Abstract:
AIMS:Merkel cell carcinoma represents poorly differentiated neuroendocrine carcinoma of cutaneous origin. In most studies, the vast majority of Merkel cell carcinomas are Merkel cell polyomavirus (MCPyV)-associated. SV40 polyomavirus immunohistochemistry is typically used in the diagnosis of other polyomavirus-associated diseases, including tubulointerstitial nephritis and progressive multifocal leukoencephalopathy, given cross-reactivity with BK and JC polyomaviruses. MCPyV-specific immunohistochemistry is commercially available, but, if antibodies against SV40 also cross-reacted with MCPyV, that would be advantageous from a resource-utilisation perspective. METHODS AND RESULTS:Tissue microarrays were constructed from 39 Merkel cell carcinomas, 24 small-cell lung carcinomas, and 18 extrapulmonary visceral small-cell carcinomas. SV40 large T antigen immunohistochemistry (clone PAb416) was performed; MCPyV large T antigen immunohistochemistry (clone CM2B4) had been previously performed. UniProt was used to compare the amino acid sequences of the SV40, BK, JC and MCPyV large T antigens, focusing on areas recognised by the PAb416 and CM2B4 clones. SV40 immunohistochemistry was negative in all tumours; MCPyV immunohistochemistry was positive in 38% of Merkel cell carcinomas and in 0% of non-cutaneous poorly differentiated neuroendocrine carcinomas. UniProt analysis revealed a high degree of similarity between SV40, BK, and JC viruses in the region recognised by PAb416. There was less homology between SV40 and MCPyV in this region, which was also interrupted by two long stretches of amino acids unique to MCPyV. The CM2B4 clone recognises a unique epitope in one of these stretches. CONCLUSIONS:The PAb416 antibody against the SV40 large T antigen does not cross-react with MCPyV large T antigen, and thus does not label Merkel cell carcinoma.
journal_name
Histopathologyjournal_title
Histopathologyauthors
Pelletier DJ,Czeczok TW,Bellizzi AMdoi
10.1111/his.13483subject
Has Abstractpub_date
2018-07-01 00:00:00pages
162-166issue
1eissn
0309-0167issn
1365-2559journal_volume
73pub_type
杂志文章相关文献
HISTOPATHOLOGY文献大全abstract::Upper gastrointestinal endoscopic examination of 40 patients on immunosuppressive therapy after successful kidney transplantation revealed a duodenal ulcer in four cases and duodenal erosions in six. Between four and six biopsies were collected from the antral and the fundal mucosa in each case and from the duodenal m...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1982.tb02741.x
更新日期:1982-07-01 00:00:00
abstract::In this study breast tissue that contained ultrastructural dense core granules has been examined from 24 patients. The possible neuroendocrine characteristics of the granules were studied using the uranaffin reaction and an ultrastructural argyrophil stain. The tissue included 15 breast carcinomas and nine benign samp...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1987.tb01855.x
更新日期:1987-11-01 00:00:00
abstract::A Sertoli-Leydig cell tumour of testis presented some diagnostic difficulties. The tumour cells showed strong expression of S-100 antigen. Preliminary study of non-neoplastic testis suggests that Leydig cells and, to a lesser extent, Sertoli cells express S-100 antigen; its localization may be of value in the diagnosi...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1989.tb01634.x
更新日期:1989-12-01 00:00:00
abstract:AIMS:Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with alpha-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects. METHODS AND RESUL...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03553.x
更新日期:2010-05-01 00:00:00
abstract:AIMS:To look for FOXL2 mutation in rare ovarian epithelial lesions showing stromal components with morphological features of adult granulosa cell tumour (AGCT). METHODS AND RESULTS:We report the 402C→G FOXL2 mutation status in five epithelial ovarian lesions in women aged 45-77 years showing stromal proliferations tha...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12314
更新日期:2014-04-01 00:00:00
abstract:AIMS:Matrix metalloproteases (MMPs) and their inhibitors (TIMPs) play an essential role in the degradation of stromal connective tissue and basement membrane components. The aim of this study was to determine whether the dynamic analysis of these components can help to predict tumour aggressiveness. METHODS AND RESULT...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03723.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:Small cell (neuroendocrine) carcinoma of the urinary bladder is clinically more aggressive than urothelial (transitional cell) carcinoma. We have investigated the immunohistochemical markers most useful in diagnosing small cell carcinoma in bladder. METHODS AND RESULTS:We evaluated the expression of chromogranin ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1999.00715.x
