Abstract：
:Pulsed electric fields (PEFs) are applied as physical stimuli for DNA/drug delivery, cancer therapy, gene transformation, and microorganism eradication. Meanwhile, calcium electrotransfer offers an interesting approach to treat cancer, as it induces cell death easier in malignant cells than in normal cells. Here, we study the spatial and temporal cellular responses to 10 μs duration PEFs; by observing real-time, the uptake of extracellular calcium through the cell membrane. The experimental setup consisted of an inverted fluorescence microscope equipped with a color high-speed framing camera and a specifically designed miniaturized pulsed power system. The setup allowed us to accurately observe the permeabilization of HeLa S3 cells during application of various levels of PEFs ranging from 0.27 to 1.80 kV/cm. The low electric field experiments confirmed the threshold value of transmembrane potential (TMP). The high electric field observations enabled us to retrieve the entire spatial variation of the permeabilization angle (θ). The temporal observations proved that after a minimal permeabilization of the cell membrane, the ionic diffusion was the prevailing mechanism of the delivery to the cell cytoplasm. The observations suggest 0.45 kV/cm and 100 pulses at 1 kHz as an optimal condition to achieve full calcium concentration in the cell cytoplasm. The results offer precise levels of electric fields to control release of the extracellular calcium to the cell cytoplasm for inducing minimally invasive cancer calcium electroporation, an interesting affordable method to treat cancer patients with minimum side effects.
journal_name
Drug Deliv Transl Resjournal_title
Drug delivery and translational researchauthors
Guionet A,Moosavi Nejad S,Teissié J,Sakugawa T,Katsuki S,Akiyama H,Hosseini Hdoi
10.1007/s13346-018-0533-5subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1152-1161issue
5eissn
2190-393Xissn
2190-3948pii
10.1007/s13346-018-0533-5journal_volume
8pub_type
杂志文章abstract：:The objective of this research was to develop polymeric micellar formulations of inhibitors of signal transducer and activator of transcription 3 (STAT3) dimerization, i.e., S3I-1757 and S3I-201, and evaluate the activity of successful formulations in B16-F10 melanoma, a STAT3 hyperactive cancer model, in vitro and in...
journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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journal_title：Drug delivery and translational research
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更新日期：2020-06-25 00:00:00
abstract：:Dilauryl fluorescein (DLF) is a lipid soluble molecule that becomes fluorescent when lauric acid is removed by hydrolysis The purpose of these studies was to evaluate DLF as a potential probe for the function of reconstituted high-density lipoproteins (rHDL) as hydrophobic drug transport vehicles. The DLF containing r...
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