A subtype of kappa-opioid receptor mediates inhibition of high-affinity GTPase inherent in Gi1 in guinea pig cerebellar membranes.


:The kappa-opioid receptor agonists including U-50,488H and dynorphin A (1-17) in ranges of 0.1-100 nM inhibited the hydrolysis of GTP to GDP (P(i) release) inherent in GTP-binding proteins (G proteins) in guinea pig cerebellar membranes. U-50,488H inhibited only high-affinity GTPase activity, not low-affinity activity. The action of this agonist was found to be biphasic, and there was no inhibition at concentrations > 1 microM. The inhibition was abolished by pretreatment with preactivated pertussis toxin (PTX) at concentrations > 1 micrograms/ml but not with preactivated cholera toxin (30 micrograms/ml). Similar blockade of kappa-receptor-mediated inhibition was also observed when membranes were pretreated with a low concentration (8 microM) of N-ethylmaleimide (NEM) at low temperature (4 degrees C), which alkylates the cysteine residue to be ADP-ribosylated by PTX; but this treatment caused no significant change in kappa-agonist binding. When purified Gi1, but not G(o), was reconstituted into membranes pretreated with NEM, the kappa-receptor-mediated inhibition was recovered. These findings suggest that a subtype of kappa-opioid receptor is coupled to inhibition of intrinsic activity of Gi1.


J Neurochem


Ueda H,Misawa H,Fukushima N,Katada T,Ui M,Satoh M




Has Abstract


1996-02-01 00:00:00












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    abstract::Parkinson's disease is a common neurodegenerative disorder with a mainly sporadic aetiology, although a number of monogenic familiar forms are known. Most of the motor symptoms are due to selective depletion of dopaminergic, neuromelanin-containing neurones of the substantia nigra pars compacta. Neuromelanin is the da...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Fasano M,Bergamasco B,Lopiano L

    更新日期:2006-02-01 00:00:00

  • Variables influencing the effect of a meal on brain tryptophan.

    abstract::Previous work from our laboratory points to plasma free tryptophan being a useful predictor of brain tryptophan concentration in many circumstances. Other work, in particular various studies on the acute effects of food intake, has emphasized the roles of plasma total tryptophan and of plasma large neutral amino acids...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sarna GS,Kantamaneni BD,Curzon G

    更新日期:1985-05-01 00:00:00

  • Ca2+- and calmodulin-dependent phosphorylation of microtubule-associated protein 2 and tau factor, and inhibition of microtubule assembly.

    abstract::Microtubule-associated proteins (MAPs) were phosphorylated by a Ca2+- and calmodulin-dependent protein kinase from rat brain cytosol. The maximal amount of phosphate incorporated into MAPs was 25 nmol of phosphate/mg protein. A Ka value of the enzyme for calmodulin was 57.0 nM, with MAPs as substrates. Among MAPs, MAP...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yamamoto H,Fukunaga K,Tanaka E,Miyamoto E

    更新日期:1983-10-01 00:00:00

  • Tumor necrosis factor-alpha and basic fibroblast growth factor decrease glial fibrillary acidic protein and its encoding mRNA in astrocyte cultures and glioblastoma cells.

    abstract::Tumor necrosis factor-alpha is a pluripotent cytokine that is reportedly mitogenic to astrocytes. We examined expression of the astrocyte intermediate filament component glial fibrillary acidic protein in astrocyte cultures and the U373 glioblastoma cell line after treatment with tumor necrosis factor-alpha. Treatment...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Murphy GM Jr,Lee YL,Jia XC,Yu AC,Majewska A,Song Y,Schmidt K,Eng LF

    更新日期:1995-12-01 00:00:00

  • Evidence that gabapentin reduces neuropathic pain by inhibiting the spinal release of glutamate.

    abstract::Gabapentin is an anticonvulsant that successfully treats many neuropathic pain syndromes, although the mechanism of its antihyperalgesic action remains elusive. This study aims to help delineate gabapentin's antihyperalgesic mechanisms. We assessed the effectiveness of gabapentin at decreasing mechanical and cold hype...

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    authors: Coderre TJ,Kumar N,Lefebvre CD,Yu JS

    更新日期:2005-08-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hess SD,Daggett LP,Deal C,Lu CC,Johnson EC,Veliçelebi G

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mills JC,Nelson D,Erecińska M,Pittman RN

    更新日期:1995-10-01 00:00:00

  • Ethanol-induced aspartate and taurine release from primary astrocyte cultures.

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    authors: Kimelberg HK,Cheema M,O'Connor ER,Tong H,Goderie SK,Rossman PA

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tsukada T,Watanabe M,Yamashima T

    更新日期:2001-12-01 00:00:00

  • Antiapoptotic effects of vasopressin in the neuronal cell line H32 involve protein kinase Calpha and beta.

