The picrotoxinin binding site and its relationship to the GABA receptor complex.

abstract：:Studies suggest that activation of phosphoinositide 3-kinase-Akt may protect against neuronal cell death in Alzheimer's disease (AD). Here, however, we provide evidence of increased Akt activation, and hyperphosphorylation of critical Akt substrates in AD brain, which link to AD pathogenesis, suggesting that treatment...

journal_title：Journal of neurochemistry

authors： Griffin RJ,Moloney A,Kelliher M,Johnston JA,Ravid R,Dockery P,O'Connor R,O'Neill C

更新日期：2005-04-01 00:00:00

abstract：:The importance of the nuclear receptor nurr1 for the appropriate development of mesencephalic dopamine-synthesizing neurons has been clearly demonstrated through the targeted disruption of the nurr1 gene. The persistence of nurr1 expression in adult tissue suggests a possible role for this transcription factor in the ...

journal_title：Journal of neurochemistry

authors： Sacchetti P,Mitchell TR,Granneman JG,Bannon MJ

更新日期：2001-03-01 00:00:00

abstract：:Increased awareness for a role of oxidative stress in the pathogenesis of Alzheimer's disease has highlighted the issue of whether oxidative damage is a fundamental step in the pathogenesis or instead results from disease-associated pathology. In vitro experiments support both possibilities: Oxidative stress increases...

journal_title：Journal of neurochemistry

authors： Smith MA,Hirai K,Hsiao K,Pappolla MA,Harris PL,Siedlak SL,Tabaton M,Perry G

更新日期：1998-05-01 00:00:00

abstract：:2,4,5-Trihydroxyphenylalanine (6-OH-DOPA) destroys central and peripheral noradrenergic neurons, while sparing dopaminergic neurons. Previous studies indicate that 6-OH-DOPA toxicity is mediated by the formation of 6-hydroxydopamine. However, levels of 6-hydroxydopamine in brain following peripheral administration of ...

journal_title：Journal of neurochemistry

authors： Evans JM,Cohen G

更新日期：1989-05-01 00:00:00

abstract：:The aim of this study was to examine the effects of the phorbol ester O-tetradecanoylphorbol 13-acetate (TPA) and forskolin on delta-opioid receptor gene transcription. Treatment of NG108-15 cells with TPA (100 nM) for 48 h increased delta-opioid receptor mRNA levels, whereas different concentrations of forskolin indu...

journal_title：Journal of neurochemistry

authors： Wöltje M,Kraus J,Höllt V

更新日期：2000-04-01 00:00:00

abstract：:CNS myelin was isolated from the spinal cord of the African lungfish Protopterus dolloi. Its proteins consisted of (1) two basic proteins (16,000 and 18,500 apparent Mr) that reacted with anti-human CNS myelin basic protein antibodies and (2) a major protein (29,000 apparent Mr) that stained with concanavalin A-horser...

journal_title：Journal of neurochemistry

authors： Waehneldt TV,Matthieu JM,Jeserich G

更新日期：1986-05-01 00:00:00

abstract：:Nerve growth factor (NGF) serves a critical survival-promoting function for developing sympathetic neurons. Following removal of NGF, sympathetic neurons undergo apoptosis characterized by the activation of c-Jun N-terminal kinases (JNKs), up-regulation of BH3-only proteins including BcL-2-interacting mediator of cell...

journal_title：Journal of neurochemistry

authors： Lomb DJ,Straub JA,Freeman RS

更新日期：2007-12-01 00:00:00

abstract：:Voltage-dependent calcium channels from ileal smooth muscle can be affinity-labeled with a [3H]dihydropyridine isothiocyanate radioligand. We examined the binding of this agent to brain membranes, to compare the properties of calcium channel drug binding sites in brain with those previously described in ileum. In brai...

journal_title：Journal of neurochemistry

authors： Greenberg DA,Cooper EC,Carpenter CL

更新日期：1985-01-01 00:00:00

abstract：:Lactoferrin (Lf) is an iron-binding protein involved in host defense against infection and severe inflammation, which accumulates in the brain during neurodegenerative disorders. Prior to determining Lf function in pathological brain tissues, we investigated its transport through the blood-brain barrier (BBB) in infla...

journal_title：Journal of neurochemistry

authors： Fillebeen C,Dehouck B,Benaïssa M,Dhennin-Duthille I,Cecchelli R,Pierce A

更新日期：1999-12-01 00:00:00

abstract：:The present study demonstrates that [3H]imipramine binds to both high- and low-affinity imipramine binding components on membranes prepared from rat cerebral cortex. Scatchard and computer analyses of saturation experiments using a wide range of [3H]imipramine concentrations (0.5 nM-50 nM) revealed the presence of two...

