Abstract:
:Acquired chemoresistance refers to tumor cells gradually losing their sensitivity to anticancer drugs during the course of treatment, resulting in tumor progression or recurrence. This phenomenon, which has deleterious outcomes for the patient, has long been observed in patients with glioblastoma receiving temozolomide (TMZ)-based radiochemotherapy. Currently, the mechanisms for acquired TMZ chemoresistance are not fully understood. In the present study, a TMZ-resistant cell line U251R with a 4-fold 50% inhibition concentration compared with its TMZ-sensitive parent cell line was isolated by incremental long-time TMZ treatment in the human glioblastoma cell line U251. Fluorescence-activated cell sorting analysis indicated G2/M arrest and a lower proportion of cells in the S phase, accompanied by a decreased apoptosis rate in the U251R cell line compared with the parental U251 cell line. In addition, a sphere-formation assay indicated an increased self-renewal capacity in U251R cells. Furthermore, a high-throughput protein microarray unveiled more than 200 differentially expressed proteins as potential molecular targets accounting for acquired TMZ resistance. Subsequent bioinformatics analysis illustrated the molecular and signaling networks and revealed the central role of SRC. Immunoblotting and reverse-transcription quantitative polymerase chain reaction analysis further confirmed the expressional upregulation of SRC family kinases. Moreover, SRC knockdown led to partial reversal of TMZ resistance in the U251R cell line and sensitization in the U373 cell line. These data helped to develop a comprehensive understanding of survival strategies, particularly with respect to pro-stemness regulation, which could be potential targets for overcoming TMZ resistance.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Lin J,Zuo J,Cui Y,Song C,Wu X,Feng H,Li J,Li S,Xu Q,Wei W,Qiu G,He Hdoi
10.3892/or.2018.6322subject
Has Abstractpub_date
2018-05-01 00:00:00pages
2333-2341issue
5eissn
1021-335Xissn
1791-2431journal_volume
39pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::The number of new lung cancer cases diagnosed yearly is high, and the mortality rate has not substantially declined. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, and adenocarcinoma accounts for the largest proportion of NSCLC. Currently, platinum-based combined chemotherapy, particularly ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5505
更新日期:2017-04-01 00:00:00
abstract::The objective of the study was to investigate the dynamic changes of cortisol and soluble TNF-alpha p-55 and p-75 receptors in serum of advanced cancer patients during 24 h. The examined group consisted of 42 patients suffering from advanced gastrointestinal neoplasms (colorectal, gastric and pancreatic cancer). Serum...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.1.207
更新日期:2001-01-01 00:00:00
abstract::Resistance to etoposide has been associated with the overexpression of P-glycoprotein and MRP1 in human tumor cells. However, the role of BCRP in resistance to etoposide has not been clearly established, especially the significance of arginine 482 mutations in drug transport (cellul...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1468
更新日期:2012-01-01 00:00:00
abstract::EVL belongs to the Ena/VASP (Enabled/vasodilator-stimulated phosphoprotein) family of proteins [Mena (mammalian Ena), VASP and EVL], which have a range of roles in regulating the actin cytoskeleton. Growing evidence suggests that Mena and VASP are involved in carcinogenesis, though little is known about the significan...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Multicellular tumor spheroids have been used to examine aspects of combined modality treatment since they often recreate the in vivo tumor environment much more closely than other models. The radioenhancement by gemcitabine (dFdC) on human glioma spheroids derived from cell lines (CLS) and biopsy tissue, grown as orga...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The global rising incidence of hepatocellular carcinoma (HCC), which parallels the increase of hepatitis C virus (HCV) prevalence, has sparked a renewed interest in discovering additional HCC serum markers. In this study, we investigated the clinical use of serum E-cadherin, ICAM, MMP-2, VEGF, OPN and β-catenin as pot...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1355
更新日期:2011-10-01 00:00:00
abstract::The goal of this study was to evaluate the effect of the Na+/H+ exchanger-1 (NHE-1) antisense gene on drug-resistant human small cell lung cancer (SCLC) cell proliferation and apoptosis. A recombinant NHE-1 antisense gene was transfected into drug-resistant human SCLC H446/CDDP cells. Intracellular pH (pHi) was measur...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000347
更新日期:2009-05-01 00:00:00
abstract::Selenium has been associated with anticancer activity by affecting multiple cellular processes. We reasoned that the simultaneous modulation of multiple radioresponse regulators by selenium should increase radiosensitivity if selenium is combined with radiation in cancer therapy. Therefore, we explored the possibility...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Recently, overexpression of EphA2, a member of the Eph family of receptor tyrosine kinases, has been reported in several cancers. Reduced expression of E-cadherin, an intercellular adhesion molecule of epithelial cells, has been reported to be associated with aggressive clinicopathological phenotypes in various cancer...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::A growing number of studies suggest that the hepatitis B virus X protein (HBx) enhances the protein stability of the hypoxia-inducible factor-1α (HIF-1α). However, the relationship between hepatitis B virus (HBV), HBx and hypoxia-inducible factor-2α (HIF-2α) has not yet been fully elucidated. Immunohistochemical analy...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4480
更新日期:2016-03-01 00:00:00
abstract::We have recently noticed an accidental error in part of a figure which appeared in the above‑mentioned article. In Fig. 3A, the image for the HGC27‑pEF, 15 h panel was mistakenly replicated as the HGC27‑KD, 0 h panel in the same figure, and the AGS‑pEF, 15 h and AGS KD, 0 h panels were mistakenly switched with each ot...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6269
更新日期:2018-04-01 00:00:00
abstract::Apoptosis is a crucial event for anticancer drug efficacy. The signal pathways activated by anticancer drugs are classified as death receptor (DR) -dependent or -independent. The mechanisms by which anticancer drugs induce apoptosis via DR-dependent pathways are not fully understood. In the present study, we assessed ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Gelatinases play an important role in tumor growth and metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumors. Lidamycin is an enediyne antitumor antibiotic with potent cytotoxicity. We previously reported that a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1910