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    authors: Chen J,Liu Y,Soh JW,Aguilera G

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Morales I,Sanchez A,Rodriguez-Sabate C,Rodriguez M

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  • Phosphorylation of mitogen-activated protein kinase in cultured rat cortical glia by stimulation of metabotropic glutamate receptors.

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    journal_title:Journal of neurochemistry

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    authors: Peavy RD,Conn PJ

    更新日期:1998-08-01 00:00:00

  • Characterization of nucleotide transport into rat brain synaptic vesicles.

    abstract::ATP transport to synaptic vesicles from rat brain has been studied using the fluorescent substrate analogue 1,N6-ethenoadenosine 5'-triphosphate (epsilon-ATP). The increase in intravesicular concentration was time dependent for the first 30 min, epsilon-ATP being the most abundant nucleotide. The complexity of the sat...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gualix J,Pintor J,Miras-Portugal MT

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  • Heat stress induces changes in protein synthesis and fast axonal transport in bullfrog sensory neurons.

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    journal_title:Journal of neurochemistry

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    authors: Stone GC,Dougher MM

    更新日期:1988-09-01 00:00:00

  • Effect of neurosteroids on the retinal gabaergic system and electroretinographic activity in the golden hamster.

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    journal_title:Journal of neurochemistry

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    authors: Sbia M,Diebler MF,Morel N,Israël M

    更新日期:1992-10-01 00:00:00

  • Hydroxylated analogues of 5-aminovaleric acid as 4-aminobutyric acidB receptor antagonists: stereostructure-activity relationships.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kristiansen U,Hedegaard A,Herdeis C,Lund TM,Nielsen B,Hansen JJ,Falch E,Hjeds H,Krogsgaard-Larsen P

    更新日期:1992-03-01 00:00:00

  • Iron deficiency alters discrete proteins in rat caudate nucleus and nucleus accumbens.

    abstract::Young rats (21 days old) made nutritionally iron deficient, by feeding them a semisynthetic diet containing skimmed milk for 5 weeks, had significantly lowered hemoglobin levels (5.2 +/- 4 g/100 ml). The nonheme iron content in caudate nucleus was decreased by 47%. The behavioral response of iron-deficient rats to apo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Youdim MB,Sills MA,Heydorn WE,Creed GJ,Jacobowitz DM

    更新日期:1986-09-01 00:00:00

  • Novel cerebrospinal fluid and serum autoantibody targets for clinically isolated syndrome.

    abstract::Limited information is available on the identity of antigens targeted by antibodies present in cerebrospinal fluid (CSF) of patients with clinically isolated syndrome (CIS). The aim of this study was to identify novel antigens for CIS and investigate their prognostic potential to predict conversion to multiple scleros...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rouwette M,Somers K,Govarts C,De Deyn PP,Hupperts R,Van Wijmeersch B,De Jong BA,Verbeek MM,Van Pesch V,Sindic C,Villar LM,Álvarez-Cermeño JC,Stinissen P,Somers V

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    journal_title:Journal of neurochemistry

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    authors: Baumgold J,Spector I

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  • Interleukin-6 activates signal transducer and activator of transcription and mitogen-activated protein kinase signal transduction pathways and induces de novo protein synthesis in human neuronal cells.

    abstract::Interleukin-6 (IL-6) is involved in the pathophysiology of various diseases of the CNS. Because the molecular mechanism of action of this cytokine in human neurons is not well understood, we were interested in characterizing and defining a model system for IL-6-induced activation of signal transduction cascades, trans...

    journal_title:Journal of neurochemistry

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    authors: Schumann G,Huell M,Machein U,Hocke G,Fiebich BL

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  • Polysialic acid limits choline acetyltransferase activity induced by brain-derived neurotrophic factor.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Burgess A,Aubert I

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  • Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chen-Roetling J,Regan RF

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  • Molecular profiles of mRNA levels in laser capture microdissected murine hippocampal regions differentially responsive to TMT-induced cell death.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lefebvre d'Hellencourt C,Harry GJ

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Guillaume D,Grisar T,Delgado-Escueta AV

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  • Inhibition of calcium-dependent ATPase from sarcoplasmic reticulum by a new class of indolizidine alkaloids, pumiliotoxins A, B, and 251D.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tamburini R,Albuquerque EX,Daly JW,Kauffman FC

    更新日期:1981-09-01 00:00:00

  • Role of glycine in the N-methyl-D-aspartate-mediated neuronal cytotoxicity.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Patel J,Zinkand WC,Thompson C,Keith R,Salama A

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  • Sequence of guinea pig myelin basic protein.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Deibler GE,Martenson RE,Krutzsch HC,Kies MW

    更新日期:1984-07-01 00:00:00

  • Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sarkar S,Cowan NJ

    更新日期:1991-08-01 00:00:00

  • Are amyloid-degrading enzymes viable therapeutic targets in Alzheimer's disease?

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Nalivaeva NN,Beckett C,Belyaev ND,Turner AJ

    更新日期:2012-01-01 00:00:00