Detection of common diarrhea-causing pathogens in Northern Taiwan by multiplex polymerase chain reaction.

Abstract:

:Conventional methods for identifying gastroenteritis pathogens are time consuming, more likely to result in a false-negative, rely on personnel with diagnostic expertise, and are dependent on the specimen status. Alternatively, molecular diagnostic methods permit the rapid, simultaneous detection of multiple pathogens with high sensitivity and specificity. The present study compared conventional methods with the Luminex xTAG Gastrointestinal Pathogen Panel (xTAG GPP) for the diagnosis of infectious gastroenteritis in northern Taiwan. From July 2015 to April 2016, 217 clinical fecal samples were collected from patients with suspected infectious gastroenteritis. All specimens were tested using conventional diagnostic techniques following physicians' orders as well as with the xTAG GPP. The multiplex polymerase chain reaction (PCR) approach detected significantly more positive samples with bacterial, viral, and/or parasitic infections as compared to conventional analysis (55.8% vs 40.1%, respectively; P < .001). Moreover, multiplex PCR could detect Escherichia coli O157, enterotoxigenic E coli, Shiga-like toxin-producing E coli, Cryptosporidium, and Giardia, which were undetectable by conventional methods. Furthermore, 48 pathogens in 23 patients (10.6%) with coinfections were identified only using the multiplex PCR approach. Of which, 82.6% were from pediatric patients. Because the detection rates using multiplex PCR are higher than conventional methods, and some pediatric pathogens could only be detected by multiplex PCR, this approach may be useful in rapidly diagnosing diarrheal disease in children and facilitating treatment initiation. Further studies are necessary to determine if multiplex PCR improves patient outcomes and reduces costs.

journal_name

Medicine (Baltimore)

journal_title

Medicine

authors

Huang SH,Lin YF,Tsai MH,Yang S,Liao ML,Chao SW,Hwang CC

doi

10.1097/MD.0000000000011006

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

e11006

issue

23

eissn

0025-7974

issn

1536-5964

pii

00005792-201806080-00050

journal_volume

97

pub_type

杂志文章

相关文献

MEDICINE文献大全
  • Expanding pancreas donor pool by evaluation of unallocated organs after brain death: Study protocol clinical trial (SPIRIT Compliant).

    abstract:BACKGROUND:Pancreas graft quality directly affects morbidity and mortality rates after pancreas transplantation (PTx). The criteria for pancreas graft allocation are restricted, which has decreased the number of available organs. Suitable pancreatic allografts are selected based on donor demographics, medical history, ...

    journal_title:Medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/MD.0000000000019335

    authors: Kulu Y,Khajeh E,Ghamarnejad O,Nikdad M,Sabagh M,Ali-Hasan-Al-Saegh S,Nadalin S,Quante M,Pisarski P,Jänigen B,Reißfelder C,Mieth M,Morath C,Goeppert B,Schirmacher P,Strobel O,Hackert T,Zeier M,Springel R,Schleicher C

    更新日期:2020-03-01 00:00:00

  • A cross-sectional study investigating the relationship between handgrip strength with multimorbidity in Korean adults: Findings from the Korea National Health and Nutrition Examination Survey VI-VII (2014-2017).

    abstract:ABSTRACT:To date research investigating the associations between handgrip strength and multimorbidity when stratifying by age and sex is limited. Furthermore, this is the first study in a Korean population, and the first to include adults ≥ 19 years of age. Here we investigated the associations between handgrip strengt...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000023994

    authors: Lee MR,Jung SM

    更新日期:2021-01-08 00:00:00

  • Effective treatment using itraconazole combined with terbinafine in the treatment of nasal sporotrichosis: A case report.

    abstract:RATIONALE:Sporotrichosis is a subacute or chronic infection caused by sporothrix schenckii complex. The misdiagnosis rate of sporotrichosis is very high. Fungal microscopic examination and timely culture help us make an accurate diagnosis and treatment. We observed that combined treatments are more effective than monot...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000017155

    authors: Guo K,Wang S,Wang Z,Zhang L

    更新日期:2019-09-01 00:00:00

  • Clinical analysis of hepatic angioleiomyoma: Two case reports.

