Abstract:
:Serology is a crucial part of the public health response to the ongoing SARS-CoV-2 pandemic. Here, we describe the development, validation and clinical evaluation of a protein micro-array as a quantitative multiplex immunoassay that can identify S and N-directed SARS-CoV-2 IgG antibodies with high specificity and sensitivity and distinguish them from all currently circulating human coronaviruses. The method specificity was 100% for SARS-CoV-2 S1 and 96% for N antigen based on extensive syndromic (n=230 cases) and population panel (n=94) testing that also confirmed the high prevalence of seasonal human coronaviruses. To assess its potential role for both SARS-CoV-2 patient diagnostics and population studies, we evaluated a large heterogeneous COVID-19 cohort (n=330) and found an overall sensitivity of 89% (≥ 21 days post onset symptoms (dps)), ranging from 86% to 96% depending on severity of disease. For a subset of these patients longitudinal samples were provided up to 56 dps. Mild cases showed absent or delayed, and lower SARS-CoV-2 antibody responses. Overall, we present the development and extensive clinical validation of a multiplex coronavirus serological assay for syndromic testing, to answer research questions regarding to antibody responses, to support SARS-CoV-2 diagnostics and to evaluate epidemiological developments efficiently and with high-throughput.
journal_name
Emerg Microbes Infectjournal_title
Emerging microbes & infectionsauthors
van Tol S,Mögling R,Li W,Godeke GJ,Swart A,Bergmans B,Brandenburg A,Kremer K,Murk JL,van Beek J,Wintermans B,Reimerink J,Bosch BJ,Reusken Cdoi
10.1080/22221751.2020.1813636subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1965-1973issue
1issn
2222-1751journal_volume
9pub_type
杂志文章abstract::Interleukin 17 (IL-17) and its closest relative, IL-17F, have recently drawn much attention in the field of immunology. IL-17 and IL-17F are expressed by a distinct type of T cells, T helper 17 cells and certain other lymphocytes. These cytokines play key regulatory roles in host defense and inflammatory diseases. In ...
journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
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journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
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journal_title:Emerging microbes & infections
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pub_type: 杂志文章
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1708215
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
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journal_title:Emerging microbes & infections
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pub_type: 杂志文章
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2016.20
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/emi.2013.71
更新日期:2013-10-01 00:00:00
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2019.1575700
更新日期:2019-01-01 00:00:00
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1080/22221751.2018.1564369
更新日期:2019-01-01 00:00:00
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journal_title:Emerging microbes & infections
pub_type: 杂志文章
doi:10.1038/s41426-018-0077-2
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Emerging microbes & infections
pub_type: 杂志文章,评审
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journal_title:Emerging microbes & infections
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