Benzodiazepine receptor and thyroid hormones: in vivo and in vitro modulation.

abstract：:The RNA integrity number (RIN) is often considered to be a critical measure of the quality of postmortem human brains. However, it has been suggested that RINs do not necessarily reflect the availability of intact mRNA. Using the Agilent bioanalyzer and qRT-PCR, we explored whether RINs provide a meaningful way of ass...

journal_title：Journal of neurochemistry

authors： Sonntag KC,Tejada G,Subburaju S,Berretta S,Benes FM,Woo TU

更新日期：2016-07-01 00:00:00

abstract：:Three conserved serine residues (Ser193, Ser194, and Ser197) in transmembrane spanning region (TM) V of the D2 dopamine receptor have been mutated to alanine, individually and in combination, to explore their role in ligand binding and G protein coupling. The multiple Ser -->Ala mutations had no effect on the binding ...

journal_title：Journal of neurochemistry

authors： Coley C,Woodward R,Johansson AM,Strange PG,Naylor LH

更新日期：2000-01-01 00:00:00

abstract：:The interaction of triethyltin (TET) and trimethyltin (TMT) with rat brain astrocytes in vitro was investigated. Both compounds are highly neurotoxic after in vivo application, cause neurobehavioral changes, and elicit neuronal and glial responses in the CNS. In this study, 5-week-old cultures were exposed to TMT or T...

journal_title：Journal of neurochemistry

authors： Richter-Landsberg C,Besser A

更新日期：1994-12-01 00:00:00

abstract：:Capsaicin receptors are expressed in primary sensory neurons and excited by heat and protons. We examined the inflammation-induced changes of the level of VR1 capsaicin receptor mRNA in sensory neurons and the sensitivity of primary afferents to capsaicin. Carrageenan treatment induced axonal transport of VR1 mRNA, bu...

journal_title：Journal of neurochemistry

authors： Tohda C,Sasaki M,Konemura T,Sasamura T,Itoh M,Kuraishi Y

更新日期：2001-03-01 00:00:00

abstract：:Within the cell membrane there exist various microdomains (lipid rafts) in which specific lipids and proteins are assembled and these microdomains are recovered in the detergent-resistant low-density membrane fraction (DRM). Septin is a novel GTP-binding, cytoskeletal protein having various isoforms that assemble into...

journal_title：Journal of neurochemistry

authors： Maimaitiyiming M,Kumanogoh H,Nakamura S,Nagata K,Suzaki T,Maekawa S

更新日期：2008-08-01 00:00:00

abstract：:The protein kinase C γ (PKCγ) undergoes multistep activation and participates in various cellular processes in Purkinje cells. Perturbations in its phosphorylation state, conformation or localization can disrupt kinase signalling, such as in spinocerebellar ataxia type 14 (SCA14) that is caused by missense mutations i...

journal_title：Journal of neurochemistry

authors： Jezierska J,Goedhart J,Kampinga HH,Reits EA,Verbeek DS

更新日期：2014-03-01 00:00:00

abstract：:Action mechanism of lipopolysaccharide (LPS), interleukin-1β (IL-1β), and lysophosphatidic acid (LPA) to regulate motility, an important process of astrogliosis, was investigated in rat astrocytes. While LPA exerted no significant effect on the cell migration, the prior treatment of the cells with LPS or IL-1β resulte...

journal_title：Journal of neurochemistry

authors： Sato K,Horiuchi Y,Jin Y,Malchinkhuu E,Komachi M,Kondo T,Okajima F

更新日期：2011-04-01 00:00:00

abstract：:To provide insights into the effects of temporary focal ischemia on the function of neurons and astrocytes in vivo, we measured the incorporation of radiolabel from [U-14C]glucose into both glutamate and glutamine in brain subregions at 1 h of reperfusion following occlusion of the middle cerebral artery for 2 or 3 h....

journal_title：Journal of neurochemistry

authors： Thoren AE,Helps SC,Nilsson M,Sims NR

更新日期：2006-05-01 00:00:00

abstract：:Excessive N-methyl-D-aspartate (NMDA) signaling is thought to contribute to bipolar disorder symptoms. Lithium and carbamazepine, effective against bipolar mania, are reported in rats to reduce brain transcription of an arachidonic acid selective calcium-dependent cytosolic phospholipase A(2) (cPLA(2)), as well as exp...

journal_title：Journal of neurochemistry

authors： Rao JS,Ertley RN,Rapoport SI,Bazinet RP,Lee HJ

更新日期：2007-09-01 00:00:00

abstract：:The olfactory bulb (OB) contains the highest concentration of the insulin receptor (IR) kinase in the central nervous system; however, its functional role and modulation in this region remains poorly understood. IR kinase contains a number of proline-rich motifs, making it an excellent candidate for modulation by SH(3...

