当前位置： SCI文献检索 > JOURNAL OF NEUROCHEMISTRY期刊下所有文献 > Accumulation of DNA damage in aging neurons occurs through a mechanism other than apoptosis.

Accumulation of DNA damage in aging neurons occurs through a mechanism other than apoptosis.

Abstract：

:Two biochemical strategies using nick translation-type of incubation and terminal tranferase-catalyzed reaction were used to assess single-(SSB) and double-strand (DSB) breaks in DNA of permeabilized neurons isolated from young, adult, and old rat cerebral cortex. Both SSBs and DSBs accumulate with age. On prior treatment of neuronal cells with 1 mM glutamate or 50 microM N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), more extensive damage was seen at all ages, with the old neurons suffering maximal damage. When neuronal DNA was subjected to agarose electrophoresis, increasingly diffused bands were seen with age in normally aging neurons. However, a typical nucleosomal ladder, characteristic of apoptosis, was seen only when the cells were exposed to either glutamate or MNNG irrespective of the age of the neurons. Furthermore, this apoptotic fragmentation of DNA was prevented by prior treatment of the cells with either cycloheximide or aurintricarboxylic acid, indicating that both glutamate and MNNG induce programmed cell death. Fluorescence microscopic observation of glutamate- and MNNG-treated neurons after acridine orange staining revealed a high degree of staining and marked condensation of nuclear DNA. On the other hand, no such phenomenon was observed in normally aging neurons either histologically or in biochemical assays of damage. It is concluded that both glutamate and MNNG induce programmed cell death in neurons independent of age and that accumulation of DNA damage in naturally aging neurons occurs through a process other than that of apoptosis.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Mandavilli BS,Rao KS

doi

10.1046/j.1471-4159.1996.67041559.x

subject

Has Abstract

pub_date

1996-10-01 00:00:00

pages

1559-65

issue

4

eissn

0022-3042

issn

1471-4159

journal_volume

67

pub_type

杂志文章

相关文献

JOURNAL OF NEUROCHEMISTRY文献大全
  • Effects of ischemic post-conditioning on neuronal VEGF regulation and microglial polarization in a rat model of focal cerebral ischemia.

    abstract：:Ischemic postconditioning is increasingly being investigated as a therapeutic approach for cerebral ischemia. However, the majority of studies are focused on the acute protection of neurons per se. Whether and how postconditioning affects multiple cells in the recovering neurovascular unit remains to be fully elucidat...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.14337

    authors： Esposito E,Hayakawa K,Ahn BJ,Chan SJ,Xing C,Liang AC,Kim KW,Arai K,Lo EH

    更新日期：2018-07-01 00:00:00

  • Inhibition of insulin-like growth factor-1 receptor and IRS-2 signaling by ethanol in SH-SY5Y neuroblastoma cells.

    abstract：:The effect of ethanol on insulin-like growth factor-1 (IGF-I)-mediated signal transduction and functional activation in neuronal cells was examined. In human SH-SY5Y neuroblastoma cells, ethanol inhibited tyrosine autophosphorylation of the IGF-I receptor. This corresponded to the inhibition of IGF-I-induced phosphory...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00025.x

    authors： Seiler AE,Ross BN,Rubin R

    更新日期：2001-01-01 00:00:00

  • Effects of an extract of Ginkgo biloba on the 3',5'-cyclic AMP phosphodiesterase activity of the brain of normal and triethyltin-intoxicated rats.

    abstract：:For clarification of the beneficial effects of the extract of Ginkgo biloba (EGB) on triethyltin (TET) toxicity in rats, the phosphodiesterase (PDE) activities of the cerebral tissue were measured under in vitro and ex vivo conditions. Under in vitro conditions, low concentrations of EGB (0.25-4.0 mg/L) activated the ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1987.tb03401.x

    authors： Macovschi O,Prigent AF,Nemoz G,Pacheco H

    更新日期：1987-07-01 00:00:00

  • The role of exercise in mitigating subcortical ischemic vascular cognitive impairment.

