Anti-quorum Sensing Activities of Selected Coral Symbiotic Bacterial Extracts From the South China Sea.

Abstract:

:The worldwide increase in antibiotic-resistant pathogens means that identification of alternative antibacterial drug targets and the subsequent development of new treatment strategies are urgently required. One such new target is the quorum sensing (QS) system. Coral microbial consortia harbor an enormous diversity of microbes, and are thus rich sources for isolating novel bioactive and pharmacologically valuable natural products. However, to date, the versatility of their bioactive compounds has not been broadly explored. In this study, about two hundred bacterial colonies were isolated from a coral species (Pocillopora damicornis) and screened for their ability to inhibit QS using the bioreporter strain Chromobacterium violaceum ATCC 12472. Approximately 15% (30 isolates) exhibited anti-QS activity, against the indicator strain. Among them, a typical Gram-positive bacterium, D11 (Staphylococcus hominis) was identified and its anti-QS activity was investigated. Confocal microscopy observations showed that the bacterial extract inhibited the biofilm formation of clinical isolates of wild-type P. aeruginosa PAO1 in a dose-dependent pattern. Chromatographic separation led to the isolation of a potent QS inhibitor that was identified by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and nuclear magnetic resonance (NMR) spectroscopy as DL-homocysteine thiolactone. Gene expression analyses using RT-PCR showed that strain D11 led to a significant down-regulation of QS regulatory genes (lasI, lasR, rhlI, and rhlR), as well as a virulence-related gene (lasB). From the chemical structure, the target compound (DL-homocysteine thiolactone) is an analog of the acyl-homoserine lactones (AHLs), and we presume that DL-homocysteine thiolactone outcompetes AHL in occupying the receptor and thereby inhibiting QS. Whole-genome sequence analysis of S. hominis D11 revealed the presence of predicted genes involved in the biosynthesis of homocysteine thiolactone. This study indicates that coral microbes are a resource bank for developing QS inhibitors and they will facilitate the discovery of new biotechnologically relevant compounds that could be used instead of traditional antibiotics.

authors

Ma ZP,Song Y,Cai ZH,Lin ZJ,Lin GH,Wang Y,Zhou J

doi

10.3389/fcimb.2018.00144

subject

Has Abstract

pub_date

2018-05-08 00:00:00

pages

144

issn

2235-2988

journal_volume

8

pub_type

杂志文章
  • Population Genomics of Francisella tularensis subsp. holarctica and its Implication on the Eco-Epidemiology of Tularemia in Switzerland.

    abstract::Whole genome sequencing (WGS) methods provide new possibilities in the field of molecular epidemiology. This is particularly true for monomorphic organisms where the discriminatory power of traditional methods (e.g., restriction enzyme length polymorphism typing, multi locus sequence typing etc.) is inadequate to eluc...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00089

    authors: Wittwer M,Altpeter E,Pilo P,Gygli SM,Beuret C,Foucault F,Ackermann-Gäumann R,Karrer U,Jacob D,Grunow R,Schürch N

    更新日期:2018-03-22 00:00:00

  • Toward a structural understanding of Clostridium difficile toxins A and B.

    abstract::Clostridium difficile is a toxin-producing bacterium that is a frequent cause of hospital-acquired and antibiotic-associated diarrhea. The incidence, severity, and costs associated with C. difficile associated disease are substantial and increasing, making C. difficile a significant public health concern. The two prim...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2012.00028

    authors: Pruitt RN,Lacy DB

    更新日期:2012-03-16 00:00:00

  • Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer.

    abstract::Esophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The present study charact...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00268

    authors: Li D,He R,Hou G,Ming W,Fan T,Chen L,Zhang L,Jiang W,Wang W,Lu Z,Feng H,Geng Q

    更新日期:2020-06-26 00:00:00

  • Astrocyte Apoptosis and HIV Replication Are Modulated in Host Cells Coinfected with Trypanosoma cruzi.

