A subtype of kappa-opioid receptor mediates inhibition of high-affinity GTPase inherent in Gi1 in guinea pig cerebellar membranes.


:The kappa-opioid receptor agonists including U-50,488H and dynorphin A (1-17) in ranges of 0.1-100 nM inhibited the hydrolysis of GTP to GDP (P(i) release) inherent in GTP-binding proteins (G proteins) in guinea pig cerebellar membranes. U-50,488H inhibited only high-affinity GTPase activity, not low-affinity activity. The action of this agonist was found to be biphasic, and there was no inhibition at concentrations > 1 microM. The inhibition was abolished by pretreatment with preactivated pertussis toxin (PTX) at concentrations > 1 micrograms/ml but not with preactivated cholera toxin (30 micrograms/ml). Similar blockade of kappa-receptor-mediated inhibition was also observed when membranes were pretreated with a low concentration (8 microM) of N-ethylmaleimide (NEM) at low temperature (4 degrees C), which alkylates the cysteine residue to be ADP-ribosylated by PTX; but this treatment caused no significant change in kappa-agonist binding. When purified Gi1, but not G(o), was reconstituted into membranes pretreated with NEM, the kappa-receptor-mediated inhibition was recovered. These findings suggest that a subtype of kappa-opioid receptor is coupled to inhibition of intrinsic activity of Gi1.


J Neurochem


Ueda H,Misawa H,Fukushima N,Katada T,Ui M,Satoh M




Has Abstract


1996-02-01 00:00:00












  • Competitive inhibition of gamma-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-e-ethanesulfonic acid and related buffers.

    abstract::Several Good buffers (MOPS, ACES, BES, HEPES, ADA, and PIPES) competitively inhibited both high-affinity and low-affinity [3H]gamma-aminobutyric acid receptor binding to rat brain synaptic membranes. The most potent inhibitor was MOPS, which had Ki values of 180 nM and 79 nM for the high- and low-affinity binding site...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tunnicliff G,Smith JA

    更新日期:1981-03-01 00:00:00

  • Social isolation stress and chronic glutathione deficiency have a common effect on the glutamine-to-glutamate ratio and myo-inositol concentration in the mouse frontal cortex.

    abstract::Environmental stress can interact with genetic predisposition to increase the risk of developing psychopathology. In this work, we tested the hypothesis that social isolation stress interacts with impaired glutathione synthesis and have cumulative effects on the neurochemical profile of the frontal cortex. A mouse mod...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Corcoba A,Gruetter R,Do KQ,Duarte JMN

    更新日期:2017-09-01 00:00:00

  • Effects of chronic ethanol consumption and aging on dopamine, serotonin, and metabolites.

    abstract::This study examined the hypothesis that chronic ethanol consumption results in significant abnormalities in both the dopaminergic and the serotonergic system of aged rats. Levels of dopamine (DA), serotonin [5-hydroxytryptamine (5-HT)], 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindole-3-acetic acid (5-HIAA)...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Woods JM,Druse MJ

    更新日期:1996-05-01 00:00:00

  • Neuronal differentiation alters the ratio of Sp transcription factors recruited to the CYP46A1 promoter.

    abstract::CYP46A1 is a neuron-specific cytochrome P450 that plays a pivotal role in maintaining cholesterol homeostasis in the CNS. However, the molecular mechanisms underlying human CYP46A1 expression are still poorly understood, partly because of the lack of a cellular model that expresses high levels of CYP46A1. Our previous...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Milagre I,Nunes MJ,Castro-Caldas M,Moutinho M,Gama MJ,Rodrigues E

    更新日期:2012-01-01 00:00:00

  • Cysteine sulfinic acid in the central nervous system: antagonistic effect of taurine on cysteine sulfinic acid-stimulated formation of cyclic AMP in guinea pig hippocampal slices.

    abstract::The stimulatory effect of cysteine sulfinic acid on cyclic AMP formation was examined in slices from three different regions of guinea pig brain. The inhibitory effect of taurine on the stimulated formation of cyclic AMP was also studied. Cysteine sulfinic acid (1--10 mM) greatly increased the cyclic AMP level in stri...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Baba A,Lee E,Tatsuno T,Iwata H

    更新日期:1982-05-01 00:00:00

  • Isolation and biochemical characterization of a neural proteoglycan expressing the L5 carbohydrate epitope.