    abstract：:Subcortical ischemic vascular cognitive impairment (SIVCI) is the most preventable form of cognitive dysfunction. There is converging evidence from animal and human studies that indicate vascular injury as the primary cause of SIVCI. Currently, there are no curative pharmaceutical treatments for vascular dementia; how...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章,评审

    doi：10.1111/jnc.14153

    authors： Dao E,Hsiung GR,Liu-Ambrose T

    更新日期：2018-03-01 00:00:00

  • Circadian rhythm in pineal methionine S-adenosyltransferase.

    abstract：:The circadian rhythm of methionine S-adenosyltransferase, which catalyzes the formation of S-adenosylmethionine, a cosubstrate for melatonin in the pineal gland, follows the pattern of hydroxyindole-O-methyltransferase. Around the middle of the dark period, methionine S-adenosyltransferase and hydroxyindole-O-methyltr...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb10840.x

    authors： Guchhait RB,Monjan AA

    更新日期：1981-06-01 00:00:00

  • Molecular dissection of native mammalian forebrain NMDA receptors containing the NR1 C2 exon: direct demonstration of NMDA receptors comprising NR1, NR2A, and NR2B subunits within the same complex.

    abstract：:The subunit compositions of the NR1 C2 exon-containing N-methyl-D-aspartate (NMDA) receptors of adult mammalian forebrain were determined by using a combination of immunoaffinity chromatography and immunoprecipitation studies with NMDA receptor subunit-specific antibodies. NMDA receptors were solubilised by sodium deo...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.69052138.x

    authors： Chazot PL,Stephenson FA

    更新日期：1997-11-01 00:00:00

  • Interaction of opiates with opioid binding sites in the bovine adrenal medulla: II. Interaction with kappa sites.

    abstract：:In this study we examined the interaction of opiates with kappa binding sites in the bovine adrenal medulla. [3H]Ethylketocyclazocine (EKC), [3H]etorphine, and [3H]bremazocine stereoselective bindings were used to assay these interactions. The kappa sites were found to be heterogeneous: [3H]bremazocine identified with...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1985.tb04047.x

    authors： Castanas E,Bourhim N,Giraud P,Boudouresque F,Cantau P,Oliver C

    更新日期：1985-09-01 00:00:00

  • Systemically administered neuregulin-1β1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease.

    abstract：:Previously, we demonstrated that systemically injected extracellular domain of neuregulin-1β1 (Nrg1β1), a nerve growth and differentiation factor, passes the blood-brain barrier and rescues dopaminergic neurons of substantia nigra in the 6-hydroxydopamine-mouse model of Parkinson's disease (PD). Here, we studied the e...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.13026

    authors： Depboylu C,Rösler TW,de Andrade A,Oertel WH,Höglinger GU

    更新日期：2015-05-01 00:00:00

  • Increased extracellular serotonin in rat brain after systemic or intraraphe administration of morphine.

    abstract：:The effect of morphine on serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in the CNS of unanesthetized rats was investigated by microdialysis. Morphine was administered either subcutaneously, by local perfusion into the diencephalon, or by intraraphe microinjection. Systemic administration of morphine resulte...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.63020517.x

    authors： Tao R,Auerbach SB

    更新日期：1994-08-01 00:00:00

  • Glucose transport and utilization are altered in the brain of rats deficient in n-3 polyunsaturated fatty acids.

    abstract：:Long-chain polyunsaturated (n-3) fatty acids have been reported to influence the efficiency of membrane receptors, transporters and enzymes. Because the brain is particularly rich in docosahexaenoic acid (DHA, 22:6 n-3), the present study addresses the question of whether the 22:6 n-3 fatty acid deficiency induces dis...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2002.00932.x

    authors： Ximenes da Silva A,Lavialle F,Gendrot G,Guesnet P,Alessandri JM,Lavialle M

    更新日期：2002-06-01 00:00:00

  • Potassium- and capsaicin-induced release of agmatine from spinal nerve terminals.