    abstract::The protozoan Trypanosoma cruzi is the etiological agent of Chagas disease. In immunosuppressed individuals, as it occurs in the coinfection with human immunodeficiency virus (HIV), the central nervous system may be affected. In this regard, reactivation of Chagas disease is severe and often lethal, and it accounts fo...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00345

    authors: Urquiza JM,Burgos JM,Ojeda DS,Pascuale CA,Leguizamón MS,Quarleri JF

    更新日期:2017-08-02 00:00:00

  • The inhibition of Platycodin D on Mycoplasma pneumoniae proliferation and its effect on promoting cell growth after anti-Mycoplasma pneumoniae treatment.

    abstract::Platycodin D, extract from the root of Platycodon grandiflorum, is one of the most important monomers of the Qinbaiqingfei pellets (Qinbai) that has already been approved as the first Traditional Chinese Medicine for clinic use as an anti-M. pneumoniae agent. Qinbai constituents Scutellaria baicalensis and Platycodon ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2014.00192

    authors: Meng Y,Yang Y,Lu W,Wang Y,Qian F,Wang X,Zhang Z,Wang W

    更新日期:2015-01-13 00:00:00

  • Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium.

    abstract::During intestinal invasion, Entamoeba histolytica opens tight junctions (TJs) reflected by transepithelial electrical resistance (TEER) dropping. To explore the molecular mechanisms underlying this, we studied in vitro and in vivo the damage produced by the recombinant E. histolytica cysteine protease (rEhCP112) on TJ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00372

    authors: Cuellar P,Hernández-Nava E,García-Rivera G,Chávez-Munguía B,Schnoor M,Betanzos A,Orozco E

    更新日期:2017-08-16 00:00:00

  • The Distinct Transcriptional Response of the Midgut of Amblyomma sculptum and Amblyomma aureolatum Ticks to Rickettsia rickettsii Correlates to Their Differences in Susceptibility to Infection.

    abstract::Rickettsia rickettsii is a tick-borne obligate intracellular bacterium that causes Rocky Mountain Spotted Fever (RMSF). In Brazil, two species of ticks in the genus Amblyomma, A. sculptum and A. aureolatum, are incriminated as vectors of this bacterium. Importantly, these two species present remarkable differences in ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00129

    authors: Martins LA,Galletti MFBM,Ribeiro JM,Fujita A,Costa FB,Labruna MB,Daffre S,Fogaça AC

    更新日期:2017-04-28 00:00:00

  • Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses.

    abstract::Chronic otitis media with effusion (COME) is a common childhood disease characterized by an accumulation of fluid behind the eardrum. COME often requires surgical intervention and can also lead to significant hearing loss and subsequent learning disabilities. Recent characterization of the middle ear fluid (MEF) micro...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00339

    authors: Kolbe AR,Castro-Nallar E,Preciado D,Pérez-Losada M

    更新日期:2019-10-04 00:00:00

  • Influence of the tryptophan-indole-IFNγ axis on human genital Chlamydia trachomatis infection: role of vaginal co-infections.

    abstract::The natural history of genital Chlamydia trachomatis infections can vary widely; infections can spontaneously resolve but can also last from months to years, potentially progressing to cause significant pathology. The host and bacterial factors underlying this wide variation are not completely understood, but emphasiz...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2014.00072

    authors: Aiyar A,Quayle AJ,Buckner LR,Sherchand SP,Chang TL,Zea AH,Martin DH,Belland RJ

    更新日期:2014-06-03 00:00:00

  • FcpB Is a Surface Filament Protein of the Endoflagellum Required for the Motility of the Spirochete Leptospira.

    abstract::The spirochete endoflagellum is a unique motility apparatus among bacteria. Despite its critical importance for pathogenesis, the full composition of the flagellum remains to be determined. We have recently reported that FcpA is a novel flagellar protein and a major component of the sheath of the filament of the spiro...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00130

    authors: Wunder EA Jr,Slamti L,Suwondo DN,Gibson KH,Shang Z,Sindelar CV,Trajtenberg F,Buschiazzo A,Ko AI,Picardeau M