更新日期:2012-10-01 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC) is the predominant subtype of human esophageal cancer in East Asia. In the present study, we explored the tumor suppressive function of microRNA-181d (miR-181d) in ESCC. Quantitative RT-PCR was used to assess gene levels of miR‑181d in both ESCC cell lines and clinical samples...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5028
更新日期:2016-10-01 00:00:00
abstract::A 69-year-old woman presented to her local clinic with vomiting and abdominal distension. Since a bowel obstruction by left colon cancer was suspected due to a marked dilation of the transverse colon, she was referred to our hospital. On admission, an enema disclosed a complete obstruction at the splenic flexure of th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Hypoxia was shown to increase tumor cell invasion into the extracellular matrix in vitro. This result suggests that heparanase (Hpa), one of the key enzymes involved in tumor invasion and metastasis, may be regulated by hypoxia. RT-PCR, Western blot and Matrigel invasive assays were used to study the regulation of Hpa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is a key kinase acting downstream of growth factor receptor PI3K and AKT signaling, leading to processes resulting in increased cell size and proliferation through translation control. Rapamycin, a specific inhibitor of mTOR, results predomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1855
更新日期:2012-09-01 00:00:00
abstract::SET domain bifurcated 1 (SETDB1) is a histone H3 lysine 9 methyltransferase that is highly expressed in various tumor types, including breast cancer. However, how SETDB1 functions in breast cancer is unclear. In the present study, proliferation, migration and invasion assays were performed to explore the role of SETDB...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6871
更新日期:2019-02-01 00:00:00
abstract::Tongue cancer is one of the most common types of cancer, but its molecular etiology and pathogenesis remain unclear. The aim of the present study was to elucidate the pathogenesis of tongue cancer and investigate novel potential diagnostic and therapeutic targets. Four matched pairs of tongue cancer and paracancerous ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7560
更新日期:2020-06-01 00:00:00
abstract::Androgen receptor (AR) expression in prostate cancer (CaP) cells varies due to the multiple changes including epigenetic modifications such as DNA methylation and histone deacetylation. DNA methyltransferase and histone deacetylase inhibitors are promising for the treatment of CaP. The aim of our study was to analyze ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2344
更新日期:2013-05-01 00:00:00
abstract::Mitogene-inducible gene-6 (Mig-6) is involved in the intracellular signaling pathway as an adaptor molecule. Mig-6 expression is rapidly induced upon various kinds of mitogenic and stressful stimulations. We previously demonstrated that Mig-6 expression is induced by activated Ki-ras in human colon cancer cells and th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::Reactive oxygen species (ROS) can drive the de‑differentiation of tumor cells leading to the process of epithelial-to-mesenchymal transition (EMT) to enhance invasion and metastasis. The invasive and metastatic phenotype of malignant cells is often linked to loss of E-cadherin expression, a hallmark of EMT. Recent stu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3448
更新日期:2014-11-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a major health problem worldwide. CD147 has been reported to be overexpressed in HCC and blocking CD147 expression can decrease tumor growth. (131)I is often used in combination with other drugs to treat HCC and yields positive results. In this study, we combined the (131)I and CD147 ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2418
更新日期:2013-07-01 00:00:00
abstract::Garlic and its constituents are reported to have a preventive effect against colorectal cancer in animal models. Aged garlic extract (AGE), which is produced by natural extraction from fresh garlic for more than 10 months in aqueous ethanol, also has reputed chemopreventive effects on colon carcinogenesis, but has nev...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3705
更新日期:2015-03-01 00:00:00
abstract::The 5-year survival rate of nasopharyngeal carcinoma (NPC) is still disappointing despite the much improved technologies in its treatment. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) can selectively induce apoptosis in most tumor cells while sparing normal cells....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1423
更新日期:2011-12-01 00:00:00
abstract::Emerging evidence suggests that cancer cells present profound epigenetic alterations in addition to featuring classic genetic mutations. Valproic acid (VPA), a histone deacetylase inhibitor, can potently inhibit tumor growth and induce differentiation. However, the effect and underlying mechanism of VPA on head and ne...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4145
更新日期:2015-10-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a ubiquitous pathogen that was first identified as a human cancer virus. Many human cancers are associated with EBV, and we demonstrated that EBV infects macrophages. Macrophages infected with EBV show a close correlation with many human cancers, and thus more attention must be given to the...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2014.3475
更新日期:2014-11-01 00:00:00
abstract::Frizzled2 (Fzd2) is a receptor for wingless-type MMTV integration site family members (Wnts), the aberrant overexpression of which has been noted to contribute to cancer metastasis. The present study was performed to characterize the role of Fzd2 in the migration and invasion of oral squamous cell carcinomas (OSCC) in...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4285
更新日期:2015-12-01 00:00:00
abstract::Splenic metastasis from a lung cancer is infrequently noted, however, the pattern of the metastasis has not been studied, thus we conducted a retrospective study. Twelve of the consecutive 997 patients with lung cancer, who were admitted to our division, developed splenic metastasis. The pattern of splenic metastasis ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.6.1239
更新日期:2001-11-01 00:00:00
abstract::The Bcl10 gene was recently isolated from the breakpoint region of t(1;14)(p22;q32) in mucosa-associated lymphoid tissue (MALT) lymphomas. Somatic mutations of Bcl10 were found in not only t(1;14)-bearing MALT lymphomas, but also a wide range of other tumors. To clarify the actual frequency and spectrum of Bcl10 mutat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.6.1333
更新日期:2001-11-01 00:00:00