journal_title：Journal of neurochemistry

authors： Marks DR,Fadool DA

更新日期：2007-11-01 00:00:00

abstract：:Adrenomedullin, originally discovered from pheochromocytoma, is a member of the calcitonin gene-related peptide family. The production and secretion of adrenomedullin by cultured human astrocytes were studied by northern blot analysis and radioimmunoassay. Northern blot analysis showed the expression of adrenomedullin...

journal_title：Journal of neurochemistry

authors： Takahashi K,Nakayama M,Totsune K,Murakami O,Sone M,Kitamuro T,Yoshinoya A,Shibahara S

更新日期：2000-01-01 00:00:00

abstract：:Recent evidence supports the emerging hypothesis that the amyloid-β precursor protein C-terminal fragments (APP-CTFs) and dysregulations in both their qualitative and quantitative productions may actively and directly contribute to the neuronal toxicity in early phases of Alzheimer's disease (AD). These new findings r...

journal_title：Journal of neurochemistry

authors： Mosser S,Gerber H,Fraering PC

更新日期：2020-08-05 00:00:00

abstract：:The rat adrenal hormone corticosterone binds to low and high affinity receptors, discretely localized in brain, including the dentate gyrus. Differential activation of the two receptor types under physiological conditions alters gene expression and functional characteristics of hippocampal neurones. In the present stu...

journal_title：Journal of neurochemistry

authors： Qin Y,Karst H,Joëls M

更新日期：2004-04-01 00:00:00

abstract：:Rat brain succinyl-CoA:3-oxo-acid CoA-transferase (3-oxo-acid CoA-transferase, EC 2.8.3.5), the first committed enzyme in the oxidation of ketone bodies in mitochondria, was purified to apparent homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme has an apparent molecular weight of 90,000 as determ...

journal_title：Journal of neurochemistry

authors： Russell JJ,Patel MS

更新日期：1982-05-01 00:00:00

abstract：:The antisense strategy was used to unravel the functional contribution of the mRNAs encoding dopamine (DA) receptors to the multiple transduction mechanisms operated by DA in rat pituitary cells. An antisense oligonucleotide was designed to recognize seven nucleotides upstream and 11 nucleotides downstream from the in...

journal_title：Journal of neurochemistry

authors： Valerio A,Alberici A,Tinti C,Spano P,Memo M

更新日期：1994-04-01 00:00:00

abstract：:PRMT1, a major arginine methyltransferase, plays critical roles in transcription, DNA damage response, and cell proliferation. Although we have previously discovered the crucial roles of PRMT1 for oligodendrocyte lineage progression in the central nervous system of neural stem cell-specific PRMT1 conditional knockout ...

journal_title：Journal of neurochemistry

authors： Hashimoto M,Kumabe A,Kim JD,Murata K,Sekizar S,Williams A,Lu W,Ishida J,Nakagawa T,Endo M,Minami Y,Fukamizu A

更新日期：2020-08-16 00:00:00

abstract：:Vesicular sequestration is important in the regulation of cytoplasmic concentrations of monoamines such as dopamine. Moreover, recent evidence suggests that increases in cytoplasmic dopamine levels, perhaps attributable to changes in vesicular monoamine transporter function, contribute to methamphetamine-induced dopam...

journal_title：Journal of neurochemistry

authors： Brown JM,Hanson GR,Fleckenstein AE

更新日期：2000-05-01 00:00:00

abstract：:High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. o...

journal_title：Journal of neurochemistry

authors： Puinean AM,Lansdell SJ,Collins T,Bielza P,Millar NS

更新日期：2013-03-01 00:00:00

abstract：:In this study, we show specific intracellular responses evoked by the stimulation of astrocytes with the P1,P5-di(adenosine-5')pentaphosphate, Ap5A. The stimulation of astrocytes with micromolar concentrations of the dinucleotide elicited rapid increases in intracellular calcium concentration ([Ca2+]i), showing an EC5...

journal_title：Journal of neurochemistry

authors： Jiménez AI,Castro E,Delicado EG,Miras-Portugal MT

更新日期：2002-10-01 00:00:00

abstract：:The kinetics of the reactions of nine opioid peptides with the neutral endopeptidase ("enkephalinase") activities of human kidney, rat kidney, and rat brain have been determined. These opioid peptides can be divided into two classes, those that are good inhibitors of Leu5-enkephalin hydrolysis (Ki less than 75 microM)...

journal_title：Journal of neurochemistry

authors： Hersh LB

更新日期：1984-08-01 00:00:00

abstract：:Brain gangliosides from four vertebrate classes (fish, amphibia, aves, and mammalia) were studied by patterns generated with the use of two-dimensional thin-layer chromatography. In an effort to retain possible alkalilabile gangliosides, samples were not base-hydrolyzed. Resultant chromatograms revealed complex patter...