    更新日期:2018-05-08 00:00:00

  • The influence of early neutrophil-Leishmania interactions on the host immune response to infection.

    abstract::Neutrophils are the first cells recruited to the dermal site of Leishmania infection following injection by needle or sand fly bite. The role of neutrophils in either promoting or suppressing host immunity remains controversial. We discuss the events driving neutrophil recruitment, their interaction with the parasite ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2012.00059

    authors: Ribeiro-Gomes FL,Sacks D

    更新日期:2012-05-04 00:00:00

  • Genotyping of Aspergillus fumigatus in Formalin-Fixed Paraffin-Embedded Tissues and Serum Samples From Patients With Invasive Aspergillosis.

    abstract::Invasive aspergillosis (IA) is a deep tissue infection with a high mortality occurring mostly in immunocompromised patients. To investigate the pathology of patients with IA it may be important to determine the genotype of the invasive isolate of Aspergillus, however available tissues for study are often formalin fixe...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00377

    authors: de Groot T,Hagen F,Vreuls W,Verweij PE,Chowdhary A,Meis JF

    更新日期:2018-10-23 00:00:00

  • Multiplex PCR Assays for the Detection of One Hundred and Thirty Seven Serogroups of Shiga Toxin-Producing Escherichia coli Associated With Cattle.

    abstract::Escherichia coli carrying prophage with genes that encode for Shiga toxins are categorized as Shiga toxin-producing E. coli (STEC) pathotype. Illnesses caused by STEC in humans, which are often foodborne, range from mild to bloody diarrhea with life-threatening complications of renal failure and hemolytic uremic syndr...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00378

    authors: Ludwig JB,Shi X,Shridhar PB,Roberts EL,DebRoy C,Phebus RK,Bai J,Nagaraja TG

    更新日期:2020-07-29 00:00:00

  • Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health.

    abstract::Streptococcus dentisani 7746, isolated from dental plaque of caries-free individuals, has been shown to have several beneficial effects in vitro which could contribute to promote oral health, including an antimicrobial activity against oral pathogens by the production of bacteriocins and a pH buffering capacity throug...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00465

    authors: Ferrer MD,López-López A,Nicolescu T,Perez-Vilaplana S,Boix-Amorós A,Dzidic M,Garcia S,Artacho A,Llena C,Mira A

    更新日期:2020-08-31 00:00:00

  • Evidencing the Role of Erythrocytic Apoptosis in Malarial Anemia.

    abstract::In the last decade it has become clear that, similarly to nucleated cells, enucleated red blood cells (RBCs) are susceptible to programmed apoptotic cell death. Erythrocytic apoptosis seems to play a role in physiological clearance of aged RBCs, but it may also be implicated in anemia of different etiological sources ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2016.00176

    authors: Totino PR,Daniel-Ribeiro CT,Ferreira-da-Cruz MF

    更新日期:2016-12-09 00:00:00

  • Dengue Non-structural Protein 5 Polymerase Complexes With Promyelocytic Leukemia Protein (PML) Isoforms III and IV to Disrupt PML-Nuclear Bodies in Infected Cells.

    abstract::Dengue virus (DENV) threatens almost 70% of the world's population, with no therapeutic currently available. The severe, potentially lethal forms of DENV disease (dengue hemorrhagic fever/dengue shock syndrome) are associated with the production of high level of cytokines, elicited as part of the host antiviral respon...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00284

    authors: Giovannoni F,Ladelfa MF,Monte M,Jans DA,Hemmerich P,García C

    更新日期:2019-08-13 00:00:00

  • Transition metal ions at the crossroads of mucosal immunity and microbial pathogenesis.

