Photochemical analysis of structural transitions in DNA liquid crystals reveals differences in spatial structure of DNA molecules organized in liquid crystalline form.

Abstract:

:The anisotropic shape of DNA molecules allows them to form lyotropic liquid crystals (LCs) at high concentrations. This liquid crystalline arrangement is also found in vivo (e.g., in bacteriophage capsids, bacteria or human sperm nuclei). However, the role of DNA liquid crystalline organization in living organisms still remains an open question. Here we show that in vitro, the DNA spatial structure is significantly changed in mesophases compared to non-organized DNA molecules. DNA LCs were prepared from pBluescript SK (pBSK) plasmid DNA and investigated by photochemical analysis of structural transitions (PhAST). We reveal significant differences in the probability of UV-induced pyrimidine dimer photoproduct formation at multiple loci on the DNA indicative of changes in major groove architecture.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Brach K,Hatakeyama A,Nogues C,Olesiak-Banska J,Buckle M,Matczyszyn K

doi

10.1038/s41598-018-22863-z

subject

Has Abstract

pub_date

2018-03-14 00:00:00

pages

4528

issue

1

issn

2045-2322

pii

10.1038/s41598-018-22863-z

journal_volume

8

pub_type

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