Abstract:
:Influenza has been a long-running health problem and novel antiviral drugs are urgently needed. In pre-clinical studies, we demonstrated broad antiviral activity of D, L-lysine-acetylsalicylate glycine (LASAG) against influenza virus (IV) in cell culture and protection against lethal challenge in mice. LASAG is a compound with a new antiviral mode of action. It inhibits the NF-κB signal transduction module that is essential for IV replication. Our goal was to determine whether aerosolized LASAG would also show a therapeutic benefit in hospitalized patients suffering from severe influenza. The primary endpoint was time to alleviation of clinical influenza symptoms. The primary analysis was based on the modified intention-to-treat (MITT) population. This included all patients with confirmed influenza virus infection who received at least one treatment. The per protocol (PP) analysis set included all subjects from the MITT population who underwent at least 13 inhalations. In the MITT group, 48 (41.7%) participants (29 LASAG; 19 placebo) had severe influenza. The mean time to symptom alleviation was 56.2 h in the placebo group and 43.0 h in the LASAG group. The PP set consisted of 41 patients (24 LASAG; 17 placebo). The mean time to symptom alleviation in the LASAG group (38.3 h; P = 0.0365) was lower than that in the placebo group (56.2 h). In conclusion, LASAG improved the time to alleviation of influenza symptoms in hospitalized patients. The present phase II proof-of-concept (PoC) study demonstrates that targeting an intra-cellular signaling pathway using aerosolized LASAG improves the time to symptom alleviation compared to standard treatment.
journal_name
Emerg Microbes Infectjournal_title
Emerging microbes & infectionsauthors
Scheuch G,Canisius S,Nocker K,Hofmann T,Naumann R,Pleschka S,Ludwig S,Welte T,Planz Odoi
10.1038/s41426-018-0023-3subject
Has Abstractpub_date
2018-03-07 00:00:00pages
21issue
1issn
2222-1751pii
10.1038/s41426-018-0023-3journal_volume
7pub_type
杂志文章,多中心研究,随机对照试验abstract::The progressive anthropization of caves for food resources or economic purposes increases human exposure to pathogens that naturally infect cave-dwelling animals. The presence of wild or domestic animals in the immediate surroundings of caves also may contribute to increasing the risk of emergence of such pathogens. S...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2017.6
更新日期:2017-04-12 00:00:00
abstract::H7N9 influenza viruses emerged in 2013 and have caused severe disease and deaths in humans in China. Some H7N9 viruses circulating in chickens have mutated to highly pathogenic viruses that have caused several disease outbreaks in chickens. Studies have shown that when the H7N9 highly pathogenic viruses replicate in f...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0154-6
更新日期:2018-09-12 00:00:00
abstract::Saffold virus (SAFV), a newly discovered human cardiovirus of the Picornaviridae family, causes widespread infection among children, as shown by previous seroprevalence studies. To determine the host cell range of SAFV and its cytopathogenicity, eight mammalian cell lines that were available in the laboratory were scr...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2014.15
更新日期:2014-02-01 00:00:00
abstract::The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient's sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunos...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2016.114
更新日期:2016-11-09 00:00:00
abstract::Unlike those infected by H5N1, birds infected by the newly discovered H7N9 virus have no observable clinical symptoms. Public health workers in China do not know where the public health threat lies. In this study, we used a distributed focused crawler to analyze online knowledge of the live bird trade in first-wave pr...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2013.91
更新日期:2013-12-01 00:00:00
abstract::The recently emerged novel coronavirus, "severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)", caused a highly contagious disease called coronavirus disease 2019 (COVID-19). The virus was first reported from Wuhan city in China in December, 2019, which in less than three months spread throughout the globe and...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1080/22221751.2020.1775132
更新日期:2020-12-01 00:00:00
abstract::COVID-19, the worst pandemic in 100 years, has rapidly spread to the entire world in 2 months since its early report in January 2020. Based on the publicly available data sources, we developed a simple mathematic modeling approach to track the outbreaks of COVID-19 in the US and three selected states: New York, Michig...
