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The influence of selenium on expression levels of the rbcL gene in Chlorella vulgaris.

Abstract：

:In this study, the effects of selenium on the microalgae Chlorella vulgaris were examined. Four groups of C. vulgaris were cultivated using Bristol medium: group I (control), no sodium selenite (Se); group II, 1 µM Se; group III, 10 µM Se; and group IV, 100 µM Se. Algal biomass samples were collected for biochemical evaluation and gene expression studies on the 21st day of cultivation. The following parameters were investigated: chlorophyll a (Cla), chlorophyll b (Clb) and total carotene content, total protein, and total glutathione (GSH) and malondialdehyde (MDA) levels. Gene expression levels of large subunits of Rubisco (rbcL) were analyzed using real-time quantitative polymerase chain reaction. Total Cla and total carotene in C. vulgaris decreased in high concentrations of Se (100 µM) (around 23 and 42%, respectively) when compared to controls while, Clb content increased by about 10%. 10 µM of Se led to increased GSH levels (3.04 ± 0.02 µg GSH/mg protein) and decreased MDA levels (2.02 ± 0.1 µmol MDA/mg protein) when compared to control groups (1.18 ± 0.04 µg GSH/mg protein and 0.94 ± 0.23 µmol MDA/mg protein), while a significant decrease in GSH and an increase in MDA levels in the presence of 100 µM Se showed the opposite effect. rbcL gene expression increased 1.76 ± 1.37-fold and 0.86 ± 1.33-fold in 10 and 100 µM selenium experiments when compared to control groups. Our results suggest both pro-oxidant and antioxidant activities of Se on C. vulgaris and upregulation of the rbcL gene for the first time. Treatment with low concentrations of Se improves the antioxidant features of the microalgae, C. vulgaris.

journal_name

3 Biotech

journal_title

3 Biotech

authors

Ozakman G,Yayman SG,Sezer Zhmurov C,Serdaroglu Kasikci E,Catal T

doi

10.1007/s13205-018-1212-4

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

189

issue

4

eissn

2190-572X

issn

2190-5738

pii

1212

journal_volume

8

pub_type

杂志文章

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