    abstract::The monoclonal L5 antibody reacts with an N-glycosidically linked carbohydrate structure which is present on the neural cell adhesion molecule L1, neural chondroitin sulfate proteoglycans, and other not yet identified glycosylated proteins. Using this antibody, we isolated and characterized proteoglycans from adult mo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Streit A,Faissner A,Gehrig B,Schachner M

    更新日期:1990-11-01 00:00:00

  • Characterization and partial purification of AIM: a plasma protein that induces rat cerebral type 2 astroglia from bipotential glial progenitors.

    abstract::Studies on glial cultures have demonstrated that fetal bovine serum contains a factor that induces bipotential glial precursors known as oligodendrocyte-type 2 astrocyte (O-2A) progenitors to become type 2 astroglia rather than oligodendroglia. The goal of this research project was to characterize and purify this fact...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Levison SW,McCarthy KD

    更新日期:1991-09-01 00:00:00

  • Chronic morphine treatment and withdrawal increase extracellular levels of norepinephrine in the rat bed nucleus of the stria terminalis.

    abstract::Extracellular levels of norepinephrine (NE) and glutamate (Glu) in the ventral bed nucleus of the stria terminalis (vBNST) of saline- and chronic morphine-treated rats, with or without withdrawal, were studied by means of the in vivo microdialysis technique in anesthetized rats. In addition, the tissue concentration o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fuentealba JA,Forray MI,Gysling K

    更新日期:2000-08-01 00:00:00

  • Insights into the pathogenic mechanisms of Chromosome 9 open reading frame 72 (C9orf72) repeat expansions.

    abstract::The identification of a hexanucleotide repeat expansion in a non-coding region of C9orf72 as a major cause of both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) drastically changed the field of research on both of these conditions. Yet, despite the vast amount of work aimed at elucidating the m...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Todd TW,Petrucelli L

    更新日期:2016-08-01 00:00:00

  • Analysis of human neuropeptide FF gene expression.

    abstract::As an initial step to study the function of the gene encoding the human neuropeptide FF (NPFF), we cloned a 4.7-kb sequence from the promoter region. Primer extension and 5'-rapid amplification of cDNA ends revealed multiple transcription initiation sites. Northern blot analysis of the mRNA expression revealed a speci...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nystedt JM,Brandt AM,Mandelin J,Vilim FS,Ziff EB,Panula P

    更新日期:2002-09-01 00:00:00

  • Investigation of the role of conserved serine residues in the long form of the rat D2 dopamine receptor using site-directed mutagenesis.

    abstract::Three serine residues (Ser193, Ser194, Ser197) in the fifth transmembrane-spanning region of the D2 dopamine receptor have been mutated separately to alanine and the effects of the mutations determined in ligand-binding experiments with [3H] spiperone. For many antagonists the mutations had little effect, showing that...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Woodward R,Coley C,Daniell S,Naylor LH,Strange PG

    更新日期:1996-01-01 00:00:00

  • Effect of diethyl pyrocarbonate modification of benzodiazepine receptors on [3H]Ro 15-4513 binding.

    abstract::The effects of treatment of brain membranes with diethyl pyrocarbonate (DEP), a histidine-modifying reagent, on the binding of 3H-labeled Ro 15-4513 (ethyl-8-azido-5,6-dihydro-5-methyl-6-oxo-4H-imidazo[1,5-a]- [1,4]benzodiazepine-3-carboxylate) and [3H]diazepam were compared. DEP pretreatment produced a dose-dependent...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Uusi-Oukari M

    更新日期:1992-08-01 00:00:00

  • Spinal cord injury triggers systemic autoimmunity: evidence for chronic B lymphocyte activation and lupus-like autoantibody synthesis.

    abstract::Clinical and experimental data indicate that spinal cord injury (SCI) elicits pathological T-cell responses. Implicit in these data, but poorly understood, is that B lymphocytes (B cells) also contribute to the delayed pathophysiology of spinal trauma. Here, for the first time, we show that experimental spinal contusi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ankeny DP,Lucin KM,Sanders VM,McGaughy VM,Popovich PG

    更新日期:2006-11-01 00:00:00

  • Cyclic AMP-dependent positive feedback signaling pathways in the cortex contributes to visceral pain.

    abstract::Cortical areas including the anterior cingulate cortex (ACC) play critical roles in different types of chronic pain. Most of previous studies focus on the sensory inputs from somatic areas, and less information about plastic changes in the cortex for visceral pain. In this study, chronic visceral pain animal model was...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Liu SB,Wang XS,Yue J,Yang L,Li XH,Hu LN,Lu JS,Song Q,Zhang K,Yang Q,Zhang MM,Bernabucci M,Zhao MG,Zhuo M