    abstract：:Agmatine (decarboxylated arginine) was originally identified in the CNS as an imidazoline receptor ligand. Further studies demonstrated that agmatine antagonizes NMDA receptors and inhibits nitric oxide synthase. Intrathecally administered agmatine inhibits opioid tolerance and hyperalgesia evoked by inflammation, ner...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2007.04647.x

    authors： Goracke-Postle CJ,Overland AC,Riedl MS,Stone LS,Fairbanks CA

    更新日期：2007-09-01 00:00:00

  • gdnf affects early diencephalic dopaminergic neuron development through regulation of differentiation-associated transcription factors in zebrafish.

    abstract：:Glial cell line-derived neurotrophic factor (GDNF) has been reported to enhance dopaminergic neuron survival and differentiation in vitro and in vivo, although those results are still being debated. Glial cell line-derived neurotrophic factor (gdnf) is highly conserved in zebrafish and plays a role in enteric nervous ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.15108

    authors： Wong CED,Hua K,Monis S,Saxena V,Norazit A,Noor SM,Ekker M

    更新日期：2020-06-24 00:00:00

  • Syntaxin 1B regulates synaptic GABA release and extracellular GABA concentration, and is associated with temperature-dependent seizures.

    abstract：:De novo heterozygous mutations in the STX1B gene, encoding syntaxin 1B, cause a familial, fever-associated epilepsy syndrome. Syntaxin 1B is an essential component of the pre-synaptic neurotransmitter release machinery as a soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein that regulates th...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.15159

    authors： Mishima T,Fujiwara T,Kofuji T,Saito A,Terao Y,Akagawa K

    更新日期：2020-08-28 00:00:00

  • Highly sensitive assay for dopamine-beta-hydroxylase activity in human cerebrospinal fluid by high performance liquid chromatography-electrochemical detection: properties of the enzyme.

    abstract：:This paper describes a new, sensitive assay for dopamine-beta-hydroxylase (DBH) activity in human cerebrospinal fluid (CSF), serum and brain tissues by high performance liquid chromatography (HPLC) with electrochemical detection (ED). Dopamine (DA) was used as a substrate and was incubated under optimal conditions. No...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb00454.x

    authors： Matsui H,Kato T,Yamamoto C,Fujita K,Nagatsu T

    更新日期：1981-08-01 00:00:00

  • Changes in lipogenic capacity and activities of ketolytic and lipogenic enzymes in brain regions of developing rats.

    abstract：:Oxidation of ketone bodies (KBs) generates acetyl coenzyme A (AcCoA), which can be further incorporated into fatty acid. We have determined the rates of lipogenesis from ketone bodies in developing rats and their relation to the activities of enzymes involved in the production of cytoplasmic AcCoA via different pathwa...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb12658.x

    authors： Yeh YY,Ginsburg JR,Tso TB

    更新日期：1983-01-01 00:00:00

  • Protein kinase inhibitor H-7 differentially affects early and delayed nerve growth factor responses in PC12 cells.

    abstract：:The effects of the protein kinase inhibitor H-7 on early and delayed responses to nerve growth factor (NGF) were investigated in PC12 cells. H-7 reduced the NGF-induced expression of c-Fos in a dose-dependent manner without affecting the time course of c-Fos appearance. Conversely, H-7 potentiated delayed NGF effects,...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.62020479.x

    authors： Kahle P,Mangold M,Kuwahara T,Schubenel R,Hertel C

    更新日期：1994-02-01 00:00:00

  • Modelling Parkinson-like neurodegeneration via osmotic minipump delivery of MPTP and probenecid.

    abstract：:Mouse models of MPTP intoxication have been used extensively to explore the molecular mechanisms of Parkinson's disease. However, these models present some limitations since; (i) Dopaminergic (DA) cell death occurs rapidly in contrast to the presumably slow evolution of the disease process. (ii) Some of the key histol...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2008.05651.x

    authors： Alvarez-Fischer D,Guerreiro S,Hunot S,Saurini F,Marien M,Sokoloff P,Hirsch EC,Hartmann A,Michel PP