journal_title：Journal of neurochemistry

authors： Conway PG,Brunswick DJ

更新日期：1985-07-01 00:00:00

abstract：:The brain adapts to dynamic environmental conditions by altering its epigenetic state, thereby influencing neuronal transcriptional programs. An example of an epigenetic modification is protein methylation, catalyzed by protein arginine methyltransferases (PRMT). One member, Prmt8, is selectively expressed in the cent...

journal_title：Journal of neurochemistry

authors： Lee PK,Goh WW,Sng JC

更新日期：2017-02-01 00:00:00

abstract：:Specific D2 binding in rat striatum was characterized and then the effects of chronic disruption of dopaminergic activity on antagonist and agonist binding to these sites were studied. D2 receptors were defined as those sites capable of binding [3H]spiperone in the presence of cinanserin, a 5-HT2 antagonist, but not i...

journal_title：Journal of neurochemistry

authors： MacKenzie RG,Zigmond MJ

更新日期：1984-11-01 00:00:00

abstract：:β-Site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) is the transmembrane aspartyl protease that catalyzes the first cleavage step in the proteolysis of the APP to the amyloid β-protein (Aβ), a process involved in the pathogenesis of Alzheimer disease. BACE1 pre-mRNA undergoes complex alternative splicing,...

journal_title：Journal of neurochemistry

authors： Fisette JF,Montagna DR,Mihailescu MR,Wolfe MS

更新日期：2012-06-01 00:00:00

abstract：:The rat adrenal hormone corticosterone binds to low and high affinity receptors, discretely localized in brain, including the dentate gyrus. Differential activation of the two receptor types under physiological conditions alters gene expression and functional characteristics of hippocampal neurones. In the present stu...

journal_title：Journal of neurochemistry

authors： Qin Y,Karst H,Joëls M

更新日期：2004-04-01 00:00:00

abstract：:The suprachiasmatic nucleus houses the central circadian clock and is characterized by the timely regulated expression of clock genes. However, neurons of the cerebellar cortex also contain a circadian oscillator with circadian expression of clock genes being controlled by the suprachiasmatic nucleus. It has been sugg...

journal_title：Journal of neurochemistry

authors： Bering T,Carstensen MB,Rath MF

更新日期：2017-09-01 00:00:00

abstract：:We examined the potential importance of dexamethasone-mediated alterations in energy metabolism in providing protection against hypoxic-ischemic brain damage in immature rats. Seven-day-old rats (n = 165) that had been treated with dexamethasone (0.1 mg/kg, i.p.) or vehicle were assigned to control or hypoxic-ischemic...

journal_title：Journal of neurochemistry

authors： Tuor UI,Yager JY,Bascaramurty S,Del Bigio MR

更新日期：1997-11-01 00:00:00

abstract：:Following a previous report on detection of muscarinic receptors in myelin with the implied presence of G proteins, we now demonstrate by more direct means the presence of such proteins and their quantification. Using [35S]guanosine 5'-O-(3-thiotriphosphate) ([35S]GTP gamma S) as the binding ligand, purified myelin fr...

journal_title：Journal of neurochemistry

authors： Larocca JN,Golly F,Ledeen RW

更新日期：1991-07-01 00:00:00

abstract：:The kappa-opioid receptor agonists including U-50,488H and dynorphin A (1-17) in ranges of 0.1-100 nM inhibited the hydrolysis of GTP to GDP (P(i) release) inherent in GTP-binding proteins (G proteins) in guinea pig cerebellar membranes. U-50,488H inhibited only high-affinity GTPase activity, not low-affinity activity...

journal_title：Journal of neurochemistry

authors： Ueda H,Misawa H,Fukushima N,Katada T,Ui M,Satoh M

更新日期：1996-02-01 00:00:00

abstract：:Variations occurring in cortical nitric oxide (NO) release were analysed with a voltametric method in rats (i) placed in control conditions, (ii) while being paradoxical sleep deprived (PSD), or (iii) recovering from a PSD. Activities of neuronal (nNOS) and inducible (iNOS) NO-synthases as well as nNOS expression were...

journal_title：Journal of neurochemistry

authors： Clément P,Sarda N,Cespuglio R,Gharib A

更新日期：2004-08-01 00:00:00

abstract：:Ganglioside GM1, tritiated at the level of the long chain base (sphingosine) [( Sph-3H]GM1), sialic acid (N-acetylneuraminic acid) [( NeuAc-3H]GM1), or terminal galactose [( Gal-3H]GM1) was supplied to cerebellar granule cells differentiated in vitro, and its metabolic processing was followed with pulse time. Using [S...