    更新日期:2020-04-01 00:00:00

  • Altered mitochondrial respiration in selectively vulnerable brain subregions following transient forebrain ischemia in the rat.

    abstract::Mitochondrial respiratory function, assessed from the rate of oxygen uptake by homogenates of rat brain subregions, was examined after 30 min of forebrain ischemia and at recirculation periods of up to 48 h. Ischemia-sensitive regions which develop extensive neuronal loss during the recirculation period (dorsal-latera...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sims NR,Pulsinelli WA

    更新日期:1987-11-01 00:00:00

  • Altered composition of polyunsaturated fatty acyl groups in phosphoglycerides of Down's syndrome fetal brain.

    abstract::We have observed recently that in vitro lipoperoxidation is enhanced in Down's syndrome brain homogenates of prenatal age. As this may relate to the composition of polyunsaturated acyl groups (PUFA) in phospholipids, we have examined the PUFA of ethanolamine and serine phosphoglycerides (EPG and SPG), which are partic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Brooksbank BW,Martinez M,Balazs R

    更新日期:1985-03-01 00:00:00

  • pros-methylimidazoleacetic acid in rat brain: its regional distribution and relationship to metabolic pathways of histamine.

    abstract::pros-Methylimidazoleacetic acid (p-MIAA; 1-methylimidazole-5-acetic acid), an isomer of the histamine metabolite, tele-methylimidazoleacetic acid (t-MIAA), is present in brain and CSF. Its relationship to histamine synthesis and catabolism was assessed in brains of rats. p-MIAA distribution in brain regions was hetero...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Prell GD,Khandelwal JK,Hough LB,Green JP

    更新日期:1989-02-01 00:00:00

  • Differential release of endogenous 5-hydroxytryptamine, substance P, and neurokinin A from rat ventral spinal cord in response to electrical stimulation.

    abstract::The release of endogenous 5-hydroxytryptamine (5-HT), substance P (SP), and neurokinin A (NKA) from superfused tissue slices of rat ventral lumbar spinal cord, where SP and NKA coexist with 5-HT in terminals of descending bulbospinal neurons, was investigated. Electrical field stimulation was performed using square-wa...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Franck J,Brodin E,Fried G

    更新日期:1993-08-01 00:00:00

  • Effects of ATP and taurine on calcium uptake by membrane preparations of the rat retina.

    abstract::The effects of ATP and taurine on the kinetics of calcium uptake in rat retinal membrane preparations were determined. ATP increased calcium uptake at low calcium ion concentrations. Addition of ATP plus taurine further increased calcium uptake. Cooperative relationships were observed for calcium uptake in the absence...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lombardini JB

    更新日期:1983-02-01 00:00:00

  • Long-term potentiation in the dentate gyrus of the rat hippocampus is accompanied by brain-derived neurotrophic factor-induced activation of TrkB.

    abstract::A role for neurotrophic factors, in particular brain-derived neurotrophic factor (BDNF), in modulating synaptic plasticity in the adult brain has been described in recent years by several laboratories. A great deal of emphasis has been placed on establishing its precise role in the expression of long-term potentiation...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gooney M,Lynch MA

    更新日期:2001-06-01 00:00:00

  • Brain levels of NADH and NAD+ under hypoxic and hypoglycaemic conditions in vitro.

    abstract::The effects of hypoxia and hypoglycaemia on the redox state in vitro have been studied. NADH and NAD+ were extracted simultaneously from superfused cerebral cortex slices and assayed by bioluminescence. The results show a nonsignificant increase in NADH and the redox ratio in "mild hypoxia," whereas "severe hypoxia" p...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Garofalo O,Cox DW,Bachelard HS

    更新日期:1988-07-01 00:00:00

  • Regulation of neural progenitor cell development in the nervous system.

    abstract::The mammalian telencephalon, which comprises the cerebral cortex, olfactory bulb, hippocampus, basal ganglia, and amygdala, is the most complex and intricate region of the CNS. It is the seat of all higher brain functions including the storage and retrieval of memories, the integration and processing of sensory and mo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Corbin JG,Gaiano N,Juliano SL,Poluch S,Stancik E,Haydar TF

    更新日期:2008-09-01 00:00:00

  • Stimulation of excitatory amino acid release from adult mouse brain glia subcellular particles by high mobility group box 1 protein.