    abstract::Transition metal ions are essential micronutrients for all living organisms. In mammals, these ions are often protein-bound and sequestered within cells, limiting their availability to microbes. Moreover, in response to infection, mammalian hosts further reduce the availability of metal nutrients by activating epithel...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2014.00002

    authors: Diaz-Ochoa VE,Jellbauer S,Klaus S,Raffatellu M

    更新日期:2014-01-24 00:00:00

  • Epidemiology of Pneumocystis jirovecii Pneumonia and (Non-)use of Prophylaxis.

    abstract::Objectives:Pneumocystis jirovecii pneumonia (PCP) is an AIDS-defining illness. In patients with HIV, the benefit of PCP prophylaxis is well-defined when the CD4 T-cell count decreases below 200 cells/μL. In other immunocompromised patients, the value of PCP prophylaxis is not always as well-established. This study aim...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00224

    authors: Dunbar A,Schauwvlieghe A,Algoe S,van Hellemond JJ,Reynders M,Vandecasteele S,Boelens J,Depuydt P,Rijnders B

    更新日期:2020-05-15 00:00:00

  • Exosome-Transmitted miR-25 Induced by H. pylori Promotes Vascular Endothelial Cell Injury by Targeting KLF2.

    abstract::Background: Increasing evidence has shown that Helicobacter pylori is associated with coronary heart disease (CHD); however, the underlying mechanism remains unclear. Methods: The expression of miR-25 and mRNAs was measured using qRT-PCR. Protein levels were detected using western blotting and exosomes were assessed w...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00366

    authors: Li N,Liu SF,Dong K,Zhang GC,Huang J,Wang ZH,Wang TJ

    更新日期:2019-10-29 00:00:00

  • Identification of the PANoptosome: A Molecular Platform Triggering Pyroptosis, Apoptosis, and Necroptosis (PANoptosis).

    abstract::Programmed cell death plays crucial roles in organismal development and host defense. Recent studies have highlighted mechanistic overlaps and extensive, multifaceted crosstalk between pyroptosis, apoptosis, and necroptosis, three programmed cell death pathways traditionally considered autonomous. The growing body of ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00237

    authors: Christgen S,Zheng M,Kesavardhana S,Karki R,Malireddi RKS,Banoth B,Place DE,Briard B,Sharma BR,Tuladhar S,Samir P,Burton A,Kanneganti TD

    更新日期:2020-05-29 00:00:00

  • Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans.

    abstract::Iron is the most abundant transition metal in the human body and its bioavailability is stringently controlled. In particular, iron is tightly bound to host proteins such as transferrin to maintain homeostasis, to limit potential damage caused by iron toxicity under physiological conditions and to restrict access by p...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2013.00080

    authors: Caza M,Kronstad JW

    更新日期:2013-11-19 00:00:00

  • Association Analysis on Recurrence of Bacterial Vaginosis Revealed Microbes and Clinical Variables Important for Treatment Outcome.

    abstract::To investigate the parameters associated with post-treatment recurrence of bacterial vaginosis (BV), clinical factors and vaginal microbiota were examined and analyzed for BV patients who received standard metronidazole therapy. The variables associated with BV recurrence included clinical factors of past BV history, ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00189

    authors: Xiao B,Wu C,Song W,Niu X,Qin N,Liu Z,Xu Q

    更新日期:2019-06-11 00:00:00

  • CFTR Depletion Confers Hypersusceptibility to Mycobacterium fortuitum in a Zebrafish Model.

    abstract::The Mycobacterium fortuitum complex comprises several closely related species, causing pulmonary and extra-pulmonary infections. However, there is very limited knowledge about the disease pathogenesis involved in M. fortuitum infections, particularly due to the lack of suitable animal models. Using the zebrafish model...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00357

    authors: Johansen MD,Kremer L

    更新日期:2020-07-17 00:00:00

  • An Updated View on the Rck Invasin of Salmonella: Still Much to Discover.