    更新日期：2008-11-01 00:00:00

  • Cloning and characterization of the murine Imitation Switch (ISWI) genes: differential expression patterns suggest distinct developmental roles for Snf2h and Snf2l.

    abstract：:Here we report the cloning of two cDNAs, Snf2h and Snf2l, encoding the murine members of the Imitation Switch (ISWI) family of chromatin remodeling proteins. To gain insight into their function we examined the spatial and temporal expression patterns of Snf2h and Snf2l during development. In the brain, Snf2h is preval...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00324.x

    authors： Lazzaro MA,Picketts DJ

    更新日期：2001-05-01 00:00:00

  • Dermorphins, opioid peptides from amphibian skin, act on opioid receptors of mouse neuroblastoma x rat glioma hybrid cells.

    abstract：:Dermorphin and its Hyp6 analogue are opiate-like heptapeptides originally discovered in frog skin and characterized by the presence of a D-Ala2 residue in their sequence. They were assayed for their capacity to compete with [3H]Leu-enkephalin for binding to opioid receptors in membranes of neuroblastoma x glioma hybri...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb06336.x

    authors： Glaser T,Hübner K,de Castiglione R,Hamprecht B

    更新日期：1981-12-01 00:00:00

  • Chronic NMDA administration to rats up-regulates frontal cortex cytosolic phospholipase A2 and its transcription factor, activator protein-2.

    abstract：:Excessive N-methyl-D-aspartate (NMDA) signaling is thought to contribute to bipolar disorder symptoms. Lithium and carbamazepine, effective against bipolar mania, are reported in rats to reduce brain transcription of an arachidonic acid selective calcium-dependent cytosolic phospholipase A(2) (cPLA(2)), as well as exp...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章,收录出版

    doi：10.1111/j.1471-4159.2007.04648.x

    authors： Rao JS,Ertley RN,Rapoport SI,Bazinet RP,Lee HJ

    更新日期：2007-09-01 00:00:00

  • A comparison of two alternatively spliced forms of a metabotropic glutamate receptor coupled to phosphoinositide turnover.

    abstract：:A comparison of the pharmacological and physiological properties of the metabotropic glutamate 1 alpha and 1 beta receptors (mGluR1 alpha and mGluR1 beta) expressed in baby hamster kidney (BHK 570) cells was performed. The mGluR1 beta receptor is an alternatively spliced form of mGluR1 alpha with a modified carboxy te...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1993.tb03540.x

    authors： Pickering DS,Thomsen C,Suzdak PD,Fletcher EJ,Robitaille R,Salter MW,MacDonald JF,Huang XP,Hampson DR

    更新日期：1993-07-01 00:00:00

  • Serine transhydroxymethylase activity in vertebrate retina.

    abstract：:The presence of serine transhydroxymethylase (STHM; EC 2.1.2.1) in crude homogenates of rat, guinea pig and goat retina has been demonstrated. There was a variation in the levels of retinal STHM activity in the three species. Substantial STHM activity was found in all the three retinal preparations in the absence of p...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1982.tb07955.x

    authors： Dasgupta P,Narayanaswami A

    更新日期：1982-09-01 00:00:00

  • Reduced glycine stimulation of [3H]MK-801 binding in Alzheimer's disease.

    abstract：:The novel N-methyl-D-aspartate receptor channel ligand (+)-[3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5, 10-imine maleate ([3H]MK-801) has been utilized to label this receptor in human brain tissue. Characteristics of [3H]MK-801 binding to well-washed membranes from 17 control subjects and 16 patients with...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1989.tb11760.x

    authors： Procter AW,Wong EH,Stratmann GC,Lowe SL,Bowen DM

    更新日期：1989-09-01 00:00:00

  • Antisense strategy unravels a dopamine receptor distinct from the D2 subtype, uncoupled with adenylyl cyclase, inhibiting prolactin release from rat pituitary cells.