更新日期:1999-08-01 00:00:00
abstract::Pancreatic acinar metaplasia of the gastric mucosa is a newly recognized entity. Its physiological relevance and association with other pathological conditions in the stomach remain to be elucidated. We studied by immunohistochemistry the expression of growth markers in the gastric mucosa in biopsies from 15 patients ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb00321.x
更新日期:1995-12-01 00:00:00
abstract:AIMS:Polymorphous low-grade adenocarcinoma (PLGA) is the second most common type of malignant neoplasm in minor salivary glands. Its origin in major salivary glands is considered exceedingly rare. Herein, we present three cases of de novo PLGA arising in major salivary glands. METHODS AND RESULTS:Three cases of PLGA w...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2004.01799.x
更新日期:2004-02-01 00:00:00
abstract:AIMS:To evaluate primary and recurrent embryonal sarcoma of the liver and to improve recognition of its morphological variants and immunohistochemical features. METHODS AND RESULTS:Fourteen primary and two recurrent cases of hepatic embryonal sarcoma were evaluated histologically and investigated immunohistochemically...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2007.02746.x
更新日期:2007-08-01 00:00:00
abstract::The clinical and pathological features of three adnexal tumours of probable Wolffian origin are reported. One case was an incidental finding in a patient who died from ovarian carcinoma; in the other two cases the patients presented with lower abdominal pain. The three tumours were well-circumscribed, solid masses ari...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb00623.x
更新日期:1995-01-01 00:00:00
abstract::Aneuploidy is an important prognostic factor in many cancers. Chromosome 1 abnormalities are present in most breast carcinomas. These may be part of a non-specific increase in DNA (aneuploid status) or represent a restricted chromosomal abnormality. In 16 breast carcinomas we compared chromosome 1 aneusomy with ploidy...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1997.d01-591.x
更新日期:1997-03-01 00:00:00
abstract:AIMS:We report a series of five unusual subcutaneous lipomas characterized by prominent stromal sclerosis in a storiform arrangement. METHODS AND RESULTS:All lesions occurred in young male adults (23.0 +/- 9.5 years), three lesions were on the scalp and two on the hands. Clinically, lipoma, chondroma or some form of c...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1997.2310836.x
更新日期:1997-08-01 00:00:00
abstract:AIMS:Most primary gastrointestinal lymphomas are of B-cell origin and T-cell origin is very rare. Recent studies have suggested that human T-cell lymphotrophic virus type 1 (HTLV-1) may be involved in the development of primary gastric T-cell lymphoma. We analysed 31 patients with primary gastric T-cell lymphoma in sou...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.2002.01459.x
更新日期:2002-11-01 00:00:00
abstract:AIMS:We have previously shown that hypertrophic scar tissue from burn patients contains abnormally high amounts of the proteoglycans versican and biglycan and reduced amounts of decorin, in comparison with normal dermis or mature scar. The lack of decorin may account for the poor organization of collagen fibrils in the...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.2000.00824.x
更新日期:2000-03-01 00:00:00
abstract::A retrospective histopathological study was undertaken to determine the prevalence of mucin filled ducts and their associated mucinous proliferation in 962 breast cancers and 335 benign lesions. A total of 38 (3%) cases with mucin filled ducts was identified and 27 (2%) of these showed mucin extravasation into the adj...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1996.d01-535.x
更新日期:1996-12-01 00:00:00
abstract:AIMS:Programmed death ligand 1 (PD-L1) immunohistochemistry has become a mandatory diagnostic test in the treatment of lung cancer. Several research initiatives have started to harmonise the five PD-L1 immunohistochemistry assays that have been used in clinical trials. Here, we report data on interlaboratory and intera...