    abstract::Salmonella is a facultative intracellular Gram-negative bacterium, responsible for a wide range of food- and water-borne diseases ranging from gastroenteritis to typhoid fever depending on hosts and serotypes. Salmonella thus represents a major threat to public health. A key step in Salmonella pathogenesis is the inva...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2017.00500

    authors: Mambu J,Virlogeux-Payant I,Holbert S,Grépinet O,Velge P,Wiedemann A

    更新日期:2017-12-08 00:00:00

  • Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis.

    abstract::Chlamydiae are obligate intracellular pathogens of eukaryotes. The bacteria grow in an intracellular vesicle called an inclusion, the membrane of which is heavily modified by chlamydial proteins called Incs (Inclusion membrane proteins). Incs represent 7-10% of the genomes of Chlamydia and, given their localization at...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2015.00013

    authors: Gauliard E,Ouellette SP,Rueden KJ,Ladant D

    更新日期:2015-02-11 00:00:00

  • Cellular Markers of Active Disease and Cure in Different Forms of Leishmania infantum-Induced Disease.

    abstract::Increased numbers of peripheral blood mononucleocytes (PBMC) and increased IFN-γ secretion following in vitro challenge of blood samples with soluble Leishmania antigen (SLA), have been proposed as biomarkers of specific cell-mediated immunity, indicating that treatment of visceral leishmaniasis (VL) has been successf...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00381

    authors: Botana L,Matía B,San Martin JV,Romero-Maté A,Castro A,Molina L,Fernandez L,Ibarra-Meneses A,Aguado M,Sánchez C,Horrillo L,Chicharro C,Nieto J,Ortega S,Ruiz-Giardin JM,Carrillo E,Moreno J

    更新日期:2018-11-13 00:00:00

  • A Metagenome-Wide Association Study of Gut Microbiome in Patients With Multiple Sclerosis Revealed Novel Disease Pathology.

    abstract::While microbiome plays key roles in the etiology of multiple sclerosis (MS), its mechanism remains elusive. Here, we conducted a comprehensive metagenome-wide association study (MWAS) of the relapsing-remitting MS gut microbiome (ncase = 26, ncontrol = 77) in the Japanese population, by using whole-genome shotgun sequ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.585973

    authors: Kishikawa T,Ogawa K,Motooka D,Hosokawa A,Kinoshita M,Suzuki K,Yamamoto K,Masuda T,Matsumoto Y,Nii T,Maeda Y,Nakamura S,Inohara H,Mochizuki H,Okuno T,Okada Y

    更新日期:2020-12-11 00:00:00

  • The Combination Vaccine Adjuvant System Alum/c-di-AMP Results in Quantitative and Qualitative Enhanced Immune Responses Post Immunization.

    abstract::The development of new effective vaccines strongly depends on adjuvants and formulations able to stimulate not only strong humoral responses against a certain pathogen but also effector as well as memory CD4+ and CD8+ T cells (Dubensky et al., 2013). However, the majority of vaccines licensed for human use or currentl...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00031

    authors: Ebensen T,Delandre S,Prochnow B,Guzmán CA,Schulze K

    更新日期:2019-02-19 00:00:00

  • Vaccine delivery using nanoparticles.

    abstract::Vaccination has had a major impact on the control of infectious diseases. However, there are still many infectious diseases for which the development of an effective vaccine has been elusive. In many cases the failure to devise vaccines is a consequence of the inability of vaccine candidates to evoke appropriate immun...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2013.00013

    authors: Gregory AE,Titball R,Williamson D

    更新日期:2013-03-25 00:00:00

  • The Transcriptome of Exophiala dermatitidis during Ex-vivo Skin Model Infection.

    abstract::The black yeast Exophiala dermatitidis is a widespread polyextremophile and human pathogen, that is found in extreme natural habitats and man-made environments such as dishwashers. It can cause various diseases ranging from phaeohyphomycosis and systemic infections, with fatality rates reaching 40%. While the number o...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2016.00136

    authors: Poyntner C,Blasi B,Arcalis E,Mirastschijski U,Sterflinger K,Tafer H

    更新日期:2016-10-24 00:00:00