journal_title：Journal of neurochemistry

authors： Hunter GD,Wiegant VM,Dunn AJ

更新日期：1981-10-01 00:00:00

abstract：:The aim of the present study was to explore the possible role of kappa/dynorphin system in the development of tolerance to nicotine antinociception in mice. First, we observed that kappa-opioid receptor (KOP-r) participates in the acute spinal antinociception produced by nicotine (3 and 5 mg/kg, s.c.) since the pre-tr...

journal_title：Journal of neurochemistry

authors： Galeote L,Maldonado R,Berrendero F

更新日期：2008-05-01 00:00:00

abstract：:We investigated the role of Ca(2+)-dependent protein kinases in the regulation of astrocytic cell volume. Calmodulin (CaM) antagonists were used to inhibit CaM and thus Ca2+/CaM-dependent protein kinase. The effect of these inhibitors as well as activators and inhibitors of protein kinase C (PKC) on astrocytic volume ...

journal_title：Journal of neurochemistry

authors： Bender AS,Neary JT,Blicharska J,Norenberg LO,Norenberg MD

更新日期：1992-05-01 00:00:00

abstract：:Recent evidence indicates that supplementation with a specific combination of nutrients may affect cell membrane synthesis and composition. To investigate whether such nutrients may also modify the physical properties of membranes, and affect membrane-bound processes involved in signal transduction pathways, we studie...

journal_title：Journal of neurochemistry

authors： Savelkoul PJ,Janickova H,Kuipers AA,Hageman RJ,Kamphuis PJ,Dolezal V,Broersen LM

更新日期：2012-02-01 00:00:00

abstract：:Intracellular protein inclusions in Alzheimer's disease and progressive supranuclear palsy contain UBB+1, a variant ubiquitin. UBB+1 is able block the 26S proteasome in cell lines. Proteasome inhibition by drug action has previously been shown to induce a heat-shock response and render protection against stress. We in...

journal_title：Journal of neurochemistry

authors： Hope AD,de Silva R,Fischer DF,Hol EM,van Leeuwen FW,Lees AJ

更新日期：2003-07-01 00:00:00

abstract：:We used both the polymerase chain reaction (PCR) and in situ hybridization to search for the presence of proteolipid protein (PLP) gene transcripts in the developing mouse. Total brain RNA extracted from 13-19-day embryos, analyzed by PCR, demonstrated the presence of a single transcript that was unambiguously identif...

journal_title：Journal of neurochemistry

authors： Timsit SG,Bally-Cuif L,Colman DR,Zalc B

更新日期：1992-03-01 00:00:00

abstract：:Different forms of acetylcholinesterase (AChE), EC 3.1.1.7, were demonstrated in human brain caudate nucleus. One form was solubilized at high ionic strength, the other with Triton X-100. The detergent-extractable form was purified to homogeneity by affinity chromatography. This form of AChE is amphiphile-dependent; i...

journal_title：Journal of neurochemistry

authors： Sørensen K,Gentinetta R,Brodbeck U

更新日期：1982-10-01 00:00:00

abstract：:Purkinje cell degeneration (pcd) was first identified in a spontaneous mouse mutant showing cerebellar ataxia. In addition to cerebellar Purkinje cells (PCs), retinal photoreceptors, mitral cells in the olfactory bulb, and a discrete subpopulation of thalamic neurons also degenerate in the mutant brains. The gene resp...

journal_title：Journal of neurochemistry

authors： Zhou L,Hossain MI,Yamazaki M,Abe M,Natsume R,Konno K,Kageyama S,Komatsu M,Watanabe M,Sakimura K,Takebayashi H

更新日期：2018-11-01 00:00:00

abstract：:Administration of L-DOPA (50 mg/kg) elicits a significant increase in extracellular dopamine in striata of rats treated with the catecholaminergic neurotoxin 6-hydroxydopamine but not in striata of intact rats. To assess the role of dopaminergic nerve terminals in determining the effects of exogenous L-DOPA on extrace...

journal_title：Journal of neurochemistry

authors： Wachtel SR,Abercrombie ED

更新日期：1994-07-01 00:00:00

abstract：:Intraperitoneal injection of the cyclic imine 1-piperideine in mice resulted in measurable quantities of 5-aminopentanoic acid in brain. 5-Aminopentanoic acid is a methylene homologue of gamma-aminobutyric acid (GABA) that is a weak GABA agonist. 5-Aminopentanoic acid formed in the periphery was ruled out as the sourc...

journal_title：Journal of neurochemistry

authors： Callery PS,Geelhaar LA

更新日期：1985-09-01 00:00:00