journal_title:Histopathology
pub_type: 杂志文章,多中心研究
doi:10.1111/his.13375
更新日期:2018-02-01 00:00:00
abstract:AIMS:In some cases distinction between chromophobe renal cell carcinoma (CRCC), oncocytoma and clear cell (conventional) renal cell carcinoma (eosinophilic variant) using routine light microscopy remains problematic. The present study investigates the level of agreement in the diagnosis of CRCC, as well as the histolog...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2004.02003.x
更新日期:2004-12-01 00:00:00
abstract::Delta hepatitis (HDV) infection can only occur in the presence of hepatitis B (HBV) infection, as HDV requires a coat of HBV surface antigen (HBsAg) for assembly of complete virus. A number of studies have examined the variation of HBV markers in serum and liver during establishment of HDV infection, but none has syst...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1992.tb00990.x
更新日期:1992-04-01 00:00:00
abstract:AIMS:Comedo carcinoma is generally regarded as the subtype of ductal carcinoma in situ (DCIS) most likely to progress to invasive carcinoma. Increased angiogenesis could be associated with an enhanced risk of progression and might therefore be a marker of poor prognosis, as can be demonstrated for invasive breast tumou...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2005.02299.x
更新日期:2005-12-01 00:00:00
abstract:AIMS:It is still not clear whether oval cells demonstrate diverse morphology, immunophenotype or quantity in different human liver diseases. The aim of this study was to investigate these differences in hepatitis B virus (HBV)-positive and hepatitis C virus (HCV)-positive human liver cirrhosis (HLC). METHODS AND RESUL...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2006.02372.x
更新日期:2006-04-01 00:00:00
abstract:AIMS:In this study, we aimed to investigate the clinicopathological features of CD4+ and/or CD56+ immature haematolymphoid malignancy (iHLM), including blastic plasmacytoid dendritic cell neoplasm (BPDCN). METHODS AND RESULTS:We analysed the clinicopathological features of 46 patients diagnosed consecutively with CD4+...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13340
更新日期:2017-12-01 00:00:00
abstract::Granulomatous prostatitis may result from tuberculosis and fungal infection and has been described following prostatic surgery. In most cases, however, the aetiology is unknown, although it may be due to a reaction to extravasated or altered prostatic secretions. We have investigated cells (macrophages, lymphocytes), ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1991.tb00874.x
更新日期:1991-05-01 00:00:00
abstract:AIMS:Outcomes of colorectal cancer (CRC) treatment and survival have steadily improved during the past decades, accompanied by an increased risk of developing second primary tumours and metastatic tumours at unusual sites. Metastatic CRC can show mucosal colonisation, thereby mimicking a second primary tumour. This pot...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13892
更新日期:2019-09-01 00:00:00
abstract::A recently described unifying proposal for mantle cell lymphoma has led to the formulation of strict diagnostic criteria based on morphology, immunology and molecular data to define this specific entity. Previous studies were often based on broader definitions such as centrocytic lymphoma, intermediately differentiate...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb00615.x
更新日期:1995-01-01 00:00:00
abstract:AIMS:To assess the interobserver agreement in the reporting of colorectal polyps among histopathologists participating in the Welsh Bowel Cancer Screening (BCS) programme. METHODS AND RESULTS:Twelve benign polyps representative of BCS cases were identified from pathology files and reported by 28 BCS histopathologists ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12110
更新日期:2013-05-01 00:00:00
abstract:AIMS:To evaluate whether there is a correlation between the subcellular localization of maspin and the histological, molecular and biological features of pulmonary adenocarcinoma, particularly addressing the hypothesis that the tumour inhibitor properties of maspin may be linked to a nuclear, compared with a combined n...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2009.03260.x
更新日期:2009-04-01 00:00:00
abstract:AIMS:Rheumatoid arthritis (RA) and pigmented villonodular synovitis (PVNS) are aggressive diseases with progressive joint destruction. The present study aims to define cell cycle phases, polyploidy and the immunophenotype of proliferating synovial cells in both diseases. METHODS AND RESULTS:Synovial tissues from patie...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2005.01959.x
更新日期:2005-05-01 00:00:00
abstract::Calcitonin is a hypocalcaemia producing hormone and is secreted by C-cells of the thyroid. The current study was undertaken on a hypothesis that C-cell hyperplasia may develop in the secondary hyperparathyroidism of chronic renal failure in response to sustained hypercalcaemia. With an immunoperoxidase staining method...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1992.tb00433.x
更新日期:1992-11-01 00:00:00
abstract:AIMS:Sessile serrated adenoma/polyp (SSA/P) is regarded as a genetically homogeneous entity, with most lesions harbouring the BRAF V600E mutation. The present study aimed to reappraise the genetic heterogeneity of SSA/Ps and its clinicopathological significance. METHODS AND RESULTS:We performed next-generation sequenc...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13688
更新日期:2018-10-01 00:00:00