    abstract:RATIONALE:Angioleiomyoma is an uncommon benign tumor that originates from the vascular smooth muscle cells and contains thick-walled vessels. It can appear anywhere in the body but more frequently in the extremities (especially in the lower limbs) and rarely invades the internal organs. PATIENT CONCERNS:A 52-year-old ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000014661

    authors: Jiang B,Chen QN,Qi FZ,Xu JB,Yu YB,Song Y

    更新日期:2019-03-01 00:00:00

  • Milk and yogurt intake and breast cancer risk: A meta-analysis.

    abstract::Different patterns of milk and breast cancer rates across countries suggest that several components of milk could affect breast cancer risk. However, the components of diet are complex including milk that could potentially influence risk. Some milk products such as whole milk and cheese have a high fat content which m...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000014900

    authors: Chen L,Li M,Li H

    更新日期:2019-03-01 00:00:00

  • The association between rs12807809 polymorphism in neurogranin gene and risk of schizophrenia: A meta-analysis.

    abstract:BACKGROUND:The correlation between single nucleotide polymorphism (SNP) rs12807809 in Neurogranin (NRGN) gene and Schizophrenia (SCZ) was investigated by several studies, whereas the results were conflicting. Thus, we performed the present meta-analysis to combine and analyze the available studies in order to provide a...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000018518

    authors: Jin L,An Z,Xu B,Mu D,Fu S,Hu H,Shi Y,Luo X,Yi Q

    更新日期:2019-12-01 00:00:00

  • Point prevalence and epidemiological characteristics of chronic cough in the general adult population: The Korean National Health and Nutrition Examination Survey 2010-2012.

    abstract::Cough is frequently self-limiting, but may persist longer in certain individuals. Most of previous studies on the epidemiology of chronic cough have only measured period prevalence, and thus have afforded limited information on the burden and natural course. We aimed to investigate the epidemiology of chronic cough by...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000006486

    authors: Kang MG,Song WJ,Kim HJ,Won HK,Sohn KH,Kang SY,Jo EJ,Kim MH,Kim SH,Kim SH,Park HW,Chang YS,Lee BJ,Morice AH,Cho SH

    更新日期:2017-03-01 00:00:00

  • The role of IL22 polymorphisms on liver cirrhosis in patients with hepatitis B virus: A case control study.

    abstract:AIMS:Interleukin(IL)-22 plays an important role in promoting liver regeneration and repair, but its role in chronic HBV-related liver diseasesis not clear. The goal of this study was to evaluate associations between eight IL22 single nucleotide polymorphisms (SNPs) and the development of chronic HBV cirrhosis and HBV-r...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000017867

    authors: Gao YH,Li QQ,Wang CG,Sun J,Wang XM,Li YJ,He XT,Xu HQ,Niu JQ

    更新日期:2019-11-01 00:00:00

  • Laboratory epidemiology of respiratory viruses in a large children's hospital: A STROBE-compliant article.

    abstract::Acute respiratory tract infection (ARTI) is the most common causes of outpatient visit and hospital admission for children. The study aimed to report epidemiological data on respiratory viruses in a university-affiliated children's hospital.The study was a retrospective study conducted in a university affiliated child...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000011385

    authors: Ye S,Wang T

    更新日期:2018-07-01 00:00:00

  • Efficacy and safety of dual filtration plasmapheresis combined with biological agents in active refractory rheumatod arthritis: A retrospective cohort study.

    abstract::To investigate the effectiveness of dual filtration plasmapheresis (DFPP), a novel blood purification treatment, as a rapid and sustained disease-modifying therapy for active refractory rheumatoid arthritis (RA).A retrospective cohort study had been conducted. One hundred fifty three patients aged 18 years or older wi...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000020966

    authors: Xing Y,Wang S,Liu C,Wang H,Zhao M,Jin B,Kong X

    更新日期:2020-07-10 00:00:00

  • Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells.