journal_title:Emerging microbes & infections
pub_type: 信件
doi:10.1080/22221751.2020.1760146
更新日期:2020-12-01 00:00:00
abstract::From September 2015 to March 2016, hepatitis A notifications in Germany increased by 45% to 699 cases compared to 482 cases in the same period of the previous year. Children aged five to nine years were predominantly affected (22% of all cases in this period). We hypothesized that this increase could be explained by t...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2017.11
更新日期:2017-04-26 00:00:00
abstract::Molecular epidemiology of Clostridium difficile infection (CDI) has been extensively studied in North America and Europe; however, limited data on CDI are available in the Asia-Pacific region. A multicentre retrospective study was conducted in this region. C. difficile isolates were subjected to multilocus sequence ty...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1682472
更新日期:2019-01-01 00:00:00
abstract::The highly pathogenic avian influenza A (H5N6) virus has caused sporadic human infections with a high case fatality rate. Due to the continuous evolution of this virus subtype and its ability to transmit to humans, there is an urgent need to develop effective antiviral therapeutics. In this study, a murine monoclonal ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1708215
更新日期:2020-01-06 00:00:00
abstract::Acute meningoencephalitis (AME) is associated with considerable morbidity and mortality in children in developing countries. Clinical specimens were collected from children presenting with AME at two Cambodian paediatric hospitals to determine the major aetiologies associated with AME in the country. Cerebrospinal flu...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2017.15
更新日期:2017-05-24 00:00:00
abstract::Serology is a crucial part of the public health response to the ongoing SARS-CoV-2 pandemic. Here, we describe the development, validation and clinical evaluation of a protein micro-array as a quantitative multiplex immunoassay that can identify S and N-directed SARS-CoV-2 IgG antibodies with high specificity and sens...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1813636
更新日期:2020-12-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe gastroenteritis, which may lead to hemolytic uremic syndrome. The Locus of Enterocyte Effacement (LEE), a Pathogenicity Island (PAI), is a major determinant of intestinal epithelium attachment of a group of STEC strains; however, the ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1595985
更新日期:2019-01-01 00:00:00
abstract::The continuing pandemic threat posed by avian influenza A/H5N1 viruses calls for improved insights into their evolution during human infection. We performed whole genome deep sequencing of respiratory specimens from 44 H5N1-infected individuals from Indonesia and found substantial within-host viral diversity. At nearl...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1575700
更新日期:2019-01-01 00:00:00
abstract::Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal org...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0077-2
更新日期:2018-05-09 00:00:00
abstract::Tuberculosis (TB) has been a persistent public health concern for hundreds of years. Despite advances in medicine and science, eliminating this disease has been beyond our reach. Several organizations, including the National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Healt...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
doi:10.1038/emi.2012.21
更新日期:2012-07-01 00:00:00
abstract::Although a number of standardized human immunodeficiency virus 1 (HIV-1) pseudoviruses have been generated to assess neutralizing antibodies, subtype B/B' has not been comprehensively characterized either genotypically or phenotypically. Full-length env genes were isolated from plasma samples derived from B/B'-infecte...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0087-0
更新日期:2018-05-16 00:00:00
abstract::Interferon-induced transmembrane protein 3 (IFITM3) as an antiviral factor can inhibit replication of several viruses including influenza virus. A single-nucleotide polymorphism rs12252-C of IFITM3 results in a truncated IFITM3 protein lacking its first 21 amino acids, which is much higher in the Han Chinese populatio...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1756696
更新日期:2020-12-01 00:00:00
abstract::Clostridium difficile is the major causative agent of nosocomial antibiotic-associated diarrhea. In a 2009 outbreak of C. difficile-associated diarrhea that was recorded in a major Costa Rican hospital, the hypervirulent NAP1 strain (45%) predominated together with a local genotype variant (NAPCR1, 31%). Both strains ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2016.38