    abstract：:The antisense strategy was used to unravel the functional contribution of the mRNAs encoding dopamine (DA) receptors to the multiple transduction mechanisms operated by DA in rat pituitary cells. An antisense oligonucleotide was designed to recognize seven nucleotides upstream and 11 nucleotides downstream from the in...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.62041260.x

    authors： Valerio A,Alberici A,Tinti C,Spano P,Memo M

    更新日期：1994-04-01 00:00:00

  • Proteomic analysis of rat hippocampal plasma membrane: characterization of potential neuronal-specific plasma membrane proteins.

    abstract：:The hippocampus is a distinct brain structure that is crucial in memory storage and retrieval. To identify comprehensively proteins of hippocampal plasma membrane (PM) and detect the neuronal-specific PM proteins, we performed a proteomic analysis of rat hippocampus PM using the following three technical strategies. F...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2006.03934.x

    authors： Chen P,Li X,Sun Y,Liu Z,Cao R,He Q,Wang M,Xiong J,Xie J,Wang X,Liang S

    更新日期：2006-08-01 00:00:00

  • Transport of prostaglandins and other eicosanoids by the choroid plexus: its characterization and physiological significance.

    abstract：:Choroid plexi from the lateral ventricles of rabbits, cats, and dogfish (Mustelus canis) were used to characterize the prostaglandin (PG) uptake process and to establish its kinetic parameters and substrate specificity. The apparent Kt for PGF2 alpha transport by the rabbit choroid plexus was 20 microM; the Jmax was 2...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1986.tb08490.x

    authors： DiBenedetto FE,Bito LZ

    更新日期：1986-06-01 00:00:00

  • Trichloroethylene induces dopaminergic neurodegeneration in Fisher 344 rats.

    abstract：:Trichloroethylene, a chlorinated solvent widely used as a degreasing agent, is a common environmental contaminant. Emerging evidence suggests that chronic exposure to trichloroethylene may contribute to the development of Parkinson's disease. The purpose of this study was to determine if selective loss of nigrostriata...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2009.06497.x

    authors： Liu M,Choi DY,Hunter RL,Pandya JD,Cass WA,Sullivan PG,Kim HC,Gash DM,Bing G

    更新日期：2010-02-01 00:00:00

  • Modulation of serotonin transporter activity by a protein kinase C activator and an inhibitor of type 1 and 2A serine/threonine phosphatases.

    abstract：:We studied the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), a protein kinase C (PKC) activator, and calyculin A (CLA), an inhibitor of type 1 and 2A serine/threonine phosphatases, on serotonin uptake by a human placenta choriocarcinoma cell line (BeWo) and COS-7 cells expressing recombinant serotonin transpo...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.68062618.x

    authors： Sakai N,Sasaki K,Nakashita M,Honda S,Ikegaki N,Saito N

    更新日期：1997-06-01 00:00:00

  • Glucose modulates the release of histamine from the mouse hypothalamus in vitro.

    abstract：:The effect of glucose concentration on the in vitro release of histamine (HA) was examined, using two different preparations of the mouse hypothalamus. The HA and tele-methylhistamine released from whole blocks of the hypothalamus into the medium linearly increased during 2-h incubation in normal Krebs-Ringer bicarbon...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1986.tb13086.x

    authors： Nishibori M,Oishi R,Itoh Y,Saeki K

    更新日期：1986-12-01 00:00:00

  • Protective effects of extracellular acidosis and blockade of sodium/hydrogen ion exchange during recovery from metabolic inhibition in neuronal tissue culture.

    abstract：:Acidosis is a universal response of tissue to ischemia. In the brain, severe acidosis has been linked to worsening of cerebral infarction. However, milder acidosis can have protective effects. As part of our investigations of the therapeutic window in our neuronal tissue culture model of ischemia, we investigated the ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1996.67062379.x

    authors： Vornov JJ,Thomas AG,Jo D

    更新日期：1996-12-01 00:00:00