    abstract::Xiakemycin A (XKA), a new antibiotic in the pyranonaphthoquinone family, shows antitumor activity. However, the type of cell death induced by XKA remains elusive. In this study, we aim to investigate the type of death induced by XKA in hepatic cancer.The apoptotic features, such as chromatic agglutination, reactive ox...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000019848

    authors: Chen C,Han Z,Yang M,Jiang Z,Ou X

    更新日期:2020-04-01 00:00:00

  • Imaging findings of intraductal tubulopapillary neoplasm (ITPN) of the pancreas: Two case reports and literature review.

    abstract:RATIONALE:Intraductal tubulopapillary neoplasm (ITPN) is a rare type of pancreatic epithelial neoplasm. We report 2 cases of ITPN and detail the imaging findings. PATIENT CONCERNS:The 1st case was a 36-year-old woman who complained of jaundice, yellow urine and diarrhea. She accepted ultrasound, computed tomography (C...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000014426

    authors: Zhang J,Ren S,Wang J,Ye D,Zhang H,Qiu W,Wang Z

    更新日期:2019-02-01 00:00:00

  • Age, sex, and the association of chronic kidney disease with all-cause mortality in Buddhist priests: An analysis of the standardized mortality ratio from the Korean Buddhist priests cohort.

    abstract::Buddhist priests lead a unique lifestyle, practicing asceticism, with a vegetarian diet. Such behavior may have an impact on clinical outcomes. Hence, we explored the mortality among Korean Buddhist priests as compared with the general population.This study is a single-center, retrospective study. Among the 3867 Buddh...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000013099

    authors: Kim HJ,Kim Y,Kim S,Chin HJ,Lee H,Lee JP,Kim DK,Oh KH,Joo KW,Kim YS,Nah DY,Shin SJ,Kim KS,Park JY,Yoo KD

    更新日期:2018-11-01 00:00:00

  • Successful superselective embolization with n-butyl cyanoacrylate for hemorrhage from superficial femoral artery branches following blunt trauma: Three case reports.

    abstract:INTRODUCTION:In blunt traumatic superficial femoral arterial (SFA) injuries, hemorrhage from the branches without injury to the main artery is rare, but can lead to serious complications, such as compartment syndrome affecting the clinical outcomes. Although open surgical repair has been the standard approach to periph...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000020467

    authors: Oh CH,Kim Y,Lee JH,Kim HR,Go SJ

    更新日期:2020-07-02 00:00:00

  • Prognostic Value of KIF2A and HER2-Neu Overexpression in Patients With Epithelial Ovarian Cancer.

    abstract::Kinesin family member 2A (KIF2A) is a member of Kinesin-13 family and involved in cell migration and cell signaling. Human epidermal growth factor receptor 2 (HER2-neu) is implicated in the development of many cancers. Both of these 2 proteins are upstream inducer of PI3K/AKT signaling pathway that plays an important ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000002803

    authors: Wang D,Zhu H,Ye Q,Wang C,Xu Y

    更新日期:2016-02-01 00:00:00

  • Contrast-enhanced ultrasound for determining muscular perfusion after oral intake of L-citrulline, L-arginine, and galloylated epicatechines: A study protocol.

    abstract:INTRODUCTION:The market for dietary supplements in the sports sector has been growing rapidly for several years, though there is still lacking evidence regarding their claimed benefits. One group is that of nitric oxide increasing supplements, so-called "NO-boosters," which are claimed to improve the supply of oxygen a...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000022318

    authors: Doll J,Bürkle F,Neide A,Tsitlakidis S,Bruckner T,Schmidmaier G,Fischer C

    更新日期:2020-10-09 00:00:00

  • A novel index including SNPs for the screening of nonalcoholic fatty liver disease among elder Chinese: A population-based study.