更新日期:2016-05-11 00:00:00
abstract::Serology detection is recognized for its sensitivity in convalescent patients with COVID-19, in comparison with nucleic acid amplification tests (NAATs). This article aimed to evaluate the diagnostic accuracy of serologic methods for COVID-19 based on assay design and post-symptom-onset intervals. Two authors independ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,meta分析
doi:10.1080/22221751.2020.1826362
更新日期:2020-12-01 00:00:00
abstract::Outbreaks of avian influenza virus continue to pose threats to human health. Animal models such as the mouse, ferret, and macaque are used to understand the pathogenesis of avian influenza virus infection in humans. We previously reported that the tree shrew (Tupaia belangeri, family Tupaiidae), which is regarded as a...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0167-1
更新日期:2018-10-10 00:00:00
abstract::Tick-borne encephalitis virus (TBEV) has been classified into three subtypes, namely the European (Eu-TBEV), Far Eastern (FE-TBEV), and Siberian (Sib-TBEV). In this study, we discovered a new subtype of TBEV in wild rodent Marmota himalayana in Qinghai-Tibet Plateau in China, proposed as subtype Himalayan (Him-TBEV). ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0081-6
更新日期:2018-04-25 00:00:00
abstract::Toxoplasmosis is one of the most common zoonotic diseases in the world. Felines excrete environmentally resistant Toxoplasma gondii oocysts. However, there is no direct evidence to prove tigers are the intermediate host of T. gondii. Here, we show that, IgG antibodies to T. gondii in 80% (8/10) of captive tigers. Two ...
journal_title:Emerging microbes & infections
pub_type: 信件
doi:10.1080/22221751.2019.1682471
更新日期:2019-01-01 00:00:00
abstract::We conducted this study to describe the dynamics of the acquisition of respiratory pathogens, their potential interactions and risk factors for possible lower respiratory tract infection symptoms (LRTI) among French pilgrims during the 2018 Hajj. Each participant underwent four successive systematic nasopharyngeal swa...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1693247
更新日期:2019-01-01 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with droplets and contact as the main means of transmission. Since the first case appeared in Wuhan, China, in December 2019, the outbreak has gradually spread nationwide. Up to now, acco...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1746199
更新日期:2020-12-01 00:00:00
abstract::Coxsackievirus A16 (CA16) is a member of the Picornaviridae family and causes mild and self-limiting hand, foot, and mouth disease (HFMD) in infants and young children. CA16 infection can also progress to central nervous system (CNS) complications; however, the underlying mechanism by which CA16 penetrates the blood-b...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0157-3
更新日期:2018-09-19 00:00:00
abstract::Shiga toxin (Stx)-producing Escherichia coli (STEC) can cause a wide range of symptoms from asymptomatic carriage, mild diarrhea to bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Intimin, encoded by the eae gene, also plays a critical role in STEC pathogenesis. Herein, we investigated the prevalence and gen...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2020.1850182
更新日期:2020-12-01 00:00:00
abstract::Human papillomaviruses (HPV) contribute to most cervical cancers and are considered to be sexually transmitted. However, papillomaviruses are often found in cancers of internal organs, including the stomach, raising the question as to how the viruses gain access to these sites. A possible connection between blood tran...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1637072
更新日期:2019-01-01 00:00:00
abstract::A 78-year-old woman with a long-term ankle spacer with antibacterials developed an intra-articular Candida auris infection. Treatment with systemic antifungal therapy plus an amphotericin B moulded cement spacer was successful. ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1625287
更新日期:2019-01-01 00:00:00
abstract::Tigecycline is regarded as a last-resort treatment for carbapenem-resistant Enterobacteriaceae (CRE), however, the emergence of tigecycline heteroresistance has posted the therapeutic challenge to combat this "nightmare bacteria". The primary purpose of this study was to demonstrate the existence of tigecycline hetero...
journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1601031
更新日期:2019-01-01 00:00:00