    abstract::The multifunctional protein high mobility group box 1 (HMGB1) is expressed in hippocampus and cerebellum of adult mouse brain. Our aim was to determine whether HMGB1 affects glutamatergic transmission by monitoring neurotransmitter release from glial (gliosomes) and neuronal (synaptosomes) re-sealed subcellular partic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pedrazzi M,Raiteri L,Bonanno G,Patrone M,Ledda S,Passalacqua M,Milanese M,Melloni E,Raiteri M,Pontremoli S,Sparatore B

    更新日期:2006-11-01 00:00:00

  • Endoplasmic reticulum calcium release is modulated by actin polymerization.

    abstract::Intracellular calcium ions regulate the structure and functions of cytoskeletal proteins. On the other hand, recent studies have shown that the cytoskeleton, and actin filaments in particular, can modulate calcium influx through plasma membrane ligand- and voltage-gated channels. We now report that calcium release fro...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang Y,Mattson MP,Furukawa K

    更新日期:2002-08-01 00:00:00

  • Hypoxia dramatically increases the nonspecific transport of blood-borne proteins to the brain.

    abstract::Increased cerebrovascular permeability is an important factor for the development of cerebral edema. To investigate the effect of hypoxia on the transport of blood-borne proteins to the brain, we used a cell culture model of the blood-brain barrier (BBB) consisting of a coculture of brain capillary endothelial cells a...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Plateel M,Teissier E,Cecchelli R

    更新日期:1997-02-01 00:00:00

  • Expression and evolution of the mammalian brain gene Ttyh1.

    abstract::Homologues of the Drosophila melanogaster tweety (tty) gene are present in mammals and Caenorhabditis elegans. The encoded proteins have five predicted membrane-spanning regions and recent findings suggest that some family members may be chloride channels. Phylogenetic analysis of the tty family including novel member...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Matthews CA,Shaw JE,Hooper JA,Young IG,Crouch MF,Campbell HD

    更新日期:2007-02-01 00:00:00

  • Novel photoactivatable agonist of the nicotinic acetylcholine receptor of potential use for exploring the functional activated state.

    abstract::The nicotinic acetylcholine receptor (AChR) exhibits at least four different conformational states varying in affinity for agonists such as acetylcholine (ACh). Photoaffinity labeling has been previously used to elucidate the topography of the AChR. However, to date, the photosensitive probes used to explore the choli...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kotzyba-Hibert F,Kessler P,Zerbib V,Bogen C,Snetkov V,Takeda K,Goeldner M,Hirth C

    更新日期:1996-12-01 00:00:00

  • Presence of alpha7 nicotinic acetylcholine receptors on dorsal root ganglion neurons proved using knockout mice and selective alpha-neurotoxins in histochemistry.

    abstract::In complex tissues where multiple subtypes of nicotinic acetylcholine receptors (nAChRs) are expressed, immunohistochemistry has been the most popular tool for investigation of nAChR subunit distribution. However, recent studies with nAChR subunit knockout mice demonstrated that a large panel of antibodies is unsuitab...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shelukhina IV,Kryukova EV,Lips KS,Tsetlin VI,Kummer W

    更新日期:2009-05-01 00:00:00

  • Human brain beta A4 amyloid protein precursor of Alzheimer's disease: purification and partial characterization.

    abstract::The major component of the amyloid deposition that characterizes Alzheimer's disease is the 4-kDa beta A4 protein, which is derived from a much larger amyloid protein precursor (APP). A procedure for the complete purification of APP from human brain is described. The same amino terminal sequence of APP was found in tw...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Moir RD,Martins RN,Bush AI,Small DH,Milward EA,Rumble BA,Multhaup G,Beyreuther K,Masters CL

    更新日期:1992-10-01 00:00:00

  • Stimulation of dopamine release from cultured rat mesencephalic cells by naturally occurring excitatory amino acids: involvement of both N-methyl-D-aspartate (NMDA) and non-NMDA receptor subtypes.

    abstract::In rat mesencephalic cell cultures, L-glutamate at concentrations ranging from 100 microM to 1 mM stimulated release of [3H]dopamine that was attenuated by the non-N-methyl-D-aspartate (non-NMDA) receptor antagonist 6,7-dinitroquinoxalinedione, but not by the selective NMDA receptor antagonists (+)-5-methyl-10,11-dihy...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mount H,Quirion R,Chaudieu I,Boksa P

    更新日期:1990-07-01 00:00:00