    abstract::Presently noninvasive methods were employed to the diagnosis of nonalcoholic fatty liver disease (NAFLD), including fatty liver index (FLI), hepatic steatosis index (HSI), product of fasting triglyceride and glucose levels (TyG), and single nucleotide polymorphism (SNP), whereas the accuracy of those indexes need to b...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000010272

    authors: Yang H,Chen G,Song C,Li D,Ma Q,Chen G,Li X

    更新日期:2018-03-01 00:00:00

  • Echinococcosis in left ventricle: a case report.

    abstract:INTRODUCTION:Echinococcosis, also called hydatid disease, is a common parasitic infection of the liver. However, echinococcus lesions rarely involve the heart, especially in children. PATIENT CONCERNS:An 8-year-old child from grazing areas of northwest China was referred to our hospital for the complaint of inpersiste...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000015267

    authors: Su L,Yu J,Dai C,Liu Y,Peng L

    更新日期:2019-04-01 00:00:00

  • Cardiac malignant peripheral nerve sheath tumor on computed tomography and magnetic resonance imaging: A case report.

    abstract:RATIONALE:Malignant peripheral nerve sheath tumor (MPNST) is a very rare sarcoma of the heart, and few cases have been reported. Herein, we retrospectively reviewed clinical manifestations, imaging features and management of our patient and other reported cases. PATIENT CONCERNS:A 32-year-old woman was referred to the...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000017463

    authors: Li S,Qiu Y,Yu J,Liang C,Peng L

    更新日期:2019-11-01 00:00:00

  • Strategic use of dual regimens of boosted protease inhibitors plus maraviroc in poorly adherent subjects in view of long-acting drugs: A retrospective study.

    abstract::In view of the forthcoming long-acting antiretrovirals, measures should be taken to prevent the selection of HIV drug resistance mutations. All subjects who had been switched to boosted protease inhibitors plus maraviroc (bPIs/MVC) with baseline HIV-1 RNA >50 copies/mL between June, 2014, and April, 2015, were retrosp...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000005728

    authors: Capetti AF,Micale M,Carenzi L,Niero F,Landonio S,Vimercati S,Dedivitiis G,Rizzardini G

    更新日期:2017-02-01 00:00:00

  • A novel approach for medical research on lymphomas: A study validation of claims-based algorithms to identify incident cases.

    abstract::The use of claims database to study lymphomas in real-life conditions is a crucial issue in the future. In this way, it is essential to develop validated algorithms for the identification of lymphomas in these databases. The aim of this study was to assess the validity of diagnosis codes in the French health insurance...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000009418

    authors: Conte C,Palmaro A,Grosclaude P,Daubisse-Marliac L,Despas F,Lapeyre-Mestre M

    更新日期:2018-01-01 00:00:00

  • The role of real-time elastography-targeted biopsy in the detection and diagnosis of prostate cancer: A systematic review and meta-analysis.

    abstract:BACKGROUND:The role of real-time elastography (RTE)-targeted biopsy in the detection and diagnosis of prostate cancer (PCa) remains controversial. METHODS:We searched Medline, Embase, and Cochrane Library from inception to July 31, 2017 and used the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool to ...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析,评审

    doi:10.1097/MD.0000000000010220

    authors: Tu X,Qiu S,Chang T,Jin K,Bao Y,Yang L,Wei Q

    更新日期:2018-03-01 00:00:00

  • Conservative treatment of patients with bladder genital tract fistula: Three case reports.

    abstract:INTRODUCTION:Most of the patients with bladder genital tract fistula recover with surgical treatment. In the present study, we aimed to assess conservative treatment strategies for bladder genital tract fistula. PATIENT CONCERNS:We reviewed 3 cases with bladder genital tract fistula who underwent treatment at our hosp...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000021430

    authors: He Z,Cui L,Wang J,Gong F,Jia G

    更新日期:2020-07-31 00:00:00

  • Clinical significance of myositis-specific autoantibody profiles in Japanese patients with polymyositis/dermatomyositis.

