral receptor, while CCK-A and CCK-B receptors represent derived...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0047
更新日期：1996-04-01 00:00:00
