Specific diadenosine pentaphosphate receptor coupled to extracellular regulated kinases in cerebellar astrocytes.

Abstract:

:In this study, we show specific intracellular responses evoked by the stimulation of astrocytes with the P1,P5-di(adenosine-5')pentaphosphate, Ap5A. The stimulation of astrocytes with micromolar concentrations of the dinucleotide elicited rapid increases in intracellular calcium concentration ([Ca2+]i), showing an EC50 value of 15.27 +/- 0.61 micro m. Moreover, the stimulation of cells with nanomolar concentrations of Ap5A, unable to induce calcium responses, increased the phosphorylated forms of extracellular-signal regulated kinase 1/2 (ERK) with an EC50 value of 9.8 +/- 2.4 nm. The maximal activation was observed at 100 nm Ap5A, which was similar to that produced by epidermal growth factor (EGF) under the same experimental conditions. The present data reported here indicate that Ap5A mediated these effects by interacting with a specific receptor, not yet identified, which was different from the P2Y1 and P2Y2/P2Y4 receptors present in all individual astrocytes.

journal_name

J Neurochem

authors

Jiménez AI,Castro E,Delicado EG,Miras-Portugal MT

doi

10.1046/j.1471-4159.2002.01111.x

keywords:

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

299-308

issue

2

eissn

0022-3042

issn

1471-4159

pii

1111

journal_volume

83

pub_type

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