    abstract::Myositis-specific autoantibodies, such as anti-melanoma differentiation associated gene 5 (MDA5) and anti-anti-amino acyl-tRNA synthetases (ARS) antibodies, are associated with interstitial lung diseases (ILD), which determine the prognosis of polymyositis/dermatomyositis (PM/DM) patients. However, there is a paucity ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000015578

    authors: Temmoku J,Sato S,Fujita Y,Asano T,Suzuki E,Kanno T,Furuya MY,Matsuoka N,Kobayashi H,Watanabe H,Koga T,Shimizu T,Kawakami A,Migita K

    更新日期:2019-05-01 00:00:00

  • Efficacy of afatinib for pulmonary adenocarcinoma with leptomeningeal metastases harboring an epidermal growth factor receptor complex mutation (exon 19del+K754E): A case report.

    abstract:RATIONALE:Liquid biopsy of cerebrospinal fluid (CSF) and sequencing of cell-free DNA has rarely been used to identify epidermal growth factor receptor (EGFR) mutations, which can guide the design of precise, personalized treatment for patients with leptomeningeal metastasis from lung adenocarcinoma. PATIENT CONCERNS:A...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000022851

    authors: Ma C,Liu M,Mu N,Li J,Li L,Jiang R

    更新日期:2020-10-23 00:00:00

  • Anesthetic management of off-pump simultaneous coronary artery bypass grafting and lobectomy: Case report and literature review.

    abstract:RATIONALE:Survey data show approximately 10% patients with lung cancer may present concomitant coronary heart disease. Simultaneous surgery is a challenge for anesthetist. We review our experience in the anesthesia with 5 patients who required simultaneous off-pump coronary artery bypass grafting (OPCABG) and pulmonary...

    journal_title:Medicine

    pub_type: 杂志文章,评审

    doi:10.1097/MD.0000000000008780

    authors: Zhao X,Li Y,Kong HY,Zhang L,Wen XH

    更新日期:2017-12-01 00:00:00

  • Portal vein/superior mesenteric vein resection in pancreatic cancer treatment in the elderly.

    abstract::There is an increased interest in extending surgical criteria for pancreatic cancer by performing pancreaticoduodenectomy (PD) combined with portal vein (PV) or superior mesenteric vein (SMV) resection and reconstruction for borderline resectable patients. However, whether this procedure suitable for elderly patients ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000007335

    authors: Fang JZ,Lu CD,Wu SD,Huang J,Zhou J

    更新日期:2017-07-01 00:00:00

  • Brain metastases from esophageal cancer: A retrospective analysis of the outcome after surgical resection followed by radiotherapy.

    abstract::Brain metastases deriving from esophageal cancer are very rare with scarce data available concerning these patients' outcome. We, therefore, evaluated outcome after surgical resection followed by radiotherapy of brain metastasis from esophageal cancer.A retrospective analysis was conducted on consecutive patients unde...

    journal_title:Medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/MD.0000000000016653

    authors: Stavrinou P,Plambeck L,Proescholdt M,Ghadimi M,Goldbrunner R,Grau S

    更新日期:2019-08-01 00:00:00

  • Investigation of the interaction between proton pump inhibitors and clopidogrel using VerifyNow P2Y12 assay.

    abstract:BACKGROUND:Randomized trials and observation studies have revealed conflicting results regarding the interaction between clopidogrel and proton pump inhibitors (PPIs). The aim of our study was to provide laboratory evidence regarding whether PPIs blunt the antiplatelet reactivity of clopidogrel. METHODS:We included re...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000023695

    authors: Lin SF,Lin PC,Chang CC,Chang WL,Chu FY

    更新日期:2020-12-11 00:00:00

  • Stereotactic radiotherapy in neovascular age-related macular degeneration: Real-life efficacy and morphological evaluation of the outer retina-choroid complex.

    abstract::Stereotactic radiotherapy (SRT) is a new approach to treat neovascular age-related macular degeneration (nAMD). The INTREPID trial suggested that SRT could reduce the frequency of regular intravitreal injections (IVIs) with antivascular endothelial growth factor drugs, which are necessary to control disease activity. ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000005729

    authors: Ranjbar M,Kurz M,Holzhey A,Melchert C,Rades D,Grisanti S

    更新日期:2016-12-01 00:00:00