journal_title：Journal of neurochemistry

authors： Riboni L,Tettamanti G

更新日期：1991-12-01 00:00:00

abstract：:Two biochemical strategies using nick translation-type of incubation and terminal tranferase-catalyzed reaction were used to assess single-(SSB) and double-strand (DSB) breaks in DNA of permeabilized neurons isolated from young, adult, and old rat cerebral cortex. Both SSBs and DSBs accumulate with age. On prior treat...

journal_title：Journal of neurochemistry

authors： Mandavilli BS,Rao KS

更新日期：1996-10-01 00:00:00

abstract：:The uptake of glutamate and other acidic amino acids into barnacle single muscle fibres has been characterized. The uptake of glutamate consists of two components, one Na-independent and one Na-dependent. The Na-dependent uptake is saturable (half-maximal at 250 microM external glutamate) and is inhibited by a variety...

journal_title：Journal of neurochemistry

authors： Revest PA,Baker PF

更新日期：1988-01-01 00:00:00

abstract：:The behavior of a microdialysis probe in vivo is mathematically described. A diffusion-reaction model is developed that not only accounts for transport of substances through tissues and probe membranes but also accounts for transport across the microvasculature and metabolism. Time-dependent equations are presented bo...

journal_title：Journal of neurochemistry

authors： Morrison PF,Bungay PM,Hsiao JK,Ball BA,Mefford IN,Dedrick RL

更新日期：1991-07-01 00:00:00

abstract：:Mutations in the presenilin 1 (PS1) gene are associated with autosomal dominant, early-onset, familial Alzheimer's disease and result in increased release of the hyperaggregatable 42-amino acid form of the amyloid beta-peptide (A(beta)42). To determine which subcellular compartments are potential source(s) of released...

journal_title：Journal of neurochemistry

authors： Petanceska SS,Seeger M,Checler F,Gandy S

更新日期：2000-05-01 00:00:00

abstract：:The electrogenic tissue of Torpedo was found to phosphorylate in vitro external [14C]thiamine by a saturable process. The rate of this metabolisaton was increased when acetate, an efficient precursor of acetylcholine (ACh) synthesis in this tissue, was added to the incubation medium, thus increasing the turnover of AC...

journal_title：Journal of neurochemistry

authors： Eder L,Dunant Y,Loctin F

更新日期：1980-12-01 00:00:00

abstract：:A protein has been purified from human brain that appears to be the human equivalent of bovine 14-3-3 protein. On polyacrylamide gel electrophoresis the protein migrates as a faster major component, termed 14-3-3-2 protein, and a slower minor component, termed 14-3-3-1 protein, which consists of approximately 12% of t...

journal_title：Journal of neurochemistry

authors： Boston PF,Jackson P,Kynoch PA,Thompson RJ

更新日期：1982-05-01 00:00:00

abstract：:In complex tissues where multiple subtypes of nicotinic acetylcholine receptors (nAChRs) are expressed, immunohistochemistry has been the most popular tool for investigation of nAChR subunit distribution. However, recent studies with nAChR subunit knockout mice demonstrated that a large panel of antibodies is unsuitab...

journal_title：Journal of neurochemistry

authors： Shelukhina IV,Kryukova EV,Lips KS,Tsetlin VI,Kummer W

更新日期：2009-05-01 00:00:00

abstract：:The irreversible inhibition of the monoamine oxidase (MAO) activity within monoaminergic neurons in the rat brain 24 h after single or repeated administration of (E)-beta-fluoromethylene-m-tyrosine (FMMT, MDL 72394) was examined. The enzyme activity was determined by incubating synaptosome-rich homogenates of hypothal...

journal_title：Journal of neurochemistry

authors： Fagervall I,Ross SB

更新日期：1989-02-01 00:00:00

abstract：:We have correlated myelin membrane structure with biochemical composition in the CNS and PNS of a phylogenetic series of animals, including elasmobranchs, teleosts, amphibians, and mammals. X-ray diffraction patterns were recorded from freshly dissected, unfixed tissue and used to determine the thicknesses of the liqu...

journal_title：Journal of neurochemistry

authors： Kirschner DA,Inouye H,Ganser AL,Mann V

更新日期：1989-11-01 00:00:00

abstract：:Chronic exposure to manganese (Mn) can cause manganism, a neurodegenerative disorder similar to Parkinson's disease. The toxicity of Mn includes impairment of astrocytic glutamate transporters. 17beta-Estradiol (E2) has been shown to be neuroprotective in various neurodegenerative diseases including Parkinson's diseas...

journal_title：Journal of neurochemistry

authors： Lee ES,Sidoryk M,Jiang H,Yin Z,Aschner M

更新日期：2009